Abstract: Disclosed are an expression cassette for isopropanol production, a recombinant vector for isopropanol production expression cassette, a including the recombinant microorganism for isopropanol production into which the vector is introduced, and a method of producing isopropanol using the recombinant microorganism. The recombinant microorganism in which a succinic acid bypass metabolic pathway is introduced to an isopropanol production pathway has very high ability to produce isopropanol. The recombinant microorganism is capable of producing isopropanol in an amount corresponding to about 100 times the maximum amount of isopropanol that is produced using known Corynebacterium glutamicum, and thus can effectively produce isopropanol and can be useful in various industrial fields where isopropanol is utilized.

Abstract: The present invention falls within the technical field of aquaculture, and specifically, the invention relates to a specific solution for preventing and treating bacterial diseases using a Lactococcus lactis lactic acid bacterium transformed to produce an interferon type II (IFN II) immunostimulating cytokine, particularly interferon gamma (IFNg or IFNy). Said transformed bacterium has been deposited in the Chilean Microbial Genetic Resources Collection at INIA with accession number RGM 2416 dated 22 Oct. 2017.

Abstract: The object is to provide a method for inducing pili formation in bacterium of genus Bifidobacterium and a method for promoting intestinal colonization of the bacterium. 3-Phenylpropionic acid and/or 3-(4-hydroxyphenyl)propionic acid are an active ingredient of a composition for inducing the pili formation in the bacterium of genus Bifidobacterium and a composition for promoting the intestinal colonization of the bacterium.

Abstract: A method of producing chemicals includes providing fermentative cells; co-feeding any of galacturonate and galacturonate polymers with carbohydrates to the fermentative cells; and producing a chemical from the fermentative cells. The fermentative cells may include any of Clostridium acetobutylicum and Clostridium saccharoperbutylacetonicum. The carbohydrates may include any of glucose, mannose, galactose, fructose, arabinose, xylose, sucrose, lactose, maltose, cellobiose, and starch. The method may include providing a substantially equal proportion of the any of galacturonate and galacturonate polymers and the carbohydrates for co-feeding to the fermentative cells. The method may include altering a proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The method may include modulating a production of the chemical by altering the proportion of the any of galacturonate and galacturonate polymers to the carbohydrates. The chemical may include any of acetate and butyrate.

Abstract: The present invention provides engineered leucine decarboxylase (LDC) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered leucine decarboxylase (LDC) polypeptides. In some embodiments, the engineered LDC polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis, and/or increased tolerance to low pH environments. In some embodiments, the engineered LDC polypeptides are optimized to provide improved storage stability. The present invention also provides methods for the use of the compositions comprising the engineered LDC polypeptides for therapeutic and industrial purposes.

Abstract: The present invention discloses Stenotrophomonas maltophilia GYH and its application in degrading chlorinated hydrocarbon pollutants, and the application is carried out as follows: resting cells obtained by spreading cultivation of Stenotrophomonas maltophilia GYH are added to a pH=5-8 inorganic salt medium, and then a chlorinated hydrocarbon pollutant is added, and the cells are cultured at 20-35° C. and 140-180 rpm to degrade the pollutant. The concentration of chlorinated hydrocarbon pollutant which can be removed by Stenotrophomonas maltophilia GYH ranges from 2.9 mg/L to 8.93 mg/L. Therefore, the Stenotrophomonas maltophilia has a highly efficient degradation ability for similar industrial pollutants and is able to withstand high concentrations of these pollutants.

Abstract: An isolated bacterial strain of Lactiplantibacillus plantarum subsp. plantarum MFM 30-3 deposited under the DSMZ Accession No. DSM 34213 is provided; a probiotic composition including an Lactiplantibacillus plantarum subsp. plantarum MFM 30-3 and optionally, one or more additional probiotic organisms that enhance the probiotic activity of the Lactiplantibacillus plantarum subsp. plantarum MFM 30-3 is also provided; and a method for preventing or treating chronic kidney disease in a subject in need thereof including: administering to the subject a pharmaceutically effective amount of the probiotic composition including an isolated bacterial strain of Lactiplantibacillus plantarum subsp. plantarum MFM 30-3, and optionally, one or more additional probiotic organisms that enhance the probiotic activity of the Lactiplantibacillus plantarum subsp. plantarum MFM 30-3 is further provided.

Abstract: An Exiguobacterium indicum YAN2 having high-concentration selenite tolerance, the preservation number thereof being GDMCC No. 61594, and a method for fermenting inorganic selenium into nano-selenium by using the strain are provided. The obtained nano-selenium can promote the growth of leaf vegetables, increase the content of chlorophyll, Vc, and total phenol, etc, reduce the content of nitrate, and improve the quality of leaf vegetables. In addition, the present application further provides a nano-selenium liquid fertilizer prepared by fermentation using the strain.

Abstract: Disclosed herein is a yeast strain capable of fermenting high maltose syrups into ethanol in the presence of glucose. In commercially important fuel ethanol yeast strains, the presence of glucose prevents robust maltose fermentation. This invention causes enhanced co-fermentation of maltose and glucose in strains otherwise identical to commercial fuel ethanol yeast strains.

Abstract: The disclosure discloses a method for producing maltodextrin with a single polymerization degree by multienzyme coupling, and belongs to the technical field of biology. The disclosure provides a method for producing non-reducing maltodextrin with a uniform and low polymerization degree. After a reaction is preformed for 2-6 hours by using the method of the disclosure, the content of 4-O-?-maltohexaosyl ?-D-glucoside in a reaction solution can be as high as 57.2% to 77.3%, accounting for 50% to 90% of the total amount of maltodextrin in the reaction solution. In maltodextrin prepared by using the method of the disclosure, non-reducing maltodextrin with low polymerization degree only includes 4-O-?-maltohexaosyl ?-D-glucoside, and the content of non-reducing maltodextrin with low polymerization degree can be 50% to 90% of the total amount of maltodextrin.

Abstract: The present disclosure relates to a method of digesting a sample comprising protein, the method comprising: adding the sample to an apparatus containing a buffer and a solid support surface comprising a surface coating, wherein the surface coating immobilizes enzyme while reducing undesired interactions between the sample and the solid support surface; immobilizing enzymes on the surface coating for digestion of the protein of the sample; and heating the sample to complete a heat-assisted digestion of the protein.

Abstract: The present invention relates to methods of treating gastrointestinal irregularity, as well as promoting, maintaining, and restoring gastrointestinal regularity, and maintaining healthy gut microflora in an individual. The present invention relates to methods comprising orally administering to an individual a Bacillus subtilis composition wherein the individual's gastrointestinal irregularity is treated, and/or the individual's gastrointestinal regularity and/or healthy gut microflora is promoted, maintained, and/or restored.

Abstract: Disclosed herein is a composition for modulating immunity, comprising Lactobacillus paracasei LT12, ?-glucan, and Bovine Colostrum Powder. Also provided is a method for modulating immunity, comprising administering to a subject in need a therapeutically effective amount of the composition, wherein the method is to enhance the expression of IL-6, IL-10, TNF-? and TGF-?1.

Abstract: Provided is a novel technique for reducing damage and death of microbial cells through a method for producing highly viable dried microbial cells, the method being characterized by subjecting dried microbial cells to an alternating temperature treatment.

Abstract: A medium composition for detection of P. carotovorum includes pectin, cellobiose, and inositol as active ingredients. P. carotovorum is highly likely to cause soft rot during cultivation as well as storage and transportation such that continuous monitoring is required. In order to solve the issues, the medium composition ensures remarkably outstanding selectivity for P. carotovorum.

Abstract: It is an object of an aspect of the present invention to provide (i) transformants, whose proliferation depends on phosphite, of various species of organism and (ii) a method for detecting the presence of a reduced phosphorous compound with use of such a transformant. Use is made of a transformant which is defective in functions of a gene encoding a phosphate transporter protein and a gene encoding a phosphate ester transporter protein and into which a gene encoding a hypophosphite transporter protein is introduced, a signal peptide of a hypophosphite binding protein being substituted with a signal peptide derived from a host or a species of organism closely related to the host.

Abstract: Cyclic GMP-AMP synthase (cGAS) enzymes have been engineered to produce polypeptides having increased cGAS activity in the cyclization of modified nucleoside triphosphates, including thiolated or fluorinated nucleoside triphosphates, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing cGAS enzymes, compositions comprising the cGAS enzymes, and methods of using the engineered cGAS enzymes are useful for the production of pharmaceutical compounds.

Abstract: The present specification discloses methods and uses for treating a migraine disorder. The disclosed method comprising extramuscularly administering a Botulinum toxin to an individual in one of more areas of a fronto-fascial layer located in a frontal head region of the individual, one of more areas of a temporoparietal-fascial layer located in a temporal head region of the individual, and one or more areas of an occipito-fascial layer located in an occipital head region of the individual. The disclosed methods and uses further comprise extramuscularly administering a Botulinum toxin to one or more nerve exit points and/or one or more subcutaneous locations in the head and neck as well as one or more sites of an epicranial aponeurosis. The disclosed methods and uses further comprise intramuscularly administering a Botulinum toxin to one or more locations within the left and right Splenius Capitus muscle, the left and right Masseter muscles, the left and right Trapezius muscles, or any combination thereof.

Abstract: Disclosed herein is a method for assessing the probability of embryo implantation success during the in vitro fertilization process. A method of the disclosure can predict embryo implantation success based on, for example, the amount of specific metabolites present in embryo conditioned culture media.

Abstract: The present invention relates to a lipase-producing strain and application thereof. The strain is classified and named Bacillus subtilis CS1802, with a preservation number of CCTCC NO: M2018262. The strain can be used to produce vitamin A palmitate through whole-cell transformation of vitamin A and palmitic acid. The Bacillus subtilis CS1802 of the present invention is derived from traditional natural fermented food and is a microorganism generally recognized as safe. The strain can be easily cultured and preserved. The highest content of vitamin A palmitate obtained through whole-cell transformation of vitamin A and palmitic acid is 15.35 mg/L. The highest transformation efficiency is 76.75%. The strain provides a new path for enzymatic synthesis of vitamin A palmitate and has important application prospects.