Patents Examined by Peter P. Tung
  • Patent number: 6423527
    Abstract: Compositions, methods and kits for diagnosing and treating cancer are provided. Therapeutic compositions may comprise agents that modulate the expression or activity of a springiness-1-phosphate lease (SPL). Such compositions may be administered to a mammal afflicted with cancer. Diagnostic methods and kits may employ an agent suitable for detecting alterations in endogenous SPL. Such methods and kits may be used to detect the presence of a cancer or to evaluate the prognosis of a known disease. SPL polypeptides, polynucleotides and antibodies are also provided.
    Type: Grant
    Filed: September 29, 1997
    Date of Patent: July 23, 2002
    Assignee: Children's Hospital Medical Center of Northern California
    Inventors: Julie D. Saba, Jianhui Zhou
  • Patent number: 6420177
    Abstract: The present invention relates to a method of improving the strain used for the production of erythromycin through the disruption of the melA gene.
    Type: Grant
    Filed: September 15, 1998
    Date of Patent: July 16, 2002
    Assignee: FermaLogic Inc.
    Inventors: J. Mark Weber, Minh B. Luu
  • Patent number: 6403349
    Abstract: The subject invention relates to the identification of a gene involved in the elongation of polyunsaturated fatty acids (i.e., “elongase”) and to uses thereof. In particular, elongase is utilized in the conversion of gamma linolenic acid (GLA) to dihomogamma linolenic acid (DGLA) and in the conversion of 20:4n-3 to eicosapentaenoic acid (EPA). DGLA may be utilized in the production of polyunsaturated fatty acids, such as arachidonic acid (AA) which may be added to pharmaceutical compositions, nutritional compositions, animal feeds, as well as other products such as cosmetics.
    Type: Grant
    Filed: September 2, 1998
    Date of Patent: June 11, 2002
    Assignee: Abbott Laboratories
    Inventors: Pradip Mukerji, Amanda Eun-Yeong Leonard, Yung-Sheng Huang, Jennifer Thurmond, Stephen J. Kirchner
  • Patent number: 6395530
    Abstract: Lipoprotein Lipase like polypeptides, nucleic acids encoding said polypeptides, antisense sequences, and antibodies to said polypeptides are disclosed. Also disclosed are methods for the preparation of said polypeptides in a recombinant system and for the use of said polypeptides to screen for agonists and or antagonists of said polypeptides. Also disclosed are methods and compositions for the treatment of disorders of lipid metabolism.
    Type: Grant
    Filed: December 5, 1997
    Date of Patent: May 28, 2002
    Assignee: Aventis Pharmaceuticals Inc.
    Inventors: Michael C. Jaye, Kim-Anh Thi Doan, John A. Krawiec, Kevin J. Lynch, Dilip V. Amin, Victoria J. South
  • Patent number: 6392024
    Abstract: A novel class of thermal hysteresis (antifreeze) proteins (THP) that have up to 100 times the specific activity of fish antifreeze proteins has been isolated and purified from the mealworm beetle, Tenebrio molitor. Internal sequencing of the proteins, leading to cDNA cloning and production of the protein in bacteria has confirmed the identity and activity of the 8.4 to 10.7 kDa THP. They are novel Thr- and Cys-rich proteins composed largely of 12-amino-acid repeats of cys-thr-xaa-ser-xaa-xaa-cys-xaa-xaa-ala-xaa-thr. At a concentration of 55 &mgr;g/mL, the THP depressed the freezing point 1.6° C. below the melting point, and at a concentration of ˜1 mg/mL the THP or its variants can account for the 5.5° C. of thermal hysteresis found in Tenebrio larvae. The THP function by an adsorption-inhibition mechanism and produce oval-shaped ice crystals with curved prism faces.
    Type: Grant
    Filed: June 26, 1997
    Date of Patent: May 21, 2002
    Assignee: Queen's University at Kingston
    Inventors: Laurie A. Graham, Yih-Cherng Liou, Virginia K. Walker, Peter L. Davies
  • Patent number: 6387667
    Abstract: A process for producing cytidine diphosphate choline is provided. The process comprises carrying out an enzymatic reaction using microorganisms having the enzyme activities of cytidine-5′-triphosphate synthetase (pyrG), cholinephosphate cytidylyltransferase (CCT) and choline kinase (CKI) and a microorganism capable of producing uridine-5′-triphosphate from orotic acid as the enzyme sources, and orotic acid and choline and/or phosphorylcholine as the substrates; allowing cytidine diphosphate choline to accumulate in the reaction mixture; and recovering cytidine diphosphate choline from said reaction mixture.
    Type: Grant
    Filed: January 28, 1993
    Date of Patent: May 14, 2002
    Assignee: Kyowa Hakko Kogyo, Co., Ltd.
    Inventors: Akihiko Maruyama, Tatsuro Fujio, Sadao Teshiba
  • Patent number: 6383769
    Abstract: Disclosed are a polypeptide having a &bgr;-fructofuranosidase activity obtainable by the expression of a microbial gene, a DNA encoding the polypeptide, a transformant obtained by introducing the DNA into an appropriate host, a process for producing the polypeptide comprising culturing the transformant to produce the polypeptide and collecting the produced polypeptide from the culture, and a method for fructofuranosyl transfer comprising a step of reacting a fructofuranosyl donor with a fructofuranosyl acceptor in the presence of the polypeptide.
    Type: Grant
    Filed: May 24, 1999
    Date of Patent: May 7, 2002
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Keiji Tsusaki, Michio Kubota, Hiroto Chaen
  • Patent number: 6358722
    Abstract: The present invention relates to heat tolerant phytases and DNA sequences which code therefor. The phytases are useful in hydrolyzing phytate to inositol and inorganic phosphates. The phytases are valuable feed additives.
    Type: Grant
    Filed: November 5, 1996
    Date of Patent: March 19, 2002
    Assignee: Roche Vitamins, Inc.
    Inventors: Adolphus van Loon, David Mitchell
  • Patent number: 6350597
    Abstract: Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fungal RS in a transformed host cell.
    Type: Grant
    Filed: March 26, 1999
    Date of Patent: February 26, 2002
    Assignee: E. I. du Pont de Nemours & Company
    Inventors: Paul Veikko Viitanen, Douglas Brian Jordan, Karen Onley Bacot
  • Patent number: 6350598
    Abstract: The invention provides MurD polypeptides and DNA (RNA) encoding MurD polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing MurD polypeptides to screen for antibacterial compounds.
    Type: Grant
    Filed: April 13, 1999
    Date of Patent: February 26, 2002
    Assignee: SmithKline Beecham Corp.
    Inventors: Nicola Gail Wallis, Joanna Lynn Fueyo, Michael Arthur Lonetto
  • Patent number: 6350602
    Abstract: A nucleotide sequence encoding phytase has been isolated and cloned. The coding sequence has been inserted into an expression construct which in turn has been inserted into a vector capable of transforming a microbial expression host. The transformed microbial hosts may be used to economically produce phytase on an industrial scale. The phytase produced via the present invention may be used in a variety of processes requiring the conversion of phytate to inositol and inorganic phosphate.
    Type: Grant
    Filed: January 20, 1999
    Date of Patent: February 26, 2002
    Assignee: DSM N.V.
    Inventors: Robert F. M. Van Gorcom, Willem Van Hartingsveldt, Petrus Andreas Van Paridon, Annemarie Eveline Veenstra, Rudolf G. M. Luiten, Gerardus C. M. Selten
  • Patent number: 6346607
    Abstract: Novel polypeptides, designated Apaf-1, which are capable of modulating apoptosis are provided. Compositions including Apaf-1 chimeras, nucleic acid encoding Apaf-1, and antibodies to Apaf-1 are also provided.
    Type: Grant
    Filed: November 5, 1999
    Date of Patent: February 12, 2002
    Assignee: Board of Regents, The University of Texas System
    Inventor: Xiaodong Wang
  • Patent number: 6344350
    Abstract: A novel isolate of Bacillus isolated from white pine is described. The isolate is used to produce monoterpene derivatives. Also described is a pinene hydroxylase enzyme from the isolate. The enzyme is a general allylic oxidizer.
    Type: Grant
    Filed: October 26, 1999
    Date of Patent: February 5, 2002
    Assignee: Board of Trustees of Michigan State University
    Inventors: Patrick J. Oriel, Natarajan S. Savithiry, Weijie Fu
  • Patent number: 6331428
    Abstract: Hexulose phosphate synthase was purified from Mycobacterium gastri to determine an amino acid sequence of the enzyme. Oligonucleotide primers to be used for PCR were synthesized on the basis of obtained amino acid sequence information. A DNA fragment, which was amplified by performing PCR by using a template of genomic DNA prepared from Mycobacterium gastri, was used as a probe so that colony hybridization was carried out with respect to a library comprising fragments obtained by digesting Mycobacterium gastri genomic DNA with PstI to obtain a positive clone. Thus, the DNA fragment which contains ORF encoding hexulose phosphate synthase was obtained. Furthermore, the DNA fragment cloned contained other ORF(ORF-1). The expression system of ORF-1 was constructed (pT-HPIS-1), and it was introduced into Escherichia coli cell. After the induction of the expression of ORF-1, the hexulose phosphate isomerase activity was found in the extract of cells harvoring pT-HPIS-1.
    Type: Grant
    Filed: November 4, 1998
    Date of Patent: December 18, 2001
    Assignee: Ajinomoto Co., Inc.
    Inventor: Nobuo Kato
  • Patent number: 6329191
    Abstract: A recombinant DNA encoding coffee bean &agr;-galactosidase permits the production of purified forms of this protein. The protein is useful in converting human Type B red blood cells into cells physiologically similar to Type O red blood cells. The availability of this enzyme permits more effective conversion than use of &agr;-galactosidase from other sources.
    Type: Grant
    Filed: August 30, 1993
    Date of Patent: December 11, 2001
    Assignee: Hawaii Biotechnology Group, Inc.
    Inventors: John M. Ivy, David E. Clements
  • Patent number: 6323013
    Abstract: Through function complementation of E. coli auxotrophs, the ultimate and pentultimate enzymes of the spinach riboflavin biosynthetic pathway have been cloned, namely, lumazine synthase (LS) and riboflavin synthase (RS). This invention relates to the isolation of nucleic acid fragments from plants or fungi that encode LS protein. The invention also relates to the isolation of nucleic acid fragments from plants or fungi that encode RS protein. In addition, the invention also relates to the construction of chimeric genes encoding all of a portion of LS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant LS in a transformed host cell. Furthermore, the invention also relates to the construction of chimeric genes encoding all of a portion of RS, in sense or antisense orientation, wherein the expression of the chimeric gene results in production of altered levels of plant or fungal RS in a transformed host cell.
    Type: Grant
    Filed: March 26, 1999
    Date of Patent: November 27, 2001
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Paul Veikko Viitanen, Douglas Brian Jordan, Onley Bacot Karen
  • Patent number: 6323017
    Abstract: The present invention relates to an isolated human protein having platelet activating factor acetylhydrolase activity.
    Type: Grant
    Filed: November 20, 1998
    Date of Patent: November 27, 2001
    Assignee: Suntory Limited
    Inventors: Hideki Adachi, Masafumi Tsujimoto, Hiroyuki Arai, Keizo Inoue
  • Patent number: 6315996
    Abstract: A method has been discovered for using lysostaphin as an effective antibiotic for topical treatment of Staphylococcus corneal infections (keratitis). Lysostaphin applied topically to the cornea by eye drops killed bacteria within the cornea; lysostaphin reduced the number of bacteria from approximately 10,000,000 viable bacteria colony forming units (“CFU”) in the untreated eye to essentially no viable bacteria in the treated eyes. Treatment by lysostaphin was more potent than any of the smaller antibiotics that have been previously tested (e.g., tetracyclines, erythromycins, cephalosporins, vancomycin, aminoglycosides, or fluoroquinolones). Moreover, topical application of lysostaphin was effective against the highly antibiotic-resistant Staphylococcus strains.
    Type: Grant
    Filed: April 9, 1999
    Date of Patent: November 13, 2001
    Assignee: Board of Supervisors of Louisiana State University and Agricultural and Mechanical College
    Inventor: Richard J. O'Callaghan
  • Patent number: 6307019
    Abstract: This invention provides an isolated nucleic acid which encodes Axin. This invention further provides an isolated nucleic acid which encodes a polypeptide comprising the amino acid sequence of Axin. This invention further provides a purified wildtype or mutant Axin. This invention further provides an oligonucleotide capable of distinguishing nucleic acids encoding mutant or wildtype Axin. This invention also provides various methods of use: such as a method for determining whether a subject carries a mutation in the axin gene, a method of determining whether a subject has a predisposition for cancer, a method for treating a subject who has a predisposition to cancer, a method for determining whether a subject has cancer, a method for detecting a mutation in cancerous cells of the subject, a method of suppressing cells unable to regulate themselves and a method for identifying a chemical compound which is capable of suppressing cells unable to regulate themselves.
    Type: Grant
    Filed: July 10, 1997
    Date of Patent: October 23, 2001
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Franklin Constantini, Li Zeng
  • Patent number: 6306634
    Abstract: An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I; wherein R1 is C1-C10 alkyl, C2-C10 alkenyl, C2-C10 alkynyl or C1-C4 haloalkyl, to produce the cyclopentenolone of formula I in (S)-form, and hybridizing to the base sequence of SEQ ID NO:1, is useful for the industrially favorable production of optically active cyclopentenolones of formula I which serve as the intermediates of drugs, agricultural chemicals or other active products.
    Type: Grant
    Filed: November 26, 1997
    Date of Patent: October 23, 2001
    Assignee: Sumitomo Chemical Company, Limited
    Inventors: Takeshi Ishii, Satoshi Mitsuda