Patents Examined by Karen Cochrane Carlson
  • Alkaline stable Fc-binding proteins for affinity chromatography
    Patent number: 11014967
    Abstract: The present invention relates to Fc binding proteins comprising one or more Fc binding domains wherein at least one domain comprises of an amino acid sequence selected from the group consisting of SEQ ID NOs: 1-6 or 21. The invention further relates to affinity matrices comprising the Fc binding proteins of the invention. The invention also relates to a use of these Fc binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Fc binding proteins of the invention.
    Type: Grant
    Filed: August 7, 2017
    Date of Patent: May 25, 2021
    Assignee: Repligen Corporation
    Inventors: Paul Knick, Erik Fiedler, Ulrich Haupts, Maren Meysing
  • Variant Cas9 polypeptides comprising internal insertions
    Patent number: 11008555
    Abstract: The present disclosure provides variant Cas9 polypeptides, where a variant Cas9 polypeptide of the present disclosure comprises an internal insertion of a heterologous polypeptide. The present disclosure provides nucleic acids comprising nucleotide sequences encoding the variant Cas9 polypeptides. The present disclosure provides host cells comprising a variant Cas9 polypeptide of the present disclosure, or comprising a nucleic acid encoding a variant Cas9 polypeptide of the present disclosure. The present disclosure provides methods of binding and/or modifying a target nucleic acid, involving use of a variant Cas9 polypeptide of the present disclosure.
    Type: Grant
    Filed: September 15, 2016
    Date of Patent: May 18, 2021
    Assignee: The Regents of the University of California
    Inventors: Benjamin Oakes, David Savage, Dana Nadler, Abraham I. Flamholz, Jennifer A. Doudna
  • Carbohydrate-binding protein
    Patent number: 11001824
    Abstract: The carbohydrate processing enzyme PNGase F was catalytically inactivated through mutation. Additional mutations yielded a catalytically inactive carbohydrate-binding protein with lectin-like properties including high affinity and specificity N-linked glycans, O-linked glycans, or both.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: May 11, 2021
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Kausar N. Samli, Robert J. Woods, Loretta Yang
  • Methods for producing carbo sugars and applications thereof
    Patent number: 11001848
    Abstract: Described herein are biological devices and methods for using the same to produce carbo sugars. The biological devices include microbial cells transformed with a DNA construct containing genes for producing a cellulose synthase and galactomannan galactosyltransferase. In some instances, the biological devices also include a gene for lipase. Methods for altering the viscosity of petroleum oil using the carbo sugars are also described herein. Finally, methods for degreasing or decontaminating water mixed with petroleum oil or other fatty substances or a surface coated with petroleum oil or other fatty substances using the carbo sugars are described herein.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: May 11, 2021
    Assignee: BIOCAPITAL HOLDINGS, LLC
    Inventors: Raul Cuero Rengifo, Juliana Londoño Murillo, Mariana Sanchez Londoño, Daniel Agudelo Valencia, Juan Miguel Sanchez Bustamante, Mario Antonio Franco Jimenez, Lina Clemencia Sanchez Bustamante
  • Recombinant milk proteins
    Patent number: 10988521
    Abstract: Provided herein are compositions and methods for producing recombinant milk proteins, as well as food compositions comprising the same. In aspects, the recombinant milk proteins of the disclosure are recombinant fusion proteins comprising casein and beta-lactoglobulin.
    Type: Grant
    Filed: December 18, 2020
    Date of Patent: April 27, 2021
    Assignee: Alpine Roads, Inc.
    Inventors: Viviane Lanquar, Magi El-Richani
  • Production of proteins in glutamine-free cell culture media
    Patent number: 10982003
    Abstract: The present invention relates generally to glutamine-free cell culture media supplemented with asparagine. The invention further concerns the production of recombinant proteins, such as antibodies, in asparagine-supplemented glutamine-free mammalian cell culture.
    Type: Grant
    Filed: May 15, 2019
    Date of Patent: April 20, 2021
    Assignee: Genentech, Inc.
    Inventors: Martin Gawlitzek, Shun Luo, Christina Teresa Bevilacqua
  • Alpha-hemolysin variants and uses thereof
    Patent number: 10968480
    Abstract: Described herein are variants of alpha-hemolysin having at least one mutation, such as a mutation to a positive charge. In certain examples, the mutation is selected from V149K, E287R, H35G, T109K, P151K, K147N, E111N, M113A, or combinations thereof in the mature, wild-type alpha-hemolysin amino acid sequence. The ?-hemolysin variants may also include a substitution at H144A and/or a series of glycine residues spanning residues 127 to 131 of the mature, wild-type alpha hemolysin. Also provided are nanopore assemblies including the alpha-hemolysin variants, the assembly having a decreased time-to-thread. The decreased time-to-thread, for example, increases DNA sequencing efficiency and accuracy.
    Type: Grant
    Filed: May 29, 2019
    Date of Patent: April 6, 2021
    Assignee: Roche Sequencing Solutions, Inc.
    Inventors: Timothy K. Craig, Mark R. Ambroso, Corissa J. Harris, Matthew M. Dipietro, Yaozhong Zou, Marshall W. Porter
  • Method for the fermentative production of molecules of interest by microorganisms comprising genes coding sugar phosphotransferase system (PTS)
    Patent number: 10961499
    Abstract: The present invention relates to a new method for the production of a molecule of interest by conversion of a source of carbon in a fermentative process comprising culturing a microorganism genetically modified for the production of molecule of interest, wherein said microorganism comprises functional genes coding PTS carbohydrate utilization system and wherein the expression of proteins regulated the expression of phosphoenolpyruvate synthase (PPS) is down-regulated. The present invention also relates to the genetically modified microorganism used in the method of the invention.
    Type: Grant
    Filed: July 6, 2017
    Date of Patent: March 30, 2021
    Assignee: METABOLIC EXPLORER
    Inventors: Gwénaëlle Corre, Céline Raynaud
  • Extracellular vesicles with enhanced potency
    Patent number: 10946047
    Abstract: Provided are methods for isolating potent extracellular vesicle or exosome populations from mesenchymal stromal cells, and the use of the isolated extracellular vesicles or exosomes in treating vasculopathy, including pulmonary hypertension, bronchopulmonary dysplasia, and disease and conditions associated with mitochondrial dysfunction.
    Type: Grant
    Filed: May 1, 2018
    Date of Patent: March 16, 2021
    Assignee: United Therapeutics Corporation
    Inventors: Sarah Hogan, Roger Marquez Ilagan, Maria Pia Rodriguez, Carolina Medrano, John Cheadle
  • Animal feed compositions and uses thereof
    Patent number: 10945449
    Abstract: The present invention provides animal feed or animal feed additives comprising one or more polypeptides having lysozyme activity. The invention also provides polypeptides having lysozyme activity, polynucleotides encoding the polypeptides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 4, 2016
    Date of Patent: March 16, 2021
    Assignee: Novozymes A/S
    Inventors: Ye Liu, Kirk Matthew Schnorr, Lars Kiemer, Lars Kobberoee Skov, Dorthe Hoej Sandvang, Marianne Thorup Cohn, Ming Li
  • Compositions and methods for detecting, treating and preventing diseases and disorders
    Patent number: 10941212
    Abstract: The invention described herein relates to the discovery that renalase, and fragments thereof, are useful for the treatment or prevention of cardiac and renal diseases or disorders. Thus, the invention relates to compositions comprising renalase, or fragments thereof, and methods for treating and preventing cardiac and renal disease or disorders.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: March 9, 2021
    Assignee: YALE UNIVERSITY
    Inventor: Gary Desir
  • Lyophilized preparation
    Patent number: 10940185
    Abstract: [Problem] To provide a pharmaceutical composition containing a fusion protein comprising an antibody and a lysosomal enzyme as an active ingredient, which is stable enough to permit its distribution to the market. [Solution] A lyophilized formulation containing; a fusion protein comprising an antibody and a lysosomal enzyme as an active ingredient, and further containing a neutral salt, a disaccharide, a nonionic surfactant, and a buffer. Such a lyophilized formulation includes, for example, as an active ingredient, a fusion protein comprising an anti-transferrin receptor antibody and human iduronate-2-sulfatase, and further containing sodium chloride as the neutral salt, sucrose as the disaccharide, poloxamer as the nonionic surfactant, and phosphate buffer as the buffer.
    Type: Grant
    Filed: December 28, 2017
    Date of Patent: March 9, 2021
    Assignee: JCR Pharmaceuticals Co., Ltd.
    Inventors: Hidehito Yasukawa, Yuka Yamaguchi, Shinji Okabe
  • Strain of bacteria producing DHA and EPA, six gene fragments in the bacterial genome and their applications
    Patent number: 10941185
    Abstract: The present invention discloses a strain of bacteria producing DHA and/or EPA, six gene fragments in the bacterial genome, and uses thereof. The strain is Schizoochytrium limacinum HS01, which has the accession number of CGMCC No. 13746 at China General Microbiological Culture Collection Center. The six gene fragments are composed of gene fragment 1 to gene fragment 6, and the nucleotide sequences are sequentially as shown in SEQ ID NO: 3 to SEQ ID NO: 8 in the Sequence Listing. The experiments prove that fermentation broth containing DHA and EPA can be obtained by fermenting Schizoochytrium limacinum HS01; the recombinant strain is obtained by introducing gene fragment 1 to gene fragment 6 into Schizoochytrium limacinum MYA-1381; the ability the recombinant strain for producing DHA and EPA is greatly improved.
    Type: Grant
    Filed: November 26, 2019
    Date of Patent: March 9, 2021
    Assignee: XIAMEN HUISON BIOTECH CO., LTD.
    Inventors: Liyi Chen, Huichang Zhong, Shuirong Chen
  • Protease inhibitor-containing compositions, compositions comprising same, and methods for producing and using same
    Patent number: 10933022
    Abstract: Provided herein are methods and compositions for oral administration of therapeutic proteins, improved protease inhibitor preparations, methods for producing same, and compositions comprising same.
    Type: Grant
    Filed: May 9, 2019
    Date of Patent: March 2, 2021
    Assignee: Oramed Ltd.
    Inventors: Avraham Hershko, Miriam Kidron
  • Compositions and methods for pesticide degradation
    Patent number: 10913940
    Abstract: Compositions and methods related to anucelate cells (e.g., bacterial minicells) for pesticide degradation applications including related cells, polypeptides, and vectors.
    Type: Grant
    Filed: April 11, 2017
    Date of Patent: February 9, 2021
    Assignee: UNIVERSITY OF VIRGINIA
    Inventors: Payam Pourtaheri, Sepehr Zomorodi, Zachery George Davis, Ameer Hamza Shakeel, Joseph Frank, Shaun Rafie Moshasha, Andrei Khokhlachev, Mark Kester
  • Method for screening agents promoting skin barrier function and method for evaluating skin barrier function taking epidermal serine racemase and/or D-serine level as indicator
    Patent number: 10907208
    Abstract: The purpose of the present invention is to develop a method for screening drugs having the effect of increasing skin barrier function in in vitro studies and to evaluate barrier function in the skin. Candidate drugs can be screened by using the activity and/or expression level of serine racemase as an indicator.
    Type: Grant
    Filed: May 15, 2019
    Date of Patent: February 2, 2021
    Assignee: Shiseido Company, Ltd.
    Inventors: Yosuke Tojo, Masato Iino, Chieko Mizumoto, Yuzo Yoshida, Yuko Matsuura, Hisashi Mori
  • Heparinase-producing strain and heparinase derived therefrom
    Patent number: 10900028
    Abstract: The invention relates to the field of bioengineering. In particular, the invention relates to a heparinase-producing Pseudomonas stutzeri strain and a heparinase derived therefrom. Furthermore, the invention relates to the preparation and use of the heparinase.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: January 26, 2021
    Assignee: Shenzhen Hepalink Pharmaceutical Group Co., Ltd.
    Inventors: Li Li, Jiake Bai, Jinliang Gao, Xiaolai Ma
  • Immuno-based retargeted endopeptidase activity assays
    Patent number: 10900967
    Abstract: The present specification discloses a retargeted endopeptidase pharmaceutical wherein the activity has been determined by the methods disclosed.
    Type: Grant
    Filed: July 8, 2019
    Date of Patent: January 26, 2021
    Assignee: Allergan, Inc.
    Inventors: Joanne Wang, Hong Zhu, Dianne D. Hodges, Ester Fernandez-Salas
  • Recombinant milk proteins
    Patent number: 10894812
    Abstract: Provided herein are compositions and methods for producing recombinant milk proteins, as well as food compositions comprising the same. In aspects, the recombinant milk proteins of the disclosure are recombinant fusion proteins comprising casein and beta-lactoglobulin.
    Type: Grant
    Filed: September 30, 2020
    Date of Patent: January 19, 2021
    Assignee: Alpine Roads, Inc.
    Inventors: Viviane Lanquar, Magi El-Richani
  • Subtilisin variants and uses thereof
    Patent number: 10894954
    Abstract: The present invention relates to subtilisin variants and uses thereof. More specifically, the present invention relates to variant subtilisin Carlsberg sequences and uses thereof.
    Type: Grant
    Filed: September 10, 2019
    Date of Patent: January 19, 2021
    Inventor: Francis Edward Nano