Abstract: A carbon dioxide-neutral biodigester plant includes a biodigester for carbon dioxide-neutral, single-stage or multi-stage production of biogas by fermentation of biomass in a fermentation liquid agitated in presence of elemental hydrogen, hydrogenotrophic and methanogenic archaea, and activated carbon masses, a source of ammonia connected to a first ammonia line, an ammonia cracker to produce hydrogen or a hydrogen-nitrogen mixture by catalytic cracking of ammonia, a first hydrogen line for feeding the produced hydrogen or the hydrogen-nitrogen mixture directly into the biodigester, and a treating device for treating activated coal masses with hydrogen at higher pressure. The treating device includes a pressure line and a treating spray lance. Hydrogen-containing activated coal masses configured as spray cones are injected into the fermentation liquid of the biodigester.

Abstract: A microfluidic cell culture system is provided. The system includes a dual circulation arrangement for providing the cell culture with culture medium (and, optionally, selected compounds for study). The dual circulation arrangement permits culture conditions to be readily modified for different phases of cell culture. In particular, a first circulation route can be used to circulate a relatively high volume of medium, thereby allowing a low cell number to medium volume ratio, and a second circulation route can be used to circulate a relatively low volume of medium, thereby allowing a high cell number to medium volume ratio. The first circulation is optimised for a pre-culture period, before test compounds are added, and the second circulation is optimised for the test phase, providing a high cell number to medium ratio while preserving function during the test period.

Abstract: The present invention relates to a reactor comprising a vessel (1) for containing: ?a mass to be treated, and ?at least one lighting device (2a, 2b) intended to promote the treatment of said mass, characterized in that each lighting device (2a, 2b) comprises a light diffuser including at least one micro-etched plate (211) which is transparent to light radiation.

Abstract: An isolation chip assembly includes an isolation chip (10), first oscillators (20), and second oscillators (30). The isolation chip (10) includes a sample reservoir (13), a first filtration membrane (14) and a second filtration membrane (16) at opposite sides of the sample reservoir (13). The first oscillators (20) are mounted on the first filtration membrane (14) and the second filtration membrane (16), and can generate a first oscillation wave when operating. The second oscillators (30) are mounted on outer surfaces of the first chamber (15) and the second chamber (17), and can generate a second oscillation wave when operating. A frequency of the first oscillation wave is greater than a frequency of the second oscillation wave, and an amplitude of the first oscillation wave is less than an amplitude of the second oscillation wave.

Abstract: This invention is directed to a method for three-dimensional cultivation of proliferating plant cells in a packed bed bioreactor, the bioreactor comprising at least one packed bed chamber configured to confine said proliferating plant cells within it such that the proliferating plant cells become a three-dimensional stationary biomass phase; and At least one container comprising a fluid media, the fluid media is configured to flow through said proliferating plant cells stationary biomass phase, wherein, the flow of the fluid media through the proliferating plant cells stationary biomass phase allows transfer of compounds from the fluid media into the cells and vice versa in low shear forces within the at least one packed bed chamber thereby imitating natural growth environment of the proliferating plant cells within the 3D bioreactor.

Abstract: A kit of parts for sealing openings between an enclosure extending through a wall separating a cleanroom and a space outside the cleanroom. Multiple gaskets, frame members, and a lower skirt are positioned to seal any openings between the side, top and bottom surfaces of the enclosure and proximate wall surfaces. The sealing components can be easily installed without the use of tools.

Abstract: A cell cultivation apparatus for cultivating microorganisms and growing cells at high density is provided. The apparatus includes a membrane comprising multiple surface features on a first side of the membrane for cell placement. The surface features comprising one or more compartments within which a cell can be located. The membrane includes a material that is at least partially permeable to gas. A second side of the membrane defines a gas region. The second side of the membrane is separated from the first side of the membrane by the membrane. The apparatus further includes a media region for receiving media. The compartments are configured to at least partially reduce media flow shear forces on one or more cells in the compartments. The surface features may be ridges, protrusions, fins, wells, and/or posts.

Abstract: A system and method for growing microalgae capable of mixotrophic metabolism, preferably Chlorella sp. Microalgae grown in the system using the method are able to survive and grow in dark refrigeration, which allows the algae to be stored and transported for application as a live culture. In addition, the microalgae can be grown in sufficient quantities to be sold commercially for application to crops as a biostimulant.

Abstract: A cover for a slurry container is provided. The cover includes a plurality of attachable cover units including a first attachable cover unit and a second attachable cover unit. Each of the attachable cover units has at least one edge with an attachable portion. The first attachable cover unit is attached to the second attachable cover unit at the edge of the first and second attachable cover units having the attachable portion.

Abstract: A screen changing device is proposed. The screen changing device includes a first housing and a second housing configured to be rotated with respect to the first housing. The screen changing device can transit from a first configuration in which a first screen is aligned with a first connector and a second connector to a second configuration in which the first screen is not aligned with the first connector and the second connector and a second screen is aligned with the first connector and the second connector.

Abstract: The current invention relates to a bioreactor cabinet, configured to be incorporated in a biomolecule production system, wherein said bioreactor cabinet is preferably a mobile bioreactor cabinet suited to receive a bioreactor, said bioreactor cabinet is provided with a bioreactor docking station, wherein said bioreactor cabinet, more preferably a side wall of said bioreactor cabinet, is provided with a connector allowing the transmission of power, signals and/or data when paired with a biomolecule production system, such as a bioreactor chamber of said system. The current invention equally relates to a biomolecule production system and methods for producing biomolecules.

Abstract: A cell culture device is provided. A cell culture device according to an exemplary embodiment of the present invention includes: a main body comprising a culture space having one surface that is open; and a surface-modified sheet-type culture plate which is fixed to the main body to cover the opened one surface of the culture space, and thus forms a culture surface on which cells are cultured, and which forms the culture surface so that the cells can be attached to the culture surface.

Abstract: A cartridge for facilitating automation of a vessel includes a cartridge body, an adapter for removably connecting the vessel to the cartridge body, and at least one connection unit for fluidic communication with the vessel. A connection unit includes a retainer, a port body, a connector, and a tube. The retainer is fixed on or integrally formed with a wall of the cartridge body. The port body is coupled to the retainer and has a distal end portion positioned exterior to the cartridge body. The connector is housed by the port body and has a first end and a second end, where the first end is positioned outside of the cartridge body. The tube selectively connects the second end of the connector to a port of the vessel.

Abstract: A drug screening device is provided. A method of determining optimal drug concentrations and efficacy in a patient using the device are provided. A method of determining effective chemotherapeutic drugs and effective concentrations thereof using the device is provided. Also, a method of determining safety and efficacy of drugs using the device is provided.

Abstract: Disclosed is a biological sample storage and retrieval apparatus, comprising a storage device, with the interior thereof being a cryogenic environment for storing a biological sample, and the biological sample comprising cryogenic vials and a cryogenic shelf for holding the cryogenic vials; an operation device detachably connected to the storage device for receiving a transfer tank for holding the biological sample and performing storage and retrieval of the biological sample in the storage device by means of the transfer tank; and a sealing device, the sealing device being used for sealing the storage device when the storage device is detached from the operation device. A cryogenic storage and retrieval method is also provided.

Abstract: Electroporation is a technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane. This allows for chemicals, drugs, and/or macromolecules such as proteins and nucleic acids to be introduced into the cells. Embodiments relate to an electroporation apparatus. The electroporation apparatus comprises: a plurality of chambers configured to store a plurality of cells during an electroporation process; a plurality of electrodes configured to generate a plurality of electric fields within the plurality of chambers during the electroporation process, each electric field of the plurality of electric fields corresponding to one chamber of the plurality of chambers; a flow channel configured to transport the plurality cells during a cell collection process after the electroporation process; and a plurality of valves connecting the plurality of chambers to the flow channel.

Abstract: A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions.

Abstract: A sensor unit has sensors for measuring the components of a culture medium in a culture vessel, and comprises a plurality of sensors and a linking portion. The plurality of sensors each have a main body and a sensing unit disposed on the lower end side of the main body and immersed in the culture medium to measure the components of the culture medium. The linking portion links the plurality of sensors on the upper end side of the main body.

Abstract: A carbon dioxide capture and sequestration system comprises a growth module, a drying module, a sequestration module and a carbon dioxide recapture apparatus. The carbon dioxide recapture apparatus includes at least one conduit coupled to the growth module, the drying module, and the sequestration module for routing carbon dioxide liberated from at least one of the harvested algal biomass within the drying module and the dried algal biomass withing the sequestration module to the growth module.

Abstract: A radial flow processing device includes a body with an inner chamber, a pair of inner and outer concentric tubes extending into the body, and a processing disk containing a central opening through which the inner tube extends, the disk being connected with the inner tube. The body has a top wall, a bottom wall, and at least one side wall which define the inner chamber. The bottom wall, top wall, or both, contain at least one opening through which at least one tube extends. A diameter of the inner tube is less than a diameter of the outer tube such that there is a space between both tubes, and a diameter of the disk is less than a width of the body.