Patents Examined by Hope A. Robinson
  • Method of producing ginsenosides 20(S)-Rg3 and 20(S)-Rh2 using ginsenoside glycosidases
    Patent number: 10870857
    Abstract: The present invention relates to a method of producing ginsenoside 20(S)—Rg3 or 20(S)—Rh2 using a novel ginsenoside glycosidase in order to obtain ginsenoside 20(S)—Rg3 or 20(S)—Rh2 with high efficiency and high purity. According to the production method of the present invention, a large amount of 20(S)—Rg3 or 20(S)—Rh2, which is a minor form of rare ginsenoside present in very small amounts in ginseng or processed ginseng products, may be safely and efficiently produced. In particular, the method according to the present invention has an advantage in that it may produce a large amount of 20(S)—Rg3 or 20(S)—Rh2 for industrial applications, since the process is very simple and the production efficiency is very high.
    Type: Grant
    Filed: December 7, 2018
    Date of Patent: December 22, 2020
    Assignee: Modnbio, Inc.
    Inventors: Jong Cheon Hahm, Eun Kyung Do
  • Modified microorganism for the optimized production of 2,4-dihydroxybutyrate with enhanced 2,4-dihydroxybutyrate efflux
    Patent number: 10858675
    Abstract: The present invention relates to a recombinant microorganism capable of producing 2,4-dihydroxybutyrate, which is characterized by an increased cellular export, and preferably by a decreased cellular import, of said 2,4 DHB. The invention also relates to a method for the optimized production of 2,4-dihydroxybutyrate by culturing said microorganism in a fermentation medium and recovering 2,4-DHB from said medium.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: December 8, 2020
    Assignee: METABOLIC EXPLORER
    Inventors: Wanda Dischert, Laurence Dumon-Seignovert, Perrine Vasseur, Gwénaëlle Bestel-Corre, Philippe Soucaille
  • Spinosad heterologous expression strain and construction method thereof and use
    Patent number: 10844415
    Abstract: The invention provides a construction method of spinosad heterologous expression strain, a spinosad heterologous expression strain obtained by the method and use thereof in preparing spinosad. The method utilizes a plurality of homologous recombination to replace the erythromycin synthetic gene cluster of Saccharopolyspora erythraea with the spinosad synthetic gene cluster and the rhamnose synthetic gene cluster, such that the Saccharopolyspora erythraea produces spinosad.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: November 24, 2020
    Assignee: Zhejiang Hisun Pharmaceutical Co., LTD.
    Inventors: Jun Huang, Zhen Yu, Meihong Li, Linghui Zheng, Na Li, Haibin Wang, Hua Bai
  • Manganese supplementation for control of glycosylation in mammalian cell culture process
    Patent number: 10844416
    Abstract: The present invention pertains to a cell culture medium comprising manganese as a media supplement, which was shown to control recombinant protein glycosylation and methods of using thereof. The present invention further pertains to a method of controlling or manipulating glycosylation of a recombinant protein of interest in a large scale cell culture.
    Type: Grant
    Filed: June 1, 2016
    Date of Patent: November 24, 2020
    Assignee: Biogen MA Inc.
    Inventors: Lia Tescione, Thomas Ryll, Alan Gilbert
  • Arginine deiminase mutant methods of using the same
    Patent number: 10815470
    Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: October 27, 2020
    Assignee: Jiangnan University
    Inventors: Tao Zhang, Bo Jiang, Hangyu Jiang, Wanmeng Mu, Ming Miao
  • Method of treating hemophilia A
    Patent number: 10792336
    Abstract: There is provided a nucleic acid molecule comprising a nucleotide sequence encoding a Factor VIII protein, wherein a B domain portion of the Factor VIII protein is encoded by a nucleotide sequence between 90 and 111 nucleotides in length and has an amino acid sequence that is at least 85% identical to SEQ ID NO: 4 which comprises six asparagine residues. Also provided is a Factor VIII protein, a vector comprising the above nucleic acid molecule, a host cell, a transgenic animal, a method of treating Haemophilia Haemophilia A, and a method for the preparation of a parvoviral gene delivery vector.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: October 6, 2020
    Assignees: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, UCL BUSINESS LTD
    Inventors: Amit Nathwani, Jenny McIntosh, Edward Tuddenham, Andrew Davidoff
  • CBLB endonuclease variants, compositions, and methods of use
    Patent number: 10793843
    Abstract: The present disclosure provides improved genome editing compositions and methods for editing a CBLB gene. The disclosure further provides genome edited cells for the prevention, treatment, or amelioration of at least one symptom of, a cancer, an infectious disease, an autoimmune disease, an inflammatory disease, or an immunodeficiency.
    Type: Grant
    Filed: November 25, 2019
    Date of Patent: October 6, 2020
    Assignee: bluebird bio, Inc.
    Inventors: Jordan Jarjour, Kyle Havens, Anne-Rachel Krostag
  • L-threonine and L-tryptophan producing bacteria strain and method of making same
    Patent number: 10787692
    Abstract: The present invention relates to a microorganism able to produce L-threonine or L-tryptophan, and to a method for producing L-threonine or L-tryptophan by using same. More specifically, the present invention relates to: recombinant Escherichia coli which is more efficient in producing L-threonine or L-tryptophan by increasing the ability to produce ATP which is used as the most plentiful energy source in cells when producing L-threonine or L-tryptophan; and a method for producing L-threonine or L-tryptophan by using same.
    Type: Grant
    Filed: March 22, 2017
    Date of Patent: September 29, 2020
    Assignee: CJ Cheiljedang Corporation
    Inventors: Ki Yong Cheong, Seok Myung Lee, Young Bin Hwang, Keun Cheol Lee, Kwang Ho Lee
  • Process for extraction of oil from algal biomass
    Patent number: 10787625
    Abstract: Provided here is an enzymatic process for extraction of oil from algae biomass. The oils produced by the processes herein are used in animal feed and human consumption.
    Type: Grant
    Filed: September 26, 2017
    Date of Patent: September 29, 2020
    Assignee: Bunge Global Innovation LLC
    Inventors: Christopher L. G. Dayton, Ellen Cristina de Mello Lazarini, Jean Ricardo de Souza, Karen Carvalho, Eloisa Zanin Pytlowanciv, Bruna Montalvão Lima Ferraz
  • Compositions and methods for removing air pollutants
    Patent number: 10780394
    Abstract: Compositions for removing air pollutants from the air are provided. These compositions can be sprayed on a variety of surfaces to remove air pollutants such as volatile organic compounds (VOCs) from the environment, and are suitable for use in human dwellings.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: September 22, 2020
    Assignee: Honeywell International Inc.
    Inventors: Kai Huang, Marilyn Wang, Xingping Wang, Hailin Liu, Wesley Nie, Jack Yu
  • Method of screening compounds
    Patent number: 10767212
    Abstract: The present technology relates to kidney-type glutaminase (GLS) proteins that have a phenylalanine to tryptophan substitution at the position corresponding to residue 322 of human glutaminase C (GAC), and methods of using such proteins to screen for compounds that bind to the activation loop of GLS and/or modulate glutaminase activity of GLS.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: September 8, 2020
    Assignee: Cornell University
    Inventors: Richard Cerione, Jon W Erickson, Clint A Stalnecker
  • CYP76AD1-beta clade polynucleotides, polypeptides, and uses thereof
    Patent number: 10767201
    Abstract: The invention provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD6 or related gene and their use for producing L-DOPA from tyrosine and treating dopamine-responsive disorders, such as Parkinson's Disease. The invention also provides recombinant polynucleotides comprising a nucleic acid encoding a CYP76AD1 and/or CYP76AD6, a nucleic acid encoding a DOPA 4.5-dioxygenase (DOD) enzyme, such as Beta vulgaris DODA1, and, in some cases, a nucleic acid encoding betalain related glucosyltransferase, such as M. jalapa gene cyclo-DOPA 5-O-glucosyltransferase (cDOPA5GT), and their use for producing betalains. Finally, the invention provides chimeric polypeptides, expression vectors, cells, compositions, and organisms, including plants, and their uses in various methods of the invention.
    Type: Grant
    Filed: September 11, 2016
    Date of Patent: September 8, 2020
    Assignee: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
    Inventors: Asaph Aharoni, Guy Polturak
  • Engineered proline hydroxylase polynucleotides and methods
    Patent number: 10731189
    Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: August 4, 2020
    Assignee: Codexis, Inc.
    Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
  • Method for producing ACE2 polypeptide
    Patent number: 10716833
    Abstract: The present invention relates to recombinant ACE2 polypeptide, where the ACE2 polypeptide is present as a dimer. The dimer is formed specifically from glycosylated monomers and is used for producing pharmaceutical products with an extended half-life.
    Type: Grant
    Filed: December 6, 2017
    Date of Patent: July 21, 2020
    Assignee: APEIRON BIOLOGICS AG
    Inventors: Manfred Schuster, Hans Loibner, Evelyne Janzek-Hawlat, Bernhard Peball, Stefan Stranner, Bettina Wagner, Robert Weik
  • sp. microorganisms having L-lysine-producing ability and method for producing L-lysine using same
    Patent number: 10703783
    Abstract: The present invention relates to an L-lysine-producing microorganism of the genus Corynebacterium and a method for producing L-lysine using the same.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: July 7, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Seung Bin Lee, Yoon Hee Chung, Hyung Joon Kim, Doo Jin Kang, Seong Eun Bang, Song Gi Ryu
  • Method of producing heparan sulfate having anticoagulant activity
    Patent number: 10704068
    Abstract: The present invention provides a method of producing a heparan sulfate having an anticoagulant activity. Specifically, the present invention provides the method of producing a heparan sulfate having a desired molecular weight, by partially N-deacetylating a heparosan followed by depolymerizing the heparosan by heparinase III, and converting the produced low molecular weight product into heparan sulfate having an anticoagulant activity.
    Type: Grant
    Filed: June 26, 2018
    Date of Patent: July 7, 2020
    Assignee: AJINOMOTO CO., INC.
    Inventor: Yuriko Tokura
  • Engineered phytases and methods of using the same
    Patent number: 10687542
    Abstract: Methods for enhancing phytase thermal stability by fusing binding elements to target phytases are provided. Engineered phytases that include binding elements fused to target phytases to cause cyclization of the engineered phytases and enhance thermal stability of the target phytases are described. Engineered nucleic acids encoding engineered phytases and hosts engineered to express engineered nucleic acids are also provided. Methods for incorporating engineered phytases in animal feed and animal feed including the same are described.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: June 23, 2020
    Assignee: AGRIVIDA, INC.
    Inventors: R. Michael Raab, Gabor Lazar, Binzhang Shen
  • Methods for starch hydrolysis
    Patent number: 10689679
    Abstract: The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of ?-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of ?-amylase enzyme or is provided in the form of a purified or partially-purified ?-amylase enzyme preparation. The second or subsequent class(es) of ?-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified ?-amylase enzyme preparation.
    Type: Grant
    Filed: July 23, 2019
    Date of Patent: June 23, 2020
    Assignee: Syngenta Participations AG
    Inventor: George W. Aux
  • Detergent composition
    Patent number: 10689603
    Abstract: A detergent composition including an oleic acid-transforming enzyme and a surfactant system. Furthermore, a method of manually washing articles including the steps of: delivering a composition including an oleic acid-transforming enzyme and a surfactant system to a volume of water to form a wash liquor and immersing the soiled articles, in the liquor.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: June 23, 2020
    Assignee: The Procter & Gamble Company
    Inventors: Neil Joseph Lant, Jean-Luc Philippe Bettiol, Denis Alfred Gonzales
  • Method of making variant LovD polypeptides
    Patent number: 10689628
    Abstract: The invention disclosed herein relates to methods and materials for producing simvastatin and related compounds such as huvastatin. In particular, the disclosure teaches that variants of the LovD acyltransferase polypeptide can be engineered to exhibit properties that facilitate their use in the production of simvastatin and/or huvastatin. The materials and processes disclosed herein are designed so that fermentation facilitates lovastatin being converted to simvastatin and related compounds with minimal modifications.
    Type: Grant
    Filed: February 15, 2019
    Date of Patent: June 23, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Yi Tang, Xue Gao, Xinkai Xie