Patents Examined by Maryam A. Monshipouri
  • Expression vector
    Patent number: 11390882
    Abstract: Disclosed herein are recombinant methods of activating expression of one or more biosynthetic gene clusters comprising more than one gene, the method comprising a recombinant DNA expression vector that possess two opposable inducible promoters that drives expression of a biosynthetic gene cluster exogenously from outside of the cluster to produce polyketides or non-ribosomal peptides in a heterologous host.
    Type: Grant
    Filed: March 11, 2020
    Date of Patent: July 19, 2022
    Assignee: VARIGEN BIOSCIENCES CORPORATION
    Inventors: Robert Joseph Stankey, David Mead
  • Method for producing inositol derivative
    Patent number: 11384372
    Abstract: A method for producing an inositol derivative includes a step of reacting inositol and dextrin in the presence of cyclodextrin glucanotransferase to generate an inositol derivative in which a sugar is bonded to the inositol, and to obtain a solution containing the inositol derivative and the cyclodextrin glucanotransferase; and a step of removing the cyclodextrin glucanotransferase in the solution using an ultrafiltration membrane, in which a deactivation treatment of the cyclodextrin glucanotransferase in the solution is not performed.
    Type: Grant
    Filed: September 4, 2018
    Date of Patent: July 12, 2022
    Assignee: SHOWA DENKO K.K.
    Inventors: Ichiro Fujita, Shinji Yamaki
  • Direct selection of cells expressing high levels of heteromeric proteins using glutamine synthetase intragenic complementation vectors
    Patent number: 11384140
    Abstract: This invention relates to the general field of recombinant expression of polypeptides in animal cell culture. More specifically, the invention concerns improved selection of cells transfected with recombinantly engineered vectors designed to express polypeptides, in particular heteromultimeric polypeptides.
    Type: Grant
    Filed: May 11, 2017
    Date of Patent: July 12, 2022
    Assignee: Amgen Inc.
    Inventors: Randal Robert Ketchem, Jeffrey T. McGrew, Dina A. Fomina Yadlin
  • Genetically encoded fluorescent sensors for detecting ligand bias and intracellular signaling through cAMP pathways
    Patent number: 11366114
    Abstract: Described herein are novel fluorescent sensors for cyclic adenosine monophosphate (cAMP) that are based on single fluorescent proteins. These sensors use less visible spectrum than FRET-based sensors, produce robust changes in fluorescence, and can be combined with one another, or with other sensors, in a multiplex assay on standard fluorescent plate readers or live cell imaging systems.
    Type: Grant
    Filed: November 4, 2014
    Date of Patent: June 21, 2022
    Assignee: Montana Molecular LLC
    Inventors: Thomas E. Hughes, Paul H. Tewson, Anne Marie Quinn
  • Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
    Patent number: 11365225
    Abstract: Methods of making recombinant secretion of silk and silk-amyloid proteins using bacteria are provided.
    Type: Grant
    Filed: November 18, 2016
    Date of Patent: June 21, 2022
    Inventors: Neel Satish Joshi, Peter Quoc Nguyen
  • Oligosaccharyltransferase polypeptide
    Patent number: 11365401
    Abstract: The disclosure relates to an oligosaccharyltransferase polypeptides and their use in the synthesis of glycoconjugates in bacterial cells; vaccines and immunogenic compositions comprising said glycoconjugates and their use in the prevention and/or treatment of bacterial infection. Bacterial expression system comprising said oligosaccharyltransferase polypeptides are also disclosed.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: June 21, 2022
    Assignee: London School of Hygiene and Tropical Medicine
    Inventors: Jon Cuccui, Brendan Wren
  • Complex BRET technique for measuring biological interactions
    Patent number: 11360096
    Abstract: Provided are methods for detecting protein interactions in a sample, the methods comprising: (a) detecting two or more polypeptides that when associated emit a first detectable signal in a first light emission spectrum; (b) contacting the two or more polypeptides with a third polypeptide conjugated to a dipole acceptor moiety that has a second light emission spectrum when excited within a light excitation spectrum, wherein the light excitation spectrum overlaps with the first light emission spectrum; and (c) detecting a second detectable signal emitted in the second light emission spectrum by the dipole acceptor moiety. Also provided are bioluminescent complexes comprising: (a) a first polypeptide conjugated to a dipole acceptor moiety, wherein the emits a first detectable signal in a first light emission spectrum.
    Type: Grant
    Filed: May 18, 2020
    Date of Patent: June 14, 2022
    Assignee: Duke University
    Inventors: Thomas Pack, Jeffrey Smith, Sudarshan Rajagopal, Marc Caron
  • Efficient product cleavage in template-free enzymatic synthesis of polynucleotides
    Patent number: 11359221
    Abstract: The present invention is directed to methods and kits for template-free enzymatic synthesis of polynucleotides that include or enable a step of efficiently cleaving the polynucleotide products from its initiator using endonuclease V activity and initiator with a 3?-penultimate deoxyinosine.
    Type: Grant
    Filed: February 11, 2020
    Date of Patent: June 14, 2022
    Assignee: DNA Script SAS
    Inventor: Sandrine Creton
  • C6/C5 co-fermented capable of relieving antagonism between high xylose utilization and high robustness and application thereof
    Patent number: 11359177
    Abstract: The present invention further discloses an application of Saccharomyces cerevisiae in the fermentation of a second generation fuel, ethanol, with a straw lignocellulose biomass hydrolysate as a raw material. Experiments prove that the strain of the prevent invention has an efficient co-fermentation capacity of C6/C5 while tolerating multiple inhibitors, and can consume all the glucose and xylose to produce ethanol; and the sugar-alcohol conversion rate is up to 0.43 g g?1. The strain and screening strategy used in the present invention provide technical reference and basis for the further breeding of a Saccharomyces cerevisiae strain which has a superior fermenting property and is produced by the second generation fuel, ethanol.
    Type: Grant
    Filed: August 13, 2021
    Date of Patent: June 14, 2022
    Assignee: QILU UNIVERSITY OF TECHNOLOGY
    Inventors: Xiaoming Bao, Jianzhi Zhao, Fangqing Wei, Hongxing Li, Zailu Li
  • Plastic degrading proteases
    Patent number: 11345906
    Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID N° 1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.
    Type: Grant
    Filed: September 30, 2020
    Date of Patent: May 31, 2022
    Assignee: CARBIOS
    Inventors: Alain Marty, Sophie Duquesne, Marie Guicherd, Marlene Vuillemin, Maher Ben Khaled
  • Acid-alpha glucosidase variants and uses thereof
    Patent number: 11339406
    Abstract: The present invention relates to variants of acid-alpha glucosidase and uses thereof.
    Type: Grant
    Filed: September 12, 2017
    Date of Patent: May 24, 2022
    Assignees: GENETHON, INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), UNIVERSITE D'EVRY VAL D'ESSONNE
    Inventors: Federico Mingozzi, Giuseppe Ronzitti
  • Engineered synthase for production of tryptophan derivatives and intransigent substrates
    Patent number: 11332729
    Abstract: This disclosure relates to modified tryptophan synthase and more particularly to modified beta-subunits of tryptophan synthase. The disclosure further relates to cells expressing such modified subunits and methods of producing non-canonical amino acids.
    Type: Grant
    Filed: August 24, 2017
    Date of Patent: May 17, 2022
    Assignee: California Institute of Technology
    Inventors: David K. Romney, Javier Murciano Calles, Jori E. Wehrmuller
  • Process for production of Galacto-oligosaccharides
    Patent number: 11306336
    Abstract: A process for production of galacto-oligosaccharides is provided. The process for production of galacto-oligosaccharides includes growing Sporobolomyces singularis in a fermentation medium, harvesting the said microbes from the fermentation medium, inoculating the said microbes with lactose solution in a reactor and incubating in an aerobic condition to convert lactose into galacto-oligosaccharides. The process also includes separating the galacto-oligosaccharides from the microbes; filtering thus obtained galacto-oligosaccharides; and optionally making the powder form of galacto-oligosaccharides. The process for producing galacto-oligosaccharides provides high yield and high purity.
    Type: Grant
    Filed: March 9, 2018
    Date of Patent: April 19, 2022
    Assignee: Tata Chemicals Limited
    Inventors: R Saravanan, S Narayanan, Deepak Jadhav, Manish Jain, Shajahan Shubethar, Shankar Lade, Manoj Gote, Ashok Kumar Dubey
  • DP04 polymerase variants
    Patent number: 11299725
    Abstract: Recombinant DPO4-type DNA polymerase variants with amino acid substitutions that confer modified properties upon the polymerase for improved single molecule sequencing applications are provided. Such properties may include enhanced binding and incorporation of bulky nucleotide analog substrates into daughter strands and the like. Also provided are compositions comprising such DPO4 variants and nucleotide analogs, as well as nucleic acids which encode the polymerases with the aforementioned phenotypes.
    Type: Grant
    Filed: May 4, 2020
    Date of Patent: April 12, 2022
    Assignee: Stratos Genomics, Inc.
    Inventors: Mark Stamatios Kokoris, Marc Prindle, Melud Nabavi, Craig Ostrander, Taylor Lehmann, Samantha Vellucci, Michael Kovarik, Jack Chase, Robert Busam, Miranda Lahman
  • T5 exonuclease-based method to identify DNA topoisomerase inhibitors
    Patent number: 11286514
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Grant
    Filed: October 16, 2020
    Date of Patent: March 29, 2022
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Fenfei Leng, Zifang Deng
  • Enzyme products
    Patent number: 11286474
    Abstract: The invention relates to a process for the manufacturing and purification of recombinant enzyme products, in particular of food enzyme products and the use thereof. The invention particularly relates to a process for the processing of enzyme products from a microbial fermentation broth by methods of separation, enzymatic treatment and filtration procedures.
    Type: Grant
    Filed: November 13, 2019
    Date of Patent: March 29, 2022
    Assignee: C-LECTA GMBH
    Inventors: Stefan Schoenert, Mathias Salomo, Thomas Schultchen, Andreas Vogel, Sabrina Koepke, Sebastian Bartsch, Birgit Brucher, Claudia Feller
  • Human alpha-galactosidase variants
    Patent number: 11278600
    Abstract: The present invention provides engineered human alpha-galactosidase polypeptides and compositions thereof. The engineered human alpha-galactosidase polypeptides have been optimized to provide improved stability under both acidic (pH<4.5) and basic (pH>7) conditions. The invention also relates to the use of the compositions comprising the engineered human alpha-galactosidase polypeptides for therapeutic purposes.
    Type: Grant
    Filed: August 5, 2020
    Date of Patent: March 22, 2022
    Assignee: Codexis, Inc.
    Inventors: Nicholas J. Agard, Mathew G. Miller, Xiyun Zhang, Gjalt W. Huisman
  • Augmented acid alpha-glucosidase for the treatment of Pompe disease
    Patent number: 11278601
    Abstract: A method for treating Pompe disease including administration of recombinant human acid ?-glucosidase having optimal glycosylation with mannose-6-phosphate residues in combination with an amount of miglustat effective to maximize tissue uptake of recombinant human acid ?-glucosidase while minimizing inhibition of the enzymatic activity of the recombinant human acid ?-glucosidase is provided.
    Type: Grant
    Filed: October 2, 2020
    Date of Patent: March 22, 2022
    Assignee: Amicus Therapeutics, Inc.
    Inventors: Hung V. Do, Richie Khanna, Russell Gotschall
  • Engineered meganucleases specific for recognition sequences in the Hepatitis B virus genome
    Patent number: 11274285
    Abstract: The present invention encompasses engineered meganucleases which recognize and cleave a recognition sequence within an open reading frame (ORF) of the genome of at least two genotypes of the Hepatitis B virus (HBV). The present invention also encompasses methods of using such engineered meganucleases in a pharmaceutical composition and in methods for treating or reducing the symptoms of a HBV infection, or treating hepatocellular carcinoma (HCC). Further, the invention encompasses pharmaceutical compositions comprising engineered meganuclease proteins, nucleic acids encoding engineered meganucleases, and the use of such compositions for treating HBV infections or HCC.
    Type: Grant
    Filed: October 28, 2020
    Date of Patent: March 15, 2022
    Assignee: Precision BioSciences, Inc.
    Inventors: Derek Jantz, James Jefferson Smith
  • T5 exonuclease-based method to identify DNA topoisomerase inhibitors
    Patent number: 11268129
    Abstract: The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.
    Type: Grant
    Filed: June 10, 2021
    Date of Patent: March 8, 2022
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Fenfei Leng, Zifang Deng