Patents Examined by Marian Knode
  • Patent number: 6004939
    Abstract: It was found that normal human stem cells produce a regulated non-processive telomerase activity, while cancer cells produce a processive telomerase activity. Nucleotide analogs, such as 7-deaza-2'-deoxyquanosine-5'-triphosphate (7-deaza-dGTP) were found to be substrates for processive telomerase and incorporated into telomeric sequence. The incorporation of this nucleotide subsequently affected the processivity of telomerase, converting processive telomerase to non-processive telomerase. The incorporation of this nucleotide analogs was also found to inhibit formation of G-quartets by telomeric sequence. Other methods for converting cancer processive telomerase to the more benign non-processive telomerase include partially cleaving the telomerase RNA.
    Type: Grant
    Filed: June 20, 1997
    Date of Patent: December 21, 1999
    Assignees: CTRC Research Foundation Board of Regents, The University of Texas System
    Inventors: Shih-Fong Chen, Ira Maine, Sean M. Kerwin, Terace M. Fletcher, Miquel Salazar, Blain Mamiya, Makoto Wajima, Bradford E. Windle
  • Patent number: 5874433
    Abstract: Inhibitor of the use of tetrahydrobiopterin as a cofactor for nitric oxide synthase, e.g., substituted 4-phenyl(hydropyridines) such as 1-methyl-4-(3',4'-dihydroxyphenyl)-1,2,3,6-tetrahydropyridine or, 4-(3',4'-dihydroxyphenyl)-1,2,3,6-tetrahydropyridine) or tetrahydropterin analog which does not replace tetrahydrobiopterin as a substrate for nitric oxide synthase such as (6R,S)-6,7-dimethyl-tetrahydropterin or (6R,S)-tetrahydrofolic acid or 2,4-diamino-, 2,6-diamino, or 4,6-diamino mono- or disubstituted pyrimidines or the corresponding pyrimidine-3-oxides such as 2,4-diamino-6-(diethylamino)pyrimidine, 2,4-diamino-6-piperidino-pyrimidine-3-oxide, 2,4-diamino-6-hydroxypyrimidine, 4,6-diamino-2-hydroxypyrimidine or 2,5-diamino-4,6-dihydroxypyrimidine is administered to inhibit nitric oxide synthesis from arginine in vascular cells in a subject in need of such inhibition (e.g., for prophylaxis or treatment of cytokine- or endotoxin-induced hypotension (e.g., septic shock).
    Type: Grant
    Filed: December 19, 1995
    Date of Patent: February 23, 1999
    Assignee: Cornell Research Foundation, Inc.
    Inventor: Steven S. Gross
  • Patent number: 5804551
    Abstract: A method for improving recovery after surgery or an invasive procedure is provided, including administering a hemoglobin preparation to a patient before surgery or an invasive procedure.
    Type: Grant
    Filed: November 12, 1996
    Date of Patent: September 8, 1998
    Assignee: Baxter International Inc.
    Inventor: Kenneth E. Burhop
  • Patent number: 5447859
    Abstract: Methods and compositions useful for the separation of viruses, including retroviruses and vital vectors, from preparations are disclosed. Sulfated oligosaccharides with at least about 6 .mu.moles sulfate per gram of oligosaccharide are provided. In one aspect, a sulfated oligosaccharide of the present invention may be used in the purification of a virus, such as a lipid envelope virus. The present invention also discloses methods for the removal of a contaminating virus from a preparation, such as biologic therapeutics.
    Type: Grant
    Filed: July 16, 1993
    Date of Patent: September 5, 1995
    Assignee: Viagene
    Inventor: Charles E. Prussak
  • Patent number: 5432079
    Abstract: Methods for the isolation of mutant rhizobial strains with improved competition of nodulation (Comp.sup.+) is presented. Mutants are selected for constitutive expression of inducible nod genes (in the absence of inducer) and screened for hyperinduction of nod genes in the presence of inducer. Mutants which are defective in nodulation or in symbiotic nitrogen fixation are eliminated from further testing. Selections for constitutive nod expression and for hyperinduction of nod genes are facilitated by the use of plasmid carrying a selectable marker fused to an inducible nod gene and a plasmid carrying a reporter gene fused downstream of an inducible nod gene. The methods exemplified are particularly useful for the isolation and identification of Comp.sup.+ mutants of Bradyrhizobium japonicum.
    Type: Grant
    Filed: March 11, 1988
    Date of Patent: July 11, 1995
    Assignee: Mycogen Plant Science, Inc.
    Inventors: Eric Johansen, Edward R. Appelbaum
  • Patent number: 5425943
    Abstract: An injection composition comprises a pharmacologically effective amount of a modified tPA, 47.5 mM or more of an amino acid or a salt thereof and is improved in view of stability and solubility.
    Type: Grant
    Filed: June 22, 1992
    Date of Patent: June 20, 1995
    Assignee: Eisai Co., Ltd.
    Inventors: Masahiro Kawahara, Yuichiro Hayashi, Naoki Asakawa, Sumio Watanabe, Yasuo Miyake
  • Patent number: 5422256
    Abstract: A strain of Bacillus subtilis which is a tryptophan auxotroph, resistant to sulfaguanidine and flourotryptophan and produces anthranilic acid in a nutrient solution containing L-tryptophan, and process for the preparation of anthranilic acid by fermentation.
    Type: Grant
    Filed: December 28, 1992
    Date of Patent: June 6, 1995
    Assignee: BASF Aktiengesellschaft
    Inventors: Bryan Cooper, Joachim Meyer, Klaus Euler
  • Patent number: 5409826
    Abstract: The invention describes the preparation of preserved, non-infectious control cells. The control cells are prepared by modification, activation or otherwise changing selected normal leukocyte cells, tissue culture cells or zenogenic transplants in immunosuppressed animals to have characteristics of abnormal cells or by the addition of cells from an established cell line, which has been similarly modified or activated, to a normal leukocyte population. The preferred method of preserving the control cells is by lyophilization using an isotonic 10% trehalose solution.
    Type: Grant
    Filed: June 8, 1993
    Date of Patent: April 25, 1995
    Assignee: Coulter Corporation
    Inventors: John A. Maples, Patricia A. Roth, Melissa J. Stiglitz
  • Patent number: 5409830
    Abstract: A novel E. coli strain which can produce L-phenylalanine and is resistant to high osmotic pressure and a process for producing L-phenylalanine by use of the novel E. coli (KCCM 10,016) are disclosed.
    Type: Grant
    Filed: June 21, 1994
    Date of Patent: April 25, 1995
    Assignee: Miwon Co. Ltd.
    Inventors: Byung L. Lim, Hong Rhym, Jin H. Lee, Tae Y. Choi, E. Nam Hwang, Hong K. Choi
  • Patent number: 5405756
    Abstract: A transparent storage stable acidic beverage or other alimentary product containing casein phosphopeptide is obtained by reacting casein with trypsin, precipitating insoluble components at acid pH, adding a divalent cation such as calcium to the reaction medium, recovering the reaction product, and adding the casein phosphopeptide to a mixture to produce a beverage or product having beneficial solubilizing effects on calcium.
    Type: Grant
    Filed: January 5, 1994
    Date of Patent: April 11, 1995
    Assignee: Meiji Seika Kaisha, Ltd.
    Inventors: Hiroshi Naito, Tadashi Noguchi, Ryuichiro Sato, Keiko Tsuji, Hidemasa Hidaka
  • Patent number: 5405761
    Abstract: Salts of L-ornithine are prepared by means of the enzymatic conversion of arginine to L-ornithine in the presence of the enzyme L-arginase in an aqueous medium in such a manner that the acid whose salt is to be prepared is used for the adjustment of the pH for the enzymatic conversion and for the subsequent neutralization of the reaction mixture and that the salt formed is isolated directly from the reaction mixture.
    Type: Grant
    Filed: June 17, 1991
    Date of Patent: April 11, 1995
    Assignee: Degussa Aktiengesellschaft
    Inventors: Kyriakos Makryaleas, Karlheinz Drauz
  • Patent number: 5403728
    Abstract: The invention refers to a new microbial process for preparing .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methy l-butyryloxy)-naphthalen-1-yl]-heptanoic acid .delta.-lactone of formula (I) and .beta.,.delta.-dihydroxy-7-[1,2,6,7,8,8a-hexahydro-2.6-dimethyl-8-(2-methy lbutyryloxy)-naphtalen-1-yl]-heptanoic acid of formula (II), ##STR1## by the aerobic fermentation of the submerged culture of an imperfect fungus strain biosynthesizing the above compounds, in a nutrient medium containing utilizable carbon and nitrogen sources as well as mineral salts, and by isolating the product of formula (I), which comprises cultivating a strain of the new Aspergillus obscurus fungus species producing the above compound(s) of formulas (I) and/or (II), in a temperature range of to C. and, if desired, separating the product(s) formed from the fermentation broth, then isolating it in the lactone form of formula (I) and, if desired, purifying the same.
    Type: Grant
    Filed: June 17, 1993
    Date of Patent: April 4, 1995
    Assignee: BIOGAL Gyogyszergyar RT
    Inventors: Antonia Jekkel, Eva Ilkoy, Istvan M. Szabo, Gabor Ambrus, Attila Andor, Ilona Varga, Imre Moravcsik, Istvan Szabo, Janos Erdei, Kalman Polya, Andras Kiss, Laszlo Cseke, Karoly Nagy, Mihaly Kaszas, Lajos Kiss, Istvan Magyi, Edit Halasz, Gyorgy Santha
  • Patent number: 5399492
    Abstract: Polyploid yeasts having an lti property are obtained by sporulating a starting polyploid strain of Saccharomyces cerevisiae, selecting at least one segregant having an lti property, backcrossing the selected segregant at least once with another segregant having an opposite mating type, selecting at least two backcross segregants having an lti property and opposite mating types, crossing the selected backcross segregants at least once, and selecting a polyploid strain having a growth potential, an lti property, and an ability to raise a dough.
    Type: Grant
    Filed: March 30, 1994
    Date of Patent: March 21, 1995
    Assignee: Nestec S.A.
    Inventors: Christof Gysler, Herbert Hottinger, Peter Niederberger
  • Patent number: 5393662
    Abstract: A chromogenic .beta.-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by .beta.-galactosidase and a chromogenic .beta.-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by .beta.-glucuronidase are combined in a test medium for quantitatively identifying and differentiating general coliforms and E. coli. The .beta.-galactosidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by .beta.-galactosidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-glucuronide, 4,6-dichloroindolyl-.beta.-D-glucuronide, 6,7-dichloroindolyl-.beta.-D-glucuronide, and 4,6,7-trichloroindolyl-.beta.-D-glucuronide, which produce mauve or magenta precipitates when reacted upon by .beta.-glucuronidase. The .beta.-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-.beta.
    Type: Grant
    Filed: March 1, 1993
    Date of Patent: February 28, 1995
    Assignee: RCR Scientific, Inc.
    Inventors: Jonathan N. Roth, Wilfred J. Ferguson
  • Patent number: 5391487
    Abstract: A restriction endonuclease is provided. The enzyme, designated SgfI, recognizes the DNA sequence ##STR1## and cleaves the DNA sequence at a position indicated by the arrows and is preferably obtainable from microorganisms of the genus Streptomyces.
    Type: Grant
    Filed: August 13, 1993
    Date of Patent: February 21, 1995
    Assignee: Promega Corporation
    Inventors: James R. Kappelman, Raymond J. Williams, Elizabeth E. Murray, Nadia I. Vesselinova
  • Patent number: 5391491
    Abstract: The invention relates to a biomass for producing virus/virus antigen, which consists of cell aggregates having diameters of between 100 .mu.m and 1,000 .mu.m. The biomass according to the invention has a high metabolic activity in suspension in the culture medium and is infected with virus. It enables the large-scale production of pure virus/virus antigen and is particularly suitable for the production of TBE-virus/virus antigen.
    Type: Grant
    Filed: July 6, 1992
    Date of Patent: February 21, 1995
    Assignee: Immuno Aktiengesellschaft
    Inventors: Wolfgang Mundt, Wilfried Woehrer, Friedrich Dorner, Johann Eibl
  • Patent number: 5391488
    Abstract: Biocatalytic oxidative processes wherein oxidizable substrates are reacted in the presence of a peroxidase such as soybean peroxidase or another legume peroxidase is described.
    Type: Grant
    Filed: April 19, 1993
    Date of Patent: February 21, 1995
    Assignee: Enzymol International, Inc.
    Inventors: Mark A. Johnson, Alexander R. Pokora, William L. Cyrus, Jr.
  • Patent number: 5389536
    Abstract: A substantially enzymatically pure hydrolase is provided which is secreted by and isolatable from Pseudomonas mendocina ATCC 53552. Cloning the gene expressing the hydrolase into a suitable expression vector and culturing, such as fermenting the E. coli strain JM101 harboring a plasmid designated pSNtacII, has been found to provide surprisingly high yields of the hydrolase.
    Type: Grant
    Filed: May 23, 1991
    Date of Patent: February 14, 1995
    Assignee: Genencor, Inc.
    Inventors: Gregory L. Gray, Scott D. Power, Ayrookaran J. Poulose
  • Patent number: 5382517
    Abstract: The invention relates to a process for preparing L-serine from glycine and formaldehyde in the presence of cells of a microorganism or a cell-treated product having a serine hydroxymethyl transferase activity by an enzymatic method.
    Type: Grant
    Filed: February 22, 1993
    Date of Patent: January 17, 1995
    Assignee: Mitsui Toatsu Chemicals, Inc.
    Inventors: Daisuke Ura, Tadashi Hashimukai, Toshio Matsumoto, Nobuhiro Fukuhara
  • Patent number: RE34837
    Abstract: By giving mammals, fowls, fish, etc. feeds containing Bacillus subtilis C-3102 FERM BP-1096), an excellent body weight gain and feed efficiency can be obtained.
    Type: Grant
    Filed: May 28, 1993
    Date of Patent: January 24, 1995
    Assignee: The Calpis Food Industry Co., Ltd
    Inventors: Takefumi Iwanami, Kiyoshi Maruta, Ichiya Murota, Hiroshi Miyazaki