Patents Examined by Lydia Edwards
  • Patent number: 10093963
    Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols.
    Type: Grant
    Filed: March 22, 2017
    Date of Patent: October 9, 2018
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Michael T. Kusner, Jeffrey Williams
  • Patent number: 10086374
    Abstract: Disclosed is a multi-stage thermal convection apparatus and uses thereof. In one embodiment, the invention features a three-stage thermal convection apparatus that includes a temperature shaping element for assisting a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: October 2, 2018
    Assignee: Ahram Biosystems, Inc.
    Inventor: Hyun Jin Hwang
  • Patent number: 10086375
    Abstract: Disclosed is a multi-stage thermal convection apparatus such as a two-stage thermal convection apparatus and uses thereof. In one embodiment, the two-stage thermal convection apparatus includes a temperature shaping element that assists a thermal convection mediated Polymerase Chain Reaction (PCR). The invention has a wide variety of applications including amplifying nucleic acid without cumbersome and expensive hardware associated with many prior devices. In a typical embodiment, the apparatus can fit in the palm of a user's hand for use as a portable, simple to operate, and low cost PCR amplification device.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: October 2, 2018
    Assignee: Ahram Biosystems, Inc.
    Inventor: Hyun Jin Hwang
  • Patent number: 10087409
    Abstract: Provided is a cell culturing vessel for holding and culturing cells. It has a first vessel that stores a culture medium and cells or only a culture medium, a second vessel that is placed above the first vessel and stores a culture medium and cells or only a culture medium, a main vessel that holds the first vessel and houses the second vessel, and a lid member that engages with the main vessel. The main vessel has a pressing member that fixes and holds the first vessel in the main vessel and the second vessel is eccentrically held in the first vessel by the pressing member.
    Type: Grant
    Filed: September 26, 2012
    Date of Patent: October 2, 2018
    Assignee: Hitachi, Ltd.
    Inventors: Masaharu Kiyama, Guangbin Zhou, Ryota Nakajima, Takayuki Nozaki, Naoko Senda, Shizu Matsuoka
  • Patent number: 10081788
    Abstract: A product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump.
    Type: Grant
    Filed: September 19, 2016
    Date of Patent: September 25, 2018
    Assignee: Repligen Corporation
    Inventor: Jerry Shevitz
  • Patent number: 10071403
    Abstract: A system and method for decomposing municipal solid waste (MSW) under anaerobic conditions. The system includes a bioreactor landfill with the MSW, a leachate recirculation unit in connection with the bioreactor landfill, a multilevel thermocouple unit, placed inside the bioreactor landfill for monitoring temperature in the bioreactor landfill. Further, the system includes a sensor for monitoring moisture level within the bioreactor landfill. Furthermore, the system includes the leachate recirculation unit configured to initiate recirculation of a leachate, collected from the bioreactor landfill, when temperature in the bioreactor reaches a determined temperature and a determined moisture level.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: September 11, 2018
    Assignee: Indian Institute of Technology Bombay
    Inventors: Devendra Narain Singh, Bhagawan Shamrao Patil
  • Patent number: 10066198
    Abstract: A device for in-vitro modelling in-vivo tissues of organs that includes a first body portion with at least one access chamber, a second body portion with at least one culturing chamber, and a culturing membrane dividing the at least one access chamber from the culturing chamber. The device further includes a third body portion with at least one actuation chamber having at least one limitation cavity, and an actuation membrane dividing the at least one culturing chamber from the at least one actuation chamber. With the device, a robust actuation system can be provided that does not depend on the mechanical properties of the actuation membrane material, nor on the pressure, and that allows to mimic three-dimensional deformations of the tissue, in particular of lung alveoli.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: September 4, 2018
    Assignee: Universität Bern
    Inventors: Olivier Thierry Guenat, Marcel Felder, Andreas Stucki, Janick Daniel Stucki, Thomas Geiser
  • Patent number: 10058869
    Abstract: A micro-fluidic device includes a plurality of heating elements on a substrate for heating the substrate. They define a plurality of temperature regions having distinct temperatures on the substrate. A flow feature layer is formed above the substrate to define a channel extending across the substrate through each temperature region. As fluid is repeatedly pumped within the channel, it flows from one temperature region to a next temperature region to undergo thermal cycling. Fluid wells reside in the flow feature layer under a cover and connect to the channel whereby fluid can dwell for diagnostic applications.
    Type: Grant
    Filed: March 21, 2016
    Date of Patent: August 28, 2018
    Assignee: Microfluidic MEMS Solution, LLC
    Inventors: Steven Wayne Bergstedt, Stephen Jacob DeMoor, Yimin Guan
  • Patent number: 10047331
    Abstract: A tray (100; 400; 500; 600) for accommodating a cell culture (101), such as an embryo, for use during culturing thereof and for optical monitoring of the cell culture, e.g. during in vitro fertilization comprises a carrier structure (104;404;504;604) defining at least one accommodating zone (102; 402; 502; 602) for accommodating the cell culture. At least one focal lens (110; 410; 510; 610), notably a numerical aperture increasing lens is integrally formed with the carrier structure to facilitate monitoring of the cell culture through the carrier structure. A diameter of the focal lens may exceed a diameter of the at least one accommodating zone. The focal lens may be integrally molded with the carrier structure from a thermoplastic material.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: August 14, 2018
    Assignee: UNISENSE FERTILITECH A/S
    Inventors: Jonas Lerche Hansen, Niels B. Ramsing, Søren Porsgaard, Holger Søe Plougsgaard, Mai Faurschou Isaksen
  • Patent number: 10041062
    Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.
    Type: Grant
    Filed: April 21, 2016
    Date of Patent: August 7, 2018
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Jeffrey Williams, Sundaresh Brahmasandra, Michael T. Kusner
  • Patent number: 10023833
    Abstract: A temperature sensor means comprising a thermally conducting layer arranged to face the surface to be measured, a thermally insulating layer attached to the thermally conducting layer on the opposite side to the surface to be measured, a temperature sensor provided between the thermally conducting layer and the thermally insulating layer and being completely surrounded on all its sides by either the thermally conducting or the thermally insulating layers.
    Type: Grant
    Filed: June 16, 2014
    Date of Patent: July 17, 2018
    Assignee: GE HEALTHCARE BIO-SCIENCES AB
    Inventors: Patrik Akerstrom, Hakan Wahlnas
  • Patent number: 10010888
    Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a capture plate and a capture plate module configured to facilitate binding of nucleic acids within the set of biological samples to magnetic beads; a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols; and an assay strip configured to combine nucleic acid samples with molecular diagnostic reagents for analysis of nucleic acids.
    Type: Grant
    Filed: January 24, 2017
    Date of Patent: July 3, 2018
    Assignee: NeuMoDx Molecular, Inc.
    Inventors: Jeffrey Williams, Sundaresh Brahmasandra, Michael T. Kusner
  • Patent number: 10006861
    Abstract: An improved device and system for facilitating polymerase chain reaction including a light source, detector, waveguide, and filters that occupy minimal space and facilitate detection of stationary samples, reduced sample read time, and simultaneous reading of multiple light wavelengths.
    Type: Grant
    Filed: June 30, 2014
    Date of Patent: June 26, 2018
    Assignee: Streck, Inc.
    Inventors: Matthew R. Kreifels, Scott E. Whitney, Gregg Wilder, John Flanagan, James Dowling, Philip Hills, Michael Tilleman, Jeremie Jackson
  • Patent number: 9993782
    Abstract: A bioreactor has a stirrer shaft that is driveable by a drive and on which a stirrer element is positioned. The position of the stirrer element is determined by a stirrer installation aid. The stirrer installation aid has a free end with an abutment surface on which the free end of the stirrer element abuts. The stirrer installation aid is designed as a sleeve that comprises a slot extending between the axial ends of the sleeve. The slot is delimited by opposite flanks of a wall, and the sleeve is placed on the stirrer shaft. The stirrer installation aid can be pulled off laterally from the stirrer shaft. A method for installing the stirrer element on the stirrer shaft arranged in the bioreactor also is provided.
    Type: Grant
    Filed: August 29, 2013
    Date of Patent: June 12, 2018
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Thomas Dreher, Oliver Scheibe, Gerhard Greller, Ute Husemann, Oscar-Werner Reif
  • Patent number: 9994807
    Abstract: A temperature sensor means comprising a thermally conducting layer arranged to face the surface to be measured, a thermally insulating layer attached to the thermally conducting layer on the opposite side to the surface to be measured, a temperature sensor provided between the thermally conducting layer and the thermally insulating layer and being completely surrounded on all its sides by either the thermally conducting or the thermally insulating layers.
    Type: Grant
    Filed: June 16, 2014
    Date of Patent: June 12, 2018
    Assignee: GE HEALTHCARE BIO-SCIENCES AB
    Inventors: Patrik Akerstrom, Hakan Wahlnas
  • Patent number: 9994806
    Abstract: A transport device for a container has a base, a vertical member extending up from the base and a complementary handling unit extending from an end of the vertical member spaced from the base. The complementary handling unit is configured for releasably engaging a container, such as a bioreactor container, so that the container can be transported to a holding device.
    Type: Grant
    Filed: November 8, 2016
    Date of Patent: June 12, 2018
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Bernward Husemann, Wolfgang Kahlert, Sebastien Chaussin, Reiner Seitz
  • Patent number: 9994808
    Abstract: The present invention is directed to compositions, tools, methods and devices to culture microorganisms and, in particular, to compositions, tools, methods and devices for the detection of microorganisms in biological samples.
    Type: Grant
    Filed: August 11, 2016
    Date of Patent: June 12, 2018
    Assignee: Vax-Immune, LLC
    Inventors: Bhairavi Parikh, James Stone
  • Patent number: 9983197
    Abstract: Low-cost and portable microfluidic systems are needed for cell migration research and Point of Care (POC) testing. This study introduces a low-cost and portable USB Microscope Microfluidic Chemotaxis Analysis System (UMCAS) for rapid analysis of cell chemotaxis studies. A standalone microfluidic gradient generator is also developed for rapid generation of chemical gradient in microfluidic device without need of any peripheral perfusion apparatus. A smart phone based application program was developed for the real-time remote monitoring of the migration data. This system is validated by observing the neutrophil migration in three different conditions: 1) medium control, 2) uniform IL-8 control, and 3) IL-8 gradient. The results show that neutrophils exhibit random migration in both medium and uniform IL-8 control experiments, while they show strong directional migration to the IL-8 gradient. These results successfully validated the developed UMCAS system.
    Type: Grant
    Filed: September 10, 2013
    Date of Patent: May 29, 2018
    Inventors: Francis Lin, Jiandong Wu
  • Patent number: 9970922
    Abstract: With the object of preventing deterioration of or damage to a photodetector caused by excessive light by more reliably preventing the excessive light from entering the photodetector, a microscope of the present invention is provided with a high-sensitivity detector, such as an HPD, a GaAsP, an EM-CCD or the like, that detects observation light coming from a specimen, a box-shaped casing that has an opening that allows contained items to be placed therein and removed therefrom and that covers the high-sensitivity detector, a door that can close off the opening of the casing, a switch that restricts light detection by the high-sensitivity detector by turning on and off a drive voltage to be applied to the high-sensitivity detector, and an opening restricting mechanism that allows the opening of the casing in the closed state imposed by the door to be opened only when the light detection by the high-sensitivity detector is restricted by the switch.
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: May 15, 2018
    Assignee: OLYMPUS CORPORATION
    Inventors: Atsuhiro Tsuchiya, Hiroyuki Okahira, Koichiro Izumi
  • Patent number: 9963668
    Abstract: The invention relates to a device for culturing cells in a bioreactor, the waste-air line of which leading out of the reactor interior comprises a waste-air filter having a sterile-filtration microfilter membrane, wherein at least one prefilter having a hydrophobic filter material is placed upstream of the waste-air filter toward the reactor interior.
    Type: Grant
    Filed: July 16, 2013
    Date of Patent: May 8, 2018
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Oscar-Werner Reif, Gerhard Greller, Paschalis Nikoloudis, Ralf Lausch, Ute Husemann, Thomas Loewe, Thomas Friese, Thomas Dreher