Abstract: The present invention features a number of methods for identifying one or more compounds that bind to a biological target. The methods include synthesizing a library of compounds, wherein the compounds contain a functional moiety having one or more diversity positions. The functional moiety of the compounds is operatively linked to an initiator oligonucleotide that identifies the structure of the functional moiety.
Abstract: Methods of uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are provided. Kits for uniquely labeling or barcoding molecules within a cell, a plurality of cells, and/or a tissue are also provided. The molecules to be labeled may include, but are not limited to, RNAs, cDNAs, DNAs, proteins, peptides, and/or antigens.
Type:
Grant
Filed:
February 25, 2021
Date of Patent:
November 9, 2021
Assignee:
UNIVERSITY OF WASHINGTON
Inventors:
Georg Seelig, Richard Muscat, Alexander B. Rosenberg
Abstract: Metagenomic data are increasingly useful for detecting microbes, such as viruses and other microorganisms in a wide range of sample types. Methods and systems for isolating, detecting, and characterizing microbes in biological samples using metagenomic data are provided. Also disclosed are methods for identifying microbes in nucleic acid libraries.
Abstract: Embodiments described herein are related to a one-step surface modification method with 3-aminopropyl trimethoxysilane (APTMS), which was developed and applied for DNA immobilization on a paper-based device via the ionic adsorption.
Type:
Grant
Filed:
November 29, 2017
Date of Patent:
October 26, 2021
Assignee:
THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
Abstract: A method for evaluating and screening a mutant inducing the high-efficiency formation of heterodimers from a human antibody heavy chain constant region mutant pair combination library in order to increase the yield of formation of human antibody heterodimeric heavy chain constant regions. A heterodimeric heavy chain constant region (heterodimeric F) library is obtained by the method. A CH3 domain mutant pair, in which the formation of the heterodimeric heavy chain constant regions is preferred in the library, forms heterodimeric heavy chain constant regions at a high yield of at least 80-90%, and also has excellent thermal stability and retains binding ability to the heavy chain constant region receptor (FcRn).
Type:
Grant
Filed:
October 12, 2016
Date of Patent:
October 26, 2021
Assignee:
AJOU UNIVERSITY INDUSTRY—ACADEMIC COOPERATION FOUNDATION
Abstract: The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: a forward fusion primer designed according to the target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.
Type:
Grant
Filed:
March 28, 2018
Date of Patent:
October 26, 2021
Assignee:
Genetron Health (Beijing) Co., Ltd.
Inventors:
Hai Yan, Sizhen Wang, Yuchen Jiao, Dayong Xu, Qiaosong Zheng, Xiao Shi
Abstract: Methods of sequencing and assembling a nucleic acid sequence from a nucleic acid sample containing repetitive or low-information regions, which are typically difficult to sequence and/or assemble are provided. The methods of sequencing and assembling introduce mutations into the sample to increase sequence diversity between various repetitive regions present in the nucleic acid sample. This sequence diversity allows various segments to assemble independently of different, but similar sequences present in the nucleic acid sample.
Type:
Grant
Filed:
October 28, 2019
Date of Patent:
October 26, 2021
Assignee:
The Board of Trustees of the Leland Stanford Junior University
Inventors:
Solomon Endlich, Devin King, Ashby J. Morrison
Abstract: A sequencing capture array for identifying mutations in Multiple Myeloma is disclosed. Also disclosed are targeted next generation sequencing methods for identifying SNV, CNV, and translocation mutations in Multiple Myeloma tumor cells. A capture array representing fewer than 500 genes implicated in Multiple Myeloma can be used to analyze tumor mutations and create a personalized treatment plan for a Multiple Myeloma patient. Analytical methods are presented that allow tumor mutations to be elucidated with coverage at a sequencing depth of no more than 500×, or as low as 100×, with optimal efficiency achieved at a sequencing depth of about 300×.
Type:
Grant
Filed:
November 6, 2017
Date of Patent:
October 12, 2021
Assignee:
Washington University
Inventors:
Brian White, Irena Lanc, Robert Fulton, Daniel Auclair, Michael H. Tomasson
Abstract: Novel methods for making high resolution oligonucleotide paints are provided. Novel, high resolution oligonucleotide paints are also provided.
Type:
Grant
Filed:
October 6, 2017
Date of Patent:
September 28, 2021
Assignee:
President and Fellows of Harvard College
Inventors:
Chao-ting Wu, George M. Church, Benjamin Richard Williams
Abstract: The invention provides methods for non-invasive prenatal testing that allow for detecting risk of chromosomal and subchromosomal abnormalities, including but not limited to aneuploidies, microdeletions and microduplications, insertions, translocations, inversions and small-size mutations including point mutations and mutational signatures. The methods of the invention utilize a pool of TArget Capture Sequences (TACS) to enrich for sequences of interest in a mixed sample containing both maternal and fetal DNA, followed by massive parallel sequencing and statistical analysis of the enriched population to thereby detect the risk of a genetic abnormality in the fetal DNA. Kits for carrying out the methods of the invention are also provided.
Type:
Grant
Filed:
May 20, 2016
Date of Patent:
September 7, 2021
Assignee:
NIPD GENETICS PUBLIC COMPANY LTD
Inventors:
George Koumbaris, Elena Kypri, Kyriakos Tsangaras, Achilleas Achilleos, Petros Mina, Elisavet A. Papageorgiou, Philippos C. Patsalis
Abstract: The present invention relates to improved yeast transformation of yeast cells and yeast cell libraries transformed thereby. More specifically, the present invention relates to the transformation of yeast by electroporation.
Type:
Grant
Filed:
May 8, 2020
Date of Patent:
August 31, 2021
Assignee:
IMMATICS BIOTECHNOLOGIES GMBH
Inventors:
Sebastian Bunk, Dominik Maurer, Felix Unverdorben
Abstract: The present invention is related to an in vitro method for diagnosing Gaucher's disease in a subject comprising a step of a) detecting a biomarker in a sample from the subject, wherein the biomarker is free lyso-Gb1.
Abstract: The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.
Type:
Grant
Filed:
February 26, 2019
Date of Patent:
August 10, 2021
Assignee:
President and Fellows of Harvard College
Inventors:
George M. Church, Evan R. Daugharthy, Richard C. Terry, Benjamin W. Pruitt, Brian M. Turczyk
Abstract: A method of producing a cDNA from a sample of nucleic acids includes the steps of: generating a dephosphorylated RNA from the sample; ligating the dephosphorylated RNA with a first adapter in the presence of a crowding agent to produce a ligated product; removing the excess first adapter by adding at least two enzymes; and performing reverse transcription in a lithium-containing buffer to produce the cDNA. A method of preparing a DNA library and a kit for such preparation are also disclosed.
Abstract: Methods and techniques to identify carcinogenesis pathways and markers for early cancer diagnosis. Cell sampling is performed on a single tumor with multiple samples being taken from the tumor and outward toward the periphery and beyond. Large scale analysis is performed, such as whole genomic sequencing, to identify the differences between the cells of the various samples. The differences are evaluated to determine which differences represent a change along the carcinogenesis pathway.
Abstract: The present invention provides biomarker compositions and methods for the diagnosis and prognosis of PDAC. In a particular embodiment, the invention provides methods and compositions for screening, diagnosis and prognosis of early stage, asymptomatic PDAC.
Abstract: Described herein is a population of direct repeat molecules, where each molecule of the population contains a direct repeat composed of sequences that are amplified from the opposite strands of a double-stranded fragment of genomic DNA. Within each molecule, the first repeat (referred to as TOP) is amplified from the one strand of a double-stranded fragment of genomic DNA and the second repeat (referred to as BOT?) is amplified from the other strand of the same fragment of double-stranded fragment of genomic DNA.
Abstract: Methods of monitoring and measuring dynamic adaptive immune cell responses are provided. High-throughput sequencing of T cell receptor and immunoglobulin loci is used to characterize the breadth of an effector cell response to a stimulus, such as a vaccine or infection. Unique responding effector cell clones and abundance thereof can be determined. Additionally, methods for determining the contribution of responding effector cells to the immunological memory compartment are provided.
Type:
Grant
Filed:
November 24, 2015
Date of Patent:
July 20, 2021
Assignees:
Adaptive Biotechnologies Corporation, Fred Hutchinson Cancer Research Center
Inventors:
Harlan S. Robins, William Sumner DeWitt, III, Ryan O. Emerson
Abstract: The present invention is directed to SH3 domain derivatives having a specific binding affinity to a target molecule. In this respect, the invention provides SH3 domain derivatives of nephrocystin (NPHP1) and the Tec kinase. The invention also provides a method for the production of a library comprising recombinant derivatives of NPHP1 or the Tec kinase SH3 domains and a method for selecting from the library one or more derivatives of the SH3 domain of nephrocystin (NPHP1) or the Tec kinase having a specific binding affinity to a target molecule.