Patents Examined by Kagnew H. Gebreyesus
  • Engineered phenylalanine ammonia lyase polypeptides
    Patent number: 10358639
    Abstract: The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
    Type: Grant
    Filed: October 22, 2018
    Date of Patent: July 23, 2019
    Assignee: Codexis, Inc.
    Inventors: Gjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
  • Deimmunized lysostaphin and methods of use
    Patent number: 10358636
    Abstract: Compositions comprising deimmunized lysostaphin and methods of using the same, e.g., to treat microbial infection in or on a subject, are provided.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: July 23, 2019
    Assignees: STEALTH BIOLOGICS, LLC, TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Karl E. Griswold, Chris Bailey-Kellogg, Yoonjoo Choi, Kristina Blazanovic, Hongliang Zhao, Deeptak Verma
  • Simultaneous measurement of halide ion concentration and pH
    Patent number: 10352939
    Abstract: Embodiments of the invention relate to the quantitative measurement of halide ion concentration and pH using novel fluorescent polypeptides. One embodiment of the invention provides a polypeptide containing at least one amino acid sequence selected from a group consisting of: Sequence ID No. 3, Sequence ID No. 4, Sequence ID No. 5, and Sequence ID No. 6.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: July 16, 2019
    Inventor: David Aphkhazava
  • Compositions and methods for recombinant biosynthesis of propane
    Patent number: 10337032
    Abstract: Provided are genetically engineered microorganism that catalyze the synthesis of propane and/or butanol from a suitable substrate such as glucose. Also provided are methods of engineering said genetically engineered microorganism and methods of producing propane and/or butanol using the genetically engineered microorganism.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: July 2, 2019
    Assignee: THE UNIVERSITY OF MANCHESTER
    Inventors: Nigel Scrutton, Patrik Jones, Navya Menon
  • Efficient process for producing saccharides and ethanol from a biomass feedstock
    Patent number: 10329593
    Abstract: Described herein is a process for producing saccharides and ethanol from biomass feedstock that includes (a) producing an enzyme composition by culturing a fungal strain(s) in the presence of a lignocellulosic medium, (b) using the enzyme composition to saccharify the biomass feedstock, and (c) fermenting the saccharified biomass feedstock to produce ethanol. The process is scalable and, in certain aspects, is capable of being deployed on farms, thereby allowing local production of saccharides and ethanol and resulting in a reduction of energy and other costs for farm operators. Optional steps to improve the biomass-to-fuel conversion efficiency are also contemplated, as are uses for byproducts of the process described herein.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: June 25, 2019
    Assignee: Auburn University
    Inventors: Benedict C. Okeke, Ananda K. Nanjundaswamy
  • Thermostable reverse transcriptase
    Patent number: 10323243
    Abstract: Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: June 18, 2019
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Andrew Ellington, Jared Ellefson, Jimmy Gollihar
  • Serine protease molecules and therapies
    Patent number: 10323239
    Abstract: Cell-targeted serine protease constructs are provided. Such constructs can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity. Methods and compositions for treating lapatinib or trastuzumab-resistant cancers are also provided.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: June 18, 2019
    Assignee: Research Development Foundation
    Inventors: Michael G. Rosenblum, Khalid Amanali Mohamedali, Lawrence H. Cheung
  • Lysozyme having improved enzymatic activity
    Patent number: 10323237
    Abstract: A lysozyme having improved enzymatic activity is disclosed. The amino acid sequence of the lysozyme is a modified amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence with at least 80% sequence identity of SEQ ID NO: 2, wherein the modification is a substitution of histidine at position 166 or a corresponding position with lysine.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: June 18, 2019
    Assignee: CHENGDU BEACON BIO-TECHNOLOGY CO., LTD.
    Inventors: Chun-Chi Chen, Tao Wang, Jian-Wen Huang, Rey-Ting Guo
  • Polypeptide having protease activity and methods for increasing its activity thereof
    Patent number: 10316310
    Abstract: The present invention relates to a polypeptide having protease activity comprising a zinc finger protease domain, a helix-turn-helix domain and a GAF domain. The core protein sequence of the protease is shown as SEQ ID NO: 1. The invention also relates to optimized reaction conditions for the protease and methods of increasing the protease activity.
    Type: Grant
    Filed: December 13, 2016
    Date of Patent: June 11, 2019
    Assignee: Zhejiang University
    Inventors: Yuejin Hua, Yunguang Wang, Liangyan Wang
  • Carbohydrate degrading polypeptide and uses thereof
    Patent number: 10316305
    Abstract: The invention relates to a polypeptide having hemicellulase activity. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: June 11, 2019
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Maaike Appeldoorn
  • Protein with dextran-saccharase activity, and uses
    Patent number: 10316300
    Abstract: The invention relates to: —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an amino acid sequence; —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; —a complex containing a substrate and a protein with dextran-saccharase activity —a method for synthesizing dextrans; and —dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.
    Type: Grant
    Filed: July 21, 2015
    Date of Patent: June 11, 2019
    Assignees: INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PAUL SABATIER TOULOUSE III
    Inventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Pierre Monsan, Etienne Severac, Catherine Fontagne-Faucher
  • Microorganism for expressing a human membrane protein
    Patent number: 10308971
    Abstract: The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: June 4, 2019
    Assignee: Novozymes A/S
    Inventors: Michael Schilling, Christine Lang, Andreas Raab
  • Process for enhanced production of recombinant human serum albumin in through chaperone assistance
    Patent number: 10301373
    Abstract: The present disclosure provides a process for improved production and extraction of recombinant human serum albumin (rHSA) using E. coli as a host system. The process of the instant disclosure provides enhanced recovery of soluble and functional rHSA over conventional known methods.
    Type: Grant
    Filed: May 5, 2017
    Date of Patent: May 28, 2019
    Assignee: Indian Institute of Technology, Delhi
    Inventors: Tapan Kumar Chaudhuri, Ashima Sharma
  • Engineered phenylalanine ammonia lyase polypeptides
    Patent number: 10294468
    Abstract: The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: May 21, 2019
    Assignee: Codexis, Inc.
    Inventors: Gjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
  • Mutant endoglycoceramidases with enhanced synthetic activity
    Patent number: 10294504
    Abstract: The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.
    Type: Grant
    Filed: June 29, 2015
    Date of Patent: May 21, 2019
    Assignees: La Jolla Pharmaceutical Company, The University of British Columbia
    Inventors: Karl F. Johnson, Shawn Defrees, Stephen Withers, Mark Vaughan
  • DHAD variants and methods of screening
    Patent number: 10287566
    Abstract: Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.
    Type: Grant
    Filed: January 16, 2017
    Date of Patent: May 14, 2019
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Kristen J. Kelly, Rick W. Ye
  • Mutant enzyme and production method for terpenoid using said mutant enzyme
    Patent number: 10280406
    Abstract: The object of the present invention is to efficiently produce useful terpenoid compounds, and specifically, to provide a method for preparing squalene, which is an important intermediate of terpenoid. The object can be solved by a hydroxymethylglutaryl CoA reductase (HMGR) comprising: (a) an amino acid other than alanine (A) at the 10th position in an S?2 amino acid sequence of HMGR, (b) an amino acid other than proline (P) at the 1st position from the carboxyl terminal in the DKK region of the HMG-CoA binding site of HMGR, (c) an amino acid other than alanine (A) at the 1st position in an L?2 amino acid sequence of HMGR, and (d) an amino acid other than glutamic acid (E) at the 6th position in an L?2 amino acid sequence of HMGR of the present invention.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: May 7, 2019
    Assignee: ADEKA CORPORATION
    Inventors: Toshihiko Takehana, Seiji Koike, Tomohisa Kuzuyama
  • Enzyme compositions and uses thereof
    Patent number: 10273515
    Abstract: The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.
    Type: Grant
    Filed: June 4, 2015
    Date of Patent: April 30, 2019
    Assignee: Novozymes A/S
    Inventors: Jeffrey Shasky, Debbie Yaver, Donna Moyer
  • Compositions for inducing antigen-specific tolerance
    Patent number: 10265415
    Abstract: Peptides that specifically bind erythrocytes are described. These are provided as peptidic ligands having sequences that specifically bind, or as antibodies or fragments thereof that provide specific binding, to erythrocytes. The peptides may be prepared as molecular fusions with therapeutic agents, tolerizing antigens, or targeting peptides. Immunotolerance may be created by use of the fusions and choice of an antigen on a substance for which tolerance is desired.
    Type: Grant
    Filed: February 23, 2018
    Date of Patent: April 23, 2019
    Assignee: École Polytechnique Fédérale de Lausanne (EPFL)
    Inventors: Jeffrey A. Hubbell, Stephan Kontos, Karen Y. Dane
  • Glucosyltransferase enzymes for production of glucan polymers
    Patent number: 10260053
    Abstract: Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DPw) of at least 1000. This glucan polymer comprises at least 30% alpha-1,3 linkages and at least 30% alpha-1,6 linkages. Further disclosed are glucosyltransferase enzymes that synthesize poly alpha-1,3-1,6-glucan. Ether derivatives of poly alpha-1,3-1,6-glucan and methods of using such derivatives as viscosity modifiers are also disclosed.
    Type: Grant
    Filed: March 23, 2018
    Date of Patent: April 16, 2019
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Jayme L. Paullin, Mark S. Payne, T. Joseph Dennes, Yefim Brun, Rakesh Nambiar, Thomas H Scholz