Abstract: There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.
Type:
Grant
Filed:
May 26, 2017
Date of Patent:
December 10, 2019
Assignee:
DiaSorin S.p.A.
Inventors:
Joshua Soldo, Gregory Olson, Michael Lutterman, John Wall, Michael New, Hector Floyd Deluca, Fabrizio Bonelli
Abstract: Compounds of general formula I wherein Fc and Fc? may be the same or different and are substituted ferrocenyl moieties having at least one ring substituent selected from sulfur-containing groups, phosphorus-containing groups, iodo, chloro, silyl, fluoroalkyl groups containing two or more fluorine atoms, heteroaryl, substituted phenyl, and cyano, wherein if present as sole substituent the cyano group is located on the proximal cyclopentadienyl ring; X is a spacer. Y is a spacer, Z is a spacer; and R is a linker group. Compound I may be used to make labelled substrates, functionalised compounds for making labelled substrates and may be used as labels in an electrochemical assay.
Type:
Grant
Filed:
February 27, 2018
Date of Patent:
December 10, 2019
Assignee:
Atlas Genetics Limited
Inventors:
Barrie Marsh, Christopher Frost, David Pearce
Abstract: Compounds of general formula I are used as labels in an electrochemical assay: (I) in which: Fc and Fc? are substituted or unsubstituted ferrocenyl moieties, X is a C1 to C6 alkylene chain which is optionally interrupted by —O— or —NH—; Y is a C1 to C6 alkylene chain which is optionally interrupted by —O— or —NH—; Z is a C1 to C12 alkylene chain which may optionally be substituted and/or may optionally be interrupted by —O—, —S—, cycloalkyl, —CO—, —CON R1—, —NR1CO— or —NR1— in which R1 represents hydrogen or C1 to C4 alkyl; and R is a linker group. Compounds I are used to make labelled substrates, as well as functionalised compounds for making the labelled substrates.
Type:
Grant
Filed:
February 28, 2018
Date of Patent:
December 3, 2019
Assignee:
Atlas Genetics Limited
Inventors:
Barrie Marsh, Jonathan Sharp, Stephen Flower, Christopher Frost
Abstract: An immunoassay method is described which detects O-desmethyltramadol only. This enables an assay of high sensitivity and specificity avoiding false positive results. The unique antibodies incorporated in the immunoassay method can be combined with antibodies which detect mitragynine to provide an assay which increases the possibility of detecting the commonly found drug combination of O-desmethyltramadol and mitragynine.
Type:
Grant
Filed:
December 20, 2016
Date of Patent:
September 17, 2019
Assignee:
Randox Laboratories Limited
Inventors:
Ivan McConnell, Elouard Benchikh, Philip Lowry, Peter Fitzgerald
Abstract: Described herein are devices for capturing or isolating a biological cell from a sample, the device comprising a capture bed comprising a wave-herringbone surface pattern; and a plurality of nanostructures. Methods of making and using the same are also described.
Abstract: A preservative reagent for urine is disclosed that increases the stability of cells, such as tumor cells, in urine for a period of several weeks. The preservative reagent comprises polyethylene glycol (PEG), ethanol, paraformaldehyde (PFA), and ethylenediaminetetraacetic acid (EDTA), and optionally pH stabilizing reagents.
Type:
Grant
Filed:
March 13, 2014
Date of Patent:
August 6, 2019
Assignee:
CREATV MICROTECH, INC.
Inventors:
Peixuan Zhu, Daniel Adams, Cha-Mei Tang
Abstract: The present invention relates to a method of producing a solid coated carrier carrying biological material. Furthermore, the invention relates to a solid coated carrier to which biological material is attached and uses of the solid coated carrier for the preparation of a medical product. Moreover, the invention provides a method for the contacting, filtration or cleaning of blood, lymph or liquor cerebrospinalis of a patient, a method for the diagnosis of a disease and a diagnostic composition.
Abstract: Methods, compositions and kits are disclosed directed at posaconazole fragments, immunogens, signal generating moieties, antibodies that bind posaconazole and immunoassays for detection of posaconazole.
Type:
Grant
Filed:
February 10, 2014
Date of Patent:
March 20, 2018
Assignee:
ARK Diagnostics, Inc.
Inventors:
Johnny Valdez, Soon J. Oh, Byung Sook Moon