Abstract: Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation.
Type:
Grant
Filed:
July 6, 2018
Date of Patent:
March 23, 2021
Assignees:
The Brigham and Women's Hospital, Inc., Massachusetts Institute of Technology
Inventors:
Jeffrey Michael Karp, Xiaolei Yin, Marc David Succi, Robert Samuel Langer
Abstract: Methods of inducing a polarization of macrophages. The method includes obtaining a blood fraction, fractionating the blood fraction to produce a blood fraction, and contacting the blood fraction with a source of macrophages. A blood fraction including platelet-poor plasma polarizes the source of macrophages into M1 macrophages. A blood faction including a protein solution polarizes the source of macrophages into M2 macrophages.
Type:
Grant
Filed:
October 30, 2017
Date of Patent:
March 16, 2021
Assignee:
Biomet Biologics, LLC
Inventors:
Jennifer E. Woodell-May, Joel C. Higgins, William King
Abstract: The present invention provides methods for the sequential and temporally-regulated administration of pancreatic transcription factors to induce non-pancreatic cells to transdifferentiate and mature along the pancreatic ?-cell lineage. The present invention also provides methods for identifying, isolating and enriching transdifferentiation predisposed cells and methods for treating a degenerative pancreatic disorder such as diabetes.
Type:
Grant
Filed:
April 25, 2018
Date of Patent:
March 16, 2021
Assignees:
ORGENESIS LTD., TEL HASHOMER MEDICAL RESEARCH INFRASTRUCTURE AND SERVICES LTD.
Abstract: The present application relates to use of transglutaminases to treat various tissue matrix products. The methods can include application of a transglutaminase to a partially denatured collagen-containing tissue matrix and implantation of the tissue matrix. The transglutaminase can facilitate adhesion with another collagen-containing tissue matrix, tissue surrounding the tissue matrix after implantation, or both.
Type:
Grant
Filed:
January 29, 2018
Date of Patent:
March 9, 2021
Assignee:
LifeCell Corporation
Inventors:
Eric Stec, Ming F. Pomerleau, Rick T. Owens, Richard Seeman
Abstract: Provided herein is technology relating to immunoisolation of cells and tissues, including, but not exclusively, to compositions, methods, and kits for encapsulating cells and/or tissues within an immunoisolating device to protect the cells/or tissues from host immune rejection.
Type:
Grant
Filed:
August 25, 2016
Date of Patent:
February 16, 2021
Assignee:
THE REGENTS OF THE UNIVERSITY OF MICHIGAN
Abstract: The present invention relates to directed differentiation and maturation of mammalian hepatocytes, such as human hepatocytes. The hepatocyte obtained in accordance with the present invention show a phenotype which is more similar to that of primary hepatocytes than previously shown. In particular, the present invention relates to exposure of mammalian hepatocytes, such as human hepatocytes, to at least one maturation factor selected from the group consisting of Src kinase inhibitors, vitamin D including precursors, metabolites and analogs thereof, hypoxia inducing compounds, sphingosine and sphingosine derivatives, activators of peroxisome proliferator-activated receptors (PPARs), platelet-activating factor (PAF), PKC inhibitors, and combinations thereof.
Abstract: Systems, techniques and methods for estimating the metabolic state or flux, e.g., the body energy state (“BES”) of a patient, are disclosed. The BES provides a deep insight into the nutritional needs of the patient, thus allowing for a sort of exquisite glycemic control with regard to the patient. The invention discloses systems and methods for estimating fractional gluconeogenesis. The invention also discloses systems and methods for estimating and targeting patient blood lactate concentration, both as a target itself and as an intermediate step to estimating and targeting patient fractional gluconeogenesis glucose production. Nutritional support methods and formulations are also disclosed. The invention is suitable for any sort of patient, including those who are injured, such as with traumatic brain injury, ill, or have other conditions that stress the metabolic system.
Abstract: The present disclosure relates to methods for performing assays for active migration and cytotoxicity of a therapeutic agent towards tumor cells, e.g., immune cell and/or drug homing, migration, and tumor cytotoxicity. The methods are performed in labware that provide opportunities for a therapeutic agent, such as an immune cell or a drug, to migrate toward tumor cells, including tumor cells growing in a 3D spheroid conformation. The methods allow for, among other uses, the investigation of the effects of a therapeutic agent, such as immune cells or a drug, on tumor cells, and enable the investigation of homing, tumor cytotoxicity, and tumor immune evasion in a single, easy-to-use, high throughput system for more in vivo-like testing.
Abstract: The present invention is related to a medium additive composition comprising peroxidasin for serum-free cell culture, and a method of performing serum-free culture of cells by using the same.
Type:
Grant
Filed:
January 12, 2017
Date of Patent:
November 17, 2020
Assignee:
THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Abstract: Uses of expanded cord blood hematopoietic stem/progenitor cells (HSPC) are described. Examples include to reduce transplant rejection, to induce immune tolerance, to reduce total parenteral nutrition (TPN) feeding, opioid use, mucositis, and hospitalization following a medical procedure and to reduce graft versus host disease (GVHD) following an allogeneic transplant.
Abstract: The present invention relates to a dry composition, which upon addition of an aqueous medium forms a substantially homogenous paste suitable for use in haemostasis procedures. The paste forms spontaneously upon addition of the liquid, hence no mechanical mixing is required for said paste to form. The invention further relates to methods of preparing said dry composition, a paste made from said dry composition and use of said paste for medical and surgical purposes.
Abstract: The current invention concerns a cell medium for in vitro inducing chondrogenesis or tenogenesis in mesenchymal stem cells (MSCs). The medium includes a glucose medium supplemented with at least one growth factor. The growth factor is selected from fibroblast growth factors (FGF) or transforming growth factors (TGF). FGF or TGF is present in a total concentration of between 1 and 15 ng/ml. In both cases, IGF can be added to enhance the induction process. In a further aspect, the invention provides for a use of such cell medium as well as a method for inducing isolated mesenchymal stem cells (MSCs) and a cell composition obtained by such method.
Type:
Grant
Filed:
December 6, 2013
Date of Patent:
October 13, 2020
Assignee:
Global Stem Cell Technology
Inventors:
Sarah Yolande Kristel Broeckx, Jan Hilda Marie Jozef Spaas
Abstract: Methods for treating tissue matrices and tissue matrices produced according to the methods are provided. The methods can include treating select portions of a tissue matrix with a fluid containing at least one agent to produce a tissue matrix with variable mechanical and/or biological properties.
Type:
Grant
Filed:
May 26, 2017
Date of Patent:
October 6, 2020
Assignee:
LIFECELL CORPORATION
Inventors:
Ming F. Pomerleau, Hua Wan, Hui Xu, Dennis Y. Lee
Abstract: A method for expanding circulating tumor cell in vitro includes preparing a cell culture tool having a multi-particle colloidal crystal layer, preparing a cell solution including circulating tumor cells, and contacting the cell solution with the multi-particle colloidal crystal layer, to attach the circulating tumor cells in the cell solution to the multi-particle colloidal crystal layer and rapidly expand by 20 times or more. The multi-particle colloidal crystal layer at least includes first particles having a particle size of 1000 to 5000 nm and second particles having a particle size of 20 to 400 nm. The culture medium in the cell solution at least includes a platelet lysate.
Abstract: Provided herein is a three-dimensional co-culture of adipocytes and macrophages, wherein, in a hydrogel scaffold containing adipocytes and macrophages, the adipocytes and the macrophages are co-cultured to form a fat-like tissue, which can be then utilized in the studies and medicine development for treating metastatic diseases associated with adipose tissue.
Type:
Grant
Filed:
July 22, 2016
Date of Patent:
September 1, 2020
Assignee:
Korea Research Institute of Chemical Technology
Inventors:
Ki Young Kim, Sung Bum Park, Sang Dal Rhee, Hi Youn Kim, Won Hoon Jung
Abstract: The present invention relates to the field of implants for the formation/regeneration of lymph nodes. In particular, the present invention relates to an implant comprising a biodegradable scaffold and lymph node fragments immobilized therein and/or thereon, to a method of manufacturing such an implant and to uses of such an implant.
Type:
Grant
Filed:
December 18, 2015
Date of Patent:
August 11, 2020
Assignee:
Klinikum rechts der Isar der Technischen Universität München
Inventors:
Min-Seok Kwak, Jan-Thorsten Schantz, Dietmar Werner Hutmacher, Mohit Prashant Chhaya, Elizabeth Rosado Balmayor
Abstract: Methods of producing and methods of treatment using a nipple areolar complex extracellular matrix. A method for processing tissue comprises harvesting a human nipple areolar complex tissue, treating the tissue with a stabilizing medium, wherein the medium stabilizes the tissue during transport, decellularizing the tissue, and sterilizing the tissue to form a human nipple areolar complex extracellular matrix scaffold (ECMS). A method of treating an individual with a need for a nipple reconstruction comprises forming an ECMS and applying the ECMS to the individual for nipple areolar regeneration.
Abstract: A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.
Type:
Grant
Filed:
May 4, 2016
Date of Patent:
July 7, 2020
Assignee:
PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Inventors:
Jennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
Abstract: A microfabricated platform for mimicking the liver zonation and an evaluating method of zone-specific hepatotoxicity using the same is provided. The microfabricated platform for mimicking the liver zonation prepared according to the method of the present invention is divided into three zones of zone 1, zone 2 and zone 3 similarly to in vivo liver tissue and thus the zone-specific hepatotoxicity of a drug in the liver can be evaluated using the same. According to the present invention, the zone-specific hepatotoxicity results can be analyzed quantitatively by using image analysis, so that the platform of the present invention can be effectively used for in vitro screening of zone-specific hepatotoxicity.
Type:
Grant
Filed:
March 5, 2019
Date of Patent:
June 16, 2020
Assignee:
KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY
Inventors:
Jung-Hwa Oh, Jaehwan Ahn, Seokjoo Yoon, Heeyoung Yang, Jun-Ho Ahn, Mi-Sun Choi, Soojin Kim, Se-Myo Park, Hyoung-Yun Han
Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing ? cell maturation, and isolated populations of SC-? cells for use in various applications, such as cell therapy.
Type:
Grant
Filed:
July 23, 2018
Date of Patent:
May 19, 2020
Assignee:
President and Fellows of Harvard College
Inventors:
Quinn P. Peterson, Felicia J. Pagliuca, Douglas A. Melton, Jeffrey R. Millman, Michael Saris Segel, Mads Gurtler