Abstract: The invention provides a structured cell growth matrix or assembly comprising a one or more spacer layers and one or more cell immobilization layers. The invention further provides a bioreactor comprising said matrix or assembly.

Abstract: Disclosed devices for recovering cryopreserved tissue can comprise a receptacle that receives a sealed, frozen tissue container containing cryopreserved tissue and cryopreservation media, with at least one recovery media chamber and a waste material chamber fluidly coupled to the tissue container receptacle. The recovery device can be inserted into a regulator apparatus that facilitates thawing and warming of the media and tissue, and regulation of the flow of recovery media through the tissue container to flush out the thawed cryopreservation media into the waste chamber. The regulator can identify the tissue based on an ID tag on the tissue container and automatically apply an appropriate algorithm for thawing, culturing, and maintaining the tissue in a viable state.

Abstract: A cell culture device includes a control unit that includes a first gas supply source, a second gas supply source, and a decompression source, and a culture unit that includes a first container, a second container, a first gas channel that connects a third container, the first gas supply source, the second container to a connection point, a second gas channel, a third gas channel, a first liquid channel, a second liquid channel, a gas-liquid channel, a first valve, a second valve, a third valve, a fourth valve, and a fifth valve.

Abstract: Disclosed is a device and a method for storing and transporting at least one human or animal tissue with a view to transplantation or ex vivo experimentation. The device can store such tissue in a specific storage medium until the transplantation or the ex vivo experimentation, and in particular to storing a sample of human or animal cornea, such as a corneal graft. This device for storing at least one human or animal tissue comprises at least one chamber which is suitable for receiving and storing the tissue in a liquid storage medium and which comprises means for continuous renewal of the medium in said at least one chamber. The device additionally comprises means for adjusting at least one physicochemical parameter of said medium, including its pH, by continuous circulation of a buffer gas in said at least one chamber outside of and in contact with said medium.

Abstract: The present invention relates to a culture vessel for three-dimensional cell cultivation and a three-dimensional cell co-cultivation method using the same. The culture vessel comprises a well formed by a column positioned thereon and at least one support protruding from the column within the well. In contrast to conventional techniques, the present invention allows cells to be cultured at a position spaced from a culture vessel, thus enjoying the advantage of smoothly supplying oxygen necessary for three-dimensional cell culture structures.

Abstract: A thin-walled microplate suitable for use in the Polymerase Chain Reaction (PCR) technique comprising a plurality of thin-walled tubes or wells arranged in a fixed array, each well having an upper portion with an open top and a lower, frustoconical portion having a substantially flat bottom.

Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.

Abstract: A water filtering system includes providing light deep into a liquid used in applications such as the cultivation of both attached and suspended photosynthetic organisms. The system is configured to inject a stream of bubbles into the liquid and a light source is configured to shines light through the bubbles. The bubbles allow some light to travel deep into the liquid along the length of the bubble stream and scatter/deflect some light out of the bubble stream and into the surrounding liquid including screens for growing algae.

Abstract: The present invention discloses means, use and non-GM method for modulating proteins in microorganisms. This method comprising steps of providing a system comprising a plasma discharge source, the plasma; the plasma discharge electric field is in the range of about 200 to about 500 v/m; and a plasma modifying mechanism comprising (1) at least one magnetic material, and at least one piezoelectric material, and (2) at least one optical crystal material, the plasma modifying mechanism providing modified plasma output having frequencies in the range of about 3 KHz to about 30 KHz; and discharging the plasma towards the microorganisms in a pulsed profile; thereby modulating proteins from the target microorganisms. The invention also discloses means, use and non-GM method for de novo generating of proteins in microorganisms from within the proteome of the microorganisms.

Abstract: Disclosed herein are devices, methods and compositions for making and using microfluidic devices comprising making a microfluid device comprising one or more channels; and coating at least a portion of an interior surface of at least one channel, wherein one or more gradients are formed by simultaneously introducing into the device a first cellular culture media composition having a certain concentration of a gas, an active agent or both and a second composition having a concentration of a gas, an active agent or both that is/are different from that of the first composition. Such devices are used to investigate cellular responses to physiological states and active agents.

Abstract: A living body specimen transport device for receiving multiple living body specimens has a frame, a rotating bracket, and a storage assembly. The rotating bracket can be rotated with respect to the frame. The storage assembly can receive a container with a living body specimen and be rotated with respect to the rotating bracket. A center of gravity of the storage assembly is lower than a pivoting point where the rotating bracket is mounted on the frame and a pivoting point where the storage assembly is mounted on the rotating bracket. With such structure, even when the living body specimen transport device is vibrated and shaken during transporting and then the frame of the living body specimen transport device is tilted or turned over, the rotating bracket and the storage assembly can rotate to be vertical by themselves, which keeps the living body specimen being soaked in the preservation solution.

Abstract: The present disclosure relates to a biocompatible, in vitro probe system. The probe system may have a substrate and a culture well supported on the substrate. The culture well defines a three-dimensional volume for containing in vitro cultures of electroactive cells. The probe system has at least one probe subsystem supported on the substrate. The probe subsystem has at least one probe having an array of electrodes, with the probe being disposed within the culture well for in vitro electrically communicating with the electroactive cells. The probe subsystem is adapted to be interfaced to an external instrumentation/recording device.

Abstract: A cell bag rotator is constructed to slowly agitate a plurality of cell bags by rotation along a rotation axis that is at least partially horizontal. The cell bag rotator comprises a plurality of plates to which a respective cell bag is attached. The cell bag rotator is suitable for mixing cells with particles for performing various types of subsequent cell therapy processes on the cells.

Abstract: A food recycler can include a housing defining an interior and an access opening to the interior. A mixing bin can be located within the interior and can have an inlet and an outlet. The food recycler can further include an output bin, the output bin having an inlet. The outlet of the mixing bin can couple or connect to the inlet of the output bin. The food recycler further has a transfer assembly for transferring food waste from the mixing bin to the output bin. The transfer assembly includes a chute mounted to the housing and operably coupling the first outlet of the mixing bin to the second inlet of the output bin, and at least one vibration damper positioned between the housing and the chute configured to allow flexibility of the chute to vibrate relative to the housing.

Abstract: An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

Abstract: A device for freezing adipose tissue comprises a first member and a second member which are mutually coupleable so as to obtain at least one closed configuration; the first member comprising at least one manual grip portion and at least one support portion to which the adipose tissue adheres; the second member comprising at least one housing wherein at least the support portion of the first member is insertable. The second member comprises at least one safety valve that comprises at least one separable portion adapted to detach, partially or completely, from the body of said second member in the case the pressure difference between the inside and the outside exceeds a predetermined value. The second member comprises retention means of the separable portion once it has detached. The detachment of the separable portion creates an opening in the second member, and a gap is defined for the discharge of the gases contained within the housing.

Abstract: The present invention provides a cell culture base that avoids contact between a photoresponsive layer and cells. The cell culture base of the present invention includes: a support layer; a photoresponsive layer; and a cell culture layer, wherein the photoresponsive layer is laminated on the support layer, the cell culture layer is laminated on the photoresponsive layer, the photoresponsive layer contains a photoresponsive substance that causes at least one of heat and change in magnetism by light irradiation, and the cell culture layer is capable of culturing cells.

Abstract: A system for cryocooling biological samples, including a first chamber configured to hold a first amount a cryogenic liquid; a container holder positioned in thermal contact with the first chamber and configured to hold at least one removable container positioned therein, wherein the container is configured to hold a second amount of the cryogenic liquid and forms a second chamber; an elongated tube holder configured to hold at least one hollow elongated tube into the container; and a sample wand configured to hold and transfer at least one sample holder with a biological sample into the elongated tube while the elongated tube is in the container with the second amount of the cryogenic liquid therein.

Abstract: An apparatus comprising a culture dish and a removable lid, wherein the culture dish comprises a main body having a side wall defining a reservoir region for receiving a quantity of liquid media, and the removable lid is arranged to cover the reservoir region during normal use, wherein the lid comprises a gas permeable material and includes an engagement portion formed of a resilient material adapted to cooperatively engage with the side wall of the main body of the culture dish so as to compress a part of the engagement portion of the removable lid against the side wall to form a vapour-tight seal for the reservoir region when the removable lid is coupled to the culture dish. The lid fitted to the culture dish enables a substantial portion of the culturing media to remain in the environment enclosed between the reservoir and the lid without use of a cover media to limit evaporation while allowing gaseous exchange therethrough.

Abstract: Disclosed is an in vitro exposure system that may radiate a uniform field having a constant wavefront to an experimental cell container and expose each cell container to a same electromagnetic field.