Patents Examined by Neil P Hammell
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Patent number: 11795472Abstract: The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.Type: GrantFiled: May 24, 2019Date of Patent: October 24, 2023Assignee: The Regents of the University of CaliforniaInventors: Jennifer A. Doudna, Jillian Banfield, David Burstein, Lucas Benjamin Harrington, Steven C. Strutt
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Patent number: 11781146Abstract: The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.Type: GrantFiled: May 13, 2019Date of Patent: October 10, 2023Assignees: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, FUSO PHARMACEUTICAL INDUSTRIES, LTD.Inventors: Yasuhiko Suzuki, Miki Nakagawa, Yayoi Kameda, Satoru Konnai, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Yamato Sajiki, Kazuhiko Ohashi, Shiro Murata, Yuzuru Kitahara, Keiichi Yamamoto
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Patent number: 11718838Abstract: The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based adenine base editors.Type: GrantFiled: July 21, 2021Date of Patent: August 8, 2023Assignee: Pairwise Plants Services. Inc.Inventors: Sharon Leigh Guffy, Joseph Matthew Watts
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Patent number: 11708568Abstract: The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based cytosine base editors.Type: GrantFiled: June 22, 2021Date of Patent: July 25, 2023Assignee: Pairwise Plants Services, Inc.Inventors: Sharon Leigh Guffy, Joseph Matthew Watts
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Patent number: 11649444Abstract: The present disclosure provides a Cas12i protein (e.g., non-naturally occurring, engineered) comprising an amino acid sequence having at least about 80% (e.g., at least about any of 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, or 100%) identity to the amino acid sequence as set forth in any one of SEQ ID NOs: 1-10 (preferably, SEQ ID NOs: 1-3 and 6, and more preferably, SEQ ID NO: 1).Type: GrantFiled: August 15, 2022Date of Patent: May 16, 2023Assignee: HuidaGene Therapeutics Co., Ltd.Inventors: Hainan Zhang, Xiangfeng Kong, Qijia Chen
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Patent number: 11639498Abstract: This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a DNA polymerase; and (b) a heterologous sequence-specific DNA binding domain. A method for copying a DNA template, as well as a kit for performing the same, are also described.Type: GrantFiled: August 20, 2018Date of Patent: May 2, 2023Assignee: New England Biolabs, Inc.Inventors: Pei-Chung Hsieh, Luo Sun, Thomas C. Evans, Jr., Theodore B. Davis, Andrew F. Gardner
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Patent number: 11634730Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.Type: GrantFiled: April 16, 2019Date of Patent: April 25, 2023Assignees: The Regents of the University of California, University of ViennaInventors: Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier
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Patent number: 11618861Abstract: This disclosure describes processes for using a single cellulosic feedstock or a combination of two or more different cellulosic feedstocks with a starch component to produce a fermented product. The process includes separating the components of the cellulosic feedstocks with fractionation, pretreating a component with wet fractionation with chemicals, hydrolysis and fermentation of the pretreated feedstock(s) to produce cellulosic biofuel. The process may include combining the cellulosic feedstock(s) with other components to a cook and/or a fermentation process, distilling and dehydrating the combined components to produce the bio fuel. The process may also include producing a whole stillage stream from the feedstock(s) and mechanically processing the whole stillage stream to produce a high-value protein animal feed.Type: GrantFiled: March 14, 2014Date of Patent: April 4, 2023Assignee: ICM, Inc.Inventors: Jeremy Edward Javers, Christopher Riley William Gerken, Charles C. Gallop, Samuel Vander Griend, Jesse Spooner, Jonathon Phillip Licklider, Laercio Malburg, Ryan Edward Hoefling, Douglas Bernard Rivers, Kurt A. Dieker
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Patent number: 11613743Abstract: The present invention claims a novel process for the production and purification of microbial collagenase (Microbial Collagenase EC 3.4.24.3) produced by the non-pathogenic aerobic bacterium Vibrio alginolyticus chemovar. iophagus (NCIMB Number: 11038, synonym LMG 3418, hereinafter called Vibrio alginolyticus), which said process provides high production levels of collagenase with a stable, reproducible, cheap fermentation process. The collagenase produced from Vibrio alginolyticus according to the process described herein also presents a specific activity superior to that of other microbial collagenases, is stable in aqueous solution, and can be frozen without significant damage.Type: GrantFiled: July 30, 2020Date of Patent: March 28, 2023Assignee: FIDIA FARMACEUTICI S.p.A.Inventors: Susanna Vaccaro, Michele Caputo, Christian Cuppari, Giovanni Gennari
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Patent number: 11591582Abstract: The present disclosure provides improved genome editing compositions and methods for editing a TCR? gene.Type: GrantFiled: October 11, 2017Date of Patent: February 28, 2023Assignee: 2seventy bio, Inc.Inventors: Jordan Jarjour, Mark Pogson
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Patent number: 11592453Abstract: Disclosed herein is a method of providing a metabololipidomic profile and SPM signature on the progress of the innate host defense response following blood clotting. The method can include the step of taking one or more measurements in a patient's blood sample, wherein the sample is obtained during the time-course of clotting or coagulation or following clotting or coagulation, of pro-thrombotic and pro-inflammatory mediators (eicosanoids) and specialized pro-resolving mediators SPMs. From these measurements, a personalized metabololipidomic profile can be obtained. By comparing the measurement to that taken from normal or reference blood, a comparison profile can be developed. The profile comparison profile can then be used to make a medical or therapeutic decision.Type: GrantFiled: November 22, 2017Date of Patent: February 28, 2023Assignee: The Brigham and Women's Hospital, Inc.Inventors: Charles N. Serhan, Nan Chiang, Paul Norris
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Patent number: 11555208Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.Type: GrantFiled: February 7, 2018Date of Patent: January 17, 2023Assignees: BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA, TOKAI UNIERSITY EDUCATIONAL SYSTEMInventors: Channabasavaiah B. Gurumurthy, Hiromi Miura, Masato Ohtsuka
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Patent number: 11549127Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.Type: GrantFiled: March 24, 2022Date of Patent: January 10, 2023Assignees: The Regents of the University of California, University of Vienna, Emmanuelle CharpentierInventors: Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier
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Patent number: 11547301Abstract: The present disclosure relates to methods of collecting and testing bacteria containing samples from within the gastrointestinal (GI) tract of a subject. The methods may include disposing an ingestible device in the GI tract, collecting a bacteria-containing sample from the GI tract, selectively lysing eukaryotic cells in the sample by combining the sample with a dried reagent, exposing bacteria in the sample to resazurin in the ingestible device to produce resorufin, emitting light from the ingestible device, the emitted light being filtered through an optical filter to control for scatter so that the light interacts with the resorufin to produce fluorescence, and measuring a total fluorescence from the resorufin; or a rate of change of fluorescence from the resorufin as a function of time within the GI tract of the subject; and correlating the measured parameter to a number of viable bacterial cells in the sample.Type: GrantFiled: March 10, 2021Date of Patent: January 10, 2023Assignee: Biora Therapeutics, Inc.Inventors: Sharat Singh, Mitchell Lawrence Jones, Christopher Loren Wahl
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Patent number: 11541084Abstract: A composition having Lactobacillus Plantarum strain GMNL-662 for promoting bone regrowth is provided. The Lactobacillus Plantarum strain GMNL-662 has an ability to promote the expression of osteogenic genes, inhibit the expression of osteoclast related genes, and promote the expression of osteogenesis-related cytokine TGF-?, so that the bone loss is improved.Type: GrantFiled: August 3, 2020Date of Patent: January 3, 2023Assignee: .GENMONT BIOTECH INCInventors: Yi-Hsing Chen, Wan-Hua Tsai, Chia-Hsuan Chou, Chi Chien Lin
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Patent number: 11541100Abstract: The present invention relates to a nanoperforator having a lipid-bilayer nanodisc and a membrane-structured protein surrounding the nanodisc and to a pharmaceutical composition having the nanoperforator as an active ingredient for preventing or treating viral infectious diseases. The use of the lipid-bilayer nanoperforator provided in the present invention can lead to the safe prevention or treatment of a disease caused by viral infection regardless of whether the virus is a variant or not, and thus the present invention can be widely used for the safe and effective treatment of viral infectious diseases.Type: GrantFiled: July 14, 2017Date of Patent: January 3, 2023Assignee: MVRIX CO., LTD.Inventors: Dae-Hyuk Kweon, Byoungjae Kong, Woo-Jae Chung, Baik Lin Seong, Sukchan Lee
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Patent number: 11529383Abstract: A method for modulating cellular activity in a subject, consisting essentially of the steps: (i) forming a liquid composition, (ii) subjecting the liquid composition to sequential harmonic oscillation to provide a liquid biochemical scaffold and, (iii) delivering a therapeutically effective amount of said liquid biochemical scaffold to said subject.Type: GrantFiled: April 29, 2022Date of Patent: December 20, 2022Assignee: Next2Me, LLCInventors: Ralph L Peterson, Renee D Williman
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Patent number: 11512286Abstract: The invention relates to methods for generating multipotent mammary stem cells from isolated and cultured human breast luminal cells. The method comprises the steps: 1. isolating and growing normal differentiated cells in vitro; 2. treating the differentiated cells with either a conditioned medium from active fibroblasts or cytokine. The invention also relates to multipotent mammary stem cells, cultures of the multipotent stem cells, differentiated cells, tissues, organs derived from the culture multipotent stem cells isolated by the methods disclosed and therapeutic and other uses for those cells thereof.Type: GrantFiled: July 9, 2018Date of Patent: November 29, 2022Assignee: KING FAISAL SPECIALIST HOSPITAL & RESEARCH CENTREInventors: Abdelilah Aboussekhra, Huda H. Al-Khalaf, Hazem Ghebeh
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Patent number: 11479826Abstract: Provided are detection means and method specific for the genus Cronobacter (E. sakazakii) which are sensitive by using a target molecule which is multiple existent in Cronobacter cells.Type: GrantFiled: November 20, 2014Date of Patent: October 25, 2022Assignee: Biotecon Diagnostics GmbHInventors: Cordt Grönewald, Kornelia Berghof-Jäger
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Patent number: 11479794Abstract: The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.Type: GrantFiled: March 24, 2022Date of Patent: October 25, 2022Assignees: The Regents of the University of California, University of ViennaInventors: Jennifer A. Doudna, Martin Jinek, Krzysztof Chylinski, Emmanuelle Charpentier