Abstract: The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample and for stabilizing the transcriptome of contained cells.
Type:
Grant
Filed:
September 25, 2013
Date of Patent:
July 28, 2020
Assignee:
Qiagen GmbH
Inventors:
Martin Horlitz, Annabelle Schubert, Markus Sprenger-Haussels, Kalle Günther, Ralf Wyrich, Uwe Oelmüller
Abstract: A fluorescent probe for binding to and detection of AP sites of DNA includes the following formula: F-L-X where F is a fluorescent moiety, X is an aminooxy group (—ONH2), and L is a linker that links or couples the fluorescent moiety to the oxyamine.
Type:
Grant
Filed:
April 14, 2016
Date of Patent:
July 21, 2020
Assignee:
CASE WESTERN RESERVE UNIVERSITY
Inventors:
Stanton L. Gerson, Yanming Wang, Allison G. Condie
Abstract: A kit for determining ctDNA concentration and a method for determining ctDNA concentration is disclosed, wherein the method comprising dissolving extracted ctDNA in Tris-HCl buffer with pH>7.0 and adding into the buffer 2,7-bis(1-methyl-4-vinylpyridine)-9-ethylcarbazole iodised salt as a molecular probe, calculating the concentration of ctDNA in the solution by measuring the Fluorescence intensity of the solution. It has extremely high sensitivity in the determination of 0 ?g/ml˜50 ?g/ml ctDNA solution.
Type:
Grant
Filed:
April 3, 2018
Date of Patent:
July 14, 2020
Inventors:
Wenfeng Yu, Shi Zhou, Zhi Huang, Bi Wang, Rui Gao, Dai Wei Zhao, Hai Zhuang, Ya Ling Liang
Abstract: A method of detection and identification of one or more microorganism/s in a biological sample comprising the following steps: (a) extracting DNA from the microorganism/s; and (b) amplifying the extracted DNA and indicating the level of extracted DNA in a quantitative PCR; wherein the quantitative PCR is performed using the primer pair of SEQ ID NO. 1 and 2 together with the probe of SEQ ID NO. 7, and/or the primer pair of SEQ ID NO. 3 and 4 together with the probe of SEQ ID NO. 8.
Abstract: Cyclic-GMP-AMP synthase (cGAS) and cyclic-GMP-AMP (cGAMP), including 2?3-cGAMP, 2?2-cGAMP, 3?2?-cGAMP and 3?3?-GAMP, are used in pharmaceutical formulations (including vaccine adjuvants), drug screens, therapies, and diagnostics.
Type:
Grant
Filed:
December 13, 2019
Date of Patent:
June 30, 2020
Assignee:
THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
Abstract: Provided herein is technology relating to sequencing nucleic acids, but not exclusively, to compositions, methods, systems, and kits related to nucleotides comprising an electrochemically detectable moiety and one or more photolabile synthesis-inhibiting moieties.
Type:
Grant
Filed:
August 12, 2019
Date of Patent:
June 30, 2020
Assignee:
IBIS BIOSCIENCES INC.
Inventors:
Mark A. Hayden, Jeffrey Huff, Mark W. Eshoo, John Picuri
Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.
Abstract: The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using renewable initiators coupled to a solid support. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template.
Abstract: The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using an apoptosis inhibitor, preferably a caspase inhibitor, a hypertonic agent and/or a compound according to formula (1) as defined in the claims.
Type:
Grant
Filed:
September 25, 2012
Date of Patent:
June 9, 2020
Assignee:
QIAGEN GMbH
Inventors:
Martin Horlitz, Annabelle Schubert, Markus Sprenger-Haussels
Abstract: Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.
Type:
Grant
Filed:
May 19, 2017
Date of Patent:
June 9, 2020
Assignee:
Pacific Biosciences of California, Inc.
Inventors:
Gene Shen, Stephen Yue, Lubomir Sebo, Louis Brogley
Abstract: Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
Type:
Grant
Filed:
November 20, 2017
Date of Patent:
June 9, 2020
Assignee:
Bio-Rad Laboratories, Inc.
Inventors:
Adam Lowe, Geoff McDermott, Christopher M. Hindson, Erin R. Chia, Amy L. Hiddessen, Benjamin J. Hindson, Chunxiao Han, Yaqi Wang
Abstract: Compositions and methods for the isolation of protein-nucleic acid complexes and microvesicles (collectively referred to as “bioparticles”) released by mammalian cells into body fluids or cell culture media are provided. Isolated bioparticles of the invention contain biological molecules that are useful as diagnostic/prognostic biomarkers or for identification of therapeutic targets (e.g., disease or disorder-associated miRNAs). The isolation of biological molecules as described herein results in purification and concentration of the molecules. Methods for producing bio fluids that are free of detectable bioparticles, that are largely depleted of bioparticles, or that possess a reduced concentration of bioparticles compared to a bio fluid starting material (collectively termed “bioparticle-depleted”) are also provided. Isolation of bioparticle-depleted biofluid is useful, e.g.
Type:
Grant
Filed:
August 5, 2015
Date of Patent:
June 2, 2020
Assignee:
YMIR GENOMICS, LLC
Inventors:
Patrick Shannon Pendergrast, Robert Scott Pendergrast, John Stephen Pendergrast, Anna Irmina Markowska
Abstract: The present disclosure relates to compounds comprising a negatively-charged polymer moiety which is capable of entering a nanopore and upon entering a nanopore in the presence of positive ions results in an increased flow of the positive ions through the nanopore. The present disclosure provides methods of preparing the compounds and for their use as nanopore-detectable tags, in particular, for nanopore-based nucleic acid detection and sequencing.
Type:
Grant
Filed:
August 23, 2017
Date of Patent:
June 2, 2020
Assignee:
Roche Sequencing Solutions, Inc.
Inventors:
Dmitriy Gremyachinskiy, Peter Crisalli, Andrew Trans, Ashwini Bhat
Abstract: Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.
Type:
Grant
Filed:
November 21, 2016
Date of Patent:
June 2, 2020
Assignee:
Pacific Biosciences of California, Inc.
Inventors:
Lubomir Sebo, Honey Osuna, Stephen Yue, Yuri Lapin
Abstract: Methods and devices are provided herein for surfaces for de novo nucleic acid synthesis which provide for low error rates. In addition, methods and devices are provided herein for increased nucleic acid mass yield resulting from de novo nucleic acid synthesis.
Type:
Grant
Filed:
February 3, 2016
Date of Patent:
June 2, 2020
Assignee:
TWIST BIOSCIENCE CORPORATION
Inventors:
Pierre F. Indermuhle, Eugene P. Marsh, Andres Fernandez, William Banyai, Bill J. Peck
Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.
Type:
Grant
Filed:
March 27, 2015
Date of Patent:
June 2, 2020
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki
Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.
Type:
Grant
Filed:
March 8, 2018
Date of Patent:
June 2, 2020
Assignee:
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
Inventors:
Jingyue Ju, Zengmin Li, John Robert Edwards, Yasuhiro Itagaki
Abstract: The present invention relates to a method for synthesising templated molecules. In one aspect of the invention, the templated molecules are linked to the template which templated the synthesis thereof. The invention allows the generation of libraries which can be screened for e.g. therapeutic activity.
Type:
Grant
Filed:
October 13, 2016
Date of Patent:
June 2, 2020
Assignee:
NUEVOLUTION A/S
Inventors:
Henrik Pedersen, Alex Haahr Gouliaev, Thomas Franch, Christian Klarner Sams, Eva Kampmann Olsen, Frank Abilgaard Slok, Gitte Nystrup Husemoen, Jakob Felding, Lene Hyldtoft, Mads Norregaard-Madsen, Michael Anders Godskesen, Sanne Schroder Glad, Thomas Thisted, Per-Ola Freskgard, Anette Holtmann