Abstract: Mixtures of monoclonal antibodies which contain effective assaying amounts of each of at least two monoclonal antibodies that bind to different antigenic sites on the antigen and are capable under appropriate conditions of binding simultaneously to an antigen are useful in enhanced sensitivity assays for the antigen. By utilizing such mixtures in diagnostic assays for important antigens such as the polypeptide human chorionic gonadotropin enhanced sensitivity can be achieved as compared with assays employing individual monoclonal antibodies.

Abstract: The present invention relates to a process for the manufacture of a preparation containing specific antibodies against the K-99 antigen of various strains of enterotoxigenic Escherichia coli (etec). In said process the milk obtained from postpartum cows, which had been previously vaccinated with E. coli strain 168, is clotted with a suitable enzyme. The fatty serum is then separated from the cheese obtained, the fat is then separated out, the fat-free serum is then subjected to ultrafiltration through a suitable number of appropriate membranes so that 30-50% of the water present is removed the retentate is then sterilized. Suitable membranes are those which are permeable only to molecules of less than about 22,000-30,000 daltons molecular weight. The preparation is advantageously lyophilized and the lyophilized preparation is subsequently sterilized by irridation, e.g. with Co.sup.60.

Abstract: Human monoclonal antibodies which specifically bind to antigens found on cell surfaces of renal, lung, and breast cancer cells, intracellular cytoskeletal antigens, nuclear antigens, and cytoplasmic reticular antigens are disclosed. The antibodies are the product of hybridoma cell lines, where the immortal cell line may be, e.g., a human cell line, or a murine cell line.

Abstract: There is disclosed a process for preparing anti-NMB-1 from Mallory bodies wherein Mallory bodies are removed and isolated from a liver and Mallory antibodies are produced by hybridoma techniques followed by isolation of the anti-NMB-1 having two polypeptide components of 45 and 66 kd by Western blotting.

Abstract: A murine monoclonal antibody combining site produced by a hybridoma formed by fusion of cells from a myeloma cell line and lymphocytes that produce antibodies that react (1) with isolated human C3b receptor and (2) with C3b receptor-bearing cells from a mammal immunized with human C3b receptor is disclosed.

Abstract: Processes and compositions are provided for in vitro use in tissue culture media (preferred), perfusion media, and incubation media. Thus, balanced salt solutions are provided wherein the milliequivalent ratio of sodium cations to chloride anions is normalized and wherein optionally at least one near-equilibrium couple is incorporated (bicarbonate.sup.- /carbon dioxide, L-lactate.sup.- /pyruvate.sup.-, and d-betahydroxybutyrate.sup.- /acetoacetate.sup.-. Regulation of intracellular and extracellular pH, and normalization of intracellular redox state and phosphorylation state is achievable.

Abstract: A method of distinguishing subsets within a plurality of human cells including producing a monoclonal antibody to a non-human primate cell, contacting the monoclonal antibody with the human cells, and distinguishing the subsets on the basis of different degrees of reactivity with the monoclonal antibody.

Abstract: A process for the production of radioiodinated spiperone and certain derivatives thereof, and novel compositions made by the process. The process includes reacting spiperone, thallium trichloride and radioiodine in a sealed reaction vial. The novel compounds are useful for diagnosis of disease states resulting in quantitative changes in dopaminergic receptors.

Abstract: This invention provides a process for single cell protein production which involves the culturing of Methylophilus methylotrophus microorganisms by facultative growth on glucose as a carbon and energy source.

Abstract: A method is provided for solid phase immunoassay for quantitation of antigen, hapten or antibody analyte in liquid sample and alternatively for quantitation of analyte occuring on or attached to cells or other particulate material in a liquid sample. The solid phase is suspended for at least the initial reactions and is subsequently concentrated by microfiltration to a volume substantially less than the sample volume. Luminescence of substantially the entire label on the concentrated solid phase is measured.

Abstract: Process for disrupting cells of a polysaccharide-producing microorganism of the genus Pseudomonas, preferably Pseudomonas sp. NCIB 11592, which contain one or more RP4 and/or RP4::Mu.sub.ts plasmids.Pseudomonas sp. NCIB 11592 containing one or more plasmids. Process for preparing polysaccharides by cultivating the latter. Process for displacing a fluid through a well and/or a permeable subsurface formation using the polysaccharides.

Abstract: The invention relates to a novel hybridoma continuous cell line that produces a monoclonal antibody of the IgG type to the glycoprotein GP-2, a subunit of the basement membrane component laminin. The hybridoma cell line is formed from a spleen cell from a Lewis rat, which has been immunized with a basement membrane antigen, fused with a mouse myeloma cell.

Abstract: Fluorescence ligand binding assay of a sample containing an unknown amount of ligand may be performed by making direct intensity measurements. In an immunoassay, for example, the sample may be added to a solution containing fluorescein labeled analyte and then is added an antibody specific to the analyte. Sodium dodecyl sulfate, a surfactant, added to the solution in an amount sufficient to form micelles provides markedly different fluorescent intensity from bound and unbound labeled analyte.

Abstract: Monoclonal anti-ornithine decarboxylase antibody is produced by cell hybrids between hypoxanithine phosphoriboxyltransferase deficient myeloma cells and spleen cells derived from an animal previously immunized with ornithine decarboxylase. By means of immunoaffinity chromatography using cyanogen bromide-activated agarose coupled with the antibody, ornithine decarboxylase in animal cell extracts can be highly purified in high yield.

Abstract: Monoclonal antibodies specific for thyroxine (T.sub.4) are produced by two new and separate hybridoma cell lines. Combinations of the monoclonal antibodies from the two cell lines are used in an immunoassay for T.sub.4 of high accuracy over the range of T.sub.4 concentrations encountered in serum samples.

Abstract: Monoclonal antibodies have been developed that bind with one or more proteins located on the surface of gametes or zygotes of malaria parasites. These antibodies are specific for antigens on mosquito midgut stages of malaria parasite and sterilize the parasites in mosquitoes otherwise capable of transmitting the disease. The monoclonal antibodies are specific for the 255, 59 and 53 kilodalton surface proteins on Plasmodium falciparum and for the 25 kilodalton surface protein on zygotes and ookinetes of Plasmodium gallinaceum.

Abstract: An hybridoma cell line and the monoclonal antibody produced thereby are provided. The monoclonal antibody is specific for antigen found almost exclusively in the cytoplasm of human mammary tumor cells. The antibody is useful in diagnosing mammary tumors.

Abstract: Collagen profiles of human body tissues and fluids, i.e., the types of distinct connective tissue proteins present, their distribution in human body tissues and fluids, and the concentration ratios among distinct types, are subject to change during certain pathological conditions and during therapeutic regimens for the treatment of such conditions. These changes in collagen profiles can be detected by immunohistological, immunocytological and immunoserological techniques. In vitro diagnostic methods employing monoclonal antibodies specific for connective tissue proteins are provided which can be used for monitoring the results of therapeutic measures taken against inflammatory diseases, fibrotic diseases and cancer and for detecting or following the pathogenesis of such diseases.

Abstract: A process for determining the presence of polyvalent antigens by incubation with three receptors is presented. In addition, a kit for carrying out this process is provided as well. One of said receptors is bound to a solid support and the other two, in solution, derive from the same animal species.

Abstract: Liability in human individuals to develop non-insulin-dependent diabetes mellitus (NIDDM) and/or atherosclerosis is determined by restriction enzyme mapping of DNA from a human individual using a probe selected from the group consisting of(i) cDNA complementary to the mRNA coding for the human insulin,(ii) human genomic DNA containing the actual insulin gene,(iii) DNA sequences of human genomic DNA located within 20.times.10.sup.6 base pairs from the insulin gene in either direction,and examining the distribution of DNA fragments for the appearance of insertion sequences of approximately 1600 to 2300 base pairs (U alleles) the occurrence of which indicates a liability for said individual to develop elevated blood glucose concentrations and/or atherosclerosis and in homozygous form a liability to develop NIDDM.