Patents Examined by Nathan A. Bowers
  • Patent number: 10472612
    Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.
    Type: Grant
    Filed: December 22, 2016
    Date of Patent: November 12, 2019
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. Ingber, Hyun Jung Kim
  • Patent number: 10466232
    Abstract: Disclosed herein are bioreactor systems and methods of utilizing said systems.
    Type: Grant
    Filed: January 13, 2017
    Date of Patent: November 5, 2019
    Assignee: KIYATEC INC.
    Inventors: Matthew R. Gevaert, David E. Orr
  • Patent number: 10465152
    Abstract: Systems and methods for fractionating whole stillage from an ethanol production facility are provided. Whole stillage undergoes a separation of its liquid portion (thin stillage) from the solid portion (fiber cake). In some embodiments, the solids and liquids in whole stillage may be separated utilizing a screening centrifuge. The fiber cake may be dried to generate a high fiber animal feed. The thin stillage may be provided to a three-phase separator for separation into an oil emulsion, an aqueous clarified stillage, and a protein paste. The protein paste may be dried to generate a high protein animal feed with greater than about 45% protein content. The clarified thin stillage is condensed to yield a syrup with greater than around 60% solids. The oil emulsion is subjected to a pH adjustment to liberate the oil from the emulsion, which is then separated.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: November 5, 2019
    Assignee: POET Research, Inc.
    Inventor: Jason Alan Bootsma
  • Patent number: 10465185
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: May 28, 2019
    Date of Patent: November 5, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Patent number: 10455850
    Abstract: A method for recycling protein waste and producing an edible product for animal consumption comprises grinding animal carcasses or parts thereof and thereafter acidifying the waste. The acidified waste may then be emulsified, followed by a heat shock and then fed to animals. Alternatively, a second emulsification may be employed prior to being used for animal consumption. The emulsified products may be provided to animals as a liquid, or dried to a paste prior to use.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: October 29, 2019
    Assignee: Naturally Recycled Proteins
    Inventors: Jonathan Scott Darling, Don Scott Darling, Aminul Haque
  • Patent number: 10450543
    Abstract: Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. The connector is located at the distal end of the body and further includes at least one connecting post, a connecting part in mechanical communication with the connector, and a conductor located at least partially on or within the connector, wherein the conductor surrounds at least a portion of the connecting post. The elongated part also has a void and is located at the distal end of the connector. The void of the body, connector, and elongated part are in fluid communication.
    Type: Grant
    Filed: November 21, 2016
    Date of Patent: October 22, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jun-Keun Chang, Keun-Chang Cho, Chan-Il Chung, Neon-Cheol Jung, Seung Gin Huh
  • Patent number: 10443074
    Abstract: The present disclosure provides methods and devices for rapid and efficient modification of a variety of cell types, including mammalian cells, plant cells, archaea, yeasts, and bacteria, by novel methods of introducing exogenous materials, e.g. nucleic acids.
    Type: Grant
    Filed: September 28, 2018
    Date of Patent: October 15, 2019
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier
  • Patent number: 10443032
    Abstract: An apparatus (8) for isolating corneal endothelial cells (34) (CECs) includes a base portion (10) having an interior recessed opening (14) with a bottom surface (16). A convex projection (18) is centrally located on the bottom surface (16) and is configured to receive an inverted cornea (32). A top portion (12) is configured to mate with the base portion (10). The top portion (12) includes a fluid chamber (24) with a lower surface (20). The lower surface (20) has an opening (22) therein in which the convex projection (18) projects when the top portion (12) is mated with the base portion (10).
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: October 15, 2019
    Assignee: The Government of the United States, as represented by the Secretary of the Army
    Inventors: Robert Haggins, Jr., Erik Eaton, Jr., Zachary Murray, Timothy Varney
  • Patent number: 10428300
    Abstract: Maintaining pressure inside a culturing vessel is difficult when minimizing the size of a liquid supply path that supplies liquid and gas to a cell culturing vessel that performs two-level culturing (co-culturing). This cell culturing device includes: culturing vessels; a flow path having at least first supply ports capable of supplying liquid or gas to the culturing vessels and first discharge ports that discharge the gas from the culturing vessels, said flow path discharging gas from the first discharge ports to the atmosphere; and a filter in the flow path. A trap bottle is provided between the first discharge ports and the filter upon the flow path to collect moisture from the discharged gas. Thus, a liquid phase and a gas phase are separated in the trap bottle; moisture is prevented from reaching the filter; filter clogging is prevented; and the internal pressure of the culturing vessels can be maintained.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: October 1, 2019
    Assignee: HITACHI, LTD.
    Inventors: Masaharu Kiyama, Guangbin Zhou, Takayuki Nozaki
  • Patent number: 10421988
    Abstract: The method includes binding living cells to magnetic particles, adding them to a sensor array, uniformly distributing over the sensor array, magnetically fixing the magnetic particles having the bound cells over the sensor array, and adding substances to maintain and/or improve the cell vitality to the sensory array, and/or adding substances to worsen the cell vitality to the sensor array. The assembly includes a sensor array composed of sensors, which are designed to be in direct fluidic contact with a fluid, and a device for generating a magnetic field over the sensor array. A layer that comprises magnetic particles and living cells is formed on the sensor array.
    Type: Grant
    Filed: September 29, 2010
    Date of Patent: September 24, 2019
    Assignee: SIEMENS AKTIENGESELLSCHAFT
    Inventors: Walter Gumbrecht, Markus Klöpzig, Peter Paulicka, Harald Schmidt, Manfred Stanzel
  • Patent number: 10421937
    Abstract: A bio-reactive container and filter assembly to improve flow rates and mass transfer without increased pressure. An inlet filter membrane secured to the container defines a container chamber in combination with the container. An inlet filter membrane element may also be secured to the container to provide structural support to the membrane. An outlet membrane secured to the container defines a container chamber in combination with the container. An outlet filter membrane element may also be secured to the container to provide structural support to the outlet membrane. Inlet and/or outlet filters and/or filter elements are constructed to significantly increase surface area available for fluid and/or gas ingress and/or egress. Single and double layer membranes with mixtures of hydrophobic and hydrophilic characteristics are used to maximize perfusion and gasification/degasification of bio-reactive containers.
    Type: Grant
    Filed: March 4, 2016
    Date of Patent: September 24, 2019
    Assignee: Saint-Gobain Performance Plastics Corporation
    Inventors: ZhenWu Lin, Kenneth James Renfrew, Jacob Andrews
  • Patent number: 10421959
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: September 24, 2019
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Patent number: 10415058
    Abstract: In an illustrative embodiment, automated instruments comprising one or more flow-through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.
    Type: Grant
    Filed: September 30, 2018
    Date of Patent: September 17, 2019
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Don Masquelier, Phillip Belgrader
  • Patent number: 10412958
    Abstract: Provided is a device for vitrification-based cryopreservation which enables easy and reliable vitrification freezing of cells or tissues. The device of the present invention for cell or tissue cryopreservation by vitrification has a porous structure made of a material having a refractive index of 1.45 or less as a vitrification solution absorber.
    Type: Grant
    Filed: October 17, 2014
    Date of Patent: September 17, 2019
    Assignees: SCHOOL JURIDICAL PERSON KITASATO INSTITUTE, MITSUBISHI PAPER MILLS LIMITED
    Inventors: Kenji Momozawa, Atsushi Matsuzawa, Katsumitsu Susaki
  • Patent number: 10415009
    Abstract: A cell culture device comprises a cell culture membrane and a substrate fixed to the cell culture membrane. The cell culture membrane is made from a thermosetting resin and has a plurality of pores that are open to at least one surface including a surface on a side that is in contact with the substrate. The substrate is made from a thermoplastic resin. In a contact region between the cell culture membrane and the substrate, part of the substrate is extended to form bulges in the pores of the cell culture membrane that are open in the contact region. This configuration suppresses reduction of the strength of the cell culture membrane and reduction of the flatness of the cell culture membrane (membrane accuracy) in the cell culture device in which the cell culture membrane and the substrate are fixed to each other.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: September 17, 2019
    Assignees: TOYODA GOSEI CO., LTD., NATIONAL UNIVERSITY CORPORATION YAMAGATA UNIV.
    Inventors: Seitaro Taki, Hiroyuki Nakagawa, Atsuki Yoshimura, Masaru Tanaka
  • Patent number: 10408821
    Abstract: Disclosed herein are devices that include a top chamber including at least one port, a bottom chamber including at least one inlet and at least one outlet, wherein the opening of the at least one inlet is smaller than the opening of the at least one outlet, and a membrane located between the top chamber and the bottom chamber, wherein the membrane is fluidly coupled with the top chamber and the bottom chamber. Also disclosed herein are systems including the disclosed devices. The systems include liquid in one or more of the chambers of the device. Methods of using the devices and systems include producing a vacuum by flowing a liquid through the bottom chamber of the system. Due to the difference in size of the inlet and outlet in the bottom chamber, a vacuum is produced in the top chamber.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: September 10, 2019
    Assignee: Triad National Security, LLC
    Inventors: Pulak Nath, Jen-Huang Huang
  • Patent number: 10406527
    Abstract: The present invention provides a thermocycler comprising: a rotatable platform having a plurality of reaction wells or being adapted to receive a plurality of reaction containers, wherein the rotatable platform and/or the reaction wells are formed, at least in part, of a material which is adapted to be inductively heated by exposure to electromagnetic energy. An electromagnetic energy source is provided and is configured to direct electromagnetic energy at the rotatable platform, wherein the electromagnetic energy source surrounds a sufficient amount of the rotatable platform in order to heat the entire platform substantially simultaneously. In preferred embodiments, the electromagnetic energy source completely surrounds the rotatable platform. The invention further comprises a method of cycling a reaction mixture between predetermined temperatures utilising the novel thermocycler apparatus of the invention.
    Type: Grant
    Filed: October 15, 2014
    Date of Patent: September 10, 2019
    Assignee: BIO MOLECULAR SYSTEMS PTY LTD
    Inventors: John Corbett, Jason Austin
  • Patent number: 10399057
    Abstract: With this analysis device, air that has been heated by a heater is blown by a fan in the direction of an analysis receptacle rotary driver and an analysis receptacle that is rotationally driven by the analysis receptacle rotary driver. The analysis receptacle rotary driver rotates the analysis receptacle within the analysis chamber in the same direction as the direction of air flow produced by the fan.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: September 3, 2019
    Assignee: PHC HOLDINGS CORPORATION
    Inventors: Seiichiro Iketani, Takashi Ochi, Ryuji Shimizu, Masahiro Kouge, Shinichi Nakasuka, Kazuyoshi Mori
  • Patent number: 10393635
    Abstract: A device for isolating a microbe or a virion includes a semiconductor substrate; and a trench formed in the semiconductor substrate and extending from a surface of the semiconductor substrate to a region within the semiconductor substrate; wherein the trench has dimensions such that the microbe or the virion is trapped within the trench.
    Type: Grant
    Filed: April 29, 2016
    Date of Patent: August 27, 2019
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Yann Astier, David Esteban, Judson R. Holt, Henry K. Utomo
  • Patent number: 10391496
    Abstract: Methods, devices, systems, and kits useful for the collection and analysis of samples obtained by swabs are disclosed. Swab containers configured for receiving a swab containing a sample; cartridges for holding one or more of: a swab container, a swab, assay units, pipette tips, vessels, transport units, and implements; systems (which may include a sample processing device); kits; and methods for their use are disclosed. A swab container may include an entry port; an assay chamber having an assay port; a conduit comprising an interior channel connecting the entry port; and an interior channel providing fluidic communication between the entry port and assay chamber. An interior channel may be configured to squeeze a portion of a swab placed in or through the conduit. A cartridge may include a cartridge frame configured to receive one or more of swab containers, assay units, transport units, pipette tips, vessels or implements.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: August 27, 2019
    Assignee: Theranos IP Company, LLC
    Inventors: Clarissa Lui, Elizabeth A. Holmes