Abstract: Unique species-specific Eimeria tenella DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria tenella.

Abstract: The invention concerns murine antiidiotypic monoclonal antibodies which are the internal image of determinants recognized by a monoclonal antibody on high molecular weight-melanoma associated antigen (HMW-MAA), antibody derivatives, hybridoma cell lines secreting such antiidiotypic monoclonal antibodies, and processes for the preparation of such antiidiotypic monoclonal antibodies, of their derivatives and of the hybridoma cell lines. The murine antiidiotypic monoclonal antibodies are useful for the determination of antibodies directed against high molecular weight-melanoma associated antigen, for the modulation of the immune response to HMW-MAA and for the treatment of melanoma.

Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.

Abstract: The present invention relates to a biological support for cell cultures formed by the coagulated mixture of a concentrate of plasma proteins and thrombin.The protein concentrate is obtained by precipitating fresh plasma with ethanol and contains balanced proportions of fibrinogen, Factor XIII and fibronectin. The thrombin concentration is adjusted to obtain the desired consistency of the support coagulated in the form of a film.The biological support is preferably used for preparing a culture of keratinocytes, recovering them in the form of a reconstituted tissue and transporting same. The reconstituted tissue is thus particularly suitable for use as a graft.

Abstract: Unique species-specific Eimeria praecox DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria praecox.

Abstract: The present invention relates, in general, to substantially pure polynucleotide molecules specifying chimpanzee or rhesus monkey CD4, and fragments thereof and Gp120 binding molecules related to human CD4. The present invention further relates to polynucleotide molecules specifying CD4 fusion proteins and host cells containing the polynucleotide molecules.

Abstract: Unique species-specific Eimeria mitis DNA probes comprising divergent DNA sequences are disclosed. The probes ere complementary to a small subunit ribosomal RNA gene of Eimeria mitis.

Abstract: Nucleic acid sequences, particularly DNA sequences, coding for all or a portion of human mevalonate kinase, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods for detecting the DNA sequences or the corresponding RNA sequences. The invention also concerns polypeptide molecules comprising all or a portion of human mevalonate kinase.

Abstract: Unique species-specific Eimeria acervulina DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria acervulina.

Abstract: Immunoassay process for the detection of an antigen in a sample of blood, serum, urine and other liquids employing a test apparatus comprising a reaction chamber and a support element in the reaction chamber comprising a blend of from about 5 to 95 percent cellulose organic ester fibrets and from about 95 to 5 percent by weight of a dispersible cut fibers where a predetermined amount of an antibody capable of extracting an antigen from the sample is bound to the support element, the process comprising depositing the sample on the upper surface of the support element and detecting the amount of antigen in the sample.

Abstract: Unique species-specific Eimeria necatrix DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria necatrix.

Abstract: Methods are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.

Abstract: Unique species-specific Eimeria brunetti DNA probes comprising divergent DNA sequences are disclosed. The probes are complementary to a small subunit ribosomal RNA gene of Eimeria brunetti.

Abstract: Liquid perfluorocarbon supports useful as liquid affinity supports are provided. The support is based on an inert perfluorocarbon carrier with ligands or binders for the ligands attached to its surface through a highly fluorinated isocyanate anchor group.

Abstract: The present invention relates to nucleic aid molecules which comprise subfragments of ABR gene sequence. In particular embodiments, the nucleic acid molecules of the invention comprise portions of nucleic acid sequence contained in plasmids pVNTR-A or pVNTR-B. The invention is based, in part, on the discovery that a Taq-1 fragment of pVNTR-B, an EcoRI/HindIII fragment of pVNTR-A and, in preferred embodiments, a combination of these two fragments may be used to demonstrate restriction fragment length polymorphisms in the DNA of human subjects. Such restriction fragment length polymorphisms may provide a "genetic fingerprint" which may be used to identify individual persons or to provide evidence of a filial relationship in paternity cases. The nucleic acid sequences of the invention offer the advantage of producing an easily readable pattern in restriction fragment polymorphism analysis.

Abstract: A method and a kit for the isolation and quantitative detection of a selected target nucleic acid sequence from solution employing two probes. A first probe is complementary to one portion of the target and is covalently attached to a first complexing agent (e.g., either an antigen or an antibody). The second probe is complementary to a different portion of the target and is associated with a reporter group. Following hybridization of the target and two probes in solution, a solid support coated with a second complexing agent (i.e., a corresponding antibody or antigen) capable of binding to the first complexing agent on the first probe is employed to immobilize the target-probe hybrid complex. A plurality of types of first probes may be used. Each type is attached to the same sort of complexing agent but each includes a nucleic acid sequence which is complementary to a different portion of the target.

Abstract: The invention relates to a method for detecting the presence of a DNA polymorphism associated with predisposition for certain cancers in a human. This method involves analyzing the human chromosome 13 using a hybridization probe which will hybridize to the gene for human poly (ADP-ribose) polymerase wherein the probe is capable of identifying the DNA polymorphism.

Abstract: A method for the recombinant production of zymogen forms of human protein C is described. These zymogen forms differ from native zymogen protein C in their increased sensitivity to activation by thrombin and thrombin/thrombomodulin. DNA compounds, vectors, and transformants useful in the method are also disclosed.

Abstract: An autoradiographic gene-screening method employing a hybridization process, which comprises:(1) a step of transferring at least a portion of nucleic acids, fragments thereof or derivatives thereof resolved on a medium onto a transfer support to fix them thereonto;(2) a step of hybridizing the nucleic acids, fragments thereof or derivatives thereof fixed onto said transfer support with radioactively labeled probes; and(3) a step of obtaining locational information on the radioactively labeled substances on said transfer support, which comprises placing said transfer support having been subjected to the hybridization and a stimulable phosphor sheet in layers for a given period of time to cause said sheet to absorb at least a portion of radiation energy emitted by the radioactively labeled substances on said transfer support, exciting said stimulable phosphor sheet with an electromagnetic wave to release the radiation energy stored in said sheet as stimulated emission, and detecting the stimulated emission.

Abstract: A family of metalloproteinases exist which cleave extracellular matrix molecules. These metalloproteinases are secreted in a latent inactive form and require activation in order to specifically cleave the preferred substrate. A series of peptides have been prepared based on the complete sequence analysis of type IV Procollagenase. Peptide inhibitors were synthesized which correspond to cysteine repeat regions and histidine containing regions; the mechanism of action of these peptides involves inhibition of binding of the enzyme to the substrate. Peptide inhibitors were synthesized which correspond to the peptide cleaved off during activation, and constitute a novel class of metalloproteinase inhibitors. These inhibitors are members of a series of peptides which contain the core amino acid sequence PRCG. The cysteine residue is required for activity.