Patents Examined by Lisa J. Hobbs
  • Patent number: 7517667
    Abstract: In vitro delivery of the diphtheria toxin (DT) catalytic (C) domain from the lumen of purified early endosomes to the external milieu requires the addition of both ATP and a cytosolic translocation factor (CTF) complex. The results presented here demonstrate that ?-COP plays an essential role in the cytosolic release of the C-domain and is mediated by a consensus peptide sequence found on several bacterial toxins and in HIV-1 reverse transcriptase. The invention features methods for inhibiting cell death that include the administration of compounds based on this consensus sequence that inhibit the translocation of the catalytic domain of toxins or transcription factors. Also featured are methods for identifying compounds that inhibit cell death, and methods for identifying compounds that promote cell death by blocking or accelerating, respectively, the rate of toxin/factor endosomal translocation.
    Type: Grant
    Filed: August 30, 2005
    Date of Patent: April 14, 2009
    Assignee: Boston Medical Center Corporation
    Inventors: John R. Murphy, Ryan Ratts
  • Patent number: 7514235
    Abstract: Disclosed is a medium for the detection and/or identification of a Candida yeast, the medium comprising: a chromogen; carbohydrate in the range 1-5 gms/liter; and an alcohol; the medium being such that growth of the Candida yeast under appropriate conditions results in hydrolysis of the chromogen to generate a chromophore of a derived color which is a different color from that generated by hydrolysis of the chromogen in a standard medium.
    Type: Grant
    Filed: December 10, 2004
    Date of Patent: April 7, 2009
    Assignee: Oxoid Limited
    Inventor: Bryan Stevon De Caux
  • Patent number: 7445777
    Abstract: A pharmaceutical kit for treatment of retinitis pigmentosis and a method of producing the same, the kit comprising the enzymes glutathione peroxidase, prolidase, glucose-6-phosephate dehydrogenase and, optionally, aldose reductase in aliquot parts and interactive quantities appropriate for administering the enzymes in accordance with a predetermined time sequence.
    Type: Grant
    Filed: October 1, 2002
    Date of Patent: November 4, 2008
    Inventors: Paola Ammannati, Roberto Giordani
  • Patent number: 7429464
    Abstract: The present invention provides for methods for detecting bacterial pathogens in a sample. A preferred method includes the steps of: suspending a sample comprising a medium and microorganisms, the microorganisms suspected of comprising bacterial pathogens; mixing the sample, an adsorbent and a first solution in a vessel; separating the adsorbent and the microorganisms from the medium and the bulk of the first solution and removing the medium and the bulk of the first solution from the vessel; adding a second solution to the vessel to resuspend the adsorbent and the microorganisms; and detecting agglutination of the adsorbent wherein the agglutination of the adsorbent signifies bacterial pathogens are present in the sample.
    Type: Grant
    Filed: December 7, 2004
    Date of Patent: September 30, 2008
    Assignee: bioMerieux, Inc.
    Inventors: John D. Walsh, Jones M. Hyman
  • Patent number: 7425411
    Abstract: The present invention provides for a method for recovering microorganisms from a sample that is rapid and provides microorganisms substantially free of adsorbents and other particles. The method includes mixing a sample comprising a medium, an adsorbent and microorganisms with a first solution in a first vessel; separating the adsorbent from the medium and the microorganisms and removing the microorganisms and a portion of the medium from the first vessel; mixing the microorganisms and the portion of the medium with a second solution in a second vessel to resuspend the microorganisms; and separating the microorganisms from the resulting mixture.
    Type: Grant
    Filed: December 9, 2004
    Date of Patent: September 16, 2008
    Assignee: bioMerieux, Inc.
    Inventor: John D. Walsh
  • Patent number: 7413886
    Abstract: It is intended to provide a process for conveniently producing phosphorylase at a high purity using a phosphorylase-producing microorganism. Namely, a process for producing phosphorylase characterized by culturing a phosphorylase-producing microorganism in a medium containing phosphoric acid or its salt at a concentration of 50 mM or above and collecting the phosphorylase thus produced in the medium.
    Type: Grant
    Filed: June 4, 2004
    Date of Patent: August 19, 2008
    Assignee: Kao Corporation
    Inventors: Kazuaki Igarashi, Shuuichi Takizawa, Norihiko Higaki, Jun Hitomi
  • Patent number: 7399609
    Abstract: The invention relates to methods of detecting staphylococcus.
    Type: Grant
    Filed: October 7, 2004
    Date of Patent: July 15, 2008
    Assignee: 3M Innovative Properties Company
    Inventors: Brinda B. Lakshmi, Angela K. Dillow, M. Benton Free, John S. Huizinga, Patrick A. Mach, Dasaratha V. Sridhar
  • Patent number: 7390641
    Abstract: A process for the production of peroxidase which comprises of establishing a plant cell culture producing cells from neem (Azardiracta indica) and nirgundi (Vitex negundo) wherein the peroxidase has higher enzymatic activity not reported earlier.
    Type: Grant
    Filed: May 24, 2005
    Date of Patent: June 24, 2008
    Assignee: Council of Scientific and Industrial Research
    Inventors: Tholath Emilia Abraham, Nisha Rani Devaki, Thomson Kuruvilla, Jegan Roy Joseph
  • Patent number: 7374901
    Abstract: The present invention relates to an analysis method and analysis system that can accurately analyze the contents of coenzyme Q-10 and a 2-electron reduced form thereof in a specimen, in which human blood plasma as the specimen is mixed with isopropyl alcohol as a pretreatment and the coenzyme Q-10 and the 2-electron reduced form thereof are extracted to the isopropyl alcohol. Extracted liquid is stored at a temperature of 4° C. until the analysis is performed. The extracted liquid as an analytical sample is analyzed by an analysis system provided with a liquid-sending mechanism, a switching mechanism, a concentration column, a separation column, a reduction column, an ultraviolet absorption detector, and an electrochemical detector.
    Type: Grant
    Filed: December 3, 2003
    Date of Patent: May 20, 2008
    Assignee: Shiseido Company Ltd.
    Inventors: Yorihiro Yamamoto, Masashi Mita
  • Patent number: 7341857
    Abstract: The present invention provides a novel esterase derived from Ideonella sp. 0-0013 strain (FERM BP-08660) having the following properties: (1) function, substrate specificity: hydrolyzes methyl 3-hydroxypalmitate to generate 3-hydroxypalmitic acid and methanol; (2) optimal temperature for functioning: 37° C.; (3) optimal pH and stable pH range: pH 7 or more to pH 10 or less; (4) temperature stability: 97% of the enzyme is stable at 43° C.; (5) inhibition, activation, and stabilization: activated by sodium ion and potassium ion, and inhibited by strontium ion, iron ion (divalent), and methyl palmitate; (6) molecular weight: about 46,500 Da (by SDS-PAGE), about 41,000 Da (by a gel filtration method); and (7) isoelectric point: pI 4 (by polyacrylamide gel isoelectric focusing method); a microorganism producing the enzyme; and a method of producing the enzyme.
    Type: Grant
    Filed: March 16, 2004
    Date of Patent: March 11, 2008
    Assignee: Incorporated Administrative Agency National Agriculture and Bio-Oriented Research Organization
    Inventors: Makoto Shinohara, Yoichi Uehara, Akimasa Nakano
  • Patent number: 7329520
    Abstract: The present invention is directed to a defructosylation enzyme originating from a plant, a method of defructosylating a fructosylated peptide or protein through use of the enzyme, and a method of measuring a fructosylated peptide or protein.
    Type: Grant
    Filed: October 23, 2003
    Date of Patent: February 12, 2008
    Assignee: Daiichi Pure Chemicals Co., Ltd.
    Inventor: Hiroyuki Ebinuma
  • Patent number: 6537803
    Abstract: The present invention relates to novel mammalian amino acid transporter proteins and the genes that encode such proteins. The invention is directed toward the isolation, characterization and pharmacological use of the human amino acid transporter proteins EAAT1, EAAT2, EAAT3 and ASCT1. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to each of these transporter genes. Also provided are recombinant expression constructs capable of expressing each of the amino acid transporter genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the human amino acid transporter proteins encoded therein. The invention also provides methods for screening in vitro compounds having transport-modulating properties using preparations of transporter proteins from such cultures of cells transformed with recombinant expression constructs.
    Type: Grant
    Filed: January 8, 1999
    Date of Patent: March 25, 2003
    Assignee: Oregon Health Sciences University
    Inventors: Susan G. Amara, Jeffrey L. Arriza
  • Patent number: 6423684
    Abstract: The present invention relates to a polypeptide composition effective to alter the activity of a first protein that interacts with a second protein, where the second protein contains at least one WD-40 region. The polypeptides of the present invention typically have between 4 and 50 amino acids whose sequence is the same as a sequence of the same length in the WD-40 region of the second protein. The invention further includes a method of altering the activity of the above described first protein. In one embodiment of the invention the polypeptide composition is effective to alter the activity of a protein kinase C, where the protein kinase C interacts with a second protein, and the second protein contains at least one WD-40 region (e.g., RACK1).
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: July 23, 2002
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Daria Mochly-Rosen, Dorit Ron
  • Patent number: 6423524
    Abstract: The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.
    Type: Grant
    Filed: November 10, 1998
    Date of Patent: July 23, 2002
    Assignee: Novo Nordisk A/S
    Inventors: Fred Hagen, Carsten Mailand Hjort, Sven Hastrup
  • Patent number: 6410285
    Abstract: The invention provides alaS polypeptides and DNA (RNA) encoding alaS polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing alaS polypeptides to screen for antibacterial compounds.
    Type: Grant
    Filed: January 14, 1998
    Date of Patent: June 25, 2002
    Assignees: SmithKline Beecham Corporation, SmithKline Beecham plc
    Inventor: Elizabeth Jane Lawlor
  • Patent number: 6410286
    Abstract: The invention provides tRNA synthetase polypeptides and DNA (RNA) encoding tRNA synthetase polypetides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing tRNA synthetase polypeptide for the protection against infection, particularly bacterial infections.
    Type: Grant
    Filed: February 4, 1998
    Date of Patent: June 25, 2002
    Assignee: SmithKline Beecham p.l.c..
    Inventors: John Edward Hodgson, Elizabeth Jane Lawlor
  • Patent number: 6342368
    Abstract: The present invention relates to a polypeptide composition effective to alter the activity of a first protein that interacts with a second protein, where the second protein contains at least one WD-40 region. The polypeptides of the present invention typically have between 4 and 50 amino acids whose sequence is the same as a sequence of the same length in the WD-40 region of the second protein. The invention further includes a method of altering the activity of the above described first protein. In one embodiment of the invention the polypeptide composition is effective to alter the activity of a protein kinase C, where the protein kinase C interacts with a second protein, and the second protein contains at least one WD-40 region (e.g., RACK1).
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: January 29, 2002
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Daria Mochly-Rosen, Dorit Ron
  • Patent number: 6262023
    Abstract: The present invention relates to a polypeptide composition effective to alter the activity of a first protein that interacts with a second protein, where the second protein contains at least one WD-40 region. The polypeptides of the present invention typically have between 4 and 50 amino acids whose sequence is the same as a sequence of the same length in the WD-40 region of the second protein. The invention further includes a method of altering the activity of the above described first protein. In one embodiment of the invention the polypeptide composition is effective to alter the activity of a protein kinase C, where the protein kinase C interacts with a second protein, and the second protein contains at least one WD-40 region (e.g., RACK1).
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: July 17, 2001
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Daria Mochly-Rosen, Dorit Ron
  • Patent number: 6245546
    Abstract: The present invention relates to animal feed additives, which additives comprise a monocomponent xylanase derived from a strain of Byssochlamus, Chaetomium, Humicola, Malbranchea, Mucor, Myceliophthora, Paecilomyces, Talaromyces, Thermoascus, or Thielavia. In other aspects, the invention relates to monocomponent xylanase preparations, DNA constructs, recombinant expression vectors, host cells, and methods of producing monocomponent xylanase preparations.
    Type: Grant
    Filed: July 15, 1998
    Date of Patent: June 12, 2001
    Assignee: Novo Nordisk A/S
    Inventors: Peter Kamp Hansen, Peter Wagner, Anette Mullertz, Inge Helmer Knap
  • Patent number: 6221640
    Abstract: Recombinant nucleic acids which encode aminoacyl-tRNA sythetases of enterococcal origin or portions of such enzymes, have been isolated. These nucleic acids can be used to make expression constructs and transformed host cells for the production of enterococcal aminoacyl-tRNA synthetases. They can also be used in the further isolation of nucleic acids related by DNA sequence similarities, which also encode enterococcal aminoacyl-tRNA synthetases, or portions thereof. A further embodiment of the invention is antisense nucleic acid which can hybridize to the nucleic acid which encodes the aminoacyl-tRNA synthetase of enterococci. The invention also relates to tRNA synthetases such as isolated and/or recombinant enterococcal aminoacyl-tRNA synthetases. Antibodies which bind to these enzymes can be made and can be used in the purification and study of the enzymes.
    Type: Grant
    Filed: May 14, 1997
    Date of Patent: April 24, 2001
    Assignee: Cubist Pharmaceuticals, Inc.
    Inventors: Jianshi Tao, Mandana Sassanfar, Paul L. Gallant, Xiaoyu Shen, Anthony S. Avruch, Russell V. Yu, Shamila Nair