Patents Examined by Rebecca E. Prouty
  • Patent number: 11214818
    Abstract: The present application relates to methods of producing one or more fatty alcohols and/or one or more fatty aldehydes from one or more unsaturated lipid moieties by combining the obtainment or production of the one or more unsaturated lipid moieties from a biological source with conversion by non-biological means of the one or more unsaturated lipid moieties to one or more fatty alcohols and/or one or more fatty aldehydes. The present application also relates to recombinant microorganisms having a biosynthesis pathway for the production of one or more unsaturated lipid moieties. The one or more fatty alcohols can further be chemically converted to one or more corresponding fatty acetates. The one or more fatty alcohols, one or more fatty aldehydes and/or one or more fatty acetates produced by the methods described herein may be one or more insect pheromones, one or more fragrances, one or more flavoring agents, or one or more polymer intermediates.
    Type: Grant
    Filed: June 6, 2017
    Date of Patent: January 4, 2022
    Assignee: Provivi, Inc.
    Inventors: Konrad B. Otte, Micah Sheppard, Vu Bui, Keith M. Wampler, Effendi Leonard
  • Patent number: 11178894
    Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: November 23, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Viktor Marius Boer, Priscilla Zwartjens, Eric Van Den Berg
  • Patent number: 11162121
    Abstract: The present invention relates to a method for producing a medium chain diamine and, more specifically, to a method for producing a medium chain diamine from an alcohol or alkane derived from a fatty acid, by culturing a recombinant microorganism from which a fatty aldehyde dehydrogenase gene in a ?-oxidative metabolic pathway and a ?-oxidative metabolic pathway related gene have been deleted, and also into which a ?-transaminase gene has been introduced. The recombinant microorganism disclosed in the present invention can prevent the additional oxidation and ?-oxidation metabolism of fatty aldehyde and can produce a medium chain diamine with a high yield by introducing an amine group to the terminus thereof.
    Type: Grant
    Filed: October 27, 2016
    Date of Patent: November 2, 2021
    Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Jung Oh Ahn, Hong Weon Lee, Gyu Yeon Park, Hee Suk Lee, Min Jeong Jang, Woo Young Jeon
  • Patent number: 11149258
    Abstract: The present invention relates to the field of poly- and oligosaccharides and their dietary effects. In particular it relates to a method of producing a branched ?-glucan. Further aspects of the invention are a branched ?-glucan comprising linear segments of (?1?4) linked D-glucose units interspersed with (?1?6) glucosidic linkages and having (?1?4,6) branching points; a food composition; and the use of an ?-glucanotransferase enzyme for reducing the digestible carbohydrates of a starch containing food material.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: October 19, 2021
    Assignee: Societe des Produits Nestle S.A.
    Inventors: Joana Gangoiti Munecas, Sander Sebastiaan Van Leeuwen, Tjaard Pijning, Lubbert Dijkhuizen, Christina Vafeiadi, Stephane Duboux
  • Patent number: 11104927
    Abstract: The present disclosure relates to a recombinant microorganism for producing crocin in which a gene (CCD2) encoding carotenoid cleavage dioxygenase, a gene (aldH) encoding crocetin dialdehyde dehydrogenase and a gene (UDP-glycosyltransftrase, UGT) encoding crocin biosynthesis enzyme are introduced, and a method for producing crocin using the same. Compared with the conventional method for producing crocin, which is produced in small amounts through a part of plants or callus, the production method using the recombinant microorganism of the present disclosure enables mass production of crocin.
    Type: Grant
    Filed: December 5, 2019
    Date of Patent: August 31, 2021
    Assignee: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Pyung Cheon Lee, Jun Ho Lee
  • Patent number: 11085029
    Abstract: The present disclosure relates to a novel acetohydroxy acid synthase, a microorganism comprising the same, or a method for producing an L-branched-chain amino acid using the same.
    Type: Grant
    Filed: October 21, 2020
    Date of Patent: August 10, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ae Ji Jeon, Byeong Cheol Song, Ji Hye Lee, Jong Hyun Kim, Hye Won Kim
  • Patent number: 11076609
    Abstract: Described are mutant Streptococcus thermophilus strains and mutant Lactobacillus delbrueckii subsp. bulgaricus strains that excrete glucose into milk when the milk is inoculated and fermented with such strains. Methods for obtaining such strains without genetic modification are described, including methods for obtaining such Streptococcus thermophilus strains by selecting and isolating from a pool of Streptococcus thermophilus strains derived from a galactose-fermenting Streptococcus thermophilus mother strain a pool of Streptococcus thermophilus strains which are resistant to 2-deoxyglucose, and selecting and isolating from the pool of Streptococcus thermophilus strains which are resistant to 2-deoxyglucose a Streptococcus thermophilus strain which exhibits a growth rate in M17 medium+2% galactose higher than its growth rate in M17 medium+2% glucose. The strains are useful for decreasing the lactose content of a fermented food product and for boosting growth of the probiotic BB-12®.
    Type: Grant
    Filed: October 17, 2019
    Date of Patent: August 3, 2021
    Assignee: CHR. HANSEN A/S
    Inventors: Eric Johansen, Kim Ib Soerensen, Mirjana Curic-Bawden, Mette Pia Junge
  • Patent number: 11078468
    Abstract: The present invention relates to proteins involved in fatty acid synthesis, such as fatty acid synthases (FAS) variants, comprising one or more polypeptide chains, wherein said polypeptide chain(s) comprise one or more subunits comprising a malonyl/palmitoyl transferase domain (MPT domain), acetyl transferase domain (AT domain), and ketoacyl synthase domain (KS domain), and at least one amino acid substitution in the MPT domain at a position corresponding to R130, in the AT domain at a position corresponding to I306, and/or in the KS domain, preferably in the acyl binding channel and/or at KS domain binding site to ACP, to modulate affinities of acyl intermediates, and optionally further amino acid substitution(s). The present invention relates to the respective polypeptide domains. The present invention further relates to nucleic acid molecules encoding the proteins (or the polypeptide domains) and to host cells containing said nucleic acid molecules.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: August 3, 2021
    Assignee: Johann Wolfgang Goethe-Universität Frankfurt am Main
    Inventors: Martin Grininger, Jan Gajewski, Eckhard Boles, Renata Pavlovic
  • Patent number: 11078502
    Abstract: A method and cell line for producing polyketides in yeast. The method applies, and the cell line includes, a yeast cell transformed with a polyketide synthase coding sequence. The polyketide synthase enzyme catalyzes synthesis of olivetol or methyl-olivetol, and may include Dictyostelium discoideum polyketide synthase (“DiPKS”). Wild type DiPKS produces methyl-olivetol only. DiPKS may be modified to produce olivetol only or a mixture of both olivetol and methyl-olivetol. The yeast cell may be modified to include a phosphopantethienyl transferase for increased activity of DiPKS. The yeast cell may be modified to mitigate mitochondrial acetaldehyde catabolism for increasing malonyl-CoA available for synthesizing olivetol or methyl-olivetol.
    Type: Grant
    Filed: February 19, 2018
    Date of Patent: August 3, 2021
    Assignee: Hyasynth Biologicals Inc.
    Inventors: Shoham Mookerjee, Alexander James Campbell, Zachary Douglas Wiltshire, Kevin John Chen
  • Patent number: 11060072
    Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: July 30, 2019
    Date of Patent: July 13, 2021
    Assignee: Codexis, Inc.
    Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
  • Patent number: 11044920
    Abstract: The dairy industry today faces a problem of providing an alternative to adding sweeteners to fermented milk products in order to achieve the desired sweet taste without the added calories. Furthermore, it would be highly advantageous to establish a method for reducing lactose in fermented milk products to a level which is acceptable for lactose-intolerant consumers. The above problems have been solved by providing mutant Streptococcus thermophilus strains and mutant Lactobacillus delbrueckii subsp. bulgaricus strains that excrete glucose to the milk when the milk is inoculated and fermented with such Streptococcus thermophilus strains and Lactobacillus delbrueckii subsp, bulgaricus strains.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: June 29, 2021
    Assignee: CHR. HANSEN A/S
    Inventors: Eric Johansen, Kim Ib Soerensen, Mirjana Curic-Bawden, Mette Pia Junge
  • Patent number: 11046980
    Abstract: The present invention provides production of hydroxylated fatty acid by a hydration reaction using a novel enzyme derived from Lactobacillus and using fatty acid as a substrate, and further, a production method of oxo fatty acid by an enzyme reaction or chemical oxidation reaction using the hydroxylated fatty acid as a substrate. In addition, a valuable novel rare fatty acid obtained by such production method is also provided.
    Type: Grant
    Filed: May 6, 2019
    Date of Patent: June 29, 2021
    Assignees: KYOTO UNIVERSITY, Noster, Inc.
    Inventors: Jun Ogawa, Shigenobu Kishino, Teruo Kawada, Nobuyuki Takahashi, Tsuyoshi Goto, Yasunori Yonejima
  • Patent number: 11046941
    Abstract: Provided herein are methods of using a Cas1 polypeptide to generate nucleic fragments from a DNA substrate. These methods may be performed in vitro or in vivo. Also provided are methods of screening for modulators of Cas1.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: June 29, 2021
    Assignee: The Regents of the University of California
    Inventors: Blake Wiedenheft, Kaihong Zhou, Jennifer A. Doudna
  • Patent number: 11041179
    Abstract: The disclosure herein relates to a method for preparing branched cyclodextrin and application thereof, and belongs to the technical field of synthesis of branched cyclodextrin. The method comprises the following steps: (1) dissolving maltodextrin in a phosphate buffer solution, and adding CGTase for reacting; (2) reducing enzyme activity by a physical method; and (3) adding a saccharifying enzyme to the reaction system of step (2), and performing high-temperature enzyme deactivation to obtain the branched cyclodextrin. The method has mild reaction conditions, and at the same time, cyclodextrin and unreacted substrates can be hydrolyzed via the weak coupling activity of CGTase, thereby realizing effective separation of the branched cyclodextrin.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: June 22, 2021
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Yuxiang Bai, Zhengyu Jin, Liuxi Xia, Yun Wu, Xueming Xu, Zhengjun Xie, Jinpeng Wang, Xing Zhou
  • Patent number: 11028416
    Abstract: The present disclosure describes the engineering of microbial cells for fermentative production of tyramine and provides novel engineered microbial cells and cultures, as well as related tyramine production methods.
    Type: Grant
    Filed: June 26, 2019
    Date of Patent: June 8, 2021
    Assignee: ZYMERGEN INC.
    Inventors: Michael Shareef Siddiqui, Stefan de Kok, Alexander Glennon Shearer, Franklin Lu, Cara Ann Tracewell, Steven M. Edgar
  • Patent number: 11028419
    Abstract: The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: June 8, 2021
    Assignee: Glycosyn LLC
    Inventors: Massimo Merighi, John M. McCoy, Matthew Ian Heidtman
  • Patent number: 11028380
    Abstract: The present invention provides a Cas9 platform to facilitate single-site nuclease gene editing precision within a human genome. For example, a Cas9 nuclease/DNA-targeting unit (Cas9-DTU) fusion protein precisely delivers a Cas9/sgRNA complex to a specific target site within the genome for subsequent sgRNA-dependent cleavage of an adjacent target sequence. Alternatively, attenuating Cas9 binding using mutations to the a protospacer adjacent motif (PAM) recognition domain makes Cas9 target site recognition dependent on the associated DTU, all while retaining Cas9's sgRNA-mediated DNA cleavage fidelity. Cas9-DTU fusion proteins have improved target site binding precision, greater nuclease activity, and a broader sequence targeting range than standard Cas9 systems. Existing Cas9 or sgRNA variants (e.g., truncated sgRNAs (tru-gRNAs), nickases and FokI fusions) are compatible with these improvements to further reduce off-target cleavage.
    Type: Grant
    Filed: December 19, 2018
    Date of Patent: June 8, 2021
    Assignee: University of Massachusetts
    Inventors: Scot Andrew Wolfe, Mehmet Fatih Bolukbasi, Ankit Gupta, Erik J Sontheimer, Nadia Amrani
  • Patent number: 11028414
    Abstract: An inositol preparation method by enzymatic catalysis uses starch and cellulose or substrates thereof as substrates. Raw materials are converted to inositol by in vitro multi-enzyme reaction system in one pot. The yield from the substrate to inositol is significantly improved by process optimization and adding new enzymes. The new enzymes can promote the phosphorolysis of starch or cellulose and utilization of glucose, which is the final production after the phosphorolysis of starch and cellulose. The inositol preparation method described herein has great potentials in industrial production of inositol because of high inositol yield, easy scale-up, low production cost, and lower impact to environment.
    Type: Grant
    Filed: March 4, 2019
    Date of Patent: June 8, 2021
    Assignee: BONUMOSE, INC.
    Inventors: Yi-Heng Percival Zhang, Chun You
  • Patent number: 11028421
    Abstract: The disclosure discloses recombinant Pseudomonas plecoglossicida for producing L-xylose and application thereof, and belongs to the technical field of bioengineering. According to the disclosure, a synthesized 2-ketogluconate reductase gene and a 2,5-diketogluconate reductase gene derived from Corynebaterium ATCC 31090 and a pyruvate decarboxylase gene derived from Saccharomyces cerevisiae are successfully expressed in a host P. plecoglossicida by a double plasmid system, and an obtained genetically engineered strain is fermented for 56 h in a shake flask, where the yield of L-xylose reaches 16.2 g/L, and the transformation rate reaches 20.3%; the obtained genetically engineered strain is fermented for 48 h and 44 h in 3 L and 15 L fermentors, respectively, where the yields of L-xylose reach 37.6 g/L and 45.8 g/L, respectively, and the glucose transformation rates are 47.0% and 57.3%, respectively.
    Type: Grant
    Filed: November 28, 2019
    Date of Patent: June 8, 2021
    Assignee: LINKCHEM CO. LTD.
    Inventor: Xi Lu
  • Patent number: 11021697
    Abstract: The present disclosure relates to a novel acetohydroxy acid synthase, a microorganism comprising the same, or a method for producing an L-branched-chain amino acid using the same.
    Type: Grant
    Filed: November 22, 2019
    Date of Patent: June 1, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ae Ji Jeon, Byeong Cheol Song, Ji Hye Lee, Jong Hyun Kim, Hye Won Kim