Patents Examined by Maryam A. Monshipouri
  • Patent number: 11261228
    Abstract: The present invention relates to insulin derivatives which, in comparison to insulin glulisine and similar derivatives, has a simplified process for synthesis. In particular, the present invention relates to insulin derivatives or physiologically tolerable salts thereof in which the motif Leucine-Proline-X-Threonine appears at the end of the B-chain, where X is any amino acid residue. Due to the nature of the process, the A-chain and B-chain must begin with a Glycine amino acid residue.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: March 1, 2022
    Inventor: Arri Russell Morris
  • Patent number: 11242507
    Abstract: The present invention relates to a novel mutant strain Aspergillus aculeatus E14-292 and a genetic modification process of said strain, wherein the mutant strain according to this invention can produce cellulase and xylanase more than the BCC199 (wild type). Moreover, the obtained enzymes can be used to digest the pretreatment bagasse to further produce sugars effectively.
    Type: Grant
    Filed: May 30, 2018
    Date of Patent: February 8, 2022
    Assignee: PTT Global Chemical Public Company Limited
    Inventors: Verawat Champreda, Surisa Suwannarangsee, Jantima Arnthong, Chatuphon Siamphan, Aphisit Poonsrisawat, Napong Ketsub, Mahattanee Phinyo, Wikanda Techanan, Natthawut Poomsila, Phatthanon Prasitchoke
  • Patent number: 11230723
    Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: January 25, 2022
    Assignee: Novozymes, Inc.
    Inventors: Jeffrey Shasky, Suchindra Maiyuran, Amanda Fischer
  • Patent number: 11224850
    Abstract: The invention provided methods and devices for performing sequential, regulated multiplex reactions in a single tube without the addition or removal of contents from the tube.
    Type: Grant
    Filed: February 20, 2020
    Date of Patent: January 18, 2022
    Assignee: GENETICS RESEARCH, LLC
    Inventors: Anthony P. Shuber, William Glover
  • Patent number: 11208442
    Abstract: The present invention provides a novel endo-?-N-acetylglucosaminidase that is isolated from a fungus belonging to the genus Rhizomucor and is active under high-temperature conditions; various mutant enzymes thereof; genes encoding the enzymes; a recombinant plasmid; a transformant transformed with the plasmid; and the like.
    Type: Grant
    Filed: December 1, 2017
    Date of Patent: December 28, 2021
    Assignee: Daiichi Sankyo Company, Limited
    Inventors: Hanako Ito, Yasunori Ono, Kensuke Nakamura
  • Patent number: 11203771
    Abstract: This disclosure relates to strategies for in vivo production of certain carbon-based products, for example, aminated aliphatic compounds having a carbon chain length of C5-C19.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: December 21, 2021
    Assignee: INV Nylon Chemicals Americas, LLC
    Inventors: Alexander Brett Foster, Jonathan Kennedy, Paul S. Pearlman
  • Patent number: 11198853
    Abstract: Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes synthesize insoluble alpha-glucan products having decreased molecular weight. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce insoluble alpha-glucan.
    Type: Grant
    Filed: March 7, 2019
    Date of Patent: December 14, 2021
    Assignee: NUTRITION & BIOSCIENCES USA 4, INC.
    Inventors: Yougen Li, Ellen D. Semke
  • Patent number: 11198877
    Abstract: Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester are disclosed. For example, genetically modified methanotrophs that are capable of generating 2,3-BDO; 1,4-BDO; isobutyraldehyde; isobutanol; 1-butanol; n-butanol; ethanol; fatty alcohols; and fatty acid methyl ester at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed. These microorganisms and methods make use of molecular switches to regulate gene expression.
    Type: Grant
    Filed: January 30, 2018
    Date of Patent: December 14, 2021
    Assignee: PRECIGEN, INC.
    Inventors: Mark Anton Held, Xinhua Zhao, Lily Yuin Chao, Na Trinh, James Kealey, Kevin Lee Dietzel
  • Patent number: 11180745
    Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: June 17, 2020
    Date of Patent: November 23, 2021
    Assignee: Novozymes A/S
    Inventors: Carsten Andersen, Signe E. Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoee-Nielsen, Randy Deinhammer, Xinyu Shen
  • Patent number: 11174501
    Abstract: A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.
    Type: Grant
    Filed: December 16, 2019
    Date of Patent: November 16, 2021
    Assignee: The University of Akron
    Inventor: Lu-Kwang Ju
  • Patent number: 11162116
    Abstract: The present disclosure relates to single and/or multi-stage, biological processes and systems for converting C5, C6, and/or disaccharide carbon sources to desirable products including monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The disclosure further relates to the processes carrying out fermentations of substrates in a single phase bioreactor or in a multiphase bioreactor comprising growth and production phases. Additionally, the processes and systems described herein result in high productivity and yield of the desired products due to intermittent or continuous removal of products to avoid inhibitory effects.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: November 2, 2021
    Assignee: BRASKEM S.A.
    Inventors: Jose Geraldo Da Cruz Pradella, Ane Fernanda Beraldi Zeidler, Ana Karina Brambilla Costa
  • Patent number: 11162088
    Abstract: Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.
    Type: Grant
    Filed: March 23, 2018
    Date of Patent: November 2, 2021
    Assignee: Hoffmann-La Roche Inc.
    Inventors: Martin Schatte, Mara Boenitz-Dulat, Frank Bergmann
  • Patent number: 11155798
    Abstract: The invention relates to enzyme compositions comprising a glucoamylase, an alpha-amylase, and optionally a cellulolytic composition and/or a protease. The invention also relates to the use thereof in processes of producing sugars and/or fermentation products from starch-containing material by saccharifying and/or fermenting starch-containing material at a temperature below the initial gelatinization temperature.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: October 26, 2021
    Assignee: NOVOZYMES A/S
    Inventors: Joseph Jump, Nathaniel Kreel, Jeremy Saunders, Michael John Akerman
  • Patent number: 11149291
    Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: October 19, 2021
    Assignee: BioMediCan, Inc.
    Inventors: Maxim Mikheev, Difeng Gao
  • Patent number: 11142751
    Abstract: A system for modifying the genome of Clostridium strains is provided based on a modified endogenous CRISPR array. The application also describes Clostridium strains modified for enhanced butanol production wherein the modified strains are produced using the novel CRISPR-Cas system.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: October 12, 2021
    Assignee: AUBURN UNIVERSITY
    Inventors: Yi Wang, Jie Zhang
  • Patent number: 11136373
    Abstract: The present invention provides a fermentation process for improving the production level of a recombinant human collagen. The process comprises inoculating a sterilized fermentation medium with a Pichia yeast solution, then performing fermentation culturing for 14-18 hrs, adding methanol to induce the expression, upon which sodium pyruvate is added to the fermentation medium, wherein the amount of the sodium pyruvate added is 0.01-10 g/L. In the fermentation process of the present invention, the sodium pyruvate is added in the methanol-induced expression stage, so that the biosynthesis rate of the recombinant human collagen is improved; and a continuous feed-batch mode is adopted, so that the biosynthesis rate of the recombinant human collagen is further improved, and the fermentation time is shortened. Meanwhile, the expression level of the recombinant human collagen is increased, the fermentation level is increased by more than 20%, and the production cost is reduced.
    Type: Grant
    Filed: October 18, 2016
    Date of Patent: October 5, 2021
    Assignee: JIANGSU JLAND BIOTECH CO., LTD.
    Inventors: Shulin Yang, Erfeng Du, Jianmin Huang, Lihu Gao, Jianfeng Zhao, Hai Tao, Liping Feng, Aimei Zhou
  • Patent number: 11124806
    Abstract: Described are methods for the production of isobutene comprising the enzymatic conversion of 3-methylcrotonic acid into isobutene wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-methylcrotonyl-CoA into 3-methylcrotonic acid or wherein said 3-methylcrotonic acid is obtained by the enzymatic conversion of 3-hydroxyisovalerate (HIV) into 3-methylcrotonic acid. It is described that the enzymatic conversion of 3-methylcrotonic acid into isobutene can, e.g., be achieved by making use of a 3-methylcrotonic acid decarboxylase, preferably an FMN-dependent decarboxylase associated with an FMN prenyl transferase, an aconitate decarboxylase (EC 4.1.1.6), a methylcrotonyl-CoA carboxylase (EC 6.4.1.4), or a geranoyl-CoA carboxylase (EC 6.4.1.5).
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: September 21, 2021
    Assignees: Global Bioenergies, Scientist of Fortune, SA
    Inventors: Mathieu Allard, Maria Anissimova, Philippe Marlière
  • Patent number: 11118194
    Abstract: The present disclosure provides modified site-directed modifying polypeptides, and ribonucleoproteins comprising the modified polypeptides. The modified site-directed modifying polypeptides are modified for passive entry into target cells. The modified site-directed modifying polypeptides are useful in a variety of methods for target nucleic acid modification, which methods are also provided.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: September 14, 2021
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, Brett T. Staahl, Anirvan Ghosh
  • Patent number: 11104907
    Abstract: The invention relates to engineering of acetyl-CoA metabolism in yeast and in particular to production of acetyl-CoA in a non-ethanol producing yeast lacking endogenous gene(s) encoding pyruvate decarboxylase and comprising a heterologous pathway for synthesis of cytosolic acetyl-CoA.
    Type: Grant
    Filed: June 12, 2020
    Date of Patent: August 31, 2021
    Assignee: BIOPETROLIA AB
    Inventors: Jens Nielsen, Verena Siewers, Anastasia Krivoruchko, Yiming Zhang, Zongjie Dai
  • Patent number: 11098336
    Abstract: Recombinant enzymes BesA, BesB, BesC, BesD and/or BesE are used generate non-canonical amino acids comprising a useful functional group, such as an alkynlyl, alkenyl or halogen.
    Type: Grant
    Filed: October 13, 2019
    Date of Patent: August 24, 2021
    Assignee: The Regents of the University of California
    Inventors: Michelle C. Y. Chang, Jorge A. Marchand Benmaman, Monica E. Neugebauer