Abstract: Various embodiments disclosed relate to a buffered aqueous solution. The buffered aqueous solution includes a buffer. The buffer includes a citrate component having a concentration in the solution in a range of from about 0.1 mM to about 600 mM. The buffer further includes a metal component having a concentration in the solution in a range of from about 0.01 mM to about 100 mM. The buffered aqueous solution has a pH in a range of from about 5 to about 9.

Abstract: The present invention relates to methods, devices and systems for associating consumable data with an assay consumable used in a biological assay. Provided are assay systems and associated consumables, wherein the assay system adjusts one or more steps of an assay protocol based on consumable data specific for that consumable. Various types of consumable data are described, as well as methods of using such data in the conduct of an assay by an assay system. The present invention also relates to consumables (e.g., kits and reagent containers), software, data deployable bundles, computer-readable media, loading carts, instruments, systems, and methods, for performing automated biological assays.

Abstract: This invention provides an HbA1c dehydrogenase that is capable of directly acting on hemoglobin A1c and is less likely to be influenced by oxygen concentration and a method for measurement and a kit of assay reagents using such HbA1c dehydrogenase. The HbA1c dehydrogenase having dehydrogenase activity and capable of directly acting on HbA1c is obtained by substitution of one or more amino acid residues at positions corresponding to positions 280, 269, 54, 241, and 267 of the amadoriase that is capable of directly acting on hemoglobin A1c and is derived from, for example, the genus Coniochaeta. This invention also provides a method for measurement of HbA1c, a kit of assay reagents, and a sensor using such HbA1c dehydrogenase. Such HbA1c dehydrogenase is capable of directly acting on hemoglobin A1c and has lowered oxidase activity and/or enhanced dehydrogenase activity.

Abstract: In one aspect, a method for detecting the labelling state of unknown species of molecules M contained in a sample exposed to a non-changing isotope labelling process using mass spectrometry is described.

Abstract: The present disclosure relates to the determination of pesticides, e.g., polar pesticides, in a sample using chromatography. The present disclosure can provide direct analysis of polar pesticides, including anionic polar pesticides, using high performance liquid chromatography. The polar pesticides are sufficiently retained and resolved to allow for multiple polar pesticide determinations in a single analysis.

Abstract: A method for manufacturing a test element for detecting at least one analyte in a body fluid, a test element for detecting at least one analyte in a body fluid, a method for detecting at least one analyte in a body fluid, a system for detecting at least one analyte in a body fluid and a method for manufacturing a test element for detecting at least one analyte in a body fluid are disclosed.

Abstract: A method and kit the simultaneous analysis of thyroid hormones (THs) and related metabolites in serum are disclosed. The method includes the extraction of THs and related metabolites, the derivatization of THs and related metabolites with 5-N-succinimidoxy-5-oxopentyl)triphenylphosphonium bromide (SPTPP), and the analysis of SPTPP derivatives using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Free or total THs (T3 and T4) together with their related metabolites (MIT, DIT, T0, T1, rT1, 3,3?-T2, 3,5-T2, and rT3) can be determined simultaneously, rapidly, and accurately. This invention overcomes the limitation that the traditional method can only be used for the analysis of T3 and T4 with low specificity. The invention exhibits extremely high analytical sensitivity for all of the target analytes (femtogram level).

Abstract: Disclosed herein is a method for quantifying phosphatidylethanol (“PEth”), a direct ethanol biomarker, from a blood sample using gas chromatography mass spectrometry. The method disclosed herein is useful for diagnosing acute and chronic alcohol abuse.

Abstract: The present disclosure provides a method of performing a functional assay on human spheroids, e.g., three-dimensional human cell spheroids using, in one embodiment, a fluorometric imaging plate reader.

Abstract: The disclosure provides a cell-based, label-free assay compatible with high-throughput screening (HTS) that can report quantitatively on enzyme activities by measuring mass changes of substrates with MALDI-mass spectrometry.

Abstract: In embodiments there is described a method for reducing false positives and negatives in the detection of SARS-CoV-2 in suspected patients using mass spectroscopy employing the steps of mixing samples of collected saliva and nasopharyngeal secretions in a single sample container; adding universal transport medium to the mixed samples in said single sample container; transporting the single sample container at a temperature above 0° C. to a remote location; deactivation of viral content of the mixed sample; protein digestion of the mixed sample; concomitant separation of peptides, ionization by mass spectroscopy of the separated peptides, and comparison of peptide patterns to known SARS-CoV-2 peptides. Also set forth in an embodiment is a collection container for collecting saliva and/or sputum, as well as a swab member, with universal transport medium and/or virus inactivating agent housed in separate compartment communicable with sample compartment through a one-way valve.

Abstract: A plurality of chromatograms is run using a salt gradient with a range of constant pH values or using a pH gradient with a range of constant salt concentrations. A chromatography optimization algorithm can be used to identify at least one salt gradient chromatogram or at least one pH gradient chromatogram to establish optimized buffer conditions. The chromatography optimization algorithm can include a total number of peaks algorithm, a peak-to-valley algorithm, and/or a peak capacity algorithm.

Abstract: The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.

Abstract: In one embodiment the present invention describes a test kit for detecting arsenic concentration in a sample. The test kit includes a stable bromide compound capable of being made into a solution comprising bromine, a dye, wherein the bromine to the dye ratio is in a range from about 4:1 to about 5:1, an acid, and a quantification system for detecting the concentration of arsenic in the sample. In another embodiment the present invention describes a test kit for detecting a concentration of up to 0.03 parts per million of arsenic in a sample. A method of using the test kit to detect concentration of arsenic in a sample is also described.

Abstract: A field effect transistor (FET) biosensor for virus detection of a selected virus within a sample volume is disclosed. The FET comprises a semiconductor substrate, a source and drain electrode on the substrate, the electrodes spaced to form a channel. A gate electrode carried on the substrate and located in the channel between the source and drain electrodes. An insulating layer is coupled to a top surface of the gate electrode and a bottom surface of the source and drain electrodes, with an open channel above the insulating layer. A channel material is coupled to the insulating layer. Aptamers are oriented within the open channel to bind to the channel material and with the selected virus to enable a detection of the selected virus by the FET biosensor based on a change in drain-source current at a selected gate voltage.

Abstract: The present invention relates to a chemical vapor-sensor bag with an integrated sensor array to verify the presence of specific chemical vapors inside a sealed bag. In an exemplary embodiment, a device can be sealed within a vapor-sensor bag to allow the device to be transported to and tested for contaminants at the point of use by an end user of the device. In another exemplary embodiment, a device can be coupled to a gas port on a vapor-sensor bag to allow gas within the device to be tested for contaminants. In another exemplary embodiment, gas from a device can be streamed through vapor-sensor bag by coupling the device to a first gas port on a vapor-sensor bag and allowing gas to exit the bag through a second gas port.

Abstract: Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.

Abstract: An environmental control system for use in a clinical analyzer module includes an upper deck environmental subsystem comprising a far field sensor, one or more heaters, one or more spine cooling fans, and one or more in-line fluid heat exchangers. The far field sensor is configured to acquire measurements of ambient temperature in the upper deck environmental subsystem. The heaters are configured to generate hot airflow based on the measurements of ambient temperature from the far field sensor. The spine cooling fans are configured to operate in a manner that mixes the hot airflow from the heaters with cool airflow based on the measurements of ambient temperature from the far field sensor. The in-line fluid heat exchangers are configured to heat fluids used in reactions performed on the clinical analyzer module to a constant temperature.

Abstract: Provided is a method for measuring a glycoprotein using an enzymatic method, and the method includes simplified steps.

Abstract: The present invention relates to a device of diagnosing a colorectal cancer and a method for providing colorectal cancer diagnosis information. A colorectal cancer diagnosing device according to the present invention includes: an input unit which acquires mass spectrometry data measured from a biological sample; a concentration detecting unit which measures a concentration of nudifloramide from the acquired mass spectrometry data; and a diagnosing unit which determines diagnosis information of the colorectal cancer based on a measurement result of the concentration detecting unit.