Patents Examined by Margaret Moskowitz
  • Patent number: 5563256
    Abstract: Unique species-specific Eimeria tenella DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria tenella.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: October 8, 1996
    Assignee: Merck & Co., Inc.
    Inventors: Prasanta R. Chakraborty, Michael Dashkevicz, Alex Elbrecht, Scott D. Feighner, Paul A. Liberator, Helen Profous-Juchelka
  • Patent number: 5493009
    Abstract: The invention concerns murine antiidiotypic monoclonal antibodies which are the internal image of determinants recognized by a monoclonal antibody on high molecular weight-melanoma associated antigen (HMW-MAA), antibody derivatives, hybridoma cell lines secreting such antiidiotypic monoclonal antibodies, and processes for the preparation of such antiidiotypic monoclonal antibodies, of their derivatives and of the hybridoma cell lines. The murine antiidiotypic monoclonal antibodies are useful for the determination of antibodies directed against high molecular weight-melanoma associated antigen, for the modulation of the immune response to HMW-MAA and for the treatment of melanoma.
    Type: Grant
    Filed: April 2, 1993
    Date of Patent: February 20, 1996
    Assignee: New York Medical College
    Inventor: Soldano Ferrone
  • Patent number: 5482845
    Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
    Type: Grant
    Filed: September 24, 1993
    Date of Patent: January 9, 1996
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Marcelo B. Soares, Argiris Efstratiadis
  • Patent number: 5474770
    Abstract: The present invention relates to a biological support for cell cultures formed by the coagulated mixture of a concentrate of plasma proteins and thrombin.The protein concentrate is obtained by precipitating fresh plasma with ethanol and contains balanced proportions of fibrinogen, Factor XIII and fibronectin. The thrombin concentration is adjusted to obtain the desired consistency of the support coagulated in the form of a film.The biological support is preferably used for preparing a culture of keratinocytes, recovering them in the form of a reconstituted tissue and transporting same. The reconstituted tissue is thus particularly suitable for use as a graft.
    Type: Grant
    Filed: March 12, 1992
    Date of Patent: December 12, 1995
    Assignee: Centre Regional de Transfusion Sanguine de Lille
    Inventors: Herve Broly, Vincent Ronfard
  • Patent number: 5449768
    Abstract: Unique species-specific Eimeria praecox DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria praecox.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: September 12, 1995
    Assignee: Merck and Co., Inc.
    Inventors: Prasanta R. Chakraborty, Michael Dashkevicz, Alex Elbrecht, Scott D. Feighner, Paul A. Liberator, Helen Profous-Juchelka
  • Patent number: 5420264
    Abstract: The present invention relates, in general, to substantially pure polynucleotide molecules specifying chimpanzee or rhesus monkey CD4, and fragments thereof and Gp120 binding molecules related to human CD4. The present invention further relates to polynucleotide molecules specifying CD4 fusion proteins and host cells containing the polynucleotide molecules.
    Type: Grant
    Filed: July 17, 1992
    Date of Patent: May 30, 1995
    Assignee: The General Hospital Corporation
    Inventors: Brian Seed, David Camerini
  • Patent number: 5359050
    Abstract: Unique species-specific Eimeria mitis DNA probes comprising divergent DNA sequences are disclosed. The probes ere complementary to a small subunit ribosomal RNA gene of Eimeria mitis.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: October 25, 1994
    Assignee: Merck and Co., Inc.
    Inventors: Prasanta R. Chakraborty, Alex Elbrecht, Michael Dashkevicz, Scott D. Feighner, Paul A. Liberator, Helen Profous-Juchelka
  • Patent number: 5349058
    Abstract: Nucleic acid sequences, particularly DNA sequences, coding for all or a portion of human mevalonate kinase, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods for detecting the DNA sequences or the corresponding RNA sequences. The invention also concerns polypeptide molecules comprising all or a portion of human mevalonate kinase.
    Type: Grant
    Filed: February 6, 1990
    Date of Patent: September 20, 1994
    Assignee: E. R. Squibb & Sons, Inc.
    Inventors: Richard D. Tanaka, Beverly S. Ricci, Stephen T. Mosley
  • Patent number: 5298613
    Abstract: Unique species-specific Eimeria acervulina DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria acervulina.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: March 29, 1994
    Assignee: Merck and Co., Inc.
    Inventors: Prasanta R. Chakraborty, Alex Elbrecht, Michael Dashkevicz, Scott D. Feighner, Paul A. Liberator, Helen Profous-Juchelka
  • Patent number: 5298430
    Abstract: Immunoassay process for the detection of an antigen in a sample of blood, serum, urine and other liquids employing a test apparatus comprising a reaction chamber and a support element in the reaction chamber comprising a blend of from about 5 to 95 percent cellulose organic ester fibrets and from about 95 to 5 percent by weight of a dispersible cut fibers where a predetermined amount of an antibody capable of extracting an antigen from the sample is bound to the support element, the process comprising depositing the sample on the upper surface of the support element and detecting the amount of antigen in the sample.
    Type: Grant
    Filed: September 13, 1988
    Date of Patent: March 29, 1994
    Assignee: Hoechst Celanese Corporation
    Inventors: Thomas J. Myers, Jr., Lessie C. Phillips
  • Patent number: 5288845
    Abstract: Unique species-specific Eimeria necatrix DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria necatrix.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: February 22, 1994
    Assignee: Merck and Co., Inc.
    Inventors: Prasanta R. Chakraborty, Michael Dashkevicz, Alex Elbrecht, Scott D. Feighner, Paul A. Liberator, Helen Profous-Juchelka
  • Patent number: 5283173
    Abstract: Methods are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.
    Type: Grant
    Filed: January 24, 1990
    Date of Patent: February 1, 1994
    Assignee: The Research Foundation of State University of New York
    Inventors: Stanley Fields, Ok-Kyu Song
  • Patent number: 5278298
    Abstract: Unique species-specific Eimeria brunetti DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria brunetti.
    Type: Grant
    Filed: May 12, 1992
    Date of Patent: January 11, 1994
    Assignee: Merck & Co., Inc.
    Inventors: Prasanta R. Chakraborty, Alex Elbrecht, Michael Dashkevicz, Scott D. Feighner, Paul A. Liberator, Helen Profous-Juchelka
  • Patent number: 5276146
    Abstract: Liquid perfluorocarbon supports useful as liquid affinity supports are provided. The support is based on an inert perfluorocarbon carrier with ligands or binders for the ligands attached to its surface through a highly fluorinated isocyanate anchor group.
    Type: Grant
    Filed: September 17, 1991
    Date of Patent: January 4, 1994
    Assignee: E. I. Du Pont de Nemours and Company
    Inventors: Julian P. Breillatt, Jr., John W. D. Eveleigh
  • Patent number: 5273882
    Abstract: A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.
    Type: Grant
    Filed: November 20, 1991
    Date of Patent: December 28, 1993
    Assignees: Amgen, Abbott Laboratories
    Inventors: David L. Snitman, Stephen D. Stroupe
  • Patent number: 5273878
    Abstract: The present invention relates to nucleic aid molecules which comprise subfragments of ABR gene sequence. In particular embodiments, the nucleic acid molecules of the invention comprise portions of nucleic acid sequence contained in plasmids pVNTR-A or pVNTR-B. The invention is based, in part, on the discovery that a Taq-1 fragment of pVNTR-B, an EcoRI/HindIII fragment of pVNTR-A and, in preferred embodiments, a combination of these two fragments may be used to demonstrate restriction fragment length polymorphisms in the DNA of human subjects. Such restriction fragment length polymorphisms may provide a "genetic fingerprint" which may be used to identify individual persons or to provide evidence of a filial relationship in paternity cases. The nucleic acid sequences of the invention offer the advantage of producing an easily readable pattern in restriction fragment polymorphism analysis.
    Type: Grant
    Filed: August 30, 1990
    Date of Patent: December 28, 1993
    Inventors: John Groffen, Nora Heisterkamp
  • Patent number: 5272057
    Abstract: The invention relates to a method for detecting the presence of a DNA polymorphism associated with predisposition for certain cancers in a human. This method involves analyzing the human chromosome 13 using a hybridization probe which will hybridize to the gene for human poly (ADP-ribose) polymerase wherein the probe is capable of identifying the DNA polymorphism.
    Type: Grant
    Filed: October 14, 1988
    Date of Patent: December 21, 1993
    Assignee: Georgetown University
    Inventors: Mark E. Smulson, Kishor Bhatia, Konrad Huppi
  • Patent number: 5270447
    Abstract: A family of metalloproteinases exist which cleave extracellular matrix molecules. These metalloproteinases are secreted in a latent inactive form and require activation in order to specifically cleave the preferred substrate. A series of peptides have been prepared based on the complete sequence analysis of type IV Procollagenase. Peptide inhibitors were synthesized which correspond to cysteine repeat regions and histidine containing regions; the mechanism of action of these peptides involves inhibition of binding of the enzyme to the substrate. Peptide inhibitors were synthesized which correspond to the peptide cleaved off during activation, and constitute a novel class of metalloproteinase inhibitors. These inhibitors are members of a series of peptides which contain the core amino acid sequence PRCG. The cysteine residue is required for activity.
    Type: Grant
    Filed: March 1, 1989
    Date of Patent: December 14, 1993
    Assignee: The United States of America as represented by the Department of Health & Human Services
    Inventors: Lance A. Liotta, William Stetler-Stevenson, Henry Krutzsch
  • Patent number: 5270178
    Abstract: A method for the recombinant production of zymogen forms of human protein C is described. These zymogen forms differ from native zymogen protein C in their increased sensitivity to activation by thrombin and thrombin/thrombomodulin. DNA compounds, vectors, and transformants useful in the method are also disclosed.
    Type: Grant
    Filed: February 23, 1990
    Date of Patent: December 14, 1993
    Assignee: Eli Lilly and Company
    Inventors: Bruce E. Gerlitz, Brian W. Grinnell
  • Patent number: 5270162
    Abstract: An autoradiographic gene-screening method employing a hybridization process, which comprises:(1) a step of transferring at least a portion of nucleic acids, fragments thereof or derivatives thereof resolved on a medium onto a transfer support to fix them thereonto;(2) a step of hybridizing the nucleic acids, fragments thereof or derivatives thereof fixed onto said transfer support with radioactively labeled probes; and(3) a step of obtaining locational information on the radioactively labeled substances on said transfer support, which comprises placing said transfer support having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on said transfer support, exciting said stimulable phosphor sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission.
    Type: Grant
    Filed: July 29, 1991
    Date of Patent: December 14, 1993
    Assignee: Fuji Photo Film Co., Ltd.
    Inventors: Hisashi Shiraishi, Junji Miyahara, Hisatoyo Kato