Abstract: The present invention relates to monodisperse silanized ferrimagnetic iron oxide particles, a method for producing the same and a method for independent generic binding of nucleic acid molecules to the particles.
Type:
Grant
Filed:
August 21, 2018
Date of Patent:
April 27, 2021
Assignee:
Roche Molecular Systems, Inc.
Inventors:
Jens Christian Bolle, Thomas Walter, Peter Wenzig
Abstract: The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
Abstract: Some embodiments described herein relate to modified nucleotide and nucleoside molecules with novel 3?-hydroxy protecting groups. Also provided herein are methods to prepare such modified nucleotide and nucleoside molecules and sequencing by synthesis processes using such modified nucleotide and nucleoside molecules.
Type:
Grant
Filed:
September 6, 2019
Date of Patent:
April 20, 2021
Assignee:
Illumina Cambridge Limited
Inventors:
Xiaohai Liu, Xiaolin Wu, Geoffrey Paul Smith
Abstract: The present invention relates to a quencher having a quenching effect on a fluorescent material exhibiting luminescence characteristics at an excited energy level, and various uses thereof.
Type:
Grant
Filed:
November 23, 2018
Date of Patent:
April 20, 2021
Assignee:
SFC CO., LTD.
Inventors:
Do-Min Lee, Seung-Soo Lee, Jin-Hee Park, Sun-Ho Kim, Goutam Masanta, Jong-Tae Je
Abstract: The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5?-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis.
Type:
Grant
Filed:
March 18, 2020
Date of Patent:
April 13, 2021
Assignee:
Roche Sequencing Solutions, Inc.
Inventors:
Dmitriy Gremyachinskiy, Meng Taing, Aruna Ayer, Peter J. Crisalli
Abstract: A novel assay and a suite of devices may isolate nucleic acids from prokaryotic and eukaryotic cells and prepare samples for real-time (quantitative) polymerase chain reaction (PCR) analysis. The assay may employ an aqueous-based non-alcohol approach that yields robust RNA quality. The suite of ready-to-use devices may provide pre-loaded reagents in liquid and lyophilized formats to enable rapid manual operation in a laboratory or remote field environments. The assay and devices may be particularly suitable to analysis in microgravity or deep space environments.
Type:
Grant
Filed:
August 28, 2017
Date of Patent:
April 13, 2021
Assignee:
United States of America as Represented by the Administrator of NASA
Inventors:
Travis David Boone, Jimmy Kar Chuen Jung, Macarena Parra, Mark Brown
Abstract: The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.
Type:
Grant
Filed:
August 26, 2016
Date of Patent:
April 13, 2021
Assignee:
Roche Sequencing Solutions, Inc.
Inventors:
Frank Bergmann, Christoph Seidel, Andrew Trans, Dmitriy Gremyachinskiy, Hannes Kuchelmeister, Lars Hillringhaus
Abstract: The present invention provides a method of amplifying an RNA molecule in a biological sample by reverse transcription PCR (RT-PCR), wherein the RT-PCR is carried out in a solution comprising a polar aprotic solvent; a serum albumin, and a polyol.
Type:
Grant
Filed:
December 18, 2015
Date of Patent:
April 6, 2021
Assignee:
Global Life Sciences Solutions Operations UK Ltd
Inventors:
Alexander Yarunin, Peter James Tatnell, Kathryn Louise Lamerton, Rebecca Ngaire Fullerton
Abstract: The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.
Abstract: Light harvesting luminescent multichromophores that are configured upon excitation to transfer energy to, and amplify the emission from, an acceptor signaling chromophore in energy-receiving proximity therewith are provided. Also provided are compositions for labelling a target. The labelling composition may include a donor light harvesting multichromophore and an acceptor signaling chromophore in energy-receiving proximity to the donor light harvesting multichromophore. Also provided is an aqueous composition for labelling a target, including: a donor light harvesting multichromophore; an acceptor signaling chromophore in energy-receiving proximity therewith; and a sensor biomolecule. Methods for using the subject compositions are also provided.
Type:
Grant
Filed:
February 26, 2020
Date of Patent:
March 16, 2021
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventors:
Guillermo C. Bazan, Brent S. Gaylord, Shu Wang, Bin Liu
Abstract: The invention relates to a method for isolation of nucleic acids from aqueous samples containing nucleic acids in free form or liberated by lysis. Before or after the polarity of the aqueous solution is lowered, the sample is brought into contact with a solid phase that has a rough or structured surface, whereupon the nucleic acids precipitate on the solid phase and then, together with the solid phase, are removed from this aqueous solution. The rough or structured surface is preferably a non-smooth metal, plastic or rubber surface. Subject matter of the invention is also a test kit and a corresponding instrument for isolation of nucleic acids.
Abstract: Oligonucleotide analogues are provided that incorporate 5-aza-cytosine in the oligonucleotide sequence, e.g., in the form of 5-aza-2?-deoxycytidine (decitabine) or 5-aza-cytidine. In particular, oligonucleotide analogues rich in decitabine-deoxyguanosine islets (DpG and GpD) are provided to target the CpG islets in the human genome, especially in the promoter regions of genes susceptible to aberrant hypermethylation. Such analogues can be used for modulation of DNA methylation, such as effective inhibition of methylation of cytosine at the C-5 position. Methods for synthesizing these oligonucleotide analogues and for modulating nucleic acid methylation are provided. Also provided are phosphoramidite building blocks for synthesizing the oligonucleotide analogues, methods for synthesizing, formulating and administering these compounds or compositions to treat conditions, such as cancer and hematological disorders.
Abstract: Provided are methods for site selective conjugation of an oligonucleotide conjugate to a metal binding protein comprising a metal binding site and for site selective conjugation of a small molecule conjugation compound (SMCoC) to an antibody comprising a metal binding site, metal binding protein conjugates obtainable by said methods, and uses of said metal binding protein conjugates.
Type:
Grant
Filed:
July 8, 2015
Date of Patent:
February 23, 2021
Assignee:
Affycon ApS
Inventors:
Kurt Vesterager Gothelf, Thomas Torring, Christian Bech Rosen, Anne Louise Bank Kodal, Michael Rosholm Mortensen
Abstract: The present disclosure relates to methods, compositions, and kits for treating target nucleic acids, including methods and compositions for fragmenting and tagging nucleic acid (e.g., DNA) using transposome complexes bound to a solid support.
Type:
Grant
Filed:
February 20, 2018
Date of Patent:
February 16, 2021
Assignees:
ILLUMINA, INC.
Inventors:
Grace DeSantis, Stephen M. Gross, Jian-Sen Li, Natalie Morrell, Andrew Slatter, Kevin Shen, Samantha Snow
Abstract: This invention provides a process for making 3?-O-allyl-dGTP-PC-Biodopy-FL-510, 3?-O-allyl-dATP-PC-ROX, 3?-O-allyl-dCTP-PC-Bodipy-650 and 3?-O-allyl-dUTP-PC-R6G, and related methods.
Type:
Grant
Filed:
January 11, 2016
Date of Patent:
February 2, 2021
Assignee:
The Trustees of Columbia University in the City of New York
Inventors:
Jingyue Ju, Qinglin Meng, Dae H Kim, Lanrong Bi, Xiaopeng Bai, Nicholas J Turro
Abstract: A method of detection and identification of one or more microorganism/s in a biological sample comprising the following steps: (a) extracting DNA from the microorganism/s; and (b) amplifying the extracted DNA and indicating the level of extracted DNA in a quantitative PCR; wherein the quantitative PCR is performed using the primer pair of SEQ ID NO. 1 and 2 together with the probe of SEQ ID NO. 7, and/or the primer pair of SEQ ID NO. 3 and 4 together with the probe of SEQ ID NO. 8.
Abstract: The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.
Abstract: The disclosed invention teaches processes to create a Watson-Crick complementary copy of a preselected oligonucleotide that contain non-standard nucleotides, which form nucleobase pairs fitting the standard Watson-Crick geometry, but here said pairs are joined by hydrogen bonding patterns different from those that join standard A:T and G:C pairs. The invention further relates to polymerases that incorporate those non-standard nucleotide analogs into oligonucleotide products using the corresponding triphosphate derivatives.
Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:
Abstract: Disclosed is a process for isolating cell-free nucleic acid (including both DNA and RNA) or an analog thereof from a bodily fluid, and which entails: a) mixing in a container the bodily fluid, a chaotropic agent in solid form, a detergent and a buffer, and a solid phase which includes magnetic particles, thus forming a reaction mixture containing the cell-free nucleic acid; b) magnetically separating the solid phase having the cell-free nucleic acid bound thereto from the reaction mixture; and optionally c) dissociating the nucleic acid from the solid phase. Compositions and kits are also disclosed.