Patents Examined by Peter P. Tung
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Nucleotide sequences that encode phosphatidylinositol-3' kinase associated proteins and uses thereof
Patent number: 6133419Abstract: Identification, characterization and expression of nucleotides that encode phosphatidylinositol-3' kinase associated protein(s) that bind to the intermediate SH2 domain on the regulatory subunit of PI3K, p85, by the associated protein(s) C-terminal amino acids, and that exhibit a bromodomain are described, as well as methods of using such proteins for medical applications, including diagnosis and treatment cell growth disorders.Type: GrantFiled: October 1, 1997Date of Patent: October 17, 2000Assignee: Onyx Pharmaceuticals, Inc.Inventor: Sylvia Braselmann -
Patent number: 6127144Abstract: An aminopeptidase inhibitor is used when expressing heterologous protein in a bacterial host, such as Streptomyces. Use of such an inhibitor inhibits degradation of the heterologous protein by aminopeptidases. Inhibitors are designed based upon the mechanism and substrate specificity of the target protease and expressed protein.Type: GrantFiled: October 16, 1997Date of Patent: October 3, 2000Assignee: Cangene CorporationInventors: Daniel Bartfeld, Michael J. Butler, Dany Hadary, David L. Jenish, Timothy J. Krieger, Lawrence T. Malek, Gisela Soostmeyer, Eva Walczyk, Phyllis Krygsman, Sheila Garven
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Patent number: 6127142Abstract: A method of producing a milk clotting enzyme including the steps of (a) fermenting a strain of Rhizomucor miehei or Aspergillus oryzae to form a fermentation product having a glycoslated Rhizomucor miehei aspartic protease and other proteins, and (b) subjecting a quantity of the fermentation product to a deglycosylating treatment to form a coagulant preparation having an at least partly deglycoslated aspartic protease and the other proteins. The at least partly deglycosylated protease has a milk clotting activity that is at least 10% higher than a milk clotting activity of the glycosylated aspartic protease.Type: GrantFiled: August 14, 1997Date of Patent: October 3, 2000Assignee: Chr. Hansen A/SInventors: Marianne Kirsten Harboe, Pia Bach Kristensen
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Patent number: 6114503Abstract: The present invention relates to a novel member of the blue-light photoreceptor family of receptors. In particular, isolated nucleic acid molecules are provided encoding the human hCRY2 receptor. hCRY2 polypeptides are also provided as are vectors, host cells, antibodies, and recombinant methods for producing the same.Type: GrantFiled: November 4, 1997Date of Patent: September 5, 2000Assignees: Human Genome Sciences, Inc., University of North Carolina at Chapel HillInventors: Ying-Fei Wei, Steven M. Ruben, Aziz Sancar, Shiao-Wen D. Hsu, Aleksey G. Kazantsev
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Patent number: 6110719Abstract: The invention provides a purified phytate enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in animal feed.Type: GrantFiled: March 1, 1999Date of Patent: August 29, 2000Assignee: Diversa CorporationInventor: Keith Kretz
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Patent number: 6110725Abstract: A recombinant strain of L. johnsonii which has capacities of surviving passage through the intestine, adhering to human intestinal cells and increasing phagocytosis of macrophages and which has a gene sequence code for D-lactate dehydrogenase which is a modified L. johnsonii gene sequence code and which produces only L(+)-lactate.Type: GrantFiled: May 1, 1998Date of Patent: August 29, 2000Assignee: Nestec S.A.Inventors: Michele Delley, Jacques Edouard Germond, Luciane Lapierre, Beat Mollet, Raymond David Pridmore
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Patent number: 6090911Abstract: The invention is based on the discovery that a block copolymer that includes .alpha.-helical blocks, e.g., terminal blocks, which form intermolecular coiled-coil structures, and one or more random-coil blocks, which link the .alpha.-helical blocks, can form suspensions that can reversibly gel to form monodisperse hydrogels. The transition between the gel and liquid phases depends on pH, temperature, concentration, and chemical structure. The copolymers can be synthesized biologically through genetic engineering.Type: GrantFiled: October 22, 1997Date of Patent: July 18, 2000Assignee: University of MassachusettsInventors: Wendy A. Petka, David A. Tirrell, Kevin P. McGrath
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Patent number: 6083715Abstract: Disclosed are methods and compositions for producing heterologous disulfide bond containing polypeptides in bacterial cells. In preferred embodiments the methods involve co-expression of a prokaryotic disulfide isomerase, such as DsbC or DsbG and a gene encoding a recombinant eukaryotic polypeptide. Exemplary polypeptides disclosed include tissue plasminogen activator.Type: GrantFiled: June 9, 1997Date of Patent: July 4, 2000Assignees: Board of Regents, The University of Texas System, Genentech, Inc.Inventors: George Georgiou, Ji Oiu, Paul Bessette, James Swartz
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Patent number: 6066488Abstract: The present invention provides nucleotide and amino acid sequences that identify and encode a new cathepsin C homolog (RCP) expressed in THP-1 cells. The present invention also provides for antisense molecules to the nucleotide sequences which encode RCP, expression vectors for the production of purified RCP, antibodies capable of binding specifically to RCP, hybridization probes or oligonucleotides for the detection of RCP-encoding nucleotide sequences, genetically engineered host cells for the expression of RCP, diagnostic tests for activation of monocyte/macrophages based on RCP-encoding nucleic acid molecules, and use of the protein to produce antibodies capable of binding specifically to the protein and use of the protein to screen for inhibitors.Type: GrantFiled: June 9, 1997Date of Patent: May 23, 2000Assignee: Incyte Pharmaceuticals, Inc.Inventors: Roger Coleman, Scott Michael Braxton, Jeffrey J. Seilhamer
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Patent number: 6060298Abstract: The present invention relates to an isolated polypeptide exhibiting phytase activity, the corresponding cloned DNA sequences, a process for preparing the polypeptide, and the use thereof for a number of industrial applications, in particular in animal feed. The novel phytase is derived from Peniophora lycii and has some interesting features, such as high initial affinity for the 6-position of phytic acid, a high intial rate of liberating phosphate from phytic acid and an exceptionally high specific activity.Type: GrantFiled: December 12, 1997Date of Patent: May 9, 2000Assignee: Novo Nordisk A/SInventors: S.o slashed.ren Flensted Lassen, Lisbeth Bech, Claus Crone Fuglsang, Anders Ohmann, Jens Breinholt, Peter Rahbet stergaard
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Patent number: 6054306Abstract: The present invention relates to an isolated polypeptide exhibiting phytase activity, the corresponding cloned DNA sequences, a process for preparing the polypeptide, and the use thereof for a number of industrial applications, in particular in animal feed. The novel phytase is derived from Peniophora lycii and has some interesting features, such as high initial affinity for the 6-position of phytic acid, a high intial rate of liberating phosphate from phytic acid and an exceptionally high specific activity.Type: GrantFiled: December 23, 1998Date of Patent: April 25, 2000Assignee: Novo Nordisk A/SInventors: S.o slashed.ren Flensted Lassen, Lisbeth Bech, Claus Crone Fuglsang, Anders Ohmann, Jens Breinholt, Peter Rahbet stergaard
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Patent number: 6043076Abstract: The enzyme 2,3-dihydroxybenzoic acid decarboxylase has industrial applications for the decarboxylation of ring mounted carboxyls, specifically the decarboxylation of 2,3-dihydroxybenzoic acid to catechol. This invention relates to the isolation of a nucleic acid sequence from Aspergillus niger that encodes an enzyme that decarboxylates 2,3-dihydroxybenzoic acid. This invention further discloses the nucleic acid sequence, the protein sequence, vectors comprising the nucleic acid sequence, cells transformed with the nucleic acid sequence, and methods for the production of 2,3-dihydroxybenzoic acid decarboxylase.Type: GrantFiled: August 18, 1998Date of Patent: March 28, 2000Assignee: Board of Regents of University of NebraskaInventor: Santha Ramakrishnan
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Patent number: 6033889Abstract: Gene sequences of superoxide dismutase of Aquifex pyrophilus which is one of hyperthermophile microorganism and protein expressed therefrom are provided, wherein the protein is used as a necessary medicament in treatment of inflammation, disease of autoimmunization, chromosome lesion and the like, and particularly, A. pyrophilus is a hyperthermophile which can grow at the optimum temperature of 85.degree. C., and thus the superoxide dismutase of A. pyrophilus has a higher thermal stability than other organisms, resulting in further broad applications in the pharmaceutical field.Type: GrantFiled: January 16, 1998Date of Patent: March 7, 2000Assignee: Korea Institute of Science and TechnologyInventors: Ye Sun Han, Yeon Gyu Yu, Sung Hou Kim, Jae Hwan Lim, Jae Ryeon Ryu, In Geol Choi
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Patent number: 6030602Abstract: The invention relates to conjugates of lipids and basic, membrane disturbing peptides, particularly compounds of the formula(R--CONH).sub.n --R.sup.3 (I)and(R--S--S).sub.n --R.sup.3 (II)wherein R is the hydrocarbyl moiety of a straight-chain or branched-chain, saturated or unsaturated aliphatic carboxylic acid, or a phospholipid moiety having a free valence bond; R.sup.3 is a basic membrane disturbing peptide having a free valence bond at one or two carbon atom(s); and n is 1 or 2. These conjugates can be used as a vector for transfecting a cell with a polynucleotide or any other anionic macromolecule.Type: GrantFiled: January 14, 1997Date of Patent: February 29, 2000Assignee: Hoffmann-La Roche Inc.Inventors: Jean-Yves Legendre, Andreas Supersaxo, Arnold Trzeciak
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Patent number: 6030824Abstract: The invention provides a human N-acetylneuraminate lyase (HNANL) and polynucleotides which identify and encode HNANL. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for treating or preventing disorders associated with expression of HNANL.Type: GrantFiled: February 1, 1999Date of Patent: February 29, 2000Assignee: Incyte PharmaceuticalsInventors: Jennifer L. Hillman, Purvi Shah, Neil C. Corley
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Patent number: 6030825Abstract: The invention provides a human cyclophilin-type peptidyl-prolyl cis/trans isomerase (CPCI) and polynucleotides which identify and encode CPCI. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of CPCI.Type: GrantFiled: August 19, 1998Date of Patent: February 29, 2000Assignee: Incyte Pharmaceuticals, Inc.Inventors: Jennifer L. Hillman, Neil C. Corley, Karl J. Guegler, Chandra Patterson
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Patent number: 6020173Abstract: A microorganism resistant to a threonine analogue, which has a plasmid containing part or whole of a biotin operon; and a process for producing biotin, which comprises culturing a microorganism described above in a medium to produce and accumulate biotin in the medium, and collecting biotin.Type: GrantFiled: February 19, 1997Date of Patent: February 1, 2000Assignees: Takeda Chemical Industries, Ltd., Shiseido Company, LimitedInventors: Naoyuki Kanzaki, Tomohiro Kawamoto, Junji Matsui, Kazuo Nakahama, Ohji Ifuku
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Patent number: 6013496Abstract: A method and test kit for determining milk production in bovines is described. The acetyl coenzyme A synthetase (ACS) production is determined and related to milk production. The method is useful in selection and/or breeding to enhance milk production. Recombinant microganisms, plasmids and ACS is also described.Type: GrantFiled: August 26, 1997Date of Patent: January 11, 2000Assignee: Board of Trustees operating Michigan State UniversityInventors: Robert M. Cook, Ahmed M. Raafat
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Patent number: 6008031Abstract: A diagnostic method for detecting a base pair mismatch in a DNA duplex, comprising the steps of contacting at least one strand of a first DNA molecule with the complementary strand of a second DNA molecule under conditions such that base pairing occurs contacting a DNA duplex potentially containing a base pair mismatch with a mispair recognition protein under conditions suitable for the protein to form a specific complex only with the DNA duplex having a base pair mismatch, and not with a DNA duplex lacking a base pair mismatch, and detecting any complex as a measure of the presence of a base pair mismatch in the DNA duplex.Type: GrantFiled: June 17, 1997Date of Patent: December 28, 1999Assignee: Duke UniversityInventors: Paul L. Modrich, Shin-San Su, Karin G. Au, Robert S. Lahue, Deani Lee Cooper, Leroy Worth, Jr.
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Patent number: 6001596Abstract: The invention provides a human growth-associated protease inhibitor heavy chain precursor (GAPIP) and polynucleotides which identify and encode GAPIP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating or preventing disorders associated with expression of GAPIP.Type: GrantFiled: May 7, 1998Date of Patent: December 14, 1999Assignee: Incyte Pharmaceuticals, Inc.Inventors: Jennifer L. Hillman, Karl J. Guegler, Chandra Patterson