Patents Examined by Mindy G Brown
  • Non-replicative transduction particles and transduction particle-based reporter systems
    Patent number: 9752200
    Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
    Type: Grant
    Filed: June 15, 2016
    Date of Patent: September 5, 2017
    Assignee: Geneweave Biosciences, Inc.
    Inventors: Diego Ariel Rey, Nikol de Forest, Heather Cox, Soni Shukla
  • High-throughput DNA fragment assembly
    Patent number: 9752154
    Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.
    Type: Grant
    Filed: July 23, 2013
    Date of Patent: September 5, 2017
    Assignee: Dow AgroSciences LLC
    Inventors: Sandeep Kumar, Steven L. Evans, Manju Gupta
  • Genetic markers of mental illness
    Patent number: 9738934
    Abstract: This invention relates to genetic markers of mental illness, e.g., schizophrenia (SZ), and methods of use thereof.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: August 22, 2017
    Assignee: Clinical Reference Laboratory, Inc.
    Inventors: Mark David Brennan, Timothy Lynn Ramsey
  • Filamentous fungi having an altered viscosity phenotype
    Patent number: 9725727
    Abstract: Described are compositions and methods relating to variant filamentous fungi having altered growth characteristics. Such variants are well-suited for growth in submerged cultures, e.g., for the large-scale production of enzymes and other proteins for commercial applications.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: August 8, 2017
    Assignee: DANISCO US INC
    Inventors: Elizabeth A. Bodie, Robert James Pratt, II
  • HiC: method of identifying interactions between genomic loci
    Patent number: 9708648
    Abstract: The disclosed Hi-C protocol can identify genomic loci that are spatially co-located in vivo. These spatial co-locations may include, but are not limited to, intrachromosomal interactions and/or interchromosomal interactions. Hi-C techniques may be applied to many different scales of interest. For example, on a large scale, Hi-C techniques can be used to identify long-range interactions between distant genomic loci.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: July 18, 2017
    Assignees: University of Massachusetts, Massachusetts Institute of Technology, President and Fellows of Harvard College, Whitehead Institute for Biomedical Research
    Inventors: Job Dekker, Erez Lieberman Aiden, Nynke Van Berkum, Andreas Gnirke, Eric Lander, Chad Nusbaum, Louise Williams, Alexandre Melnikov, Georgia Giannoukos
  • Method of anaerobic tissue-targeted gene expression initiated by alcohol dehydrogenase promoter and the application thereof
    Patent number: 9708617
    Abstract: A proteomic screening method for anaerobic-specific and expression-effective promoter, and a method of specially delivering and selectively stably expressing target gene in anaerobic tissue by an alcohol dehydrogenase promoter and uses thereof. The latter comprises an anaerobically-induced alcohol dehydrogenase promoter which is used as target gene promoter, anaerobic target bacteria and low copy number plasmid. Therefore, the target gene can be specially and highly expressed under hypoxia condition in vivo or in vitro. The selective gene expression which is driven by the alcohol dehydrogenase promoter in anaerobic tissue can be used as gene therapy method to treat anaerobic tissue disease including tumor, or to prepare anti-tumor drug.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: July 18, 2017
    Assignee: NANJING UNIVERSITY
    Inventors: Zichun Hua, Jianxiang Chen, Dongping Wei, Hongqin Zhuang
  • Filamentous fungal host strains and DNA constructs, and methods of use thereof
    Patent number: 9701969
    Abstract: The present disclosure relates to filamentous fungal host strains and recombinant DNA constructs for creation and use thereof. The filamentous fungal host strains are particularly useful for efficiently screening DNA libraries encoding recombinant proteins.
    Type: Grant
    Filed: June 3, 2011
    Date of Patent: July 11, 2017
    Assignee: DANISCO US INC.
    Inventors: Benjamin S. Bower, Thijs Kaper, Bradley R. Kelemen
  • Promoters for expressing genes in a fungal cell
    Patent number: 9701970
    Abstract: The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.
    Type: Grant
    Filed: February 3, 2016
    Date of Patent: July 11, 2017
    Assignee: Novozymes, Inc.
    Inventors: Sandra Merino, Debbie Yaver
  • Polynucleotides comprising a reporter sequence operatively linked to a regulatory element
    Patent number: 9695481
    Abstract: The invention provides a polynucleotide comprising a reporter sequence operatively linked to a regulatory element of a gene selected from Bscl2, Srxnl, Cbr3, Ephxl, Nope, Cdknla, Perp, Pltp, Cgrefl, Ltb4r1, Btg2, Gpx2, Ltb4r2, Ddit4l, Fosl1, and Egr1, which regulatory element stimulates expression of the reporter sequence in response to a genotoxic agent or to an oxidative stress-inducing agent. The invention also provides a method of detecting a genotoxic or oxidative stress-inducing agent comprising subjecting a cell containing the polynucleotide of the invention to a test agent; and assessing the expression of the reporter sequence.
    Type: Grant
    Filed: September 15, 2011
    Date of Patent: July 4, 2017
    Assignees: UNIVERSITEIT LEIDEN, ACADEMISCH ZIEKENHUIS LEIDEN
    Inventors: Bob Van de Water, Harm Vrieling
  • Methods of introducing nucleic acids into cellular DNA
    Patent number: 9688994
    Abstract: A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.
    Type: Grant
    Filed: July 30, 2013
    Date of Patent: June 27, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Marc J. Lajoie, Christopher J. Gregg, Joshua A. Mosberg, George M. Church
  • Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
    Patent number: 9689012
    Abstract: The present invention relates to methods of assembling a plurality of genetic units to form synthetic genetic constructs. This method involves appending universal adapter oligonucleotides and flexible adapter oligonucleotides to the 5? and 3? ends of separate genetic units to be assembled to form separate dual extended genetic units. The dual extended genetic units are assembled together via homologous recombination between the flexible adapter oligonucleotide portions of the dual extended units to form synthetic genetic constructs. The present invention further relates to synthetic genetic constructs formed using the methods of the present invention, and vectors, cells, and organisms containing such synthetic genetic constructs.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: June 27, 2017
    Assignee: Cornell University
    Inventors: Sébastien Cunnac, Alan Collmer
  • Nucleic acid element for use in analysis, and analytical method, analytical reagent, and analytical instrument using same
    Patent number: 9689025
    Abstract: The technique by which simple analysis of an intended subject to be analyzed can be carried out is provided. In this technique, a nucleic acid element 16 for use in analysis including: a first nucleic acid part 12; and a second nucleic acid part 13 is used. In the nucleic acid element 16, the first nucleic acid part 12 is a binding part that can bind to a subject 11 to be analyzed, and the second nucleic acid part 13 is a labeling part that can distinguish between binding and non-binding of the first nucleic acid part 12 to the subject 11. It is preferred that the first nucleic acid part 12 is an aptamer against the subject 11. The subject 11 can be analyzed easily by using the nucleic acid element 16, binding the subject 11 to the first nucleic acid part 12, and then analyzing the binding with the second nucleic acid part 13.
    Type: Grant
    Filed: August 6, 2010
    Date of Patent: June 27, 2017
    Assignee: NEC Solution Innovators, Ltd.
    Inventors: Iwao Waga, Jou Akitomi, Makio Furuichi
  • Expression process
    Patent number: 9677104
    Abstract: A process for the production of a target recombinant polypeptide is provided. The process comprises expressing a vector comprising a promoter operatively linked to an expression cassette for the target recombinant polypeptide; and a perfect palindrome operator sequence in a culture medium, wherein the culture medium is substantially free from antibiotic.
    Type: Grant
    Filed: December 10, 2011
    Date of Patent: June 13, 2017
    Assignee: Fujifilm Diosynth Biotechnologies UK Limited
    Inventors: Ian John Hodgson, Tibor Nagy
  • Single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution
    Patent number: 9663833
    Abstract: The invention relates to a double-stranded polynucleotide comprising on its positive strand considered from its 5? end to its 3? end, (i) a promoter of a gene of interest or several promoters of various genes of interest selected among genes which are endogenous to a determined cell, and, (ii) one or several barcode(s) wherein each barcode contains at least one barcode unit formed of at least one, especially of multiple, recognition binding sites each binding site being composed of a nucleotide sequence, and wherein each of said barcode(s) is under the control of at least one of said promoter(s) for transcription. It further concerns use of said polynucleotide to monitor gene expression patterns in living cells, especially in single cells, with a rapid and high spatio-temporal resolution.
    Type: Grant
    Filed: September 12, 2008
    Date of Patent: May 30, 2017
    Assignee: INSTITUT PASTEUR
    Inventors: Musa Mhlanga, Jost Enninga, Philippe Sansonetti, Ulf Nehrbass
  • Optimized expression cassette for expressing a polypeptide with high yield
    Patent number: 9663797
    Abstract: The present invention is based on the finding that the combination of a specific 5?UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.
    Type: Grant
    Filed: November 18, 2013
    Date of Patent: May 30, 2017
    Assignee: NOVARTIS AG
    Inventors: Zorica Dragic, Sabine Geisse, Rita Schmitz, Burkhard Wilms
  • Polynucleotides having leader sequence function
    Patent number: 9657300
    Abstract: The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: May 23, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi
  • Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
    Patent number: 9650630
    Abstract: The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences from the promoter region of a 60S ribosomal protein L32 gene (RPL32), for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: May 16, 2017
    Assignee: CellaGenics B.V.
    Inventors: Arie Pieter Otte, Femke Hoeksema, John Antonius Verhees, Henricus Johannes Maria Van Blokland
  • Method for detecting analytes
    Patent number: 9643180
    Abstract: The invention provides an improved method for sensitive and specific detection of target molecules, cells, or viruses. The inventive method uses large area imaging to detect individual labeled targets complexed with a target-specific selection moiety. The invention eliminates wash steps through the use of target-specific selection through one or more liquid layers that can contain optical dye and density agents. By eliminating washes the invention simplifies instrumentation engineering and minimizes user steps and costs. The invention uses sensitive image analysis to enumerate individual targets in a large area, is scalable, and can be deployed in systems ranging in complexity from manual to highly automated.
    Type: Grant
    Filed: September 24, 2009
    Date of Patent: May 9, 2017
    Assignee: First Light Biosciences, Inc.
    Inventors: Ezra Abrams, Sadanand Gite, Lisa Shinefeld, Don Straus, Gordon Siek, Greg Yantz
  • Digital sequence analysis of DNA methylation
    Patent number: 9637792
    Abstract: The present invention relates to methods and compositions for determination of and uses of specific methylation patterns indicative of adenoma and carcinoma. In particular, the invention relates to analysis of defined CpG loci that are coordinately methylated in DNAs from cancer and adenoma samples, methods for identifying coordinately methylated loci, and methods of using analysis of coordinately methylated loci in one or more marker regions in the design of assays for adenoma and cancer.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: May 2, 2017
    Assignees: MAYO FOUNDATION FOR MEDICAL EDUCATION AND RESEARCH, EXACT SCIENCES CORPORATION
    Inventors: David A. Ahlquist, William R. Taylor, Hongzhi Zou, Graham P. Lidgard
  • Identification and characterization of the spinactin biosysnthesis gene cluster from spinosyn producing
    Patent number: 9631195
    Abstract: This disclosure concerns methods for producing a spinosyn producing host cell that comprises modifying a nucleic acid molecule encoding for spinactin by introducing, mutating, deleting, replacing or inactivating a nucleic acid sequence encoding one or more activities of a polypeptide encoded by the nucleic acid molecule. Methods for producing a modified Saccharopolyspora spinosa organism or strain are also disclosed.
    Type: Grant
    Filed: December 13, 2012
    Date of Patent: April 25, 2017
    Assignee: Dow AgroSciences LLC
    Inventors: Nadaraj Palaniappan, Paul Lewer, Paul Richard Graupner