Abstract: Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fungal RS in a transformed host cell.
Type:
Grant
Filed:
June 5, 2001
Date of Patent:
January 27, 2004
Assignee:
E. I. du Pont de Nemours and Company
Inventors:
Paul Veikko Viitanen, Douglas Brian Jordan, Karen Onley Bacot
Abstract: Recombinant host cells that produce rapamycin analogues are constructed by deleting or modifying rapamycin biosynthetic gene cluster genes and are useful in the production of compounds used as antifungals, anticancers, immunosuppressants, and neurotrophins.
Abstract: The present application is directed to a diaminopimelate epimerase from Corynebacterium glutamicum and to polynucleotides encoding this enzyme. The gene has been given the designation “dapF.
Type:
Grant
Filed:
October 27, 1999
Date of Patent:
December 30, 2003
Assignee:
Degussa AG
Inventors:
Bettina Möckel, Walter Pfefferle, Brigitte Bathe, Jorn Kalinowski, Oliver Kirchner, Michael Hartmann, Alfred Pühler
Abstract: Novel polypeptides involved in the biosynthesis of cobalamines and/or cobamides, in particular coenzyme B12, genetic material responsible for expressing these polypeptides, and a method for preparing them, are described. A method for amplifying the production of cobalamines, and particularly coenzyme B12, using recombinant DNA techniques, are also described.
Type:
Grant
Filed:
April 21, 1995
Date of Patent:
December 2, 2003
Assignee:
Rhone-Poulenc Biochimie, et al.
Inventors:
Francis Blanche, Beatrice Cameron, Joel Crouzet, Laurent Debussche, Sophie Levy Schil, Denis Thibaut
Abstract: The present invention related to the novel human kinase polypeptides STLK2, STLK3, STLK4, STLK5, STLK6, STLK7, ZC1, ZC2, ZC3, ZC4, KHS2, SULU1, SULU2, SULU3, GEK2, PAK4, and PAK5, nucleotide sequences encoding the novel kinase polypeptides, as well as various products and methods useful for the diagnosis and treatment of various kinase-related diseases and conditions.
Type:
Grant
Filed:
October 16, 2000
Date of Patent:
December 2, 2003
Assignee:
Sugen, Inc.
Inventors:
Gregory Plowman, Ricardo Martinez, David Whyte
Abstract: There are described methods for making polyhydroxyalkanoate and its copolymers, by culturing a host cell transformed with a vector comprising a polyhydroxyalkanoate biosynthesis-related DNA fragment isolated from Alcaligenes latus. The DNA fragment comprises genes that encode for polyhydroxyalkanoate synthase, &bgr;-ketothiolase, and acetoacetyl-CoA reductase.
Type:
Grant
Filed:
July 11, 2000
Date of Patent:
November 18, 2003
Assignee:
LG Chemical Ltd.
Inventors:
Jong-il Choi, Sang Yup Lee, Kyuboem Han
Abstract: The present invention provides amino acid sequences of human squalene synthase proteins and nucleic acid sequences encoding these squalene synthase proteins. The present invention provides isolated squalene synthase proteins and encoding nucleic acid molecules, vectors and host cells containing these nucleic acid molecules, and processes for producing the squalene synthase proteins.
Type:
Grant
Filed:
March 29, 2001
Date of Patent:
November 18, 2003
Assignee:
Applera Corporation
Inventors:
Ming-Hui Wei, Chunhua Yan, Valentina Di Francesco, Ellen M. Beasley
Abstract: Nucleic acid sequences encoding ∈-cyclase, isopentenyl pyrophosphate isomerase and &bgr;-carotene hydroxylase as well as vectors containing the same and hosts transformed with the vectors. Methods for controlling the ratio of various carotenoids in a host and for the production of novel carotenoid pigments. The present invention also provides a method for screening for eukaryotic genes encoding carotenoid biosynthesis, and for modifying the disclosed enzymes.
Abstract: The present invention relates to polynucleotide molecules comprising nucleotide sequences encoding an aveC gene product, which polynucleotide molecules can be used to alter the ratio or amount of class 2:1 avermectins produced in fermentation cultures of S. avermitilis. The present invention further relates to vectors, host cells, and mutant strains of S. avermitilis in which the aveC gene has been inactivated, or mutated so as to change the ratio or amount of class 2:1 avermectins produced.
Type:
Grant
Filed:
August 10, 2000
Date of Patent:
October 14, 2003
Assignee:
Pfizer, Inc.
Inventors:
Kim J. Stutzman-Engwall, Yan Chen, Claes Gustafsson, Anke Krebber, Jeremy Minshull, Sun Ai Raillard
Abstract: Recombinant host cells that comprise recombinant DNA expression vectors that drive expression of a product and a precursor for biosynthesis of that product can be used to produce useful products such as polyketides in host cells that do not naturally produce the product or produce the product at low levels due to the absence of the precursor or the presence of the precursor in rate limiting amounts.
Abstract: A process for preparing alanine by culturing a genetically modified microorganism that produces alanine from glucose. The microorganism is one that has been modified to result in an overexpression of an endogenous alanine dehydrogenase gene, expression of a heterologous alanine dehydrogenase gene, or a combination of both. The source of alanine dehydrogenase may be Bacillus sphaericus. The modified microorganisms may be a homofermentative microorganism, such as a strain of L. lactis. The process can be used for in situ and stereoscopic preparation of alanine. The invention also relates to the microorganisms and the recombinant DNA molecules that can be used in the invention, as well as to alanine and alanine-containing products obtained according to the process of the invention.
Type:
Grant
Filed:
November 30, 2000
Date of Patent:
September 30, 2003
Assignee:
Nederlands Instituut voor Zuivelonderzoek
Inventors:
Pascal Hols, Michiel Kleerebezem, Oscar Paul Kuipers, Thierry Ferain, Jean Marcelin Alain Marie Ghislain Delcour, Willem Meindert De Vos
Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having alpha-amylase activity [E.C. 3.2.1.1], which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
Abstract: The present invention is directed to nucleotide sequences coding for the superoxide dismutase (sod) gene from Corynebacterium melassecola. It includes processes for the fermentative preparation of nucleotides, vitamins and L-amino acids using coryneform bacteria in which the sod gene is amplified.
Abstract: The present invention pertains, in general, to the identification, isolation and use of Telomerase Reverse Transcriptase (TERT) genes and the proteins encoded by such genes. In particular, the present invention pertains to the identification, isolation and use of TERT genes and TERT proteins from several genetically diverse and economically important organisms, including two human pathogens, Candida albicans and Plasmodium falciparum and an agronomic crop species, Oryza sativa.
Type:
Grant
Filed:
October 13, 1999
Date of Patent:
April 1, 2003
Assignee:
Research & Development Institute, Inc.
Inventors:
David M. Long, Anneke M. Metz, Ruschelle A. Love
Abstract: The invention provides methods and compositions relating to human polypeptide activators of caspases such as polypeptide and polynucleotide sequences diagnostic of caspase activators. These sequences and polypeptides and polynucleotides embodying these sequences find a wide vanety of diagnostic and therapeutic applications involving detecting and/or modulating expression and/or function of activators or caspases or genes or transcripts encoding such activators and generating genetic and immuno probes specific to activators of caspases.
Abstract: Cell-free systems which effect the production of polyketides employing modular polyketide synthases are described. Libraries of new and/or known polyketides may also be produced in cell-free systems employing aromatic PKS, modular PKS or both.
Type:
Grant
Filed:
June 26, 2000
Date of Patent:
March 11, 2003
Assignees:
Stanford University, Brown University Research Foundation
Inventors:
Chaitan Khosla, Rembert Pieper, Guanglin Luo, David E. Cane
Abstract: The present invention describes the DNA sequence for eukaryotic genes encoding &egr; cyclase, isopentenyl pyrophosphate (IPP) isomerase and &bgr;-carotene hydroxylase as well as vectors containing the same and host cells transformed with said vectors. The &egr; cyclase and &bgr;-carotene hydroxylase genes disclosed include those from A. thaliana; the IPP isomerase genes disclosed include those from A. thaliana, H. pluvialis, and marigold. The present invention also provides methods for controlling the ratio of various carotenoids in a host cell and for the production of novel carotenoid pigments. The present invention also provides a method for screening for eukaryotic genes encoding carotenoid biosynthesis enzymes.
Abstract: Hydantoinase enzymes which are mutants of a previously isolated hydantoinase having the amino acid SEQ. ID. NO. 2. The mutants include amino acid substitutions at positions 95, 154, 180, 251 and/or 255 of the wild type hydantoinase (SEQ. ID. NO. 2). The mutant hydantoinases, like the parent hydantoinase, are used in the production of optically pure amino acids.
Type:
Grant
Filed:
February 3, 2000
Date of Patent:
February 25, 2003
Assignee:
California Institute of Technology
Inventors:
Frances H. Arnold, Oliver May, Karlheinz Drauz, Andreas Bommarius
Abstract: Spinosyn biosynthetic genes from Saccharopolyspora spinosa, spinosyn producing microorganisms transformed with the biosynthetic genes, methods using the biosynthetic genes to increase production of spinosyn insecticidal macrolides, and methods using the genes or fragments thereof to change the products produced by spinosyn-producing microorganisms are provided.
Type:
Grant
Filed:
June 23, 2000
Date of Patent:
February 18, 2003
Assignee:
Dow AgroSciences LLC
Inventors:
Richard H. Baltz, M. Christine Broughton, Kathryn P. Crawford, Krishnamurthy Madduri, Donald J. Merlo, Patti J. Treadway, Jan R. Turner, Clive Waldron