Patents Examined by Kagnew H. Gebreyesus
  • Delivery of negatively charged proteins using cationic lipids
    Patent number: 10912833
    Abstract: Compositions, methods, strategies, kits, and systems for the delivery of negatively charged proteins, protein complexes, and fusion proteins, using cationic polymers or lipids are provided. Delivery of proteins into cells can be effected in vivo, ex vivo, or in vitro. Proteins that can be delivered using the compositions, methods, strategies, kits, and systems provided herein include, without limitation, enzymes, transcription factors, genome editing proteins, Cas9 proteins, TALEs, TALENs, nucleases, binding proteins (e.g., ligands, receptors, antibodies, antibody fragments; nucleic acid binding proteins, etc.), structural proteins, and therapeutic proteins (e.g., tumor suppressor proteins, therapeutic enzymes, growth factors, growth factor receptors, transcription factors, proteases, etc.), as well as variants and fusions of such proteins.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: February 9, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: David R. Liu, David B. Thompson, John Anthony Zuris
  • Allosteric split trehalase biosensor
    Patent number: 10900960
    Abstract: The present disclosure relates to a method referred to herein as the “split trehalase assay biosensor” (also referred to herein as “STIGA”) is based on the use of engineered E. coli trehalase to detect analytes such as antibodies in a sample. The trehalase is engineered in a way such that the enzyme is split into two inactive fragments (N-terminal fragment H and C-terminal fragment A) with antigens fused to both fragments. When bivalent antibodies react specifically with the fused antigens, two inactive trehalase fragments are brought in close proximity to restore the activity of trehalase. The restored trehalase will hydrolyze trehalose into two glucose molecules which can be measured using existing glucose detection methods such as glucometer, Benedict's reagent, or ACCU-CHEK AVIVA® glucose test strips.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: January 26, 2021
    Assignee: Creative Protein Solutions Inc.
    Inventor: Jeroen De Buck
  • Compositions and methods for using genetically modified enzymes
    Patent number: 10894952
    Abstract: The disclosure relates to the biosynthesis of cannabinoids and related prenylated phenolic compounds using recombinant enzymes. In particular, the disclosure provides recombinant mutant ORF2 enzymes engineered to produce a greater amount of a desired product, or to have a greater ability to catalyze a reaction using a desired substrate, as compared to WT ORF2. The disclosure also provides methods of preparing such ORF2 mutant enzymes; as well as methods of use thereof in improving the biosynthesis of cannabinoids and related prenylated phenolic compounds.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: January 19, 2021
    Assignee: RENEW BIOPHARMA, INC.
    Inventors: Michael Mendez, Joseph Noel, Michael Burkart, Jeremy Lanoiselee, Kyle Botsch, Matthew Saunders
  • Production method for rubber particles having membrane-binding protein bound thereto, production method for pneumatic tire, and production method for rubber product
    Patent number: 10889623
    Abstract: The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: January 12, 2021
    Assignees: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Haruhiko Yamaguchi, Yukino Inoue, Kazuhisa Fushihara, Seiji Takahashi, Satoshi Yamashita, Toru Nakayama, Yuzuru Tozawa
  • Fermentative glycerol-free ethanol production
    Patent number: 10883110
    Abstract: The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.
    Type: Grant
    Filed: June 4, 2020
    Date of Patent: January 5, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Jacobus Thomas Pronk, Antonius Jeroen Adriaan Van Maris, Victor Gabriel Guadalupe Medina
  • Compositions and methods for making selenocysteine containing polypeptides
    Patent number: 10876142
    Abstract: Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: December 29, 2020
    Assignee: YALE UNIVERSITY
    Inventors: Dieter Söll, Caroline Aldag, Michael Hohn, Takahito Mukai
  • Engineered phenylalanine ammonia lyase polypeptides
    Patent number: 10870843
    Abstract: The present invention provides engineered phenylalanine ammonia-lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia-lyase (PAL) polypeptides.
    Type: Grant
    Filed: October 15, 2019
    Date of Patent: December 22, 2020
    Assignee: Codexis, Inc.
    Inventors: Gjalt W. Huisman, Nicholas J. Agard, Benjamin Mijts, Jonathan Vroom, Xiyun Zhang
  • Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
    Patent number: 10865397
    Abstract: Disclosed herein are recombinant polynucleotide sequences, vectors, host cells and methods for producing astaxanthin. The recombinant polynucleotide sequence is designed to provide a higher level of astaxanthin precursors via a shorter metabolic pathway, and thereby attains higher level of end products (e.g., astaxanthin) with desired stereoisomeric form and/or esterified form.
    Type: Grant
    Filed: November 26, 2018
    Date of Patent: December 15, 2020
    Assignee: ACADEMIA SINICA
    Inventors: Jui-Jen Chang, Caroline Thia, Hao-Yeh Lin, Yu-Ju Lin, Chieh-Chen Huang, Wen-Hsiung Li
  • Benzylisoquinoline alkaloids (BIA) producing microbes, and methods of making and using the same
    Patent number: 10858681
    Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: December 8, 2020
    Assignee: THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
    Inventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard, Stephanie Galanie
  • Thermostable reverse transcriptase
    Patent number: 10858652
    Abstract: Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate.
    Type: Grant
    Filed: May 8, 2019
    Date of Patent: December 8, 2020
    Assignee: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Andrew Ellington, Jared Ellefson, Jimmy Gollihar
  • Method for producing metal nanoparticles and metal sulfide nanoparticles using a recombinant microorganism
    Patent number: 10851390
    Abstract: The present invention relates to a method of producing metal nanoparticles and metal sulfide nanoparticles using a recombinant microorganism co-expressing metallothionein and phytochelatin synthase, which are heavy metal-adsorbing proteins, and to the use of metal nanoparticles and metal sulfide nanoparticles synthesized by the method. The present invention provides a method for synthesizing metal nanoparticles which have been difficult to synthesize by conventional biological methods. The present invention makes it possible to synthesize metal nanoparticles in an environmentally friendly and cost-effective manner, and also makes it possible to synthesize metal sulfide nanoparticles. In addition, even metal nanoparticles which could have been produced by conventional chemical or biological methods are produced in a significantly increased yield by use of the method of the present invention.
    Type: Grant
    Filed: December 29, 2016
    Date of Patent: December 1, 2020
    Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Sang Yup Lee, Yoojin Choi
  • Engineered microorgansims and methods for improved crotyl alcohol production
    Patent number: 10844404
    Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.
    Type: Grant
    Filed: April 7, 2016
    Date of Patent: November 24, 2020
    Assignee: Genomatica Inc.
    Inventors: Stephanie J. Culler, Nicholas Eakley, Kevin Gregory Hoff, Robin E. Osterhout, Priti Pharkya, Erik C. Ralph, Stephen Van Dien
  • Generation and comparative kinetic analysis of new glycosynthase mutants from endoglycosidases for antibody glycoengineering
    Patent number: 10836815
    Abstract: The present invention provides for recombinant Endo-S mutants (named Endo-S glycosynthases) that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sugar chain is added to a fucosylated or nonfucosylated GlcNAc-IgG acceptor. As such, the present invention allows for the synthesis and remodeling of therapeutic antibodies thereby providing for certain biological activities, such as, prolonged half-life time in vivo, less immunogenicity, enhanced in vivo activity, increased targeting ability, and/or ability to deliver a therapeutic agent.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: November 17, 2020
    Assignee: UNIVERSITY OF MARYLAND, COLLEGE PARK
    Inventors: Lai-Xi Wang, Xin Tong, Tiezheng Li
  • Fermentative production of oligosaccharides
    Patent number: 10829508
    Abstract: The invention relates to a microbial production of recombinant oligosaccharides, particularly of human milk oligosaccharides (HMOs), using a genetically modified microorganism, particularly E. coli, in antibiotic-free cultivating medium.
    Type: Grant
    Filed: December 19, 2016
    Date of Patent: November 10, 2020
    Assignee: GLYCOM A/S
    Inventors: Manos Papadakis, Margit Pedersen, Katrine Bych
  • Compositions and methods for recombinant biosynthesis of propane
    Patent number: 10822624
    Abstract: Provided are genetically engineered microorganism that catalyze the synthesis of propane and/or butanol from a suitable substrate such as glucose. Also provided are methods of engineering said genetically engineered microorganism and methods of producing propane and/or butanol using the genetically engineered microorganism.
    Type: Grant
    Filed: May 7, 2019
    Date of Patent: November 3, 2020
    Assignee: C3 BIO-TECHNOLOGIES LIMITED
    Inventors: Nigel Scrutton, Patrik Jones, Navya Menon
  • Polypeptides capable of producing glucans having alpha-1,2 branches and use of the same
    Patent number: 10822383
    Abstract: Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.
    Type: Grant
    Filed: November 22, 2016
    Date of Patent: November 3, 2020
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Qiong Cheng, Robert DiCosimo, Jahnavi Chandra Prasad, Zhenghong Zhang
  • Erythrocyte-binding therapeutics
    Patent number: 10800838
    Abstract: Peptides that specifically bind erythrocytes are described. These are provided as peptidic ligands having sequences that specifically bind, or as antibodies or fragments thereof that provide specific binding, to erythrocytes. The peptides may be prepared as molecular fusions with therapeutic agents, tolerizing antigens, or targeting peptides. Immunotolerance may be created by use of the fusions and choice of an antigen on a substance for which tolerance is desired.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: October 13, 2020
    Assignee: École Polytechnique Fédérale de Lausanne (EPFL)
    Inventors: Jeffrey A. Hubbell, Stephan Kontos, Karen Y. Dane
  • Glucosyltransferase enzymes for production of glucan polymers
    Patent number: 10793838
    Abstract: Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DPw) of at least 1000. This glucan polymer comprises at least 30% alpha-1,3 linkages and at least 30% alpha-1,6 linkages. Further disclosed are glucosyltransferase enzymes that synthesize poly alpha-1,3-1,6-glucan. Ether derivatives of poly alpha-1,3-1,6-glucan and methods of using such derivatives as viscosity modifiers are also disclosed.
    Type: Grant
    Filed: April 12, 2019
    Date of Patent: October 6, 2020
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Jayme L. Paullin, Mark S. Payne, T. Joseph Dennes, Yefim Brun, Rakesh Nambiar, Thomas H. Scholz
  • Methods and compositions for treatment of Lafora disease
    Patent number: 10781433
    Abstract: In certain embodiments, the present disclosure provides compositions and methods for treating Lafora Disease.
    Type: Grant
    Filed: September 8, 2017
    Date of Patent: September 22, 2020
    Assignee: Valerion Therapeutics, LLC
    Inventor: Dustin D. Armstrong
  • Dibasic organic acid producing strain and preparation and application of same
    Patent number: 10781462
    Abstract: Provided are an engineered strain for synthesizing a dibasic organic acid and preparation and application of same. The engineered strain introduces or up-regulates expression of a positive regulator gene for synthesis of a dibasic organic acid, and/or down-regulates expression of a negative regulator gene for synthesis of a dibasic organic acid, as compared with the origin strain of the engineered strain, the producing capability for producing the dibasic organic acid is improved.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: September 22, 2020
    Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Chaoguang Tian, Jingen Li, Chuannan Long, Tao Sun, Liangcai Lin, Jing Xu, Qian Liu, Jingxiao Ji, Wenliang Sun, Yanhe Ma