Abstract: The present disclosure provides a novel aldehyde synthase gene having activity of synthesizing a medium-chain aldehyde. Such gene encodes a protein comprising the amino acid sequence as shown in SEQ ID NO: 2.
Type:
Grant
Filed:
March 3, 2020
Date of Patent:
January 19, 2021
Assignees:
Toyota Jidosha Kabushiki Kaisha, Kyoto University
Abstract: The present disclosure pertains to methods of growing bacterial host cells in a low-density polyethylene (LDPE) bag to produce plasmid DNA or express recombinant protein. The LDPE bag is filled with media, an antibiotic, and host bacterial cells that have been transformed with plasmid DNA encoding a protein of interest. The LDPE bag is sealable to the external environment and incubated at a growth temperature until a desired concentration of bacteria is achieved. When plasmid DNA is desired, host cells are harvested and plasmid DNA is separated from host cell components. When recombinant protein is desired, expression is induced while host cells are in the LDPE bag, followed by the harvest and separation of the recombinant protein. The LDPE bags are sterile and conducive to bacterial growth equal to or greater than that afforded by conventional shake flasks under similar growth conditions.
Abstract: The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. Such a variant polypeptide may be used in the preparation of a baked product.
Type:
Grant
Filed:
December 19, 2017
Date of Patent:
January 12, 2021
Assignee:
DSM IP ASSETS B.V.
Inventors:
Rene Marcel De Jong, Chantal Christis, Neil Carr
Abstract: The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material.
Abstract: A method is described for producing an objective substance, for example, an aldehyde such as vanillin. The objective substance is produced from a carbon source or a precursor of the objective substance by using a microorganism having an ability to produce the objective substance, wherein the microorganism has been modified to have a specific carboxylic acid reductase (CAR) gene, such as a Gordonia CAR gene, Novosphingobium CAR gene, or Coccomyxa CAR gene.
Abstract: Provided are compositions and methods for compound discovery. Modified yeast that have their endogenous yeast shikimate pathway disrupted or deleted, and replaced with homologous pathway genes from one or more distinct organisms, are provided and used in assays of test agents. The homologous pathway genes are designed to supplement the disrupted or deleted shikimate pathway genes. The assays are designed to identify whether or not the test agents can interfere with the function of enzymes in the shikimate pathway from organisms that are distinct from the yeast avatar hosts. In embodiments, the disruption/deletion of the yeast endogenous shikimate pathway results in the yeast being incapable of producing chorismic acid.
Type:
Grant
Filed:
April 4, 2019
Date of Patent:
December 15, 2020
Assignees:
New York University, Neochromosome Inc., The Johns Hopkins University
Inventors:
Jef Boeke, Leslie Mitchell, Joe De Angelo, Joel S. Bader
Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.
Type:
Grant
Filed:
July 13, 2020
Date of Patent:
December 15, 2020
Assignee:
Codexis, Inc.
Inventors:
Oscar Alvizo, David Elgart, Robert Kevin Orr, James Nicholas Riggins, Anna Fryszkowska, Katrina W. Lexa, Xiang Yi, Da Duan, Courtney Dianne Moffett, Nikki Dellas, Vesna Mitchell
Abstract: The present invention relates to glucoamylase variants having improved thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
September 19, 2018
Date of Patent:
December 15, 2020
Assignee:
NOVOZYMES A/S
Inventors:
Esben Peter Friis, Leonardo De Maria, Jesper Vind, Thomas A. Poulsen, Allan Svendsen, Steffen Danielsen, Rolf T. Lenhard, Henrik Friis-Madsen, Lars K. Skov
Abstract: A method for treating Pompe disease including administration of recombinant human acid ?-glucosidase having optimal glycosylation with mannose-6-phosphate residues in combination with an amount of miglustat effective to maximize tissue uptake of recombinant human acid ?-glucosidase while minimizing inhibition of the enzymatic activity of the recombinant human acid ?-glucosidase is provided.
Type:
Grant
Filed:
April 11, 2018
Date of Patent:
December 8, 2020
Assignee:
Amicus Therapeutics, Inc.
Inventors:
Hung V. Do, Richie Khanna, Russell Gotschall
Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.
Type:
Grant
Filed:
June 19, 2017
Date of Patent:
December 1, 2020
Assignee:
DKK-TOA Corporation
Inventors:
Kenichi Noda, Satoshi Yawata, Ai Shimomura
Abstract: Reagents and methods for the digital analysis of proteins or peptides are provided. Specifically provided herein are proteins for identifying the N-terminal amino acid or N-terminal phosphorylated amino acid of a polypeptide. Also, an enzyme for use in the cleavage step of the Edman degradation reaction and a method for using this enzyme are described.
Abstract: The present invention encompasses engineered meganucleases which recognize and cleave a recognition sequence within an open reading frame (ORF) of the genome of at least two genotypes of the Hepatitis B virus (HBV). The present invention also encompasses methods of using such engineered meganucleases in a pharmaceutical composition and in methods for treating or reducing the symptoms of a HBV infection, or treating hepatocellular carcinoma (HCC). Further, the invention encompasses pharmaceutical compositions comprising engineered meganuclease proteins, nucleic acids encoding engineered meganucleases, and the use of such compositions for treating HBV infections or HCC.
Abstract: Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.
Type:
Grant
Filed:
March 7, 2017
Date of Patent:
November 24, 2020
Assignee:
Genomatica, Inc.
Inventors:
Jay D. Keasling, Zhihao Hu, Chris Somerville, George Church, David Berry, Lisa C. Friedman, Andreas Schirmer, Shane Brubaker, Stephen B. Del Cardayre
Abstract: Mutant thioesterases having enhanced medium chain substrate activity, polynucleotides encoding and configured to express the mutant thioesterases in a transformed host cell, host cells transformed to contain the polynucleotides, and methods of using same.
Type:
Grant
Filed:
March 19, 2019
Date of Patent:
November 24, 2020
Assignee:
WISCONSIN ALUMNI RESEARCH FOUNDATION
Inventors:
Brian F. Pfleger, Nestor Hernandez-Lozada, Rung-Yi Lai
Abstract: Disclosed are a recombinant microorganism capable of producing methyl anthranilate, wherein the recombinant microorganism is obtained by introducing an aamt1 gene into a microorganism having the capacity to produce anthranilic acid (ANT), and a method of producing methyl anthranilate using the same. The recombinant microorganism is capable of producing methyl anthranilate using only a purely biological process from renewable carbon-circulating biomass without chemical catalytic reaction, thus having an advantage of enabling mass production of high value-added methyl anthranilate in a very economical manner based on an environmentally friendly and simple production process.
Type:
Grant
Filed:
February 15, 2020
Date of Patent:
November 17, 2020
Assignee:
KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
Abstract: The present invention relates to novel proteases, more particularly to protease variants having improved activity compared to the protease of SEQ ID NO:1 and the uses thereof for degrading polyester containing material, such as plastic products. The proteases of the invention are particularly suited to degrade polylactic acid, and material containing polylactic acid.
Type:
Grant
Filed:
December 15, 2017
Date of Patent:
November 10, 2020
Assignee:
CARBIOS
Inventors:
Alain Marty, Sophie Duquesne, Marie Guicherd, Marlene Vuillemin, Maher Ben Khaled
Abstract: The invention relates to an enzymatic method for producing 2-hydroxy-4-methylmercaptobutanoic acid from 3-methylthio-propanal (3-methylmercaptopropanal (MMP) or “methional”) and carbon dioxide.
Type:
Grant
Filed:
April 8, 2018
Date of Patent:
October 27, 2020
Assignee:
Evonik Operations GmbH
Inventors:
Arne Skerra, Julia Martin, Harald Jakob, Daniel Fischer
Abstract: Disclosed herein is a compositions-of-matter comprising a cross-linked polymer, the cross-linked polymer comprising a plurality of resilin polypeptide moieties, and at least one polymeric moiety covalently cross-linked to a plurality of the resilin polypeptide moieties via at least one cross-linking moiety, the cross-linking moiety being devoid of a biphenyl moiety. Further disclosed herein is a process for preparing the composition-of-matter, a composite material comprising the composition-of-matter and at least one additional polymeric substance bound to the cross-linked polymer, an article-of-manufacturing comprising the composition-of-matter and/or composite material, and a method of treating tissue damage or loss by implanting said article-of-manufacturing in a subject.
Type:
Grant
Filed:
November 5, 2014
Date of Patent:
October 13, 2020
Assignees:
CollPlant Ltd., Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
Inventors:
Oded Shoseyov, Sigal Meirovitch, Shaul Lapidot, Amit Rivkin
Abstract: A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing in a medium a bacterium having an L-amino acid-producing ability, which has been modified so that the activity of a c1795 protein is reduced or the activity of a protein of which the expression is repressed by a c1795 protein is increased, and collecting the L-amino acid from the medium or cells of the bacterium.
Type:
Grant
Filed:
January 28, 2020
Date of Patent:
September 29, 2020
Assignee:
AJINOMOTO CO., INC.
Inventors:
Hiromi Noguchi, Yo Nishiyama, Yoshihiro Ito