Patents Examined by Kara D Johnson
  • Patent number: 11660271
    Abstract: A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: May 30, 2023
    Assignee: FOREVER LABS, INC.
    Inventors: Mark Katakowski, Ann Hozeska-Solgot
  • Patent number: 11655456
    Abstract: Disclosed are methods of assessing the ability of a candidate therapeutic agent to reverse, reduce or prevent intestinal injury by a potential toxic agent using a three-dimensional, engineered, bioprinted, biological intestinal tissue model. Also disclosed are methods of assessing the effect of an agent on intestinal function, the method comprising contacting the agent with a three-dimensional, engineered, bioprinted, biological intestinal tissue model.
    Type: Grant
    Filed: November 10, 2017
    Date of Patent: May 23, 2023
    Assignee: Organovo, Inc.
    Inventors: Kelsey Nicole Retting, Deborah Lynn Greene Nguyen, Lauran Madden
  • Patent number: 11655449
    Abstract: The present invention includes a method of preparing differentiated endodermal cells comprising growing human pluripotent stem cells (hPSC) under serum free conditions for 3 days on a layer of fibronectin or a gelatinous protein layer in the presence of high activin/TGF-? with a GSK3 inhibitor and a PI3K/mTORC inhibitor in a basal differentiation medium; adding a knock out serum replacement to the media starting on day 4 through day 7; and harvesting the endodermal cells grown therein.
    Type: Grant
    Filed: June 3, 2020
    Date of Patent: May 23, 2023
    Inventors: Jeffrey Fair, William Sam Fagg
  • Patent number: 11634689
    Abstract: A cell medium for in vitro inducing chondrogenesis or tenogenesis in mesenchymal stem cells (MSCs). The medium is a glucose medium supplemented with at least one growth factor is chosen from the group of fibroblast growth factors (FGF) or the group of transforming growth factors (TGF), and the FGF or TGF is present in a total concentration of between 1 and 15 ng/ml. In both cases, IGF can be added to enhance the induction process. The use of the cell medium, a method for inducing isolated mesenchymal stem cells (MSCs) and a cell composition obtained by the method are also provided.
    Type: Grant
    Filed: November 20, 2019
    Date of Patent: April 25, 2023
    Inventors: Sarah Yolande Kristel Broeckx, Jan Hilda Marie Jozef Spaas
  • Patent number: 11617817
    Abstract: The present disclosure provides patterned biomaterials having organized cords and extracellular matrix embedded in a 3D scaffold. According, the present disclosure provides compositions and applications for patterned biomaterials. Pre-patterning of these biomaterials can lead to enhanced integration of these materials into host organisms, providing a strategy for enhancing the viability of engineered tissues by promoting vascularization.
    Type: Grant
    Filed: October 1, 2019
    Date of Patent: April 4, 2023
    Assignees: The Trustees of the University of Pennsylvania, Massachusetts Institute of Technology
    Inventors: Christopher S. Chen, Jan D. Baranski, Ritika Chaturvedi, Michael T. Yang, Kelly Stevens, Sangeeta Bhatia
  • Patent number: 11617768
    Abstract: The present invention relates to methods for obtaining skeletal muscle derived cells (SMDC), and the use of SMDCs in a method of preventing and/or treating neuromyopathies and/or myopathies, wherein the neuromyopathy and/or myopathy is incontinence, in particular a urinary and/or an anal or fecal incontinence.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: April 4, 2023
    Assignee: INNOVACELL AG
    Inventors: Marco Thurner, Eva Margreiter, Wolfgang Schwaiger, Faheem Muhammad Asim, Rainer Marksteiner
  • Patent number: 11613729
    Abstract: Improved hybrid neurovascular spheroids and methods for making the same are provided. In some embodiments of a method for making a hybrid neurovascular spheroid, the method includes i) propagating cortical cells to form a cortical spheroid; ii) propagating endothelial cells to form an endothelial spheroid; iii) propagating mesenchymal stem cells to form a mesenchymal cell culture; and iv) combining the cortical spheroid, endothelial spheroid, and mesenchymal spheroid under conditions to form the hybrid neurovascular spheroid.
    Type: Grant
    Filed: March 17, 2020
    Date of Patent: March 28, 2023
    Assignee: The Florida State University Research Foundation, Inc.
    Inventors: Yan Li, Teng Ma
  • Patent number: 11606953
    Abstract: A viable disc regenerative composition has a micronized material of nucleus pulposus and a biological composition made from a mixture of mechanically selected allogeneic biologic material derived from bone marrow having non-whole cellular components including vesicular components and active and inactive components of biological activity, cell fragments, cellular excretions, cellular derivatives, and extracellular components; and wherein the mixture is compatible with biologic function and further includes non-expanded whole cells. The biological composition is predisposed to demonstrate or support elaboration of active volume or spatial geometry consistent in morphology with that of disc tissue. The viable disc regenerative composition extends regenerative resonance that compliments or mimics disc tissue complexity.
    Type: Grant
    Filed: October 15, 2020
    Date of Patent: March 21, 2023
    Assignee: Vivex Biologies Group, Inc.
    Inventors: Harry Thomas Temple, Timothy Ganey, Stephanie Gonzalez, Tracy Scott Anderson, Shabnam Namin
  • Patent number: 11608486
    Abstract: Embodiments for loading and expanding particular cell types are described. Embodiments may include the use of hollow fiber membranes with particular characteristic such as hollow fibers with inner diameters that provide mechanical stimulus (e.g., radius of curvature greater than a dimension of a cell). In addition, embodiments may provide for manipulation of flow rates and other features that also provide mechanical stimuli and promote or enhance the growth of particular types of cells.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: March 21, 2023
    Assignee: Terumo BCT, Inc.
    Inventors: Mark E. Jones, Brian J. Nankervis, Kristina E. Fuerst, Jon A. Dodd
  • Patent number: 11597911
    Abstract: This invention relates, inter alia, to compositions of low serum or serum free media and methods for the expansion of T cell populations and methods for using such populations of cells. In some aspects, the invention relates to compositions and methods for the selective expansion of T cell subpopulations.
    Type: Grant
    Filed: February 26, 2018
    Date of Patent: March 7, 2023
    Assignees: Life Technologies Corporation, The Trustees of the University of Pennsylvania
    Inventors: Angel M. Varela-Rohena, Melanie B. Andolina, James L. Riley, Andrew Medvec
  • Patent number: 11591571
    Abstract: The invention relates to a method for detecting residual, undifferentiated pluripotent stem cells (PSCs) in a culture of cells differentiated from PSCs, the method comprising: culturing the cells on a substrate coated with laminin-521 and E-cadherin in a medium comprising a ROCK inhibitor; quantitating in the cultured cells expression of a marker of residual, undifferentiated PSCs; and comparing the marker expression in the cultured cells with the marker expression in a reference culture of cells comprising a known proportion of PSCs, wherein lower marker expression in the culture of cells than marker expression in the reference culture of cells indicates absence of residual, undifferentiated PSCs in the cultured cells or presence of residual, undifferentiated PSCs in the cultured cells at a proportion lower than the known proportion of PSCs in the reference culture of cells.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: February 28, 2023
    Inventors: Igor Slukvin, Derek Hei, Diana Drier
  • Patent number: 11566233
    Abstract: The invention relates to a three-dimensional in vitro alveolar lung model comprising essentially the four cells types as follows: alveolar type II epithelial cells able to secrete (lung laying) surfactant, endothelial cells which forms the inner lining of capillaries providing a permeable barrier, dendritic-like cells, such as non-differentiated THP-1, linking innate and adaptive immunity and macrophage-like cells, able to participate to defense mechanisms by ingesting foreign materials by phagocytosis. The invention also relates to a process for preparing said model, and its use for assessing the irritation potential or toxicity of inhalable products such as particles or molecules on the alveolar barrier of lungs, and also for determining and/or predicting the sensitizing effects of inhalable products such as particles or molecules on the alveolar barrier of lungs.
    Type: Grant
    Filed: December 26, 2017
    Date of Patent: January 31, 2023
    Inventors: Aline Chary, Arno Gutleb, Tommaso Serchi, Brunhilde Blomeke
  • Patent number: 11566232
    Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.
    Type: Grant
    Filed: September 6, 2018
    Date of Patent: January 31, 2023
    Inventors: Maiko Tanabe, Shizu Takeda, Masahiro Okanojo, Hiroko Hanzawa, Masao Washizu, Kennedy Omondi Okeyo
  • Patent number: 11566229
    Abstract: Methods for derivation, culture, and maturation of small hepatic progenitor cells are described.
    Type: Grant
    Filed: August 30, 2019
    Date of Patent: January 31, 2023
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: James A. Thomson, Srikumar Sengupta
  • Patent number: 11542475
    Abstract: The present invention relates generally to a three-dimensional cell and tissue culture system for the female reproductive tract. In particular provided herein the system includes individual female reproductive cultures in a dynamic microfluidic setting or integrated using a microfluidic microphysiologic system. In some embodiments, the present invention provides ex-vivo female reproductive tract integration in a three dimensional (3D) microphysiologic system.
    Type: Grant
    Filed: October 21, 2019
    Date of Patent: January 3, 2023
    Assignees: The Board of Trustees of the University of Illinois, Northwestern University
    Inventors: Teresa K. Woodruff, Ji-Yong Julie Kim, Joanna E. Burdette, Spiro Getsios, Sevim Yildiz Arslan, Shuo Xiao, Jie Zhu
  • Patent number: 11534529
    Abstract: Methods of producing and methods of treatment using a nipple areolar complex extracellular matrix. A method for processing tissue comprises harvesting a human nipple areolar complex tissue, treating the tissue with a stabilizing medium, wherein the medium stabilizes the tissue during transport, decellularizing the tissue, and sterilizing the tissue to form a human nipple areolar complex extracellular matrix scaffold (ECMS). A method of treating an individual with a need for a nipple reconstruction comprises forming an ECMS and applying the ECMS to the individual for nipple areolar regeneration.
    Type: Grant
    Filed: August 3, 2020
    Date of Patent: December 27, 2022
    Assignee: NOVOTHELIUM, LLC
    Inventors: Bianca G. Cerqueira, Lauren E. Cornell
  • Patent number: 11530387
    Abstract: The present invention provides a method for producing a unilocular adipocyte including inducing differentiation into unilocular adipocytes of mesenchymal cells having differentiation potency into adipocytes by culturing the mesenchymal cells in suspension in a liquid medium composition capable of culturing cells or tissues in suspension, wherein the liquid medium composition contains a polymer compound having an anionic functional group that binds via a divalent metal cation to form a structure capable of suspending cells or tissues, and the method wherein the polymer compound is polysaccharide, preferably polysaccharide containing a glucuronic acid moiety, more preferably deacylated gellan gum, diutan gum or xanthan gum or a salt thereof.
    Type: Grant
    Filed: November 16, 2018
    Date of Patent: December 20, 2022
    Inventor: Tatsuro Kanaki
  • Patent number: 11505784
    Abstract: The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: November 22, 2022
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Christoph Clemens, Jochen Schaub, Marie Link, Peter Schorn, Torsten Schulz
  • Patent number: 11492598
    Abstract: The present disclosure provides a substrate for cell culture. Systems comprising the substrate, and methods for using and manufacturing the substrate are also disclosed herein.
    Type: Grant
    Filed: December 30, 2021
    Date of Patent: November 8, 2022
    Inventor: Ying-Chih Chang
  • Patent number: 11485955
    Abstract: The present invention discloses formulation of a serum-free medium used for human pluripotent stem cells, which comprises the following raw materials: inorganic salt components, organic components, amino acids and amino acid salts, energy substances and metabolic intermediates, vitamins and antioxidants, proteins and polypeptides, trace elements and chromogenic substances; while the culture process comprises the following steps: selecting a basic formulation, performing combination screening, identifying and evaluating results, and testing a new formulation of culture; and proportioning according to the following methods: adding aforesaid raw materials into 950 ml of water for injection, stirring gently until dissolved, and finally adding 2.438 g of sodium bicarbonate, and stirring gently until dissolved, and then adding 1 liter of water for injection, adjusting the pH to the desired value with 1 mol/L sodium hydroxide solution or 1 mol/L hydrochloric acid solution, finally filtering sterilized with 0.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: November 1, 2022
    Inventor: Tao Yang