Patents Examined by Rebecca M Giere
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Patent number: 10775380Abstract: This invention concerns patentable devices configured to capture cancer stem cells and cell clusters. After capturing such cells and/or clusters, the cancerous cells are subjected to whole genome sequencing. The resulting genomic sequence information is then compared to that for “normal” or non-diseased tissue (obtained, for example, from either the same patient, or a population sample, etc.) in order to identify the specific genetic mutation(s) present in the CSCs. Further analysis then correlates the genetic mutations with cell growth signaling pathways typically found with tumor metastases. Armed with this information, an oncologist can then develop a specifically targeted therapy that utilizes approved drugs or drug candidates undergoing clinical testing to address the identified driver mutations and thus effect a “targeted” therapy tailored to the particular patient's disease.Type: GrantFiled: April 5, 2016Date of Patent: September 15, 2020Assignee: TUMORGEN, INC.Inventors: Jeffrey K. Allen, Alexander G. Allen, Austin E. Allen
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Patent number: 10775376Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: January 17, 2020Date of Patent: September 15, 2020Assignee: THE UNIVERSITY OF BRITISH COLUMBIAInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Patent number: 10774378Abstract: The invention relates to a new method of determining the presence, absence or one or more characteristics of multiple analytes. The invention concerns coupling a first analyte to a membrane containing a detector and investigating the first analyte using the detector. The invention also concerns coupling a second analyte to the membrane and investigating the second analyte. The first analyte is uncoupled from the membrane prior to investigating the second analyte. The invention also relates to polynucleotide sequencing.Type: GrantFiled: March 31, 2015Date of Patent: September 15, 2020Assignee: Oxford Nanopore Technologies Ltd.Inventors: James Anthony Clarke, Marion Louise Crawford, James White
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Patent number: 10775377Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: June 25, 2020Date of Patent: September 15, 2020Assignee: The University of British ColumbiaInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Patent number: 10775374Abstract: The invention presents a stationary phase for detecting at least one specific analyte in a mixture. The stationary phase includes a solid phase divided into a plurality of zones for transporting liquid by capillary force. The solid phase includes at least one first zone suitable for receiving a liquid sample, a second zone on which a receptor is immobilized, wherein the receptor is suitable for binding to a ligand, and a third zone on which quantum dots are immobilized, function as FRET donors and are linked to at least one suitable FRET acceptor via at least one oligonucleotide linker. The stationary phase exhibits improved detection sensitivity and allows simultaneous and multiparametric detection of a wide variety of analytes. The invention proposes uses of the stationary phase and also presents a method for detecting at least one specific analyte in a mixture.Type: GrantFiled: November 7, 2017Date of Patent: September 15, 2020Assignee: Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V.Inventors: Ralph Sperling, Ralf Himmelreich, Tobias Schunck, Raphael Thiermann
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Patent number: 10775378Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: June 25, 2020Date of Patent: September 15, 2020Assignee: The University of British ColumbiaInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Patent number: 10761030Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.Type: GrantFiled: September 9, 2014Date of Patent: September 1, 2020Assignee: Labrador Diagnostics LLCInventors: Elizabeth A. Holmes, Ian Gibbons, Timothy M. Kemp, John Howard, Shaunak Roy
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Patent number: 10753932Abstract: An immunoassay test apparatus, including: a label signal sensor that detects label signals emitted by at least one label conjugated to an antibody or antigen of an immunoassay test device such as a lateral flow test strip or microfluidic cartridge, and outputs sensor data representing the detected label signals; a label imaging component that processes the sensor data to generate label data representing spatial locations of the detected label signals; a test result image generator that processes the label data to generate test result image data representing a visual image of the spatial arrangement of the detected label signals; and a display component that displays the test result image to a user of the immunoassay test apparatus to enable the user to evaluate the result of the immunoassay test.Type: GrantFiled: September 4, 2015Date of Patent: August 25, 2020Assignee: Axxin Pty LtdInventor: William R. Hopper
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Patent number: 10753927Abstract: A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte.Type: GrantFiled: October 4, 2012Date of Patent: August 25, 2020Assignee: ALERE TECHNOLOGIES GMBHInventors: Thomas Kaiser, Klaus-Peter Mobius, Torsten Schulz, Thomas Uhlig, Alexander Von Schenk Zu Schweinsberg, Eugen Ermantraut, Jens Tuchscheerer
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Patent number: 10753933Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: March 11, 2020Date of Patent: August 25, 2020Assignee: The University of British ColumbiaInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Patent number: 10746737Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: March 12, 2020Date of Patent: August 18, 2020Assignee: The University of British ColumbiaInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Patent number: 10722534Abstract: A magnetic nanoaggregate-embedded bead (NAEB), a manufacturing method thereof and a bioparticle detection method using the same are disclosed. The magnetic NAEB has a protective nanoshell, noble metal nanoparticles, Raman reporter molecules and at least one magnetic nanoparticle. The protective nanoshell covers the noble metal nanoparticles, the Raman reporter molecules and the at least one magnetic nanoparticle. The noble metal nanoparticles, the Raman reporter molecules and the at least one magnetic nanoparticle form a magnetic nanoaggregate. Preferably, a chemical modification is performed on the magnetic NAEB, such that at least one targeting molecule is formed on an outer wall of the protective nanoshell, wherein a type of the targeting molecule is corresponding to a type of the bioparticle to be detected.Type: GrantFiled: July 12, 2017Date of Patent: July 28, 2020Assignee: National Chung Cheng UniversityInventors: Lai-Kwan Chau, Shih-Ying Yen
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Patent number: 10725033Abstract: Assays used in conjunction with a thermal contrast reader are disclosed. In the assay, the test strip includes materials that can develop a thermal response if a target analyte is present in a sample. Linear flow assays include nanoparticles with high affinity binding to the analyte. Binding of the nanoparticles with an analyte in the sample is detected using thermal contrast. Analytes over a broad range of concentrations are detected in the linear flow assays. Methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast reader are also disclosed.Type: GrantFiled: November 2, 2017Date of Patent: July 28, 2020Assignees: REGENTS OF THE UNIVERSITY OF MINNESOTA, THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTOInventors: John C. Bischof, Zhenpeng Qin, Warren Chan, Taner Akkin, Li Zhan
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Patent number: 10718768Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: March 12, 2020Date of Patent: July 21, 2020Assignee: The University of British ColumbiaInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Patent number: 10718762Abstract: Herein is reported a method for the determination of the simultaneous binding of a bispecific antibody to a first and a second antigen comprising the steps of a) incubating a cell expressing cell-membrane bound FRET-donor-tagged first antigen and FRET-acceptor-tagged second antigen with the bispecific antibody, and b) determining the simultaneous binding of the bispecific antibody by determining the energy transfer from the FRET-donor to the FRET-acceptor.Type: GrantFiled: March 30, 2018Date of Patent: July 21, 2020Assignee: Hoffmann-La Roche Inc.Inventors: Stefan Seeber, Jens Fischer, Georg Fertig
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Patent number: 10712282Abstract: This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.Type: GrantFiled: September 9, 2014Date of Patent: July 14, 2020Assignee: Labrador Diagnostics LLCInventors: Elizabeth A. Holmes, Ian Gibbons, Timothy M. Kemp, John Howard, Shaunak Roy
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Patent number: 10705085Abstract: Disclosed are compositions and methods for the use of lipoplex nanoparticle chips and arrays in the detection of/diagnosis of a disease or condition.Type: GrantFiled: September 29, 2016Date of Patent: July 7, 2020Assignee: Ohio State Innovation FoundationInventors: Ly James Lee, Kwang Joo Kwak, Bo Yu, Yun Wu, Andrew Lee
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Patent number: 10697962Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.Type: GrantFiled: March 11, 2020Date of Patent: June 30, 2020Assignee: The University of British ColumbiaInventors: Anupam Singhal, Carl L. G. Hansen, John W. Schrader, Charles A. Haynes, Daniel J. Da Costa
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Detection chip, detection kit, detection system, and method for detecting detection target substance
Patent number: 10677732Abstract: A detection chip includes: a liquid container; and a light-transmitting substrate in which one of two surfaces facing each other faces an inside of the liquid container and an LSPR structure that generates localized surface plasmon resonance by light irradiation is disposed on the other surface of the two surfaces or in a region sandwiched between the one surface and the other surface.Type: GrantFiled: June 25, 2018Date of Patent: June 9, 2020Assignee: KONICA MINOLTA, INC.Inventors: Chie Osawa, Akihiro Maezawa, Makoto Mochizuki