Patents Examined by Karlheinz R. Skowronek
  • Peptides and uses
    Patent number: 9096682
    Abstract: Disclosed herein are zinc finger peptides having at least 4 zinc finger domains, such as 6, 11, 12 or 18 zinc finger domains. The zinc finger peptides may be fused to effector domains for modulating gene expression. Zinc finger peptides of the invention are useful for targeting trinucleotide-repeat sequences in mutant genes and may have applications in gene therapy. The zinc finger peptides may have nucleic acid recognition sequences according to SEQ ID NO: 101. Also disclosed are methods for constructing poly-zinc finger peptides having arrays of at least 8 zinc finger domains, along with zinc finger frameworks that may be useful for selecting zinc finger peptides from libraries.
    Type: Grant
    Filed: October 17, 2011
    Date of Patent: August 4, 2015
    Assignee: FUNDACIO PRIVADA CENTRE DE REGULACIO GENOMICA
    Inventors: Mark Isalan, Mireia Garriga-Canut
  • Opioid prodrugs with heterocyclic linkers
    Patent number: 9095627
    Abstract: The embodiments provide prodrug compounds of Formulae I-XV. The present disclosure also provides compositions, and their methods of use, where the compositions comprise a prodrug compound of Formulae I-XV that provides controlled release of an opioid. Such compositions can optionally provide a trypsin inhibitor that interacts with the enzyme that mediates the controlled release of an opioid from the prodrug so as to attenuate enzymatic cleavage of the prodrug.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: August 4, 2015
    Assignee: Signature Therapeutics, Inc.
    Inventors: Thomas E. Jenkins, Craig O. Husfeld
  • Process for the manufacture of Degarelix and its intermediates
    Patent number: 9090656
    Abstract: The present invention relates to a liquid (or solution)-phase manufacturing process for preparing the decapeptide Degarelix, its protected precursor, and other useful intermediates. The invention further relates to polypeptides useful in the solution-phase manufacturing process and to the purification of Degarelix itself. Degarelix can be obtained by subjecting a Degarelix precursor according to formula (II): (P1)AA1-AA2-AA3-AA4(P4)-AA5-AA6(P6)-AA7-AA8(P8)-AA9-AA10-NH2 (II) or a salt or solvate thereof, to a treatment with a cleaving agent in an organic solvent, wherein P1 is an amino protecting groups; preferably acetyl; P4 is hydrogen or a hydroxy! protecting group, preferably a hydroxyl protecting group; P6 is hydrogen or an amino protecting groups; preferably an amino protecting groups; and P8 is an amino protecting group.
    Type: Grant
    Filed: October 26, 2011
    Date of Patent: July 28, 2015
    Assignee: Ferring B.V.
    Inventors: Jon Holbech Rasmussen, Jens Fomsgaard, Stefan Hansen, Palle Hedengran Rasmussen, Wolfgang Oliver Wachs
  • Injectable solution at pH 7 comprising at least one basal insulin whose PI is between 5.8 and 8.5
    Patent number: 9089476
    Abstract: A composition in the form of an injectable aqueous solution, the pH of which is between 6.0 and 8.0, includes at least a basal insulin, the isoelectric point pI of which is between 5.8 and 8.5; and a dextran substituted by radicals carrying carboxylate charges and hydrophobic radicals. Single-dose formulations at a pH of between 7 and 7.8 includes a basal insulin whose isoelectric point is between 5.8 and 8.5 and a prandial insulin.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: July 28, 2015
    Assignee: ADOCIA
    Inventors: Olivier Soula, Gerard Soula, Jeff Tonnar, Alexandre Geissler
  • Boc and Fmoc solid phase peptide synthesis
    Patent number: 9090654
    Abstract: A solid phase method for synthesizing a peptide containing three or more amino acid residues utilizing both Boc and Fmoc protected amino acids and a chloromethylated polystyrene resin.
    Type: Grant
    Filed: January 4, 2013
    Date of Patent: July 28, 2015
    Assignee: IPSEN MANUFACTURING IRELAND LIMITED
    Inventors: Catherine Fiona Dalton, John Stuart Eynon, Steven Allen Jackson, Gary Alexander Siwruk
  • Formulation for regeneration of bone, cartilage, teeth, and periodontium and treatment of tumors and cysts
    Patent number: 9089580
    Abstract: The present invention provides a composition comprising a corticosteroid and an insulin analog. Further provided is a composition comprising a corticosteroid, insulin lispro and at least one organic acid. The present invention also provides methods of stimulating bone and/or cartilage growth by administering the formulation described herein for the treatment of bone fractures and cartilage damage. Further provided are the methods for the treatment of tumors and cysts of the jaw by administering the formulation described herein.
    Type: Grant
    Filed: February 4, 2015
    Date of Patent: July 28, 2015
    Inventors: Juan Paz Garcia, Brenda Astrid Paz Michel
  • Molecular probe precursor for imaging of pancreatic islet, and use thereof
    Patent number: 9084831
    Abstract: A precursor of a molecular probe for imaging of pancreatic islets is provided.
    Type: Grant
    Filed: March 17, 2011
    Date of Patent: July 21, 2015
    Assignees: Kyoto University, ARKRAY, Inc.
    Inventors: Nobuya Inagaki, Hideo Saji, Kentaro Toyoda, Hiroyuki Kimura, Konomu Hirao, Kenji Nagakawa
  • Stabilized subtilisin composition
    Patent number: 9085750
    Abstract: The conversion of a peptide aldehyde to a hydrosulfite adduct can be used to increase the aqueous solubility in the purification of the peptide aldehydes. Advantageously, this hydrosulfite adduct is itself effective as a subtilisin inhibitor and stabilizer and it can also stabilize a second enzyme if present. The hydrosulfite adduct is effective as a subtilisin inhibitor, and it maintains its inhibitory and stabilizing effect in a liquid detergent during storage. Thus, use of the hydrosulfite adduct can avoid the cost and time of converting it back to the peptide aldehyde and subsequent drying of the peptide aldehyde can be saved, and this can avoid the inconvenience of handling the peptide aldehyde in powder form or as a highly diluted aqueous solution.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: July 21, 2015
    Assignee: Novozymes A/S
    Inventors: Lise Munch Mikkelsen, Francesco Ponzini, Roberto Bisaccia, Renato Canevotti
  • Treatment of biofilms
    Patent number: 9085608
    Abstract: The present invention relates to a peptide or peptidomimetic for use in the treatment of a biofilm-associated infection in a subject, wherein said peptide or peptidomimetic a) carries a net positive charge, —b) is 1 to 6 amino acids in length or is an equivalently sized peptidomimetic; and c) is amphipathic in nature, having one or more lipophilic groups, one of said lipophilic groups comprising at least 7 non-hydrogen atoms.
    Type: Grant
    Filed: October 2, 2009
    Date of Patent: July 21, 2015
    Assignee: LYTIX BIOPHARMA AS
    Inventors: Wenche Stensen, Frederick Alan Leeson, Stig Olov Magnus Engqvist, Trond Flægstad, Øystein Rekdal, John Sigurd Svendsen
  • Preparation of templates for nucleic acid sequencing
    Patent number: 9085802
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: July 21, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, John Milton, Geoffrey Paul Smith, Colin Lloyd Barnes, Isabelle Marie Julia Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu
  • Selective PYY compounds and uses thereof
    Patent number: 9085637
    Abstract: The invention relates to PYY compounds having the amino acid in the position corresponding to position 30 of hPYY(1-36) substituted with tryptophan and derivatives thereof with a modifying group attached to the position corresponding to position 7 of hPYY(1-36). The compounds of the invention are selective Y2 receptor agonists. The invention also relates to pharmaceutical compositions comprising such PYY compounds and pharmaceutically acceptable excipients, as well as the medical use of the PYY compounds.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: July 21, 2015
    Assignee: Novo Nordisk A/S
    Inventors: Soeren Oestergaard, Kilian Waldemar Conde Frieboes, Birgit Wieczorek, Jens Kaalby Thomsen, Birgitte Schjellerup Wulff, Carsten Jessen
  • High throughput screening of ion channels
    Patent number: 9080959
    Abstract: Multi-well plates having contoured well designs allow multi-stage high throughput parallel assaying of ion channels or ion transporters. A well of a multi-well plate has a bottom region that is sized and shaped to simultaneous accommodate a sensing electrode and a pipette for delivering, e.g., test compounds, wash fluid, and optionally ligands. Such multi-well plates may be coupled with an instrument having a pipette head and an electrode plate. Such arrangement facilitates fluidic contact between cells and fluids provided via a pipette. It also facilitates washing of wells with buffers or other wash solutions to allow serial exposure of test cells to various reagents or other stimuli. Generally, the design allows control and test experiments to be performed on the same cell (or cells) in a single well.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: July 14, 2015
    Assignee: Molecula Devices, LLC
    Inventors: Yuri Vladimirovich Osipchuk, James Richard Wasson, Edward D. Verdonk
  • Chemical preparation of polyubiquitin chains
    Patent number: 9079966
    Abstract: The present invention discloses a process for the preparation of a Poly-ubiquitin chain, this process comprising ligating a Ubiquitin thioester Ubm-SR with a Ubiquitin Ubq. Further are disclosed poly-ubiquitin chains prepared according to this process, in particular poly-ubiquitin chains containing at least one unnatural amino acid.
    Type: Grant
    Filed: February 9, 2011
    Date of Patent: July 14, 2015
    Assignee: BEN-GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY
    Inventors: Ashraf Brik, Mahmood Haj-Yahya, Ajish Kumar, Leslie Erlich, Liat Spasser
  • Compositions for labeling nerves and methods of use
    Patent number: 9072773
    Abstract: The present invention provides methods for guiding preservation of neurons or nerves during surgery by administering a fluorescently-labeled peptide that associates with (e.g., specifically binds to) the neurons or nerves. The invention further provides targeting molecules of fluorescently-labeled peptides or aptamers that associate with (e.g., specifically bind to) neurons or nerves and for compositions thereof.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: July 7, 2015
    Assignee: AVELAS BIOSCIENCES, INC.
    Inventors: Jesus Gonzalez, Junjie Liu
  • Propofol formulations with non-reactive container closures
    Patent number: 9072655
    Abstract: A container storing an anesthetic is disclosed. The container is sealed by a closure and stores a liquid anesthetic solution. The anesthetic is from 0.1% to 10% by weight of the liquid anesthetic solution. The container is made of a material that is inert to the anesthetic and the closure is made of siliconized rubber or a metal. A concentration of the anesthetic in a liquid anesthetic solution stored in the container following a predetermined time period is at least 93% of a concentration of the anesthetic in a liquid anesthetic solution before the liquid anesthetic solution is stored in the container.
    Type: Grant
    Filed: April 22, 2014
    Date of Patent: July 7, 2015
    Assignee: FRESENIUS KABI USA, LLC
    Inventors: Neil P. Desai, Andrew Yang, Sherry Xiaopei Ci
  • Reconstituted pulmonary surfactants
    Patent number: 9074018
    Abstract: The present invention is directed to a reconstituted surfactant comprising a phospholipid mixture, and a combination of particular analogues of the native surfactant protein SP-C with analogues of the native surfactant protein SP-B. The invention is also directed to pharmaceutical compositions and kits thereof and to its use for the treatment or prophylaxis of RDS and other respiratory disorders.
    Type: Grant
    Filed: November 20, 2013
    Date of Patent: July 7, 2015
    Assignee: Chiesi Farmaceutici S.p.A.
    Inventors: Jan Johansson, Tore Curstedt
  • Compounds and methods for purifying peptides produced by solid phase peptide synthesis
    Patent number: 9073969
    Abstract: The invention relates to compounds which can be used for purifying peptides produced by solid phase peptide synthesis. In addition, the invention relates to methods for purifying peptides produced by solid phase peptide synthesis using the compounds according to the invention.
    Type: Grant
    Filed: November 16, 2010
    Date of Patent: July 7, 2015
    Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE—CNRS
    Inventors: Vincent Aucagne, Agnes Delmas
  • Protease-activatable pore-forming polypeptides
    Patent number: 9073990
    Abstract: An isolated pore-forming polypeptide is disclosed which comprises a naturally-occurring plugging module and a naturally-occurring pore domain, wherein at least one amino acid of the pore-forming polypeptide is mutated to generate a protease cleavage site, serving to at least partially remove the plugging module from the pore domain. The pore forming polypeptides may be inserted into an encapsulating particle and positioned such that it is capable of forming a pore through the lipid layer of the particle in a presence of the protease.
    Type: Grant
    Filed: April 4, 2011
    Date of Patent: July 7, 2015
    Assignee: Bar-llan University
    Inventors: Yoav Paas, Ilya Pittel, Uri Nir, Barak Ben-David
  • Propofol formulations with non-reactive container closures
    Patent number: 9072656
    Abstract: A container storing an anesthetic is disclosed. The container is sealed by a closure and stores a liquid anesthetic solution. The anesthetic is from 0.1% to 10% by weight of the liquid anesthetic solution. The container is made of a material that is inert to the anesthetic and the closure is made of siliconized rubber or a metal. A concentration of the anesthetic in a liquid anesthetic solution stored in the container following a predetermined time period is at least 93% of a concentration of the anesthetic in a liquid anesthetic solution before the liquid anesthetic solution is stored in the container.
    Type: Grant
    Filed: April 23, 2014
    Date of Patent: July 7, 2015
    Assignee: FRESENIUS KABI USA, LLC
    Inventors: Neil P. Desai, Andrew Yang, Sherry Xiaopei Cl
  • Immobilization of chelating groups for immobilized metal ion chromatography (IMAC)
    Patent number: 9073971
    Abstract: The present invention refers to a method for binding a polycarboxylic acid to a solid phase. Further, the invention refers to a solid phase having a polycarboxylic acid immobilized thereto and methods of using the solid phase, e.g. for purifying His-tagged recombinant polypeptides.
    Type: Grant
    Filed: August 6, 2008
    Date of Patent: July 7, 2015
    Assignee: Max-Planck-Gesellschaft zur Foerderung Der Wissenschaften e.V.
    Inventors: Dirk Goerlich, Steffen Frey