Abstract: The purpose of the present invention is to develop a kit capable of detecting antigen or measuring its amount with high sensitivity without requiring an immobilization step and a washing step. The present invention provides a kit for detecting antigen or measuring its amount. The kit comprises a complex of a polypeptide including an antibody light chain variable region and a polypeptide including an antibody heavy chain variable region and a protein M fragment labeled with a fluorescent dye. The protein M fragment is a fragment having a binding ability to the complex.
Abstract: An object of the present invention is to provide a kit and a method capable of achieving high-precision measurement of a measurement target substance in a biological sample in a wide concentration range from a low concentration to a high concentration by sufficiently avoiding the influence of antibodies such as anti-serum albumin antibody present in blood.
Abstract: Provided is a method for efficiently searching a peptide library for an oligopeptide that can be bound to the end of a protein or peptide of interest. Further, provided is an efficient and highly safe immunoassay.
Abstract: The present disclosure relates to a Mitochondria-Associated endoplasmic reticulum Membrane (MAM)-specific fluorescence calcium sensor and the use thereof. The present disclosure can surmount the limitations of a conventional technique in that verification of calcium migration through MAM requires separate measurements of calcium ion concentrations within ER and mitochondria and situational explanations of the phenomena, and can directly measure concentrations in the paths through which calcium ions move to exclude influences on calcium ion changes through numerous different calcium ion channels existing in mitochondria, thereby providing a convenient and accurate MAM-specific calcium ion sensor.
Type:
Grant
Filed:
November 22, 2017
Date of Patent:
October 18, 2022
Assignee:
POSTECH ACADEMY—INDUSTRY FOUNDATION
Inventors:
Sang Ki Park, Yeong Jun Suh, Seung Hyun Kim
Abstract: Finding a protein of a minute amount present on a cell membrane to provide a method for producing an antibody against the protein. Producing an antibody using a protein identified by an identification method including: a labeling step of using a labeling agent comprising at least one selected from bis-iminobiotin compounds and bis-biotin compounds to obtain cells having a labeled protein; a degradation step of preparing a degradation product for an immobilization treatment, the degradation product containing the labeled protein; an immobilization step of immobilizing the labeled protein contained in the degradation product for an immobilization treatment on a stationary phase via a streptavidin mutant; a cleavage step of releasing an analysis sample from the stationary phase on which the labeled protein is immobilized; and an analysis step of analyzing the analysis sample to identify the labeled protein.
Type:
Grant
Filed:
February 19, 2018
Date of Patent:
September 13, 2022
Assignees:
MITSUI CHEMICALS, INC, NATIONAL INSTITUTES OF BIOMEDICAL INNOVATION HEALTH AND NUTRITION, SAVID THERAPEUTICS INC.
Abstract: The invention relates to the field of medical diagnostics. Provided are methods and kits for determining the health status of a subject, for early detection of tissue damage, for early diagnosis and monitoring of a disease, and/or for evaluation of treatment effectiveness in a subject using circulating tissue macrophages (CTM) as a mirror of disrupted tissue homeostasis and disease.
Type:
Grant
Filed:
January 14, 2020
Date of Patent:
September 13, 2022
Assignees:
Erasmus University Medical Center Rotterdam, Universidad De Salamanca
Inventors:
Jacobus Johannes Maria van Dongen, Jose Alberto Orfao de Matos Correia e Vale
Abstract: The present invention provides methods and compositions, e.g., kits, for assaying a vitamin D moiety in a sample, comprising or using, inter alia, a buffer that is capable of dissociating a vitamin D moiety from its binding protein and/or a buffer of acidic pH, and at least two antibodies, e.g., at least two monoclonal antibodies, that are separately conjugated to particles, e.g., latex particles, wherein at least one of said antibodies (or the first antibody) has a specific binding affinity towards the vitamin D moiety, and at least another said antibody (or the second antibody) has a specific binding affinity towards the complex formed between the first antibody and the vitamin D moiety, if present in said sample. In some embodiments, the optical change due to the agglutination reaction between the antibodies and the vitamin D moiety is measured for determination of the amount of vitamin D content in the samples. Kits and reaction mixtures for assaying a vitamin D moiety in a sample are also provided.
Type:
Grant
Filed:
July 28, 2017
Date of Patent:
September 6, 2022
Assignee:
Diazyme Laboratories, inc.
Inventors:
Chong-Sheng Yuan, Fakhri Ben Habib Saida
Abstract: The present invention relates to a device (100) for isolating an analyte (2) from a body fluid sample (4), wherein the analyte (2) is part of a target (6) contained in the body fluid sample (4). The device (100) comprises at least two types of magnetic particles (8, 12) for binding the analyte (2) and/or the target (6), at least three chambers (16, 18, 20) arranged in series to allow controlled movement of the magnetic particles (8, 12) and at least one fluid for releasing the analyte (2) from the target (6).
Type:
Grant
Filed:
November 23, 2017
Date of Patent:
September 6, 2022
Assignee:
Siemens Healthineers Nederland B.V.
Inventors:
Harma Martine Feitsma, Vanda Lucia De Carvalho Vittorino De Almeida, Christianne Rossette Maria De Witz, Matthias Irmscher, Sandra Martina Van Den Bosch
Abstract: The disclosure generally relates to the detection of symmetrical dimethylarginine (SDMA). More particularly, the disclosure relates to the detection of SDMA using a solid phase. The disclosure provides devices, reagents, kits and methods for detecting symmetrical dimethyl arginine (SDMA) in sample, such as a biological sample from an animal. The method includes detecting the presence or amount of SDMA in the sample by using an immunoassay format, such as a competitive immunoassay. The assay includes the use of antibodies to SDMA that are specific for SDMA and that have less affinity for other arginine derivatives.
Type:
Grant
Filed:
October 19, 2018
Date of Patent:
August 23, 2022
Assignee:
IDEXX Laboratories, Inc.
Inventors:
Hongzhi Xie, Sreenivasa Rao Ramisetty, Murthy V S N Yerramilli
Abstract: The invention discloses a method for reduction of autofluorescence in biological samples, comprising the steps of: a) providing a biological microscopy sample; b) irradiating the sample with visible light, wherein the visible light has a spectrum such that at least 50% of the light intensity emanates from a narrow wavelength interval within the visible range. The invention also discloses a method for autofluorescence reduction with triplet sensitizers irradiated with visible light.
Type:
Grant
Filed:
October 20, 2016
Date of Patent:
August 16, 2022
Assignee:
LEICA MICROSYSTEMS CMS GMBH
Inventors:
Anup Sood, Arunkumar Natarajan, Dmitry Dylov, Lakshmi Sireesha Kaanumalle, Elizabeth Mary McDonough
Abstract: The present invention relates to a reliable method of prediction of lower respiratory tract infection or inflammation in humans, wherein the level of pancreatic stone protein/regenerating protein (PSP/reg) is determined in serum, and a high level is indicative of the development and the severity of the disease, allowing the classification of patients according to risk.
Type:
Grant
Filed:
March 5, 2018
Date of Patent:
August 2, 2022
Assignees:
UNIVERSITY HOSPITAL OF BASEL, Lascco SA
Abstract: Methods are disclosed of designing antibodies for a sandwich assay for a small molecule having a molecular weight of about 500 to about 2,000. The method comprises preparing a first antibody that binds to the small molecule, and preparing a second antibody that binds to the small molecule at a portion of the small molecule other than a portion to which the first antibody binds. The second antibody is prepared from an immunogen that comprises a predetermined portion of the small molecule. The antibodies may be employed in sandwich assays for the small molecule.
Type:
Grant
Filed:
December 4, 2020
Date of Patent:
July 5, 2022
Assignee:
Siemens Healthcare Diagnostics Inc.
Inventors:
Yi Feng Zheng, Tie Q. Wei, Manoj Sharma
Abstract: A resin-metal composite 100 includes resin particles 10 and metal particles 20. The metal particles 20 are dispersed or immobilized on the resin particles 10, and portions of the metal particles 20 are three-dimensionally distributed in a surface section 60 of the resin particles 10. The metal particles 20 include encased metal particles 30 completely encased in the resin particles 10, partially exposed metal particles 40 having a portion embedded in a resin particle 10 and a portion exposed from the resin particle 10, and surface-adsorbed metal particles 50 absorbed on the surface of a resin particle 10.
Type:
Grant
Filed:
June 30, 2015
Date of Patent:
June 21, 2022
Assignee:
NIPPON STEEL Chemical & Material Co., Ltd.
Abstract: In certain embodiments, the present invention provides novel antibody purification methods and systems using a potentially simple and cost-efficient means. In some embodiments, customized Z-33 derived from Staphylococcus aureus Protein A is used to construct immuno-amphiphile molecules which can assemble into immunofibers in aqueous solution with bioactive epitopes on the surface and have IgG binding ability.
Type:
Grant
Filed:
March 28, 2018
Date of Patent:
June 14, 2022
Assignees:
THE JOHNS HOPKINS UNIVERSITY, BRISTOL-MYERS SQUIBB COMPANY
Inventors:
Honggang Cui, Yi Li, Xuankuo Xu, Lye Lin Lock, Zhengjian Li
Abstract: The invention provides a tandemly repeated protein comprising at least two repeats of an amino acid sequence of an antibody binding protein or a fragment thereof and a cell having the repeats expressed on the membrane thereof, which can be used in immunoassay to improve detection sensitivity and detection limit.
Type:
Grant
Filed:
July 13, 2017
Date of Patent:
May 3, 2022
Assignee:
TAIPEI MEDICAL UNIVERSITY
Inventors:
Kuo-Hsiang Chuang, Yi-Jou Chen, Michael Chen
Abstract: The present invention relates to a carrier for bio-related molecule immobilization, which comprises a resin substrate, an amino group-containing compound layer that is formed on the resin substrate, and a polyvalent carboxylic acid layer that is formed on the amino group-containing compound layer. The carboxyl groups in the polyvalent carboxylic acid layer are actively esterified; and the (COO peak intensity)/(C—C peak intensity) ratio of the C1s spectrum is from 0.064 to 0.12 (inclusive) if the carrier surface before the active esterification is measured by X-ray photoelectron spectroscopy.
Abstract: Methods, systems, compositions include biocompatible polymer coated nanoceria that function as aqueous redox catalyst with enhanced activity at an acidic to moderately alkaline pH value between 1 and 8. The compositions are used as oxidizing agents for decomposition, decontamination or inactivation of organic contaminants, such as, pesticides and chemical warfare agents. Another use includes nanoceria as targetable nanocatalyst prepared by conjugating various targeting ligands to the nanoparticle coating to form a colorimetric or fluorescent probe in immunoassays and other molecule binding assays that involve the use of a molecule in solution that changes the color of the solution or emits a fluorescent signal, where localization of nanoceria to organs or tissue is assessed by treatment with an oxidation sensitive dye or other detection devices.
Type:
Grant
Filed:
March 19, 2019
Date of Patent:
April 19, 2022
Assignee:
University of Central Florida Research Foundation, Inc.
Inventors:
Jesus Manuel Perez, Atul Asati, Santimukul Santra, Charalambos Kaittanis, Sudip Nath
Abstract: The present invention is directed to a pre-coated substrate, such as a slide, that is useful for immobilizing a sample. The invention is further provides methods of preparing such pre-coated substrates and methods of analyzing biological samples immobilized on such pre-coated substrate. The substrate is coated with a polycationic polymeric coating material specifically selected such that that coated substrate exhibits increased stability and prolonged shelf-life. Preferred polymeric coating materials include allylic or vinylic polymers having cationic groups thereon and having no more than a small percentage of peptidic monomeric linkages, particularly polydiallyldimethylammonium (PDDA).