Abstract: A sample-to-answer microfluidic system and method including vertical microfluidic device and automated actuation mechanisms, such as, but not limited to, automated mechanical and/or magnetic actuation, is disclosed. In some embodiments, the sample-to-answer microfluidic system includes a vertically oriented microfluidic device and a rotating actuator in relation to the microfluidic device, and wherein the microfluidic device and the rotating actuator are operating in the XZ plane. Additionally, methods of using the sample-to-answer microfluidic system are provided.

Abstract: The present invention provides methods and devices for detecting and quantifying multiple biomolecules at single-molecule level using an integrated droplet microfluidic system. In one embodiment, the present invention provides real-time and digital measurement of multiple biomolecules in a sample, thereby quantifying multiple biomolecules in an absolute and simultaneous manner. In one embodiment, the present invention provides a diagnostic method for a disease, comprising real-time and digital measurement of multiple biomolecules in a sample using the method or device described herein.

Abstract: A method for analyzing a biological fluid specimen. The collection cartridge is inserted into a reader. The plurality of reactive elements of the collection cartridge following insertion of the collection cartridge is optically read recurrently. The first color change of at least one reactive element of the collection cartridge is detected and dated following the recurrent optical reading. The biological fluid specimen from the collection cartridge is analyzed following the detection of the color change of at least one reactive element of the collection cartridge.

Abstract: An electrochromic device comprising a substrate, a set of electrodes disposed on or within the substrate, and a layer comprising ?-WO3 disposed in electrical communication with the set of electrodes, wherein the layer of ?-WO3 exhibits polarization switching are described. Methods of making and using the electrochromic devices are also described. The electrochromic devices are used for detecting acetone in a fluid. The observed change in color of the ?-WO3 layer can be correlated with a subject's medical condition, such as diabetes.

Abstract: Microfluidic devices are provided for separating particulates that have a major dimension above a predetermined threshold value from a fluid, the device comprising an inlet, an inlet channel, a curved channel, a separation chamber, a first outlet and a second outlet; the inlet being connected to the inlet channel, the inlet channel is connected to the curved channel, the curved channel is connected to the separation chamber and the separation chamber is connected to the first outlet by a first outlet channel, and the separation chamber is connected to the second outlet by a second outlet channel; the first outlet channel comprises a serpentine portion; wherein the second outlet channel branches from the separation chamber substantially perpendicular to the first outlet channel.

Abstract: A liquid holding assembly, comprising a first tube and a second tube. The first tube has a first longitudinal wall extending between a closed first bottom end and a first top end. The second tube is configured to be inserted in the first tube, and a second longitudinal wall extending between a closed second bottom end and a second top end, the second bottom end having a perforation and comprising a holding unit configured to hold a sponge below the second bottom end. The second tube is configured to be inserted into the first tube to compress the sponge between the first bottom end and the second bottom end, to cause the sponge to release a liquid absorbed therein. A tight fit between the first longitudinal wall and the second bottom end is configured to force the released liquid to travel into the second tube via the perforation.

Abstract: The present invention relates to a microfluidic chip for sorting living cells. A sample flow channel communicates with a liquid inlet end of a sorting flow channel. A gas inlet flow channel, a target flow channel and a non-target flow channel communicate with a liquid outlet end of the sorting flow channel. An included angle between the target flow channel and the sorting flow channel is from 100° to 130°, and an included angle between the non-target flow channel and the sorting flow channel is from 100° to 140°. A distance between an intersection of an axis of the gas inlet flow channel and an axis of the sorting flow channel and an intersection of the sorting flow channel, the target flow channel and the non-target flow channel is from 0.02 mm to 0.05 mm.

Abstract: Variable temperature analytical assemblies are provided and/or methods for changing temperatures of a mass are provided. Low thermal conductance components and/or assemblies are also provided. Methods for thermally isolating a mass from a support structure are also provided.

Abstract: The object of the present invention is a microfluidic method for cleaning and/or regenerating at least one microfluidic sensor comprising at least one glass microchannel forming a microfluidic circuit and at least two electrodes, comprising at least the following steps of cleaning the microfluidic circuit, comprising at least the circulation of a fluid sample in the microchannel; and step of cleaning the microfluidic circuit, comprising at least the circulation of a nitric acid solution in the microchannel.

Abstract: Provided herein are compositions, systems, kits, and methods for performing an apoA1 exchange assay to determine the risk of MACE and/or for conducting an action based on the assay results. In some embodiments, methods are provided for generating, or receiving, a report that graphically displays: i) the subjects ApoA1 exchange rate in a sample as lower than a control or threshold value, and ii) the subjects risk of a major adverse cardiac event (MACE) and/or cardiovascular disease as being higher than normal.

Abstract: A lab-on-a-chip cartridge includes a housing defining four separate chambers. A fluid (such as whole blood) flows through one of the chambers and into another one of the chambers, which includes a filter membrane. The filter membrane is rotated to separate a first fluid component (such as plasma) from a second fluid component (such as red blood cells), with the first fluid component passing through the filter membrane and the second fluid component not passing through the filter membrane. The separated first and second fluid components each flow into a different one of the remaining chambers, with the first fluid component contacting a lab-on-a-chip device for analyzing the first fluid component.

Abstract: Systems, devices, kits, and methods are provided herein in the form of example embodiments that relate to calibration of medical devices. The medical devices can be sensors adapted to sense a biochemical attribute. The embodiments can be used to determine calibration information specific to an individual medical device. The embodiments can determine the calibration information by reference to one or more parameters obtained during manufacturing of the medical device. The embodiments can also determine the calibration information by reference to in vitro testing of the medical devices. The embodiments also apply to systems incorporating those medical devices in their use in the field. Also described are embodiments of modifications to surfaces of sensor substrates, such as through applied radiation and/or the creation of a well, to aid in the placement and/or sizing of a sensor element on the substrate.

Abstract: Systems, devices, kits, and methods are provided herein in the form of example embodiments that relate to calibration of medical devices. The medical devices can be sensors adapted to sense a biochemical attribute. The embodiments can be used to determine calibration information specific to an individual medical device. The embodiments can determine the calibration information by reference to one or more parameters obtained during manufacturing of the medical device. The embodiments can also determine the calibration information by reference to in vitro testing of the medical devices. The embodiments also apply to systems incorporating those medical devices in their use in the field. Also described are embodiments of modifications to surfaces of sensor substrates, such as through applied radiation and/or the creation of a well, to aid in the placement and/or sizing of a sensor element on the substrate.

Abstract: Systems, devices, kits, and methods are provided herein in the form of example embodiments that relate to calibration of medical devices. The medical devices can be sensors adapted to sense a biochemical attribute. The embodiments can be used to determine calibration information specific to an individual medical device. The embodiments can determine the calibration information by reference to one or more parameters obtained during manufacturing of the medical device. The embodiments can also determine the calibration information by reference to in vitro testing of the medical devices. The embodiments also apply to systems incorporating those medical devices in their use in the field. Also described are embodiments of modifications to surfaces of sensor substrates, such as through applied radiation and/or the creation of a well, to aid in the placement and/or sizing of a sensor element on the substrate.

Abstract: Formation potential for disinfection by-products (DBPs) is determined in-situ using one or more automated sample extraction and measurement mechanisms. In one embodiment, such an in-situ mechanism is used to take periodic water samples and measure trihalomethane (THM) concentration in near real-time (i.e., less than two hours), using a measurement process based on modified Fujiwara chemistry. During the extraction and measurement process, water samples can be heated according to a specific temperature/time profile in order to artificially accelerate age of the water sample, so as to cause DBPs to form prematurely. A water monitoring network can monitor detected DBP levels and take automated response actions according to predefined computer policies or rules.

Abstract: The storage system is intended to provide users a device, that may securely hold biological samples within a cell in a space saving storage assembly. It is further an aim of the storage system to enable easy retrieval of the biological samples through a simple two-step retrieval action of the cell. Furthermore, the system includes a compact storage assembly that comprises multiple cells and canisters stacked together having efficient structural components that are suited for a typical freezer tank for biological samples. Additionally, the storage system comprises a simple elevator system that enables easy and fast retrieval of a single canister and cell from a large group of canisters.

Abstract: Embodiments disclosed herein may relate to a testing apparatus. The testing apparatus may include a sealed, chemically-resistant testing apparatus body defining a testing void; a first fluid port and a second fluid port; and a first geomaterial and a second geomaterial. The first and second geomaterials may be positioned between the first fluid port and the second fluid port and relative to one another such that a flow channel may be provided between the first and second geomaterials. The first and second geomaterials may be coupled to a testing void interior surface so as to restrict flow to the flow channel between an upflow region and a downflow region of the testing void. The first and second geomaterials may be comprised of a natural formation material.

Abstract: The disclosure relates to pH sensitive color indicators which indicate the efficacy of a cleaning composition for antimicrobial activity by exhibiting a color change. In particular, the pH sensitive color indicators comprise a pH sensitive dye and a substrate, wherein the dye is bound to the substrate. In an aspect of the disclosure, the dye can be chemically bound, physically bound, coated on a substrate, or painted on a substrate.

Abstract: According to embodiments of the present application, a color change sensor comprises a color change indicator and a substrate comprising a gas disposed on the color change indicator. The sensor may further comprise an interface layer that assists in containing the gas within the substrate. The sensor may be used with limited use, restricted use, or disposable apparatuses. The user may actuate the sensor by disrupting or removing the gas-containing substrate and/or the interface layer. Methods of making and using the color change sensor and related apparatuses are also disclosed.

Abstract: An atomic level deposition for mass functionalization of a cavity filled with a pathogen sensitive antibody reagent to functionalize each biosensor using atomic level vapor phase deposition enables high volume production of this sensor technology. A biosensor has a first substrate and a second substrate with a cavity formed in the first substrate to form a membrane. Holes are formed through the second substrate. An aluminum oxide layer is formed over the cavity and into the holes to form cores. The cavity is filled with a pathogen sensitive antibody reagent. A biofluid sample with the pathogen is deposited over the membrane. The biofluid is drawn through the cores to mix with the antibody reagent. The antibodies combine with the pathogen to change the impedance along the current path. The presence of the pathogen changes the ionic current flow through the biosensor for a positive detection of the pathogen.