Abstract: The present application relates to a composition for producing D-psicose comprising (a) a D-psicose 3-epimerase, a strain expressing the enzyme or a culture of the strain, and (b) at least one salt selected from the group consisting of an aluminate and an iodate, and a method for producing D-psicose from D-fructose or a method for increasing the conversion of D-fructose into D-psicose comprising adding (b) to (a).
Type:
Grant
Filed:
December 23, 2016
Date of Patent:
February 4, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Jung Eun Kim, Yang Hee Kim, Seong Bo Kim, Seung Won Park, Ji Hyun Kang
Abstract: A alcohol dehydrogenase of sequence ID no. 1 containing metal ions and a quinone cofactor, or in addition, a functional variant exhibiting a sequence identity of at least 80%, preferably at least 86%, especially preferred at least 89% and at least one redox cofactor for the transformation of at least one trichothecene exhibiting a hydroxyl group on the C-3 atom, as well as a method for the enzymatic transformation of trichothecenes and a trichothecene-transforming additive.
Type:
Grant
Filed:
March 27, 2015
Date of Patent:
January 7, 2020
Assignee:
ERBER AKTIENGESELLSCHAFT
Inventors:
Eva-Maria Binder, Barbara Weber, Claudia Bernard
Abstract: The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
Type:
Grant
Filed:
May 15, 2018
Date of Patent:
November 26, 2019
Assignee:
Glycosyn LLC
Inventors:
Massimo Merighi, John M. McCoy, Matthew Ian Heidtman
Abstract: Disclosed are very high optically pure D- and L-lactic acid fermentation production strains and construction methods thereof and the method for preparing very high optically pure D- and L-lactic acids using the strains, wherein the deposit number of the D-lactic acid fermentation production strain is CGMCC No. 11059, and the deposit number of the L-lactic acid fermentation production strain is CGMCC No. 11060.
Type:
Grant
Filed:
November 20, 2017
Date of Patent:
November 12, 2019
Assignee:
Tianjin University of Science and Technology
Inventors:
Zhengxiang Wang, Kangming Tian, Dandan Niu, Fuping Lu
Abstract: Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
Abstract: The present invention relates to biocatalytic methods, comprising purely enzymatic, mixed enzymatic-fermentative and purely fermentative methods, for the direct single-step conversion of L-fucitol to L-fucose, in order to easily obtain L-fucose at high amounts and levels of purity. Suitable recombinant microorganisms and fungi are further disclosed and also the use thereof in said method for the single-step conversion to L-fucose.
Type:
Grant
Filed:
February 19, 2016
Date of Patent:
October 1, 2019
Assignee:
BASF SE
Inventors:
Vaidotas Navickas, Michael Breuer, Michael Puhl, Melanie Weingarten, Kai-Uwe Baldenius, Wolfgang Siegel
Abstract: The present invention provides an isolated fungal cell that is capable of producing one or more biomass-degrading enzymes and that exhibits increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. Also provided is a fermentation processes for producing one or more biomass-degrading enzymes comprising a fungal cells exhibiting increased or decreased expression or copy number of a polynucleotide encoding a PtaB-like protein. The biomass-degrading enzymes produced by the isolate fungal cell and fermentation processes of the present invention may be used in a process to produce soluble sugars from biomass.
Type:
Grant
Filed:
October 19, 2017
Date of Patent:
September 10, 2019
Assignee:
IOGEN ENERGY CORPORATION
Inventors:
Philippe J. Dufresne, Adam H. Colville, Barbara Fryzuk, Loreta Gudynaite-Savitch, Christopher M. D. Hindle, Boguslaw Ploch, John J. Tomashek
Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
Type:
Grant
Filed:
October 13, 2017
Date of Patent:
September 10, 2019
Assignee:
Codexis, Inc.
Inventors:
Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
Abstract: This invention refers to the obtainment of a modified lipolytic enzyme that was isolated, expressed and purified from the heterologous expression. The gene sequence that codifies for the basal enzyme was obtained based on a thermo acidophilus organism of the acidobacteraceae family. This basal enzyme that comes from a thermo acidophilus organism, it is able to hydrolyze lipid substrates (triacylglycerols) united to middle chain fatty acids (C6-C10) such as tributyrine and tricapryln, among others. It also can carry out other inverse reactions to the hydrolysis such as synthesis reactions. On the other hand, this enzyme has enantioselective preference on (S) substrates of profens esters such as ibuprofen, naproxen and others. The enantioselective lipolytic basal enzyme was modified in its terminal C end to add an amino acid histidine tail that gives a higher efficiency in its purification process.
Type:
Grant
Filed:
April 30, 2015
Date of Patent:
September 3, 2019
Assignees:
CORPORACIÓN CORPOGEN, PONTIFICA UNIVERSIDAD JAVERIANA
Abstract: The present invention provides for a genetically modified yeast host cell capable of producing one or more fatty acids, or fatty acid-derived compounds, or a mixture thereof, comprising: (a) increased expression of acetyl-CoA carboxylase (such as ACC1), (b) increased expression of one or more fatty acid synthases (such as FAS1 and FAS2), and (c) optionally reduced expression of one or more enzymes involved in or in the ?-oxidation pathway (such as peroxisomal transporters PXA1 and PXA2, and ?-oxidation enzymes POX1, POX2, and POX3).
Type:
Grant
Filed:
January 25, 2016
Date of Patent:
August 6, 2019
Assignee:
The Regents of the University of California
Abstract: The application discloses a method for producing an oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, said method comprising a step of: culturing, in a culture medium containing a fucosylated, sialylated or N-acetyl-glucosaminylated lactose trisaccharide as acceptor, a genetically modified cell having a recombinant gene that encodes an enzyme capable of modifying said acceptor or one of the necessary intermediates in the biosynthetic pathway of the oligosaccharide of at least four monosaccharide units, advantageously an HMO, particularly an HMO of only four monosaccharide units, from said acceptor.
Type:
Grant
Filed:
September 5, 2014
Date of Patent:
July 30, 2019
Assignee:
Glycom A/S
Inventors:
Gyula Dekany, Pauline Peltier-Pain, Dóra Molnár-Gábor, Markus Jondelius Hederos
Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
Type:
Grant
Filed:
May 13, 2014
Date of Patent:
July 16, 2019
Assignee:
Novozymes A/S
Inventors:
Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Gylstorff, Jesper Vind, Christian Jackel
Abstract: The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects.
Type:
Grant
Filed:
April 15, 2016
Date of Patent:
July 16, 2019
Assignee:
Jiangnan University
Inventors:
Wanmeng Mu, Bo Jiang, Shuhuai Yu, Yingying Zhu, Tao Zhang
Abstract: Disclosed are methods for the synthesis of chiral alcohols by Sortase A-mediated oxidoreductase oligomers, which relates to the field of biocatalysis. In the present disclosure, oxidoreductase oligomers were used as biocatalysts for chiral alcohol preparation. Compared to wild-type enzymes, the oxidoreductase oligomers significantly improved catalytic activity and thermal stability. The sortase A-mediated oxidoreductase oligomers had 6-8 folds improvement in specific activity over that of the wild-type enzymes. The oligomers displayed a Tm value 6-12° C. higher than that of the wild-type, suggesting the sortase A-mediated oxidoreductase oligomers significantly improved thermostability of the enzymes. The oxidoreductase oligomers catalyzed asymmetric transformation to produce (S)-1-phenyl-1,2-ethanediol or (R)-1-phenethyl alcohol within 3-6 hr, with an optical purity of 98%-100% and a yield of 98%-99%.
Abstract: The invention relates to chimeric endonucleases, comprising a endonuclease and a heterologous DNA binding domain comprising one or more Zn2C6 zinc fingers, as well as methods of targeted integration, targeted deletion or targeted mutation of polynucleotides using chimeric endonucleases.
Type:
Grant
Filed:
November 26, 2010
Date of Patent:
June 11, 2019
Assignee:
BASF PLANT SCIENCE COMPANY GMBH
Inventors:
Andrea Hlubek, Christian Biesgen, Hans Wolfgang Höffken
Abstract: Disclosed are a heterodimeric TCR containing artificial interchain disulfide bond between the variable region of ? chain and the constant region of ? chain, a preparing method therefor and a use thereof.
Abstract: The subject matter of the invention is dextrans which have between 95% and 99% of ?-1,6 glucosidic bonds, a weight-average molar mass Mw at least equal to 0.7×109 g.mol?1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.
Type:
Grant
Filed:
June 19, 2015
Date of Patent:
June 4, 2019
Assignees:
INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE BORDEAUX, INSTITUT POLYTECHNIQUE DE BORDEAUX
Inventors:
Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Florent Grimaud, Pierre Monsan, Agnès Sabate, Catherine Garnier, Marguerite Dols-Lafargue, Patrick Lucas
Abstract: The subject of the present invention is a whole-cell catalysis process for converting substrates of cytochrome P450 monooxygenases of eukaryotic origin into valuable biotechnological products. The subject of the present invention is also microorganisms genetically engineered to achieve those biotransformations with high rates and processes to prepare these microorganism strains.
Type:
Grant
Filed:
June 17, 2014
Date of Patent:
June 4, 2019
Assignee:
SANOFI CHIMIE
Inventors:
Adrian Gerber, Frank Hannemann, Sabrina Bleif, Michael Kleser, Rita Bernhardt
Abstract: Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.