Abstract: Disclosed is a genetically recombinant Saccharomyces cerevisiae useful for degrading and utilizing kitchen wastes. Genes encoding ?-amylase (AMY), glucoamylase (GA) and acid protease (AP) were introduced into the genetically recombinant Saccharomyces cerevisiae using a Saccharomyces cerevisiae multi-gene co-expression vector and successfully expressed and secreted. The Saccharomyces cerevisiae so obtained are capable of secreting amylases and protease to degrade the starch and proteins in kitchen wastes to produce carbon and nitrogen sources such as glucose, polypeptides and amino acids, allowing fermentation into ethanol.
Abstract: A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.
Type:
Grant
Filed:
February 7, 2017
Date of Patent:
February 25, 2020
Assignee:
President and Fellows of Harvard College
Inventors:
Marc J. Lajoie, Christopher J. Gregg, Joshua A. Mosberg, George M. Church
Abstract: Nucleic acids encoding mutant elongation factor proteins (EF-Sep), phosphoseryl-tRNA synthetase (SepRS), and phosphoseryl-tRNA (tRNASep) and methods of use in site specific incorporation of phosphoserine into a protein or polypeptide are described. Mutant EF-Sep proteins are disclosed that bind Sep-tRNASep and protect Sep-tRNASep from deacylation. In a preferred embodiment the nucleic acids are on vectors and are expressed in cells such as bacterial cells, archeaebacterial cells, and eukaryotic cells. Proteins or polypeptides containing phosphoserine produced by the methods described herein can be used for a variety of applications such as research, antibody production, protein array manufacture and development of cell-based screens for new drug discovery.
Abstract: The present invention relates to methods and compositions for determination of and uses of specific methylation patterns indicative of adenoma and carcinoma. In particular, the invention relates to analysis of defined CpG loci that are coordinately methylated in DNAs from cancer and adenoma samples, methods for identifying coordinately methylated loci, and methods of using analysis of coordinately methylated loci in one or more marker regions in the design of assays for adenoma and cancer.
Type:
Grant
Filed:
September 28, 2016
Date of Patent:
December 31, 2019
Assignee:
EXACT SCIENCES DEVELOPMENT COMPANY, LLC
Inventors:
David A. Ahlquist, William R. Taylor, Hongzhi Zou, Graham P. Lidgard
Abstract: The present invention relates generally to the field of RNA Control Devices and/or destabilizing elements (DE) combined with Chimeric Antigen Receptors (CARs) in eukaryotic cells. The present invention also relates to split CARs (Side-CARs) in eukaryotic cells. More specifically, the present invention relates to DEs, RNA Control Devices, and/or side-CARs combined with Chimeric Antigen Receptors to make small molecule actuatable CAR polypeptides. The present invention also relates to DE-CARs, Smart CARs (Smart=small molecule actuatable RNA trigger), Smart-DE-CARs, and/or Side-CARs for use in the treatment of disease.
Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
Type:
Grant
Filed:
February 16, 2017
Date of Patent:
November 12, 2019
Assignee:
Geneweave Biosciences, Inc.
Inventors:
Diego Ariel Rey, Marc Rehfuss, Xiaowen Liu
Abstract: A nanosensor for detecting and quantifying lactate in different types of samples, such as tissues, intra-cellular and subcellular compartments, with high spatial and temporal resolution is disclosed. Methods comprising use of the nanosensor for quantifying the activity of lactate transporters, rates of cellular lactate production and cellular lactate consumption, and rate of mitochondrial pyruvate consumption are also disclosed. Methods for quantifying the transformation in energy metabolism that characterizes cancer cells with single-cell resolution and for detecting interference of candidate drugs with mitochondrial energetics are additionally disclosed.
Type:
Grant
Filed:
April 13, 2012
Date of Patent:
November 5, 2019
Assignees:
CENTRO DE ESTUDIOS CIENTIFICOS DE VALDIVIA, CARNEGIE INSTITUTION OF WASHINGTON
Inventors:
Luis Felipe Barros Olmedo, Alejandro San Martin, Sebastian Ceballo Charpentier, Wolf B. Frommer
Abstract: The invention relates to an isolated nucleic acid sequence comprising a promoter, which is a native sequence of Pichia pastoris comprising the nucleic acid sequence of pCS1 of SEQ ID 1, or a functionally active variant thereof which is a size variant, a mutant or hybrid of SEQ ID 1, or a combination thereof, expression constructs and recombinant host cells comprising the promoter, and a method of producing a protein of interest under the control of the promoter. It further relates to a method to identify a constitutive promoter from eukaryotic cells, and an isolated nucleic acid sequence comprising a promoter which when operatively linked to a nucleotide sequence encoding a protein of interest directs the expression thereof in a host cell at an expression level that is higher than under control of the native pGAP promoter at high and low growth rates.
Type:
Grant
Filed:
December 18, 2013
Date of Patent:
October 1, 2019
Assignee:
Lonza Ltd
Inventors:
Diethard Mattanovich, Brigitte Gasser, Roland Prielhofer
Abstract: A non-natural modified CMV promoter is provided. Such a promoter can include a promoter nucleotide sequence that is at least 80% homologous to a sequence selected from the group consisting of SEQ ID 01, SEQ ID 02, SEQ ID 03, SEQ ID 04, SEQ ID 05, SEQ ID 06, SEQ ID 07, and compliments thereof.
Type:
Grant
Filed:
January 24, 2017
Date of Patent:
July 23, 2019
Assignee:
University of Utah Research Foundation
Inventors:
Wesley I. Sundquist, Eiji Morita, Jun Arii
Abstract: Disclosed are methods of predicting responsiveness of a cancer cell to a tyrosine kinase inhibitor, and methods of predicting the risk of progression of a cancer cell to a more aggressive form. Also provided are methods of reducing proliferation or promoting differentiation of a cancer cell having reduced level of Numb or increased level of Msi. Further disclosed are methods of treating a mammalian subject having cancer and methods of assessing an agent for chemotherapeutic potential.
Type:
Grant
Filed:
July 1, 2015
Date of Patent:
July 9, 2019
Assignee:
Duke University
Inventors:
Tannishtha Reya, Hyog Y. Kwon, Takahiro Ito, Dong-Wook Kim, Vivian Oehler, Jerald Radich
Abstract: A fusion polynucleotide including a murine CMV promoter and an intron, a recombinant vector including the fusion polynucleotide and a gene encoding a polypeptide of interest, a recombinant cell including the recombinant vector, and a method of producing a polypeptide of interest using the recombinant vector and/or the recombinant cell.
Type:
Grant
Filed:
December 7, 2015
Date of Patent:
July 2, 2019
Assignee:
SAMSUNG BIOEPIS CO., LTD.
Inventors:
Su Jeong Hwang, Min-Kyung Kim, Sangjoon Park, Chanmoo Lee, Mi Young Cho
Abstract: Provided is an expression vector having an improved ability to express a gene. Also provided are cells transformed by the expression vector and a method for mass-producing a target protein by using the cells. The expression vector contains a simian virus 40 promoter, a scaffold attachment region or matrix attachment region element, and a chimeric intron. The vector shows an improved ability to express a gene, and thus, can attain a significantly increased expression of a heterogeneous gene.
Type:
Grant
Filed:
December 29, 2014
Date of Patent:
May 28, 2019
Assignee:
MOGAM BIOTECHNOLOGY INSTITUTE
Inventors:
Jung-Seob Kim, Seongtae Yun, Heechun Kwak, Mee Sook Oh, Sumin Lee
Abstract: The present techniques provides methods for use in conjunction with an article of manufacture having a plurality of sample sites of an array and to identify sample sites based on image data. The array may be divided into a series of domains with each domain having patterns of sample sites. Neighboring domains may be oriented at different angles such that each domain does not have the same pattern orientation.
Abstract: The present invention features recombinant adeno-associated vectors for delivery of genes to both skeletal and cardiac muscle and methods for treatment of muscle defects, including Duchenne's muscular dystrophy.
Type:
Grant
Filed:
July 26, 2014
Date of Patent:
May 14, 2019
Assignee:
CONSIGLIO NAZIONALE DELLE RICERCHE
Inventors:
Claudio Passananti, Nicoletta Corbi, Maria Grazia Di Certo, Elisabetta Mattei, Cinzia Pisani, Georgios Strimpakos
Abstract: An object of the present invention is to effectively induce cancer cell apoptosis using the anti-TRAIL-R1 antibody(ies) and the anti-TRAIL-R2 antibody(ies) and to reduce the toxicity imposed on normal cells. The present invention relates to recombinant obligate anaerobic Gram-positive bacteria that include a nucleic acid encoding a fusion protein having 3 or more anti-TRAIL-R1 single-chain antibodies and/or 3 or more anti-TRAIL-R2 single-chain antibodies, in an expressible state.
Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
Type:
Grant
Filed:
July 13, 2017
Date of Patent:
March 12, 2019
Assignee:
GeneWeave Biosciences, Inc.
Inventors:
Diego Ariel Rey, Nikol de Forest, Heather Cox, Soni Shukla
Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
Type:
Grant
Filed:
July 28, 2017
Date of Patent:
March 12, 2019
Assignee:
GeneWeave Biosciences, Inc.
Inventors:
Diego Ariel Rey, Nikol de Forest, Heather Cox, Soni Shukla
Abstract: The invention discloses a promoter which can be induced to express in acidic conditions, and relates to the field of bioengineering technology. The promoters of the invention are separated from A. niger and can actuate and/or regulate the expression of the effectively connected nucleic acids in A. niger. In the invention the expression of the promoters is studied in A. niger, and it is indicated that some promoters show weak expression, and some show strong activity. The invention provides an effective method and new thought for organic acids production by fungi or other products produced by fermentation under acidic conditions.
Type:
Grant
Filed:
February 8, 2018
Date of Patent:
March 5, 2019
Assignee:
Jiangnan University
Inventors:
Long Liu, Jian Chen, Guocheng Du, Xian Yin, Jianghua Li
Abstract: The present invention provides a plasmid, method and kit for producing heat labile enterotoxin B-subunit based on a Bacillus subtilis expression system. By comparing with the conventional method in the art, the present invention has the advantages of high safety, good yield, and simplified process and is therefore favorable for the commercialization object of heat labile enterotoxin B-subunit in the application of vaccine adjuvant.
Type:
Grant
Filed:
December 16, 2013
Date of Patent:
February 19, 2019
Assignee:
AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE
Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
Type:
Grant
Filed:
April 19, 2013
Date of Patent:
February 12, 2019
Assignee:
Cepheid
Inventors:
Russell Higuchi, Stacey Ellen Wallace, Edwin Wei-Lung Lai