Patents Examined by Michael Hobbs
  • Patent number: 9939357
    Abstract: A cartridge for holding and dispensing streaking applicators for an automated streaking apparatus having a cartridge holder, the cartridge including: a case with a dispensing end, a longitudinal axis and an elongate slot generally parallel to the longitudinal axis, the dispensing end including an applicator dispensing position where one applicator can be at least partially accessible from outside the case; and an applicator support member constrained within the case for longitudinal movement therein and being able to carry a stack of applicators; the elongate slot being capable of operatively receiving a biasing member when the cartridge is in the cartridge holder to urge the support member towards the dispensing end and an applicator into the dispensing position.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: April 10, 2018
    Inventors: Rajiv Gupta, Stephen Lewis Leckenby, Phillip James Duncan, Ryan Andrew Annear, Colin William Potter
  • Patent number: 9925540
    Abstract: There is provided a nucleic acid analysis apparatus including a heating unit configured to apply heat by contacting a microchip, and a chip holding unit configured to change a position between a first holding position that holds the microchip in midair and a second holding position that holds the microchip in contact with the heating unit.
    Type: Grant
    Filed: May 27, 2013
    Date of Patent: March 27, 2018
    Inventors: Toshio Watanabe, Masayoshi Akita, Takanori Anaguchi
  • Patent number: 9920292
    Abstract: System and methods are provided for initiating a cell culture. The systems and methods include a first enclosure containing a thermal mass positioned on a surface area of a platform. The systems and methods include a second enclosure, containing a culture fluid and a gas mixture, placed on the first enclosure such that the first enclosure is positioned between the second enclosure and the platform. The systems and methods further include a cover secured on the second enclosure, and generating first thermal energy on the surface area of the platform and second thermal energy from the cover.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: March 20, 2018
    Inventors: Chengkun Zhang, Reginald Donovan Smith, Kenneth Roger Conway, Anshika Bajaj
  • Patent number: 9909171
    Abstract: The present invention provides miniaturized instruments for conducting chemical reactions where control of the reaction temperature is desired or required. Specifically, this invention provides chips and optical systems for performing and monitoring temperature-dependent chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost amplification of nucleic acids.
    Type: Grant
    Filed: September 17, 2015
    Date of Patent: March 6, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: Victor Joseph, Amjad Huda, Alnoor Shivji, Jie Zhou
  • Patent number: 9902928
    Abstract: The present invention provides a production module for large-scale production of cells and/or cell-derived products such as antibodies or virus; a production suite comprising a plurality of functionally connected production modules of the invention; and a method for large-scale production of cells and/or cell-derived products using the production modules and/or production suites of the invention.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: February 27, 2018
    Inventors: Mark Hirschel, Robert J. Wojciechowski, Kim Arneson
  • Patent number: 9901927
    Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: February 27, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Benjamin J. Hindson, Kevin D. Ness, Billy W. Colston, Jr., Fred P. Milanovich, Donald A. Masquelier
  • Patent number: 9844779
    Abstract: A microfluidic device is provided. The microfluidic device includes a first transparent, solid support layer. A first polymeric layer defining at least one chamber is attached to the first transparent, solid support layer. A semi-permeable membrane is attached to the first polymeric layer. A second polymeric layer is attached to the opposite side of the semi-permeable membrane from the first polymeric layer. The second polymeric layer has a thickness of less than 300 microns and defines at least one chamber positioned to overlap with at least one chamber in the first polymeric layer. A first manifold structure is attached to an input end of at least one chamber and a second manifold structure is attached to an output end of at least one chamber.
    Type: Grant
    Filed: January 13, 2012
    Date of Patent: December 19, 2017
    Inventors: Jeffrey T. Borenstein, Joseph L. Charest, Joseph Cuiffi, Alla Epshteyn, Angela B. Holton, James Ching-Ming Hsiao
  • Patent number: 9829477
    Abstract: A planar patch clamp device is disclosed, which can be used for culturing a neuron in the device so as to form a neuron network, and detecting an electrical property of the neuron that forms the neuron network. The planar patch clamp device includes a plurality of protrusions formed on a first surface, an extracellular matrix forming substance which is coated on the peripheries of a through hole, and electrode sections.
    Type: Grant
    Filed: March 21, 2013
    Date of Patent: November 28, 2017
    Assignee: Japan Science and Technology Agency
    Inventors: Tsuneo Urisu, Zhi-hong Wang, Hidetaka Uno, Miho Saitoh, Yasutaka Nagaoka
  • Patent number: 9822043
    Abstract: A system for the continuous production of a liquid fertilizer is provided. The system operates by feeding a compost material at a continuous rate to an extractor. The extractor uses a belt system to convey the compost along a path. Sprayers above the belt spray water or similar liquid water based substance onto the compost. The water absorbs nutrients and other components of the compost and is then drained through the belt to a receptacle to store the liquid fertilizer.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: November 21, 2017
    Inventor: Kevin Gunn
  • Patent number: 9816067
    Abstract: An automated biological growth and dispensing apparatus utilizing a modular growing tank which is removable for replacement by another growing tank. Mechanisms for the delivery of air, water and/or nutrients are adapted to permit the growing tank to be readily coupled and uncoupled for easy and inexpensive replacement. Mechanisms for agitation may be integral and removable with the growing tank or may be adapted for a quick connection and disconnection with the growing tank. The growing tank may be disposable and each new growing tank may be provided as a sealed container including a starting amount of a biomass and/or nutrient.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: November 14, 2017
    Assignee: OP-Hygiene IP GmbH
    Inventor: Heiner Ophardt
  • Patent number: 9790485
    Abstract: Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by positioning a suitable element so as to reduce and/or eliminate a headspace size at a sample/gas interface. An interaction between the acoustic energy and the sample may be controlled, e.g., by using a headspace control element positioned at least partially in the sample that helps to reduce splashing or other sample ejection that would otherwise occur.
    Type: Grant
    Filed: February 27, 2015
    Date of Patent: October 17, 2017
    Assignee: Covaris, Inc.
    Inventor: James A. Laugharn, Jr.
  • Patent number: 9781918
    Abstract: A substrate unit for cryopreservation of a biological sample includes: a substrate platform having on a front side thereof a cultivation surface for receiving the biological sample; a first chamber including the cultivation surface of the substrate platform, wherein the first chamber is configured for receiving a cultivation liquid; a second chamber configured for receiving a temperature control medium; and a chamber bracket for receiving the substrate unit in a cryopreservation device in a pivotable manner, wherein the first and second chambers are connected to each other in an adjacent manner, and the substrate platform forms a separating wall between the first chamber and the second chamber, and wherein the back side of the substrate platform faces the second chamber. The invention further relates to a cryopreservation device, wherein the substrate unit is arranged in a rotatable manner, and a method for the cryopreservation of a biological sample.
    Type: Grant
    Filed: July 1, 2013
    Date of Patent: October 10, 2017
    Assignee: Fraunhofer-Gesellschaft zur Foerderung der angewandten Forschung e.V.
    Inventors: Heiko Zimmermann, Axel Beier, Julia Neubauer, Guenter R. Fuhr
  • Patent number: 9776146
    Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: October 3, 2017
    Assignee: Life Technologies Corporation
    Inventors: Jonathan Schultz, John Nobile, Brian Reed, Prasanna Krishnan Thwar, Todd Roswech
  • Patent number: 9778277
    Abstract: An apparatus (2) for the automatic testing of substances for carcinogenicity comprises: a plate support (3) on which at least one micro-well plate (1) can be placed; a movable pipetting unit (4) comprising a predetermined number of pipettes (40); a number of containers (5) containing a liquid culture medium (50), the number of containers (5) corresponding to the number of pipettes (40) of the pipetting unit (4); a dish support (6) on which a corresponding number of dishes (60) can be placed, each having a bottom and an upstanding side wall (601); a plurality of spinners (63) for spinning the dishes (60); a plurality of suction cups (62) for placing a lid (600) on each of the dishes; and a plurality of laterally arranged belts (64,65) for transporting the closed dishes (60) to an intermediate storage (8).
    Type: Grant
    Filed: March 19, 2010
    Date of Patent: October 3, 2017
    Assignee: F. Hoffmann-La Roche AG
    Inventors: Dieter Voegelin, Patrick Iaiza, Thomas Zumstein, Tom Kissling
  • Patent number: 9778153
    Abstract: A microfluid device for producing diffusively built gradients comprising a bottom plate and a cover plate, wherein the cover plate has recesses and is connected to the bottom plate in a liquid-tight manner so that the recesses form at least two reservoirs and one observation chamber, which connects the reservoir, a reservoir can be filled particularly through an inlet/outlet through the cover plate, and the cross-sectional surface of the observation chamber is at least 5 times, preferably at least 200 times smaller at the aperture of the observation chamber into one of the reservoirs than the maximum cross-sectional surface of the reservoir in parallel to this cross-sectional surface of the observation chamber.
    Type: Grant
    Filed: December 10, 2013
    Date of Patent: October 3, 2017
    Assignee: IBIDI GMBH
    Inventor: Roman Zantl
  • Patent number: 9765292
    Abstract: A bioreactor for preferably three-dimensional cell culturing comprises a scaffold chamber, a first tube, a second tube and a first valve with a scaffold adapter, a tube adapter and a medium adapter. The first valve has a housing with a longitudinal female portion ending in an opening and a longitudinal actuator being arranged through the opening of the female portion of the housing such that the actuator is arranged partially inside the housing and partially outside the housing, wherein the actuator of the first valve is axially moveable relative to the housing of the first valve between a first position in which the first valve is in the operation position and a second position in which the first valve is in the medium change position. By providing the actuators in the first valve which is applied by axial movements, operation of the bioreactor can be comparably simple and safe.
    Type: Grant
    Filed: June 4, 2013
    Date of Patent: September 19, 2017
    Assignees: University Hospital of Basel, Cellec Biotek AG
    Inventors: Elia Piccinini, David Wendt, Adam Papadimitropoulos, Laura Spinelli, Stefania Adele Riboldi, Francesco Giovanni Greco, Peter Iwatschenko
  • Patent number: 9758754
    Abstract: A cell separation and culture device having a porous substrate; and a patterned carbon powder layer having a plurality of hollow regions, formed on an upper surface of the porous substrate by a forming manner; wherein the thickness of the patterned carbon powder layer is 0.04-0.08 mm. The cell separation and culture device is able to separate, detect or culture cells with various size and shape. The cell separation and culture device of present invention also simplifies the process of cell separation, detection and culture; therefore, it is accomplished within a very short time.
    Type: Grant
    Filed: January 29, 2015
    Date of Patent: September 12, 2017
    Assignee: National Tsing Hua University
    Inventors: Da-Jeng Yao, Chih-Chung Chen
  • Patent number: 9757707
    Abstract: The present invention provides methods, systems, assemblies, and articles for capturing single cells with a capture chip. In certain embodiments, the capture chip comprises a substrate comprising a plurality of cell-sized dimples or wells that each allow a single cell to be captured from a cell suspension. In some embodiments, the dimples or wells of the capture chip align with the holes or wells of a multi-well through-hole chip, and/or a multi-well chip, such that the cell, or the contents of the single cell, may be transferred to a corresponding well of the multi-well chip. In particular embodiments, the bottom of each dimple or well of the capture chip has a positive electrical charge sufficient to attract cells from a cell suspension flowing over the dimples or wells.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: September 12, 2017
    Assignee: Takara Bio USA, Inc.
    Inventors: Syed A. Husain, Bradley L. Griswold, Michael Slater, Patricio A. Espinoza Vallejos, Jude Dunne, Glenn Hein, Maithreyan Srinivasan
  • Patent number: 9745228
    Abstract: A composting facility includes: a floor for receiving compostable material thereon, wherein the floor is constructed above-grade; a dovetail extending from the floor to allow ingress of a vehicle from a grade level to the floor; one or more channels defined within the floor and extending through the dovetail; a covering extending over the one or more channels to maintain the compostable material away from the channel, the covering defining at least one aperture extending from above the covering into the one or more channels for allowing flowthrough of gases; and an air source for providing air through the channels to provide cooling characteristics to the compostable material or oxygen to aid an aerobic process associated with composting. In operation, liquids draining from the composting material pass through the at least one aperture into the channel and out through a portion of the channel extending through the dovetail.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: August 29, 2017
    Assignee: McGill Environmental Systems of N.C., Inc.
    Inventor: Michael Noel Lyons
  • Patent number: 9738862
    Abstract: A bioreactor apparatus includes a vessel establishing an interior space environmentally separable from an exterior space outside of the vessel, an agitation system including mixing means arranged in the interior space and drive means adapted to rotate the mixing means. The drive means includes a drive motor that is arranged in the interior space.
    Type: Grant
    Filed: November 6, 2013
    Date of Patent: August 22, 2017
    Inventors: Yrjo Virtanen, Vesa Joutsjoki