Patents Examined by Michael Paul Shimek
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Patent number: 11571636Abstract: The present disclosure relates to, inter alia, a method of quantitating an amount of an antibody molecule from a mixture comprising two or more antibody molecules, comprising separating each of the two or more antibody molecules from the mixture by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC) and quantitating an amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of any other antibody molecule in the mixture and either each antibody molecule is different from another antibody molecule in the mixture by more than about 0.25 unit on the Kyte & Doolittle hydropathy scale or each of the antibody molecules when nm alone on HIC-HPLC elutes at distinct run time with little overlap from the other antibody molecules in the mixture, or both.Type: GrantFiled: May 17, 2021Date of Patent: February 7, 2023Assignee: REGENERON PHARMACEUTICALS, INC.Inventors: Dingjiang Liu, Lin Luo, Long Xu
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Patent number: 11573236Abstract: A method for analyzing or detecting methimazole (“MTZ”) comprising contacting a sample suspected of containing MTZ with the dendrimer-stabilized silver nanoparticles and performing surface-enhanced Raman scattering (SERS). Graphene-dendrimer-stabilized silver nanoparticles (G-D-Ag).Type: GrantFiled: August 19, 2022Date of Patent: February 7, 2023Assignee: KING FAHD UNIVERSITY OF PETROLEUM AND MINERALSInventors: Tawfik A. Saleh, Mutasem M. Al-Shalafeh, Abdulaziz A. Al-Saadi
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Patent number: 11573237Abstract: A method for analyzing or detecting methimazole (“MTZ”) comprising contacting a sample suspected of containing MTZ with the dendrimer-stabilized silver nanoparticles and performing surface-enhanced Raman scattering (SERS). Graphene-dendrimer-stabilized silver nanoparticles (G-D-Ag).Type: GrantFiled: August 19, 2022Date of Patent: February 7, 2023Assignee: KING FAHD UNIVERSITY OF PETROLEUM AND MINERALSInventors: Tawfik A. Saleh, Mutasem M. Al-Shalafeh, Abdulaziz A. Al-Saadi
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Patent number: 11567046Abstract: The present invention is a data-processing device used for a chromatograph which continuously performs a series of analyses for components in each sample while sequentially introducing a plurality of samples into a column. The device includes: an input section configured to allow for input of information into a schedule table for a plurality of analyses, the schedule table describing an analysis condition including a combination of the values of a plurality of control parameters, the order of execution of the plurality of analyses, and information for identifying a sample to be subjected to each analysis; a chromatogram creating means configured to receive data sequentially collected during two or more analyses and create a joint chromatogram from the data if the two or more analyses have been continuously performed for the same sample according to the schedule table; and a display means configured to display the joint chromatogram.Type: GrantFiled: March 17, 2017Date of Patent: January 31, 2023Assignee: SHIMADZU CORPORATIONInventors: Futoshi Kurotobi, Toshikazu Minohata
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Patent number: 11567074Abstract: Provided herein are methods of identifying a protein capable of binding a ligand, the method comprising: (a) contacting the ligand with two or more samples comprising a plurality of proteins in a solution; (b) separating the proteins bound to the ligand (“bound proteins”) from the proteins that are not bound to the ligand (“unbound proteins”) in each sample; (c) denaturing and digesting the bound proteins to form a plurality of peptides in each sample; (d) quantifying a plurality of molecular features contained in the plurality of peptides in each sample, wherein the molecular features are defined as having a mass to charge ratio, retention time, and peak intensity as measured by mass spectrometry; and (e) ranking the molecular features that exhibit a statistically significant difference in quantity between the samples contacted with the ligand and a sample that is not contacted with the ligand (“statistically significant molecular feature”).Type: GrantFiled: September 25, 2017Date of Patent: January 31, 2023Assignee: University of Pittsburgh—Of the Commonwealth System of Higher EducationInventors: Nathan A. Yates, Steven James Mullett, Harris B. Bell-Temin, Andrey Bondarenko
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Patent number: 11555822Abstract: The invention relates to a method for preparation of a sample (10) of a formulation (11) for subsequent endotoxin determination, the formulation (11) suspected of comprising an endotoxin, the formulation (11) preferentially being a pharmaceutical formulation. The method comprises the following steps: application of the sample (10) to an endotoxin-free centrifugation column (2) containing a size exclusion chromatography matrix (5) that has been equilibrated with a suitable equilibration buffer (6) and elution of a flow through (15) of the sample by centrifugation, which flow through (15) can then be used for endotoxin determination. The equilibration buffer (6) is selected according to a subsequently used method of endotoxin determination, the equilibration buffer (6) only containing components not interfering with subsequently used method of endotoxin determination. Furthermore, the invention relates to a kit (20) for preparation of a sample (10).Type: GrantFiled: November 24, 2021Date of Patent: January 17, 2023Inventor: Andreas Buchberger
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Patent number: 11549954Abstract: The invention relates to the detection of non-metabolized vitamin D. In a particular aspect, the invention relates to methods for detecting underivatized non-metabolized vitamin D by mass spectrometry.Type: GrantFiled: March 21, 2022Date of Patent: January 10, 2023Assignee: Quest Diagnostics Investments IncorporatedInventors: Brett Holmquist, Nigel Clarke
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Patent number: 11543419Abstract: Provided are a measurement sample diluent that enables the measurement of the proportion of HbA1c in the total Hb molecules in a measurement sample (HbA1c (%)), with high sensitivity, high accuracy, and high correlation with HPLC without being affected by the length of time required to mix a measurement sample with the measurement sample diluent and to drop the mixture onto an immunochromatographic specimen for measuring HbA1c; and a kit containing the measurement sample diluent. The present invention relates to a measurement sample diluent for immunochromatography for quantifying the proportion of hemoglobin A1c in the total hemoglobin molecules in a measurement sample (hemoglobin A1c (%)); and the measurement sample diluent is an aqueous solution that contains a non-ionic surfactant, an anionic surfactant, and a buffer.Type: GrantFiled: December 27, 2018Date of Patent: January 3, 2023Assignee: TOYOBO CO., LTD.Inventors: Jun Okamoto, Keizo Yoneda
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Patent number: 11536732Abstract: Platforms for enzymatic assays for biomarkers, including systems, methods, and measuring devices by which a biomarker, such as creatinine, is measured using a small amount of biological fluid, such as blood, plasma, or serum. The measuring device or biosensor can be a test strip including a layered active component assembly positioned between two outer layers which enables multi-step enzymatic reactions operating in kinetic and/or endpoint (in which the reaction is allowed to near completion), and generally includes multiple layers with primary enzyme(s), coupling enzyme(s), and reagents to produce an optical signal correlated to the concentration of a biomarker in the sample. The test strip can be read using a portable optical reader coupled to a smart phone or tablet.Type: GrantFiled: March 13, 2020Date of Patent: December 27, 2022Assignee: Jana Care, Inc.Inventors: Daniel B. Lookadoo, Ashok A. Kumar, Jeremy E. Schonhorn, Christopher M. Uherek, Michal Depa
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Patent number: 11525815Abstract: The present invention discloses a method for product quality control and fingerprint detection of an epimedium brevicornu complex. The method uses high performance liquid chromatography, and can effectively realize the quality control of products containing traditional Chinese medicine components, and especially stable control of the quality of products containing a large quantity of non-traditional Chinese medicine components in formulas. Through step-by-step quality control, product quality fluctuation is reduced and stable quality is ensured. Meanwhile, the method is simple and convenient, does not need additional instruments and standards, saves the cost and is more conducive to actual production.Type: GrantFiled: April 13, 2020Date of Patent: December 13, 2022Inventors: Shengcan Zou, Li Li, Lei Zong, Jiancheng Zong, Shanglong Wang, Zengliang Zhang
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Patent number: 11525785Abstract: A method for detecting an analyte reactive towards luminol, comprising the steps of: feeding into a reaction chamber an alkaline solution of luminol, noble metal nanoparticles and at least one analyte reactive towards luminol, wherein the reaction chamber is in the form of a curved channel; detecting the light emitted due to a chemiluminescence reaction taking place in said channel; and discharging a reaction mass from said channel, characterized in that the average diameter of the metal nanoparticles is greater than 25 nm. Also provided is a microfluidic device for carrying out the method.Type: GrantFiled: June 25, 2017Date of Patent: December 13, 2022Assignee: B.G. NEGEV TECHNOLOGIES AND APPLICATIONS LTD., AT BEN-GURION UNIVERSITYInventor: Alina Karabchevsky
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Patent number: 11525166Abstract: Kits and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The kits and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The kits and methods utilize in various combinations, reversible inhibitors of kit components, thermolabile enzymes, poloxamers, various salts, indicators and one or more Loop-Mediated Isothermal Amplification (LAMP) primer sets for detecting single and/or multiple targets and variants of the targets including SARS-CoV-2 targets and variants thereof in a single reaction. The kits and methods permit detection of the target nucleic with similar sensitivity regardless of the presence of undefined mutations that may enhance the virulence of cells or viruses containing the undefined mutations.Type: GrantFiled: March 21, 2022Date of Patent: December 13, 2022Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Eric Hunt, Gregory Patton, Guoping Ren, Zhiru Li, Andrew Barry, Nicole Nichols, Catherine B. Poole, Harriet M. Strimpel, Ivan R. Correa, Jr., Clotilde Carlow, Esta Slayton
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Patent number: 11525814Abstract: A method for identifying converters from tobacco seedling population. The method includes: 1) sowing and cultivating tobacco seeds to be identified for 45-55 days; sampling a plurality of leaf disks from each of 45-55 days old seedlings; 2) incubating the plurality of leaf disks of each seedling in a sealed container at 37° C. for 10-12 hours, thereby obtaining a plurality of incubated tobacco leaves of each seedling; 3) immersing the plurality of incubated tobacco leaves of each seedling in an extractant, extracting alkaloids and obtaining an extract of each seedling; 4) analyzing the amounts of nicotine and nornicotine in the alkaloids extract of each seedling; and 5) automatically recognizing peaks of the alkaloids extract of each seedling, and calculating the percent nicotine conversion (PNC) and the pseudo percent nicotine conversion (PPNC).Type: GrantFiled: December 29, 2020Date of Patent: December 13, 2022Assignee: YUNNAN ACADEMY OF TOBACCO AGRICULTURAL SCIENCESInventors: Yong Li, Tao Pang, Xuejun Chen, Xueyi Sui, Junli Shi, Yongping Li, Guanghui Kong, Ping Chen, Yuping Wu
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Patent number: 11519890Abstract: Disclosed is a detection method for N-nitrosodimethylamine (NDMA) impurities, comprising: (1) obtaining a test solution containing a sample to be tested; and (2) detecting the test solution by means of gas chromatography-mass spectrometry to determine the content of an N-nitrosodimethylamine impurity in the sample. The method provided in the present invention has a good separating effect, a wide linear range, a high sensitivity and a good method durability, and can detect the content of N-nitrosodimethylamine (NDMA) in the sample rapidly and effectively.Type: GrantFiled: July 10, 2018Date of Patent: December 6, 2022Assignee: ZHEJIANG HUAHAI PHARMACEUTICAL CO., LTDInventors: Tan Xiao, Tianpei Huang, Jinsheng Lin, Qiang Zhou, Tong Wu, Danfeng Ye, Hong Cai, Wenquan Zhu, Wenbin Chen, Min Li
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Patent number: 11519917Abstract: Provided herein are compositions and methods for quantifying polyphosphates. In particular, provided herein are solution and substrate based assays for quantifying polyphosphates in complex samples.Type: GrantFiled: April 13, 2018Date of Patent: December 6, 2022Assignees: THE REGENTS OF THE UNIVERSITY OF MICHIGAN, THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOISInventors: James H. Morrissey, Stephanie A. Smith, Catherine J. Baker, Rachel Hemp, Richard J. Travers
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Patent number: 11499952Abstract: A process for quantifying the amount of unbound metal and bound metal in solution is provided. A process for quantifying the amount of bound metal amino acid chelate and free ligand in a solid (e.g., dry mixture such as an animal feed) is also provided.Type: GrantFiled: May 10, 2019Date of Patent: November 15, 2022Assignee: PREMEX, INC.Inventor: Paula Leon Monsalve
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Patent number: 11499980Abstract: The present invention provides a method, wherein the method classifies a subject as suffering from dry eye, the method consisting of: a. obtaining demographic data, consisting of the age and gender of the subject; b. obtaining a tear sample from the patient, and determining the level of human serum albumin; c. from the determined level of human serum albumin, assigning a score for the determined amount of human serum albumin; and d. from the assigned score, calculating a cutoff probability score, according to the following equation: exp ( - 0.6491 - 1.1142 * Albumin ) 1 + exp ( - 0.6491 - 1.1142 * Albumin ) wherein the subject has dry eye, if the calculated cutoff probability score is from 50% to 60%.Type: GrantFiled: January 3, 2020Date of Patent: November 15, 2022Assignee: Diagnostear, Ltd.Inventors: Amos Sommer, Ouriel Faktor, Eran Eilat
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Patent number: 11493486Abstract: A liquid chromatography reagent kit includes a mobile phase anti-adsorption concentrate. The mobile phase anti-adsorption concentrate includes a first solvent, a buffer, an acid-base regulating reagent, and an anti-adsorption reagent. A ratio of a molarity of the buffer to a molarity of the acid-base regulating reagent is 400:1 to 1:1. A ratio of the molarity of the buffer to a molarity of the anti-adsorption reagent is 100:1 to 1:2.Type: GrantFiled: January 6, 2021Date of Patent: November 8, 2022Assignee: MSonline Scientific Co., Ltd.Inventors: Chien-Pang Huang, Wei-Chun Huang
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Patent number: 11492673Abstract: Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.Type: GrantFiled: February 18, 2021Date of Patent: November 8, 2022Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Gregory Patton, Guoping Ren, Zhiru Li, Nicole Nichols
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Patent number: 11486867Abstract: An analysis device includes a main control circuit, a sub-control circuit, a backup execution part, and a restoration execution part. The main control circuit has a calibration information storage area for storing calibration information unique to the analysis device, and is configured to perform operation control unique to the analysis device using the calibration information. The sub-control circuit is communicable with the main control circuit and has a backup information storage area for storing the same information as the calibration information in the calibration information storage area. The backup execution part is configured to execute backup for storing information same as the calibration information stored in the calibration information storage area in the backup information storage area.Type: GrantFiled: April 23, 2020Date of Patent: November 1, 2022Assignee: Shimadzu CorporationInventor: Kazuya Kawakami