Patents Examined by N C Yang
  • Patent number: 8759008
    Abstract: The present invention provides a composite material including a substrate having an oxide surface, and, a continuous monolayer on the oxide surface, the monolayer including a silicon atom from a trifunctional alkyl/alkenyl/alkynyl silane group that attaches to the oxide surface, an alkyl/alkenyl/alkynyl portion of at least three carbon atoms, a polyalkylene glycol spacer group, and either a reactive site (e.g., a recognition ligand) or a site resistant to non-specific binding (e.g., a methoxy or the like) at the terminus of each modified SAM. The present invention further provides a sensor element, a sensor array and a method of sensing, each employing the composite material. Patterning is also provided together with backfilling to minimize non-specific binding.
    Type: Grant
    Filed: May 11, 2010
    Date of Patent: June 24, 2014
    Assignee: Los Alamos National Security, LLC
    Inventors: Basil I. Swanson, Aaron S. Anderson, Andrew M. Dattelbaum, Jurgen G. Schmidt
  • Patent number: 8652564
    Abstract: Protein binding onto a polymeric surface is enhanced by treating the surface with an amine-bearing polymer like poly(ethylene imine). When used in ELISA, the treated surface improves the performance of the assay.
    Type: Grant
    Filed: May 7, 2007
    Date of Patent: February 18, 2014
    Assignee: The Ohio State University Research Foundation
    Inventors: Shang-Tian Yang, Yunling Bai
  • Patent number: 8642275
    Abstract: The use of a bacterial antigen obtained from bacterial cells in the production of antibodies for immunological assays for detecting bacterial cells.
    Type: Grant
    Filed: September 16, 2008
    Date of Patent: February 4, 2014
    Assignee: Instytut Hodowli i Aklimatyzacji Roslin—Panstwowy Instytut Badawczy
    Inventor: Wlodzimierz Przewodowski
  • Patent number: 8628951
    Abstract: Provided are a lab-on-a-chip and a method of driving the same. The lab-on-a-chip includes a first region where a lower substrate and an upper substrate are bonded to each other, a second region where the lower and upper substrates are not bonded to each other, a gap control member disposed at a terminal of the second region facing an interface between the first and second regions, and configured to control a gap between the lower and upper substrates at the terminal of the second region, and a pressure application member disposed at the terminal of the second region facing the interface between the first and second regions, and configured to apply pressure to the upper substrate at the terminal of the second region to reduce a gap between the lower and upper substrates in the center of the second region. Thus, binding events between a fluid sample to be analyzed and a reagent can be maximized so that a high signal can be obtained using only an infinitesimal quantity of sample.
    Type: Grant
    Filed: September 29, 2010
    Date of Patent: January 14, 2014
    Assignee: Electronics and Telecommunications Research Institute
    Inventor: Hye Yoon Kim
  • Patent number: 8623596
    Abstract: The present invention provides a method and device for detecting and quantifying the concentration of magnetic-responsive micro-beads dispersed in a liquid sample. Also provided is a method and microfluidic immunoassay pScreen™ device for detecting and quantifying the concentration of an analyte in a sample medium by using antigen-specific antibody-coated magnetic-responsive micro-beads. The methods and devices of the present invention have broad applications for point-of-care diagnostics by allowing quantification of a large variety of analytes, such as proteins, protein fragments, antigens, antibodies, antibody fragments, peptides, RNA, RNA fragments, functionalized magnetic micro-beads specific to CD4+, CD8+ cells, malaria-infected red blood cells, cancer cells, cancer biomarkers such as prostate specific antigen and other cancer biomarkers, viruses, bacteria, and other pathogenic agents, with the sensitivity, specificity and accuracy of bench-top laboratory-based assays.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: January 7, 2014
    Assignee: Carnegie Mellon University
    Inventors: Alberto Gandini, James F. Antaki
  • Patent number: 8609435
    Abstract: A method for capillary electrophoretic analysis of a biological sample, comprising using negatively surcharged modified antibodies so that they migrate to a zone located outside the migration zone for proteins from the biological sample when they are separated during electrophoresis is disclosed. The antibodies have antigenic specificity for a predetermined target protein.
    Type: Grant
    Filed: November 9, 2004
    Date of Patent: December 17, 2013
    Assignee: SEBIA
    Inventors: Frédéric Robert, Régis Andre
  • Patent number: 8585972
    Abstract: One object of the present invention is to provide a biosensor and a production method therefor, by which hydrogel that enables immobilization of a physiologically active substance can be conveniently produced using safe raw materials. The present invention provides a biosensor which comprises a substrate having a metal layer on its surface, wherein a hydrophilic polymer having a reactive functional group capable of reacting with a hydroxyl group or an amino group of a physiologically active substance is bound to the metal layer directly or indirectly via an intermediate layer.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: November 19, 2013
    Assignee: FUJIFILM Corporation
    Inventors: Taisei Nishimi, Toshihide Ezoe, Toshiaki Kubo, Hidetoshi Tomita
  • Patent number: 8568659
    Abstract: The present invention is directed to a fiber optic device consisting of a fiber bundle having multiple legs of silica fibers, using a plurality of microspheres construct to attach target cells, for the assay of cytotoxic compounds. Each leg of silica fibers consists of twenty-five or more silica fibers treated with biotin and streptavidin treated microspheres which chemically bind the microspheres to the silica fibers. Further, the present invention is directed to the unique microspheres. The microspheres have a core, preferably alginate, with an outer surface of chitosan. The present invention is further directed to the use of the described fiber optic device to isolate, research and develop biological medicaments and diagnostic cytotoxic compounds. The fiber optic device utilizes thousands of fibers and the unique microspheres to provide a high-throughput screening device.
    Type: Grant
    Filed: September 11, 2008
    Date of Patent: October 29, 2013
    Assignee: Mabgen, LLC
    Inventor: Dennis Lee
  • Patent number: 8563260
    Abstract: A capillary for an immunoassay is provided which comprises an insoluble layer of an oxidase formed on an inner wall surface of said capillary, said oxidase being conjugated to a first antibody, and a layer of a hydrophilic polymer formed on said insoluble layer, said hydrophilic polymer layer containing a second antibody conjugated to a peroxidase, wherein said first and second antibodies are capable of binding to the same antigen.
    Type: Grant
    Filed: December 7, 2009
    Date of Patent: October 22, 2013
    Assignee: Osaka Prefecture University Publication Corpoaration
    Inventors: Hideaki Hisamoto, Terence Gaba Henares
  • Patent number: 8557605
    Abstract: The invention relates to a device for performing immunological, histochemical and cytochemical, molecular biological, enzymological, clinical-chemical and other analyzes, wherein the device comprises an object holder having one or more elongate adhesive surfaces and a reagent holder having one or several channels. The object holder is detachably connectable to the reagent holder in such a manner that the elongate adhesive surfaces each face one of the channels and, when reaction partners bound to a solid phase are disposed on the elongate adhesive surfaces and reactants dissolved in liquid are present in the channels, the reaction partners and the reactants are in contact. Means are provided for preventing the liquid from passing from one channel into an adjacent channel.
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: October 15, 2013
    Assignee: Euroimmun Medizinische Labordiagnostika AG
    Inventors: Winfried Stöcker, Martin Rateike, Bianca Maltzahn, Rasmus Behring
  • Patent number: 8556821
    Abstract: A system for processing oscillometric data from a plurality of pressure steps to determine the blood pressure of a patient as disclosed herein. A heart rate monitor connected to the patient acquires the patient's heart rate. A time to frequency domain converter receives oscillometric data and converts the oscillometric data into the frequency domain. A harmonic frequency calculator is connected to the heart rate monitor and derives at least the heart rate fundamental frequency. A filter connected to the time to frequency domain converter and the harmonic frequency calculator that produces a filter frequency domain oscillometric signal. A reconstruction calculator receives the filtered frequency domain oscillometric signal and reconstructs a time domain oscillometric signal. A method of computing an oscillometric envelope for use in determining the blood pressure of a patient is also disclosed herein.
    Type: Grant
    Filed: February 20, 2008
    Date of Patent: October 15, 2013
    Assignee: General Electric Company
    Inventors: Lawrence T. Hersh, Sai Kolluri, Robert F. Donehoo
  • Patent number: 8551406
    Abstract: Methods for identifying a biological particle in a sample medium include generating an Electrophoretic Quasi-elastic Light Scattering (EQELS) spectrum for the biological particle in the sample medium. The EQELS spectrum is compared to a reference database comprising a plurality of spectra, and each of the plurality of spectra correspond to an EQELS spectrum for one of a plurality of known biological particles. The biological particle in the sample medium is identified from the comparison.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: October 8, 2013
    Assignee: The University of North Carolina at Chapel Hill
    Inventor: Don A. Gabriel
  • Patent number: 8551405
    Abstract: Methods for identifying a biological particle in a sample medium include generating an Electrophoretic Quasi-elastic Light Scattering (EQELS) spectrum for the biological particle in the sample medium. The EQELS spectrum is compared to a reference database comprising a plurality of spectra, and each of the plurality of spectra correspond to an EQELS spectrum for one of a plurality of known biological particles. The biological particle in the sample medium is identified from the comparison.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: October 8, 2013
    Assignee: The University of North Carolina at Chapel Hill
    Inventor: Don A. Gabriel
  • Patent number: 8551698
    Abstract: A method comprising loading a sample into a microfluidic device which comprises plural sample chambers, subdividing the sample into a plurality of sample portions, such that respective sample portions are positioned in each of a plurality of the sample chambers, and subjecting the sample portions loaded into the respective sample chambers to at least a first amplification step. Each of the sample chambers has a respective volume such that if a sample portion positioned in the sample chamber comprises at least one molecule of a target nucleic acid, the target nucleic acid would attain a detectable concentration in the sample chamber after a single round of amplification.
    Type: Grant
    Filed: August 13, 2007
    Date of Patent: October 8, 2013
    Assignees: Applied Biosystems, LLC, The United States Department of Health and Human Services
    Inventors: James F. Brown, Jonathan E. Silver
  • Patent number: 8524445
    Abstract: The present invention discloses microwave resonant absorption (MRA) of viruses through dipolar coupling to viral confined acoustic modes. The unique geometrical and mechanical properties of viruses can be reflected by the MRA frequencies, MRA linewidth, and the absorption selection rules of high-order acoustic modes. Combined these spectral characteristics with the mature microwave technology, this invention provides a novel physical mechanism to develop non-affinity-based rapid detection and identification of viruses. It can be applied in preliminary characterization on unknown or mutant viruses.
    Type: Grant
    Filed: March 18, 2008
    Date of Patent: September 3, 2013
    Assignee: National Taiwan University
    Inventors: Chi-Kuang Sun, Tzu-Ming Liu, Hung-Pin Chen
  • Patent number: 8501416
    Abstract: The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including meandering and wide channels. Microfluidic systems can provide an advantageous environment for performing various reactions and analysis due to a reduction in sample and reagent quantities that are required, a reduction in the size of the operating system, and a decrease in reaction time compared to conventional systems. Unfortunately, the small size of microfluidic channels can sometimes result in difficulty in detecting a species without magnifying optics (such as a microscope or a photomultiplier). A series of tightly packed microchannels, i.e., a meandering region, or a wide channel having a dimension on the order of millimeters, can serve as a solution to this problem by creating a wide measurement area.
    Type: Grant
    Filed: April 19, 2006
    Date of Patent: August 6, 2013
    Assignee: President and Fellows of Harvard College
    Inventors: Vincent Linder, Samuel K. Sia, George M. Whitesides, Max Narovlyansky, Adam Siegel
  • Patent number: 8501399
    Abstract: A method of detection comprising a conjugate of a randomly and asymmetrically branched dendritic polymer.
    Type: Grant
    Filed: July 12, 2010
    Date of Patent: August 6, 2013
    Assignee: ANP Technologies, Inc.
    Inventors: Ray Yin, Dujie Qin, Jing Pan
  • Patent number: 8486689
    Abstract: The invention relates to a microelectronic sensor device for the examination of target particles (1) that are bound to binding sites (3) at the binding surface (12) of a carrier (11). In a preferred embodiment, an input light beam (L1) is transmitted into the carrier (11), where a frustrated total internal reflection (FTIR) takes place at the binding surface (12). The amount of light in a resulting output light beam (L2) is detected by a light detector (31) and provides information about the presence of target particles at the binding surface. Moreover, an actuation unit (50) induces movements of the bound target particles (1) by an interaction with a magnetic field (B) or an electric field, particularly with a given modulation frequency (COIn), such that by a demodulation of the detector signal (S) effects of the target particles can be distinguished from background.
    Type: Grant
    Filed: October 31, 2008
    Date of Patent: July 16, 2013
    Assignee: Koninklijke Philips N.V.
    Inventors: Dominique Maria Bruls, Johannes Joseph Hubertina Barbara Schleipen, Josephus Arnoldus Henricus Maria Kahlman, Menno Willem Jose Prins
  • Patent number: 8481301
    Abstract: Disclosed are a centrifugal micro-fluidic structure for measuring glycated hemoglobin, a centrifugal micro-fluidic device for measuring glycated hemoglobin, and a method for measuring glycated hemoglobin. According to the disclosure, immunosorbent assay and affinity measurements are simultaneously conducted using only a single device in order to detect hemoglobin variants or interfering substances and, therefore, the detected results are applied to analysis of measurement results so as to eliminate and/or compensate for, or calibrate errors in measurement of, glycated hemoglobin, thereby more accurately measuring the glycated hemoglobin.
    Type: Grant
    Filed: September 16, 2010
    Date of Patent: July 9, 2013
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: In Wook Kim, Na Hui Kim
  • Patent number: 8470524
    Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. In particular, the medical device reduces interference with an optical signal which is indicative of the presence of an analyte in a bodily sample.
    Type: Grant
    Filed: July 21, 2011
    Date of Patent: June 25, 2013
    Assignee: Theranos, Inc.
    Inventors: Ian Gibbons, Michael O'Connell