Abstract: [Problem] To provide a glucose dehydrogenase with high thermal stability to be used in blood sugar measurement. [Solution to Problem to problem] A modified glucose dehydrogenase with an excellent property of high thermal stability that can be obtained by modifying some of amino acids constituting a protein such as a wild-type glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor, and methods for manufacturing the measuring reagent composition and the biosensor.

Abstract: A dehydrated composition containing a particle encapsulating one or more microorganisms is provided. The composition is useful for controlling the release of the microorganisms following rehydration and propagation within the particle.

Abstract: A process for producing butanol is provided, involving: A) mixing water, lactate, an enzyme mixture comprising at least one enzyme, at least one cofactor and at least one coenzyme, to prepare a reaction mixture; B) catalytically reacting the reaction mixture for an amount of time sufficient to cause conversion of lactate into butanol; and wherein the conversion of lactate into butanol in B) is associated with a regeneration system of NAD (P)+/NAD (P) H and/or acetyl-CoA/CoA.

Abstract: Disclosed herein is an isolated strain of Lactobacillus acidophilus TW01, which is deposited at Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH under an accession number DSM 33990. Also disclosed herein are uses of the isolated strain of Lactobacillus acidophilus TW01 for alleviating an inflammation-related disorder and for improving gut health.

Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to methods of making a structure including nanotubes, a structure including nanotubes, methods of delivering a fluid to a cell, methods of removing a fluid to a cell, methods of accessing intracellular space, and the like.

Abstract: A composition for the treatment of atopic dermatitis, the composition including, as an active ingredient, Bifidobacterium animalis subsp. lactis LM1017, and more specifically provided is a composition for the treatment of atopic dermatitis. The composition includes, as an active ingredient, a navel Bifidobacterium animalis subsp. lactis LM1017 which adjusts the NF-?B signal transduction pathway, and thus inhibits the expression of proinflammatory cytokines.

Abstract: An approach for modifying multiple types of bacteria to produce surface modifications that enhance the immunologic response when used as a vaccine. A series of plasmids (pYF, pYFC, pYFP, pSF, pSPF, and pSCF) may be used to transform bacteria which then produce surface-exposed ligands that bind to complement receptors on antigen presenting cells. When modified bacteria are used as a vaccine, the vaccine recipients produce significantly higher titers of specific antibodies and are better protected against challenges from the disease-causing bacteria.

Abstract: The present invention provides compositions, methods, and kits related to reverse transcriptases derived from E.r. maturase.

Abstract: A mutant algal strain showing upregulation of mRNA transcripts encoding urea carboxylase, ?-15-?3-desaturase and downregulation of mRNA transcripts of gene encoding triacylglycerol lipase is provided herein. The mutant algal strain of the present disclosure is tolerant to low temperature and thus can be grown over a wide temperature range. The strain shows enhanced biomass and fatty acid production and enhanced growth rate and nitrogen metabolism over a wide temperature range of about 10° C. to about 37° C., wherein the enhancement is in comparison to the wild type algal strain. A method of obtaining the mutant algal strain and a method of producing industrially relevant products such as fatty acids from the mutant algal strain also are provided herein.

Abstract: The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition.

Abstract: Covalently cross-linked pilus polymers displayed on the cell surface of Gram-positive bacteria are assembled by class C sortase enzymes. These pilus-specific transpeptidases located on the bacterial membrane catalyze a two-step protein ligation reaction—first, cleaving the LPXTG motif of one pilin protomer to form an acyl-enzyme intermediate, and second, joining the terminal threonine to the nucleophilic lysine residue residing within the pilin motif of another pilin protomer. Informed by the high-resolution crystal structures of corynebacterial pilus-specific sortase (SrtA) and by developing structural variants of the sortase enzyme whose catalytic pocket has been unmasked by activating mutations, we have developed new reagents capable of forming isopeptide bonds in vitro. The reagents disclosed herein can catalyze ligation of isolated SpaA domains in vitro provide a facile and versatile new platform for protein engineering and bio-conjugation that has major implications for biotechnology.

Abstract: An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.

Abstract: A liquid sporulation method is described. A liquid culture is prepared by adding bacterial cells to a sporulation broth. The liquid culture is incubated. Spores are harvested from the incubated liquid culture by separating the spores from a culture supernatant. In some embodiments, the harvested spores are suspended in an aqueous medium to form a spore suspension. At least a portion of the culture supernatant is combined with the spore suspension.

Abstract: A Geomyces mutant strain and an application thereof, relating to the technical field of the screening and application of functional microorganisms; the mutant strain is obtained through a mutagenic method, capable of greatly improving the yield of red pigment; the mutant strain has been preserved in the China Center for Type Culture Collection of Wuhan University on Jan. 24, 2019, and the preservation number is CCTCC NO: M2019086; the mutant strain can be widely used in the field of natural pigment production, can reduce the production cost of red pigment, and has a bright application prospect.

Abstract: The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

Abstract: The present disclosure relates to the fields of biotechnology, molecular biology and genetic engineering. In particular, the present disclosure relates to a genetically modified alga comprising a recombinant cytochrome c6 gene, methods of producing the same and applications thereof. The present disclosure also relates to a codon optimised nucleic acid sequence encoding a cytochrome c6 polypeptide, expression cassette, vectors and host cell thereof. In an embodiment, the present disclosure also relates to a method of increasing biomass and photosynthetic efficiency of algae.

Abstract: A medium material for removing phenol contamination from groundwater, a method of producing the same, and use of the same id disclosed. In at least some embodiments, the medium material is a granular material which has an average particle diameter of 0.5-1.5 cm and is formed from a bacteria-entrapping solution, a manganese sand filter material, modified bentonite, and biochar at a mass ratio of 1:0.2-0.4:0.2-0.4:0.1-0.2 by a series of processes including strain culturing, catalysis, mixing, solidification, and the like. The medium material can remove phenol from groundwater, is a safe and environment-friendly material, has a long service life, and/or achieves waste treatment with waste.

Abstract: A method of screening/prognosis/diagnosis for colorectal cancer (CRC) wherein methionine aminopeptidase 2 (MetAP2) levels are detected in a non-tumor sample such as peripheral blood, peripheral blood mononuclear cells (PBMC) or lymphocytes. Based on the MetAP2 levels, the individual may be selected for further testing.

Abstract: The purpose of the present invention is to provide a detection means whereby Staphylococcus aureus can be identified at a high accuracy. An aspect of the present invention relates to a medium for detecting S. aureus which comprises one or more kinds of nutrient components, a color developing agent capable of developing a color in the presence of ?-glucosidase, a color developing agent capable of developing a color in the presence of phosphatase and 0.5 mg/cm3 or more of sodium colistin methanesulfonate. Another aspect of the present invention relates to a sheet for detecting S. aureus, said sheet comprising the aforesaid medium, and a method for detecting S. aureus with the use of the medium and sheet as described above.

Abstract: Disclosed herein are medical products, including an implantable device coated with a crosslinked extracellular matrix comprising at least one of Type IV collagen and laminin, wherein the crosslinked extracellular matrix contains no more than 0.024 mg/ml total concentration of glucose, amino acids and salts having a molecular weight of 2000 daltons or less. Corresponding systems and method also are disclosed.