Abstract: The invention described below relates to an enclosed cell sorting device and methods of using the device. The device is constructed so that the entire process of cell sorting can be conducted under fully anaerobic conditions to retain viability of anaerobic cells before, during, and after cell sorting. This is accomplished by creating an anaerobic atmosphere for the high speed cell sorter and all its components and by the use of airlocks that allow the introduction of anaerobic containers into the chamber containing the sample.

Abstract: An inflatable bioreactor bag for cell cultivation, which comprising a top and a bottom sheet of flexible material, joined together to form two end edges and two side edges, wherein one baffle or a plurality of baffles extend from the bottom sheet in a region where the shortest distance to any one of the two end edges is higher than about one fourth of the shortest distance between the two end edges.

Abstract: This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.

Abstract: A method of mixing a fluid includes positioning a flexible bag into the chamber of a support housing, the support housing having a plurality of baffles positioned so that the bag folds around the baffles. A fluid is delivered into a compartment of the flexible bag. A mixing element, such as an impeller, is moved within the compartment of the flexible bag so as to mix the fluid therein.

Abstract: An algae production reactor system comprising a reactor vessel which is disclosed. The system comprises (a) one or more liquid inlets and one or more liquid outlets; (b) one or more gas inlets at the bottom, the gas inlets being connected with a source of carbon dioxide, and one or more gas outlets at the top of the vessel; and (c) vertically interspaced and joined pairs of double glass plates which are at least partially submerged in the reactor liquid, the double glass plates having a layer of light-scattering particles in between and having a flat side being exposed to a light source, wherein part of the spaces between the double glass plates is arranged above the gas inlets for vertically circulating reactor liquid.

Abstract: A method and device for controlling a fermenting process, wherein the method comprises collecting samples of biological cells from a fermenting tank, obtaining the state information of the current biological cells based on the collected samples, comparing the state information of the current biological cells with preset target status information to obtain the difference between the state information of the current biological cells and preset target state information, and controlling the feed rate of nutritional solution into the fermenting tank. Real time control of biological fermenting process is thus accomplished based on the state information of the biological cells during fermentation, and the consistency during fermentation is improved. Complicated mathematical modeling and man-made data analysis are not required for the method and device.

Abstract: Techniques described herein are generally related to a biofilter for living spaces. The biofilter may include an organic biofilter medium to serve as a growth base for both plants and one or more microbial colonies. Example embodiments include systems, articles, methods and apparatus, as well as other embodiments that are described and claimed.

Abstract: A film-shaped tissue storage transport container and storage transport method. The storage transport container includes: an external container; a coupling mechanism which couples a container main body and a lid member of the external container; an internal container which is positioned within the external container; an interposed unit having elasticity and which is sandwiched between the internal container and the lid member; and a storage fluid with which the internal container is filled. A film-shaped tissue is present in a floating state in the storage fluid with which the internal container is filled.

Abstract: The present invention provides an apparatus and methods for producing tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA) and cannabichromenic acid (CBCA) in different ratios. The apparatus comprises:(i) a bioreactor comprising (a) an automated supply system configured to deliver a first automated supply of cannabigerolic acid (CBGA), a cannabinoid acid synthase, and a reaction mixture; and (b) a second automated system to cease the reaction; (ii) a controller configured to modify a property of the reaction mixture to produce the desired products; and (iii) an extractor configured to recover the tetrahydrocannabinolic acid (THCA), cannabichromenic acid (CBCA) or cannabidiolic acid (CBDA) and cannabichromenic acid.

Abstract: The present invention relates to an acoustic lysis system including a disposable cartridge that can be reversibly coupled to a platform having a small, high-frequency piezoelectric transducer array. In particular, the system releases viable DNA, RNA, and proteins from human or bacterial cells, without chemicals or additional processing, to enable high-speed sample preparation for clinical point-of-care medical diagnostics and use with nano/microfluidic cartridges. Also described herein are methods of making and using the system of the invention.

Abstract: The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.

Abstract: A self-contained circulation system, which supports the formation of capillaries in capillary growth sections and allows the formation of micro organoids and/or micro tissue sections for monitoring the effect of one or more test compounds and determining efficacy, side-effects, biosafety, metabolites, mode of action or organ regeneration as well as methods of establishing such micro organoids and/or micro tissue in the self-contained circulation system.

Abstract: A portable real-time heating and detection device includes a body, a cover and a detection unit. The body has an opening, and a base. The cover has a control unit and a fix unit. The detection unit is disposed on the base of the body and has a thermostat, an optical excitation, an optical detection, and a circuit board. The thermostat is disposed close to the opening and has at least one thermostat zone. The optical exciter is disposed between the thermostat and the base. The optical detector is disposed between the thermostat and the opening. The circuit board is electrical coupled to the control unit, the thermostat, the optical excitation, and the optical detector, respectively.

Abstract: A container assembly such as a petri dish or a contact plate for use as a sampling device for microorganisms includes a base member, a lid and a locking mechanism that provides a secure locking engagement between the lid and the base member. The locking mechanism is designed such that does not lock except upon application of a specific intentionally applied compressive force, and which may be readily disengaged from locking engagement without the need for a rotational movement or torsional force.

Abstract: Method of detection for droplet-based assays. In an exemplary method, an open end of a channel network may be placed into an emulsion. Droplets of the emulsion may be driven along a flow path from the open end, through a confluence region where a dilution fluid is introduced into the flow path from at least one dilution inlet channel to increase an average distance between droplets, and through an examination region disposed downstream of the confluence region. Light may be detected from the examination region as droplets pass through. The channel network may include a droplet inlet channel that meets the at least one dilution inlet channel at the confluence region. The droplet inlet channel may form a tapered region that is sized such that droplets leave the tapered region in single file.

Abstract: An object of the present invention is to provide an object-capturing device which accurately detects objects such as microorganisms captured at a test site. The object-capturing device of the present invention includes a capturing dish holding a carrier, which captures objects (microorganisms), on a first side of the capturing dish. The capturing dish has a through hole extending from the first side to a second side of the capturing dish. The object-capturing device is used in a way such that, after the objects are captured with the carrier directed upward, the carrier is directed downward and reagents for detecting the objects are contacted with the objects captured on the carrier through the through hole.

Abstract: A planter in the form of a tower planter having an internal vertical composting capability is provided. The planter has a hollow, vertical, outer cylindrical barrel with a plurality of openings formed about its cylindrical side. A hollow inner cylindrical composting tube having a plurality of perforations formed in its cylindrical wall is provided. The space formed between the inner tube and barrel wall is filled with growing medium. The inner tube is filled with compost material. A removable container is placed at the bottom of the tube and barrel, said container adapted to capture water draining out from the tube and barrel, as well as periodically receiving compost from the compost tube.

Abstract: A nucleic acid amplifier for carrying out a nucleic acid amplification reaction is disclosed. The amplifier includes a plurality of wells configured to carry out the nucleic acid amplification reaction. The amplifier also include a heating unit provided for every well. The amplifier further includes a light source for irradiating excitation light of a specified wavelength to all of the wells. A fluorescence detection unit is provided for every well.

Abstract: A device for amplifying a nucleic acid sample may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may include at least one heat pipe and a heat sink and the at least one heat pipe may include a first portion disposed proximate to the sample holder and a second portion disposed proximate to the heat sink.

Abstract: In order to enable the inside of a constant-temperature device to be sterilized using sterilization gas and prevent contamination during incubation, even when provided with an atmosphere measuring means having a CO2 sensor and an oxygen concentration sensor, a sensor unit (100) is arranged inside or close to an incubation chamber, and the internal atmosphere is measured by sucking-in the atmosphere of the incubation chamber (2) by means of an air-flow generation means (20). Furthermore, high-precision filters (23, 24) are arranged in the flow channel through which the atmosphere within the incubation chamber (2) is taken-in/discharged, preventing bacteria and/or cells from flowing in, and also preventing the diffusion of sterilization gas to the inside of a sensor unit (100) during sterilization.