Patents Examined by Gail R Gabel
  • Diagnostic test for inflammatory endothelial dysfunctions in pregnancies
    Patent number: 9081018
    Abstract: The present invention relates to a diagnostic test for inflammatory endothelial dysfunctions in pregnant women. Particularly, the present invention relates to a method of diagnosing or evaluating the risk of contracting an inflammatory endothelial dysfunction of the maternal compartment comprising the following steps: a) detecting the plasma levels of long pentraxin PTX3 in blood samples taken from a pregnant woman; b) comparing the PTX3 plasma level data, obtained according to step a), with statistically significant PTX3 plasma level data of normal pregnant population.
    Type: Grant
    Filed: December 18, 2006
    Date of Patent: July 14, 2015
    Assignee: Humanitas Mirasole S.p.A.
    Inventors: Alberto Mantovani, Cecilia Garlanda, Andrea Doni, Irene Cetin
  • Cell-based detection of APF through its interaction with CKAP4 for diagnosis of interstitial cystitis
    Patent number: 9075045
    Abstract: An assay system designed to detect a protein biomarker in urine that is diagnostic for interstitial cystitis (IC). The presence of a 9 amino acid glycopeptide, antiproliferative factor (APF), in urine is unique to patients with IC. Urine samples from patients who exhibit symptoms consistent with IC are added to the assay system. Binding of APF to the cytoskeletal associated protein 4 (CKAP4) is positive for the presence of APF in urine and diagnostic for IC. The diagnostic system is a significant and surprising advance in diagnosis of IC and has commercial applications relevant to women and men who suffer from symptoms consistent with IC.
    Type: Grant
    Filed: June 6, 2013
    Date of Patent: July 7, 2015
    Assignees: The Commonwealth Medical College, University of Florida Research Foundation, Inc.
    Inventors: David Alan Zacharias, Sonia Lobo Planey
  • Method for detection of antigen-specific antibodies in biological samples
    Patent number: 9063150
    Abstract: Disclosed herein is a rapid and universal assay for the detection of antigen-specific antibodies in biological samples. The assay allows for the detection of antigen-specific antibodies in any species, including species for which secondary antibodies or antisera have not been developed or are not available. Biological samples to be tested are directly labeled, such as with biotin, and contacted with antigen-bound microparticles. The presence of antigen-specific antibodies in the biological samples is detected using a binding partner for the label, such as a biotin binding partner, conjugated to a detectable label, such as a fluorophore. This improved test provides a total antibody assay that is capable of detecting all classes of antibodies simultaneously.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: June 23, 2015
    Assignee: The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control Prevention
    Inventor: Alison Jane Basile
  • Multivalent opsonophagocytic assay using bead array and imaging
    Patent number: 9063145
    Abstract: Embodiments of the present invention relate to devices and methods for detecting opsonophagocytotic antibodies using detection particles having product-specific detection reagents and having a characteristic spectral feature. Detection substrates, include microwells. Phagocytic cells are grown in the microwells, and a sample of serum or other bodily fluid from a patient is introduced into the wells, along with a set of detection particles, each set having an antigen recognized by opsonophagocytotic antibodies. The detection particles bind to the antibodies present in the sample and the phagocytic cells then take up the detection particles, labeling the phagocytic cells. The cells are then visualized via image analysis, thereby detecting the presence of, and the types of the opsonophagocytotic antibodies in the sample.
    Type: Grant
    Filed: January 3, 2012
    Date of Patent: June 23, 2015
    Assignee: Cellular Technology Limited
    Inventor: Paul Lehmann
  • FACS-based method for obtaining an antibody sequence
    Patent number: 9057728
    Abstract: In certain embodiments, the method may comprises: a) contacting a population of permeabilized, cross-linked antibody-producing cells with a labeled antigen to produce a labeled sample in which cells that produce an antibody that specifically binds to said antigen are intracellularly labeled; b) using FACS to isolate cells that are intracellularly labeled, thereby producing labeled cells; c) uncrosslinking said labeled cells to produce uncrosslinked cells; and d) amplifying heavy and light chain-encoding nucleic acid from individual uncrosslinked cells, thereby obtaining nucleic acid that encodes the variable domain of antibody that specifically binds to said antigen.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: June 16, 2015
    Assignee: Epitomics, Inc.
    Inventors: Yaohuang Ke, Guo-Liang Yu, Karin Vroom
  • Immunoassay for thrombin detection
    Patent number: 9057737
    Abstract: The invention relates to an in vitro immunoassay for quantifying thrombin in a sample comprising anti-thrombin III (AT-III) and thrombin. The method comprises the following steps: contacting the sample with a small molecule that recognizes the substrate binding site of thrombin; contacting the thrombin with a thrombin specific antibody raised against a thrombin blocked in the active site; and measuring the level of bound antibody.
    Type: Grant
    Filed: November 30, 2011
    Date of Patent: June 16, 2015
    Assignee: Omrix Biopharmaceuticals Ltd.
    Inventors: Roni Mintz, Nadav Orr, Israel Nur
  • Isolation and/or identification of stem cells having adipocytic, chondrocytic and pancreatic differentiation potential
    Patent number: 9052316
    Abstract: The present invention relates to a method for isolating and/or identifying stem cells having adipocytic, chondrocytic and pancreatic differentiation potential, wherein an antibody is used that binds to the antigen TNAP, alone or in combination with an antibody that binds to the cell surface antigen CD56. The invention also relates to stem cells isolated by the method according to the invention for treating defects or damages or diseases in bone or cartilage of a patient in need thereof.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: June 9, 2015
    Assignee: EBERHARD-KARLS-UNIVERSITAET TUEBINGEN UNIVERSITAETSKLINIKUM
    Inventors: Hans-Joerg Buehring, Sabrina Treml, Reiner Lammers
  • Systems and methods for determining antibody-mediated risk index
    Patent number: 9052325
    Abstract: Systems and methods are provided for assessing the risk of hemolytic disease of the fetus or neonate, neonatal alloimmune thrombocytopenic purpura, or transfusion-associated lung injury in a patient or transfusion recipient.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: June 9, 2015
    Assignee: NEW YORK BLOOD CENTER, INC.
    Inventor: Gregory R. Halverson
  • Methods and compositions for treating bleeding disorders
    Patent number: 9046536
    Abstract: Aspects of the invention include methods for enhancing blood coagulation in a subject. In practicing methods according to certain embodiments, an amount of a non-anticoagulant sulfated polysaccharide (NASP) is administered to a subject to enhance blood coagulation in the subject. Also provided are methods for preparing a NASP composition having blood coagulation enhancing activity. Compositions and kits for practicing methods of the invention are also described.
    Type: Grant
    Filed: April 5, 2013
    Date of Patent: June 2, 2015
    Assignees: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Michael Dockal, Hartmut Ehrlich, Friedrich Scheiflinger
  • Pan-kinase activation and evaluation of signaling pathways
    Patent number: 9040249
    Abstract: Methods and reagents are provided for determining the activation state of a signal transduction pathway signaling protein. There exists a need in the art for methods that can monitor the efficacy of a signal transduction inhibitor in a patient. Other needs exist for detecting and monitoring certain disease or disorders that are associated with aberrant activation of a signal transduction pathway signaling protein. The present assay provides a highly sensitive assay that is also useful in patient populations in which obtaining a large cellular sample is difficult, for example, neonates.
    Type: Grant
    Filed: October 31, 2012
    Date of Patent: May 26, 2015
    Assignees: Beckman Coulter, Inc., University Health Network
    Inventors: David Hedley, Sue Chow, T. Vincent Shankey
  • Method for detecting substance in biological sample
    Patent number: 9034590
    Abstract: The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: May 19, 2015
    Assignees: JAPAN TOBACCO INC., VIRUS IKAGAKU KENKYUSHO INC.
    Inventors: Yoshimitsu Takakura, Naomi Oka, Kazuhiro Kondo
  • High throughput flow cytometry system and method
    Patent number: 9034257
    Abstract: The invention provides systems, compositions, kits and methods for automated processing of biological samples and analysis using a flow cytometer.
    Type: Grant
    Filed: October 27, 2009
    Date of Patent: May 19, 2015
    Assignee: Nodality, Inc.
    Inventors: Todd Covey, Adam Palazzo, Norman Purvis, James Cordiero, David Rosen
  • Signal amplification in lateral flow and related immunoassays
    Patent number: 9034656
    Abstract: The present invention provides methods, devices, compositions (e.g., capture complexes), and kits useful for enhancing the detection of antibodies in a test sample. The methods, devices, and compositions utilize detectable Fc-binding molecules such as Protein A, Protein G, and/or an Fc-specific antibody to amplify the signal of a detected antibody in immunoassays, such as lateral flow assays.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: May 19, 2015
    Assignee: ABAXIS, INC.
    Inventors: Rajesh K. Mehra, Kenneth P. Aron, Dennis M. Bleile, Jeremy Walker, Cristina Cuesico
  • Reagents for reducing leukocyte interference in immunoassays
    Patent number: 9018017
    Abstract: Methods and devices for reducing interference from leukocytes in an analyte immunoassay are provided. In one embodiment, a method is provided comprising the steps of amending a biological sample such as a whole blood sample with one or more leukocidal reagents that reduce or eliminate the metabolic activity of leukocytes, and performing an immunoassay on the amended sample to determine the concentration of analyte in the sample. Preferably, the sample is amended with one or more enzymes and optionally one or more enzyme substrates and cofactors.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: April 28, 2015
    Assignee: Abbott Point of Care, Inc.
    Inventors: John Lewis Emerson Campbell, Graham Davis, John Emegbero Omakor, Adam Roger Moss, Ganesh Guhados
  • Multi-sample particle analyzer and method for high throughput screening
    Patent number: 9012235
    Abstract: Embodiments of the present invention provide a system and method for analyzing a plurality of samples comprising obtaining with an autosampler a plurality of samples from a first plate having a plurality of sample wells wherein the autosampler has a plurality of probes for sampling a set of samples and wherein each probe of the plurality of probes is in communication with a separate flow cytometer via a separate conduit. The plurality of samples comprising particles is moved into a fluid flow stream for each separate conduit. Adjacent ones of the plurality of samples are separated from each other in the fluid flow stream by a separation gas, thereby forming a gas-separated fluid flow stream. The gas-separated fluid flow stream is independently guided to and through each separate flow cytometer.
    Type: Grant
    Filed: August 17, 2012
    Date of Patent: April 21, 2015
    Assignee: IntelliCyt Corporation
    Inventors: Linda Trinkle, R. Terry Dunlay, Bruce Edwards, Larry Sklar
  • Whole blood assay for measuring AMPK activation
    Patent number: 9005909
    Abstract: A method of sample analysis is provided. In certain embodiments, the method comprises: a) labeling cells of a blood sample using an antibody that specifically binds to phospho-AMPK or a phosphorylated target thereof, to produce a labeled sample; and b) measuring antibody binding by a population of blood cells of the labeled sample using flow cytometry. In particular embodiments, the method may further comprise, prior to the labeling step: contacting blood with a test agent ex vivo or in vivo; and comparing the evaluation to results obtained from a reference sample of blood cells.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: April 14, 2015
    Assignee: Rigel Pharmaceuticals, Inc.
    Inventors: Vadim Markovtsov, Yasumichi Hitoshi
  • Biological encoding of large numbers of cells
    Patent number: 8999652
    Abstract: Mixtures of cell types can be analyzed by having at least two signal markers, with at least one at three different levels to provide a barcode for each cell type. The mixture of cells may be subjected to a common candidate moiety and the effect of the moiety on the cells determined along with identification of the cell by the barcode. Conveniently, surface marker proteins and labeled antibodies can be used to create the barcode and the cells analyzed with flow cytometry.
    Type: Grant
    Filed: August 5, 2008
    Date of Patent: April 7, 2015
    Assignee: Primity Bio, Inc.
    Inventors: Peter Oliver Krutzik, Thomas Scott Wehrman
  • Reagent and kit for early diagnosis of kidney disease
    Patent number: 9000134
    Abstract: The invention provides reagents and methods for diagnosing kidney disease in a human or animal.
    Type: Grant
    Filed: May 12, 2008
    Date of Patent: April 7, 2015
    Inventors: Wallace B. Haigh, Donald L. Very, Jr.
  • Method for detecting membrane protein internalization
    Patent number: 8999653
    Abstract: The instant invention provides for methods for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell. More specifically, the methods involve (a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms, (b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest, (c) adding to the reaction medium (1) a modulating agent which is a fluorescent FRET acceptor compound compatible with the fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10?7M; or (2) a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: April 7, 2015
    Assignee: Cis-Bio International
    Inventors: Jurriaan Zwier, Robert Poole, Herve Ansanay, Michel Fink, Eric Trinquet
  • Post-translation modified cardiac troponin T as a biomarker of a risk for heart failure
    Patent number: 8986936
    Abstract: The present invention relates to methods and kits for the prediction of risk for heart failure using post-translation modified forms of cardiac troponin T as a biomarker.
    Type: Grant
    Filed: May 20, 2010
    Date of Patent: March 24, 2015
    Assignees: Inserm (Institut National de la Santa et de la Recherche Medicale), Institute Pasteur de Lille, Le Centre Hospitalier Regional Universitaire de Lille, Universite de Rouen
    Inventors: Florence Pinet, Paul Mulder, Christophe Bauters, Vincent Richard