Patents Examined by Rebecca E. Prouty
  • Patent number: 10487346
    Abstract: The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: November 26, 2019
    Assignee: Glycosyn LLC
    Inventors: Massimo Merighi, John M. McCoy, Matthew Ian Heidtman
  • Patent number: 10472654
    Abstract: Disclosed are very high optically pure D- and L-lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L-lactic acids using the strains, wherein the deposit number of the D-lactic acid fermentation production strain is CGMCC No. 11059, and the deposit number of the L-lactic acid fermentation production strain is CGMCC No. 11060.
    Type: Grant
    Filed: November 20, 2017
    Date of Patent: November 12, 2019
    Assignee: Tianjin University of Science and Technology
    Inventors: Zhengxiang Wang, Kangming Tian, Dandan Niu, Fuping Lu
  • Patent number: 10450559
    Abstract: Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
    Type: Grant
    Filed: January 29, 2018
    Date of Patent: October 22, 2019
    Assignee: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Aine Quimby, Shuang-Yong Xu, Shengxi Guan, Hua Wei, Penghua Zhang, Dapeng Sun, Siu-hong Chan
  • Patent number: 10428361
    Abstract: The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.
    Type: Grant
    Filed: February 19, 2016
    Date of Patent: October 1, 2019
    Assignee: BASF SE
    Inventors: Vaidotas Navickas, Michael Breuer, Michael Puhl, Melanie Weingarten, Kai-Uwe Baldenius, Wolfgang Siegel
  • Patent number: 10407705
    Abstract: The present invention provides an isolated fungal cell that is capable of producing one or more biomass-degrading enzymes and that exhibits increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. Also provided is a fermentation processes for producing one or more biomass-degrading enzymes comprising a fungal cells exhibiting increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. The biomass-degrading enzymes produced by the isolate fungal cell and fermentation processes of the present invention may be used in a process to produce soluble sugars from biomass.
    Type: Grant
    Filed: October 19, 2017
    Date of Patent: September 10, 2019
    Assignee: IOGEN ENERGY CORPORATION
    Inventors: Philippe J. Dufresne, Adam H. Colville, Barbara Fryzuk, Loreta Gudynaite-Savitch, Christopher M. D. Hindle, Boguslaw Ploch, John J. Tomashek
  • Patent number: 10407668
    Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Grant
    Filed: October 13, 2017
    Date of Patent: September 10, 2019
    Assignee: Codexis, Inc.
    Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
  • Patent number: 10400224
    Abstract: This invention refers to the obtainment of a modified lipolytic enzyme that was isolated, expressed and purified from the heterologous expression. The gene sequence that codifies for the basal enzyme was obtained based on a thermo acidophilus organism of the acidobacteraceae family. This basal enzyme that comes from a thermo acidophilus organism, it is able to hydrolyze lipid substrates (triacylglycerols) united to middle chain fatty acids (C6-C10) such as tributyrine and tricapryln, among others. It also can carry out other inverse reactions to the hydrolysis such as synthesis reactions. On the other hand, this enzyme has enantioselective preference on (S) substrates of profens esters such as ibuprofen, naproxen and others. The enantioselective lipolytic basal enzyme was modified in its terminal C end to add an amino acid histidine tail that gives a higher efficiency in its purification process.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: September 3, 2019
    Assignees: CORPORACIÓN CORPOGEN, PONTIFICA UNIVERSIDAD JAVERIANA
    Inventors: María Mercedes Zambrano Eder, Sandra Baena Garzón, Gina Pilar López Ramírez
  • Patent number: 10392609
    Abstract: Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: August 27, 2019
    Assignees: Oxyrane UK Limited, VIB vzw, Universiteit Gent, Vrije Universiteit Brussel
    Inventors: Nico Luc Marc Callewaert, Wouter Vervecken, Petra Sophie Tiels, Han Karel Remaut, Kathleen Camilla Telesphore Alida Maria Piens
  • Patent number: 10370686
    Abstract: The present invention provides for a genetically modified yeast host cell capable of producing one or more fatty acids, or fatty acid-derived compounds, or a mixture thereof, comprising: (a) increased expression of acetyl-CoA carboxylase (such as ACC1), (b) increased expression of one or more fatty acid synthases (such as FAS1 and FAS2), and (c) optionally reduced expression of one or more enzymes involved in or in the ?-oxidation pathway (such as peroxisomal transporters PXA1 and PXA2, and ?-oxidation enzymes POX1, POX2, and POX3).
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: August 6, 2019
    Assignee: The Regents of the University of California
    Inventors: Weerawat Runguphan, Jay D. Keasling
  • Patent number: 10364449
    Abstract: The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: July 30, 2019
    Assignee: Glycom A/S
    Inventors: Gyula Dekany, Pauline Peltier-Pain, Dóra Molnár-Gábor, Markus Jondelius Hederos
  • Patent number: 10351833
    Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: July 16, 2019
    Assignee: Novozymes A/S
    Inventors: Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Gylstorff, Jesper Vind, Christian Jackel
  • Patent number: 10351888
    Abstract: The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: July 16, 2019
    Assignee: Jiangnan University
    Inventors: Wanmeng Mu, Bo Jiang, Shuhuai Yu, Yingying Zhu, Tao Zhang
  • Patent number: 10337033
    Abstract: Disclosed are methods for the synthesis of chiral alcohols by Sortase A-mediated oxidoreductase oligomers, which relates to the field of biocatalysis. In the present disclosure, oxidoreductase oligomers were used as biocatalysts for chiral alcohol preparation. Compared to wild-type enzymes, the oxidoreductase oligomers significantly improved catalytic activity and thermal stability. The sortase A-mediated oxidoreductase oligomers had 6-8 folds improvement in specific activity over that of the wild-type enzymes. The oligomers displayed a Tm value 6-12° C. higher than that of the wild-type, suggesting the sortase A-mediated oxidoreductase oligomers significantly improved thermostability of the enzymes. The oxidoreductase oligomers catalyzed asymmetric transformation to produce (S)-1-phenyl-1,2-ethanediol or (R)-1-phenethyl alcohol within 3-6 hr, with an optical purity of 98%-100% and a yield of 98%-99%.
    Type: Grant
    Filed: July 15, 2017
    Date of Patent: July 2, 2019
    Assignee: Jiangnan University
    Inventors: Rongzhen Zhang, Yan Xu, Kunpeng Li
  • Patent number: 10316087
    Abstract: Disclosed are a heterodimeric TCR containing artificial interchain disulfide bond between the variable region of ? chain and the constant region of ? chain, a preparing method therefor and a use thereof.
    Type: Grant
    Filed: March 29, 2016
    Date of Patent: June 11, 2019
    Assignee: GUANGDONG XIANGXUE LIFE SCIENCES, LTD.
    Inventor: Yi Li
  • Patent number: 10316304
    Abstract: The invention relates to chimeric endonucleases, comprising a endonuclease and a heterologous DNA binding domain comprising one or more Zn2C6 zinc fingers, as well as methods of targeted integration, targeted deletion or targeted mutation of polynucleotides using chimeric endonucleases.
    Type: Grant
    Filed: November 26, 2010
    Date of Patent: June 11, 2019
    Assignee: BASF PLANT SCIENCE COMPANY GMBH
    Inventors: Andrea Hlubek, Christian Biesgen, Hans Wolfgang Höffken
  • Patent number: 10308972
    Abstract: The subject of the present invention is a whole-cell catalysis process for converting substrates of cytochrome P450 monooxygenases of eukaryotic origin into valuable biotechnological products. The subject of the present invention is also microorganisms genetically engineered to achieve those biotransformations with high rates and processes to prepare these microorganism strains.
    Type: Grant
    Filed: June 17, 2014
    Date of Patent: June 4, 2019
    Assignee: SANOFI CHIMIE
    Inventors: Adrian Gerber, Frank Hannemann, Sabrina Bleif, Michael Kleser, Rita Bernhardt
  • Patent number: 10308724
    Abstract: The subject matter of the invention is dextrans which have between 95% and 99% of ?-1,6 glucosidic bonds, a weight-average molar mass Mw at least equal to 0.7×109 g.mol?1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.
    Type: Grant
    Filed: June 19, 2015
    Date of Patent: June 4, 2019
    Assignees: INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE BORDEAUX, INSTITUT POLYTECHNIQUE DE BORDEAUX
    Inventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Florent Grimaud, Pierre Monsan, Agnès Sabate, Catherine Garnier, Marguerite Dols-Lafargue, Patrick Lucas
  • Patent number: 10294464
    Abstract: Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
    Type: Grant
    Filed: November 28, 2017
    Date of Patent: May 21, 2019
    Assignee: New England Biolabs, Inc.
    Inventors: Zhenyu Zhu, Aine Quimby, Shengxi Guan, Dapeng Sun, Yishu Huang, Xuhui Lai, Siu-hong Chan, Xianghui Li, Shuang-Yong Xu, Chunhua Zhang
  • Patent number: 10294501
    Abstract: The present invention provides a production method of oxo fatty acid, as well as rare fatty acids such as conjugated fatty acid, hydroxylated fatty acid, partially saturated fatty acid and the like, which uses 4 kinds of enzymes (fatty acid-hydratase, hydroxylated fatty acid-dehydrogenase, oxo fatty acid-isomerase, oxo fatty acid-enone reductase) derived from Lactobacillus plantarum including lactic acid bacteria and the like. Furthermore, the present invention also provides a more efficient production method of oxo fatty acid and the like, which partly uses a chemical oxidation reaction in combination.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: May 21, 2019
    Assignees: KYOTO UNIVERSITY, NITTO PHARMACEUTICAL INDUSTRIES, LTD.
    Inventors: Jun Ogawa, Shigenobu Kishino, Sakayu Shimizu, Yasunori Yonejima
  • Patent number: 10287521
    Abstract: The present invention provides compositions and methods related to the production and use of enzymes suitable for reducing the amount of steryl glycosides or saturated monoacyl glycerols in a lipid mixture.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: May 14, 2019
    Inventors: Hugo Menzella, Salvador Peiru, Leandro Vetcher