Abstract: Methods and compositions related to the engineering of a protein with L-cyst(e)ine degrading enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-?-lyase comprising one or more amino acid substitutions and capable of degrading L-cyst(e)ine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with L-cyst(e)ine using the disclosed proteins or nucleic acids.
Type:
Grant
Filed:
August 29, 2014
Date of Patent:
July 30, 2019
Assignee:
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
Abstract: The invention relates to processes of producing a fermentation product, comprising liquefying a starch containing material with an alpha-amylase; pre-saccharifying and/or saccharifying and fermenting using a fermentation organism in the presence of a carbohydrate source generating enzyme and a cellulolytic composition The invention also relates to methods of dewatering whole stillage.
Type:
Grant
Filed:
November 27, 2017
Date of Patent:
July 30, 2019
Assignee:
NOVOZYMES A/S
Inventors:
Thomas Rasmussen, Jeremy Saunders, James Croonenberghs, Zhengfang Kang, Joyce Craig, Michael John Akerman
Abstract: The present invention includes compositions and methods for treating diseases or disorders associated with pathological calcification or pathological ossification. In certain embodiments, the diseases or disorders are selected from the group consisting of Generalized Arterial Calcification of Infancy (GACI), Idiopathic Infantile Arterial Calcification (IIAC), Ossification of the Posterior Longitudinal Ligament (OPLL), hypophosphatemic rickets, osteoarthritis, calcification of atherosclerotic plaques, PXE, hereditary and non-hereditary forms of osteoarthritis, ankylosing spondylitis, hardening of the arteries occurring with aging, calciphylaxis resulting from end stage renal disease and progeria.
Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Grant
Filed:
March 7, 2016
Date of Patent:
July 16, 2019
Assignee:
Novozymes A/S
Inventors:
Carsten Andersen, Signe Larsen, Esben Peter Friis, Pernille Ollendorff Micheelsen, Anders Viksoee-Nielsen, Randy Deinhammer, Xinyu Shen
Abstract: A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product.
Type:
Grant
Filed:
August 14, 2017
Date of Patent:
July 16, 2019
Assignee:
Battelle Energy Alliance, LLC
Inventors:
David N. Thompson, William A. Apel, Vicki S. Thompson, Thomas E. Ward
Abstract: Compounds and methods are providing involving RebH variants with improved properties. directed evolution based on random mutagenesis was employed to generate a series of RebH variants. RebH variants with improved thermostability and increased activity at elevated temperatures were generated.
Type:
Grant
Filed:
November 26, 2014
Date of Patent:
July 9, 2019
Assignee:
The University of Chicago
Inventors:
Jared C. Lewis, Catherine Poor, Mary Andorfer, James Payne
Abstract: The present disclosure provides activated formylglycine-generating enzymes (FGE), methods of producing activated FGE, and their use in methods of producing a protein comprising a formylglycine (FGly) residue. The methods of producing activated FGE, as well as methods of use of activated FGE in producing FGly-containing proteins, include both cell-based and cell-free methods. Compositions and kits that find use, e.g., in practicing the methods of the present disclosure are also provided.
Type:
Grant
Filed:
January 4, 2018
Date of Patent:
July 9, 2019
Inventors:
David Rabuka, Gregory W. deHart, Patrick Holder, Jeanne Baker
Abstract: The invention is directed toward mutant, non-wild-type organophosphorus acid anhydrolase enzymes having three site mutations, methods of production, and methods of use to effectively degrade toxic organophosphorus compounds, most preferably GP (2,2?-dimethylcyclopentyl methylphosphonofluoridate).
Type:
Grant
Filed:
January 26, 2018
Date of Patent:
July 2, 2019
Assignee:
The United States of America as Represented by the Secretary of the Army
Inventors:
Steven P Harvey, Melissa M Dixon, Mark A Guelta, Leslie R McMahon
Abstract: The present invention relates to variants of a parent glucoamylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the glucoamylase variants.
Type:
Grant
Filed:
December 14, 2017
Date of Patent:
June 11, 2019
Assignee:
NOVOZYMES A/ S
Inventors:
Jesper Vind, Esben Peter Friis, Thomas Agsersten Poulsen, Michael Skjøt, Peter Kamp Hansen, Frank Winther Rasmussen, Steen Krogsgaard, Joyce Craig, Guillermo Coward-Kelly, Keiichi Ayabe
Abstract: Described herein are variant pol6 polymerase having at least having at least two substitutions, wherein at least one of the substitutions is at position S366 of SEQ ID NO: 2. The disclosed polymerases may be coupled to alpha-hemolysin nanopores, and may be used in nanopore-based sequencing apparatuses.
Type:
Grant
Filed:
February 1, 2016
Date of Patent:
June 4, 2019
Assignees:
Roche Molecular Systems, Inc., Genia Technologies, Inc.
Inventors:
Aruna Ayer, Arkadiusz Bibillo, Cleoma Renetta Arnold, Charles Wayan Schwab, Eileen Thai, Ilya Lederman, Colin Alexander McGaw, Tyler O'Brien Shultz, Barbara Eckert, Shawn Suko, Mara Boenitz-Dulat, Bigna Woersdoerfer, David Daniel Wunderlich
Abstract: Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
Type:
Grant
Filed:
October 18, 2018
Date of Patent:
June 4, 2019
Assignee:
E I DU PONT DE NEMOURS AND COMPANY
Inventors:
Mark S. Payne, Yefim Brun, Hongxian He, Thomas Scholz
Abstract: A process for refolding recombinant chymotrypsin produced from prokaryote host cells is described. In particular, the present invention provides a process for refolding recombinant chymotrypsin produced from E. coli is described.
Type:
Grant
Filed:
November 13, 2015
Date of Patent:
May 28, 2019
Assignee:
MERCK SHARP & DOHME CORP.
Inventors:
Mark C. Sleevi, Jack Lile, Bryn Grimison
Abstract: Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-?-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-?-?-lipoic acid.
Type:
Grant
Filed:
March 4, 2015
Date of Patent:
May 21, 2019
Assignee:
East China University of Science and Technology
Inventors:
Jianhe Xu, Yujun Zhang, Gaowei Zheng, Jiang Pan
Abstract: The present invention relates to the field of genetic engineering and enzyme engineering, and more particularly relates to a maltooligosyl trehalose synthase mutant and its application. The present invention provides a series of maltooligosyl trehalose synthase mutants with improved enzyme activity.
Abstract: The present invention relates to a multi-functional peptide benefiting expression, purification, stabilization and catalytic efficiency of recombinant proteins, which relates to the field of enzyme engineering and protein purification. The present invention provides a self-assembling amphipathic peptide, which fused with proteins including alkaline polygalacturonate lyase (PGL), lipoxygenase (LOX) and green fluorescent protein (GFP), which leads to successful purification by the nickel affinity chromatography with recovery rates were 47.01%, 39.01% and 56.1%, respectively. Furthermore, the expression quantity and thermostability of the three proteins were enhanced in different degree. Of which the half-life of the PGL-S1v1 and LOX-S1v1 were 2.3 and 3.8-fold as compared with the corresponding wild-type and the specific activity were 1.1 and 1.9-fold increase, respectively. The crude enzyme activity of PGL-S1v1 was 9-fold increase than the PGL.
Type:
Grant
Filed:
July 24, 2017
Date of Patent:
April 30, 2019
Assignee:
JIANGNAN UNIVERSITY
Inventors:
Song Liu, Weixin Zhao, Jian Chen, Guocheng Du
Abstract: The present disclosure is generally directed to glycosyl hydrolase enzymes, compositions comprising such enzymes, and methods of using the enzymes and compositions, for example for the saccharification of cellulosic and hemicellulosic materials into sugars.
Type:
Grant
Filed:
July 27, 2015
Date of Patent:
April 30, 2019
Assignee:
DANISCO US INC.
Inventors:
Benjamin Bower, Megan Yee Hsi, Thijs Kaper, Bradley R. Kelemen, Suzanne E. Lantz, Edmund A. Larenas, Colin Mitchinson, Steven Kim, William D. Hitz, Mark Emptage, Keith Dumont Wing
Abstract: The present invention relates to novel polypeptides comprising an ice-binding capability resulting in an ice crystal formation and/or growth reducing or inhibiting activity. The present invention also relates to an edible product and to a solid support comprising the novel polypeptide. Furthermore, the present invention also relates to a method for producing the novel polypeptide and to different uses of the novel polypeptide.
Type:
Grant
Filed:
January 21, 2016
Date of Patent:
April 23, 2019
Assignee:
ROSKILDE UNIVERSITET
Inventors:
Hans Ramløv, Casper Wilkens, Anders Løbner-Olesen
Abstract: The present invention relates to a microorganism of the genus Corynebacterium, which has an enhanced activity of aspartate ammonia-lyase and/or aspartate aminotransferase, and thus has an enhanced ability to produce L-arginine, and to a method of producing L-arginine using the microorganism of the genus Corynebacterium.
Type:
Grant
Filed:
April 23, 2014
Date of Patent:
April 23, 2019
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Hyun Ae Bae, Min Gyeong Kang, Han Hyoung Lee, Hye Won Kim, Sung Gun Lee
Abstract: The present application provides a culture medium for producing ?-glucan. The culture medium comprises: a carbon source, lecithin and an ascorbic acid. The present application further provides a method for producing ?-glucan, the method comprising culturing Aureobasidium pullulans in the culture medium for fermentation. The Aureobasidium pullulans is advantageous in producing no melanin; as a result, the yield of ?-glucan can be increased and the waste can be reduced with the improved culture medium composition and culturing method. A biological sample of the Aureobasidium pullulans described in the present specification is deposited at National Institute of Technology and Evaluation (NITE), NITE Patent Microorganisms Depositary (NPMD) on Oct. 19, 2016 under the access number NITE BP-02372.
Type:
Grant
Filed:
March 2, 2018
Date of Patent:
April 16, 2019
Assignee:
Food Industry Research and Development Institute