Abstract: The invention provides phenylalanyl tRNA synthetase (pheS) pheS polypeptides and DNA (RNA) encoding phenylalanyl tRNA synthetase (pheS) pheS polypeptides and methods for producing such polypeptides from Chlamydia trachomatis by recombinant techniques. Also provided are methods for utilizing pheS polypeptides to screen for antibacterial compounds.

Abstract: The invention provides aspS polypeptides and DNA (RNA) encoding aspS polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing aspS polypeptides to screen for antibacterial compounds.

Abstract: The invention provides phosphoribosyl transferase polypeptides also called adenine phosphoribosyl transferases, from Staphylococcus aureus and DNA (RNA) encoding such phosphoribosyl transferase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing phosphoribosyl transferase polypeptides to screen for antibacterial compounds.

Abstract: Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.

Abstract: The invention provides phenylalanyl tRNA synthetase (pheS) (beta) and pheS (alpha) polypeptides and DNA (RNA) encoding pheS (beta) and pheS (alpha) polypetides from Streptoccus pneumoniae and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pheS (beta) and pheS (alpha) polypeptides to screen for antibacterial compounds.

Abstract: The present invention is directed to compositions and methods for producing avermectins, and is primarily in the field of animal health. The present invention relates to the identification and characterization of two novel genes, herein referred to as the aveR1 and aveR2 genes, that are involved in regulating avermectin polyketide synthase (PKS) expression and avermectin biosynthesis in Streptomyces avermitilis. The present invention is based on the discovery that inactivation of these genes results in an increase in the amount of avermectin produced by S. avermitilis.

Abstract: A method for the production of D-pantothenic acid by the fermentation of microorganisms of the family Enterobacteriacae producing D-pantothenic acid which is characterized in that strains are used which a) Contain the plasmid pFV31 and/or pFV202 and that b) A panD gene and optionally other nucleotide sequences coding for aspartate-1-decarboxylase are enhanced, especially overexpressed in these microorganisms, c) The pantothenic acid is enriched in the medium or in the cells of the microorganisms and d) The pantothenic acid formed is isolated.

Abstract: The present invention relates to isolated polypeptides having pectin acetylesterase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Abstract: The present invention relates to a method of designing laccase mutants with increased oxidation potential and/or changed pH optimum and/or altered mediator pathway and/or altered O2/OH−-pathway, which method is based on the hitherto unknown three-dimensional structure of laccases.

Abstract: Yields of polyketides produced in host cells such as Streptomyces can be increased by coexpression of the ptpA gene. The introduction of recombinant vectors encoding ptpA and polyketide synthase genes is more efficient and does not require methyl-free DNA if the host cell is a restriction/methylation deficient strain, such as Streptomyces lividans K4-114, K4-155, or K27-39.

Abstract: The invention discloses three polynucleotide sequences for the fermentative production of D-pantothenic acid. These polynucleotide sequences are genes named panB, encoding a ketopantoate hydroxymethyltransferase, panC, encoding pantothenate synthase, and ilvD, encoding dihydroxy-acid dehydratase. The genes panB and panC are found on the same operon, panBC, while the gene ilvD is found in a separate operon. These genes can be used separately or together to enhance the production of D-pantothenic acid in microorganisms, especially in Corynebacterium.

Abstract: The invention provides lysS polypeptides and DNA (RNA) encoding lysS polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing lysS polypeptides to screen for antibacterial compounds.

Abstract: This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of soybean glutathione-S-transferase (GST) enzymes involved in the detoxification of xenobiotic compounds in plants and seeds. The invention also relates to the construction of chimeric genes encoding all or a substantial portion of soybean GST enzymes, host cells transformed with those genes and methods for the recombinant production of soybean GST enzymes. Methods of constructing transgenic plants having altered levels of GST enzymes and screens for identifying soybean GST enzyme substrates and soybean GST enzyme inhibitors are also provided.

Abstract: Compositions and methods are provided for the enhanced in vitro synthesis of biological molecules where ATP is required for synthesis. Of particular interest is the synthesis of polymers, e.g. nucleic acids, polypeptides, and complex carbohydrates. A homeostatic system is used for production of ATP, where the required high energy phosphate bonds are generated in situ, e.g. through coupling with an oxidation reaction. The homeostatic energy source will typically lack high energy phosphate bonds itself, and will therefore utilize free phosphate in the reaction mix during generation of ATP. Since inorganic phosphate can be an inhibitory by-product of synthesis, the period of time when synthesis is maintained in vitro can be extended. The homeostatic energy source is provided in combination with an enzyme that catalyzes the creation of high energy phosphate bonds and with an enzyme that can use that high energy phosphate bond to regenerate ATP.

Abstract: The invention provides Tryptophanyl tRNA Synthetase polypeptides and DNA (RNA) encoding Tryptophanyl tRNA Synthetase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Tryptophanyl tRNA Synthetase polypeptides to screen for antibacterial compounds.

Abstract: An antigenically active hybrid glutamic acid decarboxylase (GAD) comprising an amino-terminal moiety derived from the GAD67 isoform linked directly or indirectly with a middle and carboxy-terminal moiety derived from the GAD65 isoform, and production thereof as a recombinant protein by expression in eukaryotic host cells, particularly yeasts.

Abstract: The invention provides tRNA methyl transferase (trmD) polypeptides and polynucleotides encoding trmD polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing trmD polypeptides to screen for antibacterial compounds.

Abstract: The invention provides phenylalanyl tRNA synthelase, pheS, (alpha) polypeptides and DNA (RNA) encoding pheS (alpha) polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing pheS (alpha) polypeptides to screen for antibacterial compounds.

Abstract: The present invention relates to a biosynthetic process for preparing cobalamines. More precisely, it relates to a process for amplifying the production of cobalamines and, more specifically, of coenzyme B.sub.12 by means of recombinant DNA techniques and/or by means of adding a novel cobalamine precursor.

Abstract: The invention provides a human cytochrome P450 (HUCYP) and polynucleotides which identify and encode HUCYP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with expression of HUCYP.