Abstract: Provided is a device for amplifying and detecting a gene. The device for amplifying and detecting the gene includes a gene amplification chip comprising channels through which a sample flows and transparent heaters provided on the gene amplification chip. The channels include a first channel, a second channel, and a third channel, and the first to third channels have a triangular loop structure, and the transparent heaters include a first transparent heater, a second transparent heater, and a third transparent heater, which are respectively provided on the first to third channels.

Abstract: In some embodiments, the systems and methods of the disclosure can provide high-throughput, programmable, and fully automatized tissue and/or cell culture and analysis platforms. In some embodiments, a culture analysis system may include a culture device that includes a cover configured to be secured to a main body, which may include one or more chambers. The cover may include one or more regions that overlaps with the one or more chambers of the main body when the cover is secured to a main body so that each region corresponds to a chamber of the main body. The cover may also include a fluidic pathway disposed in each region and configured be in fluidic communication with a corresponding chamber. Each fluidic pathway may include a fluid inlet and a fluid outlet disposed in each region. The cover may also include an optical pathway disposed in each region for the corresponding chamber.

Abstract: A tray configured to be provided on a base station of a bioreactor system, said tray comprising a bottom and surrounding side walls and being configured to hold a bioreactor bag, said bottom comprising at least two bottom parts, whereof one is a middle part of the bottom, wherein at least one of the bottom parts is movable into at least a first and a second position, wherein said first position provides the bottom parts substantially flush with each other providing a substantially plane bottom of the tray and said second position provides a bottom of the tray having a recessed middle part.

Abstract: A storage container includes: a substrate having a plurality of storage wells to store an object therein, the storage wells being formed on a predetermined substrate surface of the substrate. Further, the storage wells each includes an opening forming portion forming an opening portion opening on the substrate surface, and having an inclined surface that inclines from the substrate surface downward in a depth direction on an inner surface of the opening portion; and a bottomed storage portion that has a side wall surface extending in a direction perpendicular to the substrate surface on a lower side in the depth direction of the storage well relative to the opening forming portion, and that communicates with a region on the substrate surface through the opening portion, and a lower end of the inclined surface is connected to the side wall surface of the storage portion, forming a ridgeline.

Abstract: When a stirring device 11 performs processing of stirring a mixed solution in a test tube S, a holding mechanism 13 and a movement mechanism 14 are operated so that the test tube S is moved to a position of B2. Then, in the test tube S, the cells attached to the inner surface of the test tube S and the liquid are separated. Further, the cells attached to the inner surface of the test tube S are detected by a color sensor 152. Therefore, it is possible to reduce the work for determining the end of the stirring processing by the user. As a result, the workability of the user when using the stirring device 11 can be improved.

Abstract: A portable incubator system integrated to a mobile phone providing a real-time tracking of samples and data flow is provided. The portable incubator system allowing cells to be cultured, reproduced and characterized in real-time without a need for a commercial incubator and a microscope-camera system installed within the portable incubator system.

Abstract: A culture additive diffusion mechanism (23) includes an additive retention unit (20) configured to retain an additive (21) used in a culture and a diffusion adjustment unit (22) configured to adjust a diffusion rate of the additive (21) from the inside of the additive retention unit (20) to the outside of the additive retention unit (20).

Abstract: A microalgae curtain wall includes photobioreactors, an interior glass panel, an exterior glass panel, and transoms. The photobioreactors are adapted to receive sunlight and carbon dioxide to grow microalgae received therein. The exterior glass panel is offset from the interior glass panel forming a gap therebetween. The transoms hold the interior glass panel and the exterior glass panel therebetween. The transoms suspend the photobioreactors in the gap and between the interior glass panel and the exterior glass panel.

Abstract: An intelligent bacteria collection system includes a bottle opening module and a peristaltic pump module opposite to each other, wherein a guide rail is arranged between the bottle opening module and the peristaltic pump module, and a tray module is placed on the guide rail; a manipulator module and the peristaltic pump module are fixed on an infusion operation platform; a control infusion module is placed on the tray module, which can be moved to and fixed on the infusion operation platform by the manipulator module. The present invention replaces the conventional artificial bacteria collection process, makes the bacteria collection process more rapid, reduces the possible secondary pollution in the manual detection operation process, improves the accuracy of the bacteria collection detection, and improves the work efficiency. The present invention provides high degree of automation, wherein no manual operation is required in the bacteria collection process.

Abstract: Disclosed herein are various bioreactor devices and systems for growing cellular material, and related methods of growing cellular material. In some cases, a system can include a well plate having a plurality of wells and a bioreactor situated in each well of the well plate. In some cases, a bioreactor can include an inner body which divides the bioreactor into several distinct chambers and facilitates the growth of a multi-tissue sample in the bioreactor. In some cases, a system can include a mechanical actuator situated to mechanically stress tissues grown in a bioreactor.

Abstract: This disclosure relates to apparatus and methods for thermally cycling a sample. Particular embodiments comprise a first pivot arm configured to pivot around a first pivot axis; a second pivot arm configured to pivot around a second pivot axis; a first thermal mass and a second thermal mass coupled to the first pivot arm; and a third thermal mass and a fourth thermal mass coupled to the second pivot arm, wherein the first and third thermal masses are proximal to the sample when the first and second pivot arms are in a first position, and the second and fourth thermal masses are proximal to the sample when the first and second pivot arms are in a second position.

Abstract: In an aspect, there is provided a computer-implemented method for approximating product production in a bioreactor. The method comprises: providing a bioreactor containing live cells in a substrate, the bioreactor for cultivating, over a time period, a product derived from or of the cells during a manufacturing process; providing two or more sensors for measuring respective two or more different physical attributes of the substrate during the time period; receiving, at a processor, sensor data from the two or more sensors; and determining via the processor, based on a predetermined or recursive algorithm that correlates the sensor data and the product production by independent consideration of the sensor data of the two or more different physical attributes or by multivariate consideration of the sensor data of the two or more different physical attributes, an approximate amount of the product.

Abstract: The invention relates to an incubator, a system and a method for the monitoring of the growth of cells in a cell culture by acquiring cell monitoring data in the incubator. The incubator comprises a data storage device (4a) and a data processing device (4b) that are configured to acquire at least one incubator chamber value and at least one cell growth value in form of cell monitoring data and to store the cell monitoring data in the data storage device.

Abstract: Provided is a charged particle beam apparatus capable of more objectively and highly accurately calculating a feature of a cell from an observation image of a cell and evaluating a cell. The charged particle beam apparatus includes an image acquisition unit 18 that acquires an image of a cell, a contour extraction unit 19 that extracts a contour of the image, a feature calculation unit 20 that calculates a morphological feature of the contour based on the contour and calculates the feature of an internal structure such as a cytoplasm contained in an internal area of the contour, and a determination unit 21 that determines quality and/or functionality of the cell based on the feature, and can objectively and highly accurately evaluate the quality and/or the functionality of the cell included in the captured image.

Abstract: The disclosure provides for electrode systems and perfusion systems that may be configured to measure the electrical activity of an explanted heart and to provide defibrillation energy as necessary. The perfusion systems may maintain the heart in a beating state at, or near, normal physiological conditions; circulate oxygenated, nutrient enriched perfusion fluid to the heart at or near physiological temperature, pressure, and/or flow rate. These systems may include a pair of electrodes that may be placed epicardially on the right atrium and/or left ventricle of the explanted heart, and/or an electrode placed in the aortic blood path.

Abstract: A cell culture apparatus may include a substrate defining a well. The well may define an interior surface, an exterior surface, an upper aperture and a nadir. The substrate may define a thickness between the interior and exterior surfaces that has a thickness proximate the nadir that is greater than or equal to a thickness proximate the upper aperture.

Abstract: A device for culturing specimens may include a housing and a conveyor disposed within the housing, where the conveyor is translatable in vertical directions. The device may further include a plurality of plate holders disposed along a length of the conveyor, such that each plate holder of the plurality of plate holders can be moved to different positions along the length of the conveyor by a translation of the conveyor in the vertical directions. The device may further include an isolation chamber movable between a first position and a second position. The isolation chamber may include an opening and a door. The isolation chamber may be configured such that when the isolation chamber is in the second position, a first plate holder of the plurality of plate holders is isolated from a rest of the housing by the isolation chamber.

Abstract: A mold capable of preparing a cellular or acellular construct includes a block including a three-dimensional cavity for a sample, a base, and a removable sheet inserted between the block and the base. The sheet may be coated with silicone grease prior to insertion of the sheet between the block and the base. Mechanical constraints may be included at each end of the cavity to constrain cell driven contraction and to allow for gripping the sample after the sample is removed from the mold. The sample may be prepared by partially filling the cavity with gel, filling the remaining portion of the cavity with culture media, and allowing a three-dimensional sample to culture within the cavity for a time. The sample may be transported to a bioreactor and ejected from the mold by removing the sheet and applying pressure to the mechanical constraints.

Abstract: Systems, methods, and apparatuses are provided for self-contained nucleic acid preparation, amplification, and analysis.

Abstract: A sample is introduced from a bioreactor to a chamber included in a manifold. The chamber includes at least a first probe. The at least the first probe is coupled to a controller. One or more measurements are performed on the sample while the sample is located in the chamber for a measurement period. The sample is returned from the chamber to the bioreactor after the one or more measurements of the sample have been performed.