Abstract: A transposable element, or transposon, isolated from Bacillus thuringiensis (B.t.) and designated as transposon Tn5401. The invention also includes a method of using this transposon in a site-specific recombination system for construction of recombinant B.t. strains that contain insecticidal B.t. toxin protein genes and that are free of DNA not native to B.t.

Abstract: The present invention provides a gene expression regulatory DNA and a process for preparing a protein using the same.A DNA derived from the isocitrate lyase (ICL) gene of a coryneform bacterium regulates expression of a structural gene encoding a protein when incorporated into a vector DNA together with said structural gene and introduced into a host coryneform bacterium, and a useful protein can be efficiently produced using the DNA.

Abstract: The subject invention concerns novel peptides which have the property of interfering with the biosynthesis of the enzyme trypsin. This property enables the use of these peptides to, for example, inhibit the formation of progeny in blood-ingesting insects, since trypsin is an essential enzyme for food digestion which provides the essential building blocks for egg development in such insects.

Abstract: Disclosed herein are the DNA sequences encoding human and bovine acidic and basic fibroblast growth factors (FGF). Expression of these sequences results in practical amounts of proteins useful in effecting wound healing.

Abstract: A host vector system comprising Rhizopus species such as Rhizopus niveus (IFO4810) and a plasmid derived from Phycomycetes such as Mucor, Phycomyces and Absidia wherein the plasmid is contained in a chromosome and/or cytoplasm of the Rhizopus species. The host vector system is suitable for the extracellular production of proteins and enzymes. A process for preparing the host vector system is also provided.

Abstract: A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter; P2 promoter; P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.

Abstract: A method for producing in vivo stable single-stranded DNAs in eucaryotic cells. The DNAs are multicopy single-stranded DNA (msDNA) structures constituted by a RNA and a DNA portion. The group of genes (retrons) producing said coupled RNA and DNA portions of the msDNAs and the gene encoding reverse transcriptase (RT). The transformed eucaryotes harboring these retrons. The new msDNAs which are encoded by the new retrons. The msDNAs can be used as vectors for antisense DNA and for amplification of inserted genes.

Abstract: This invention relates to DNA encoding drug resistance to cis-platin. The invention also includes expression products of, and vectors and hosts comprising the DNA sequence encoding cis-platin resistance. Also included are immunodiagnostic assays of cis-platin resistance and assays for screening materials having a modulating effect on DNA encoding the cis-platin resistance gene and on the expression product thereof. The invention is further directed to antagonists to the cis-platin resistance gene and the expression product thereof. Recombinant and pharmaceutical means making use of the cis-platin resistance gene and its expression product are also provided.

Abstract: The present invention relates to the unexpected discovery that xanthan gum can be produced from filtered whey or milk products, including whey permeates and milk permeates, by a fermentation process which uses organisms which are capable of converting lactose to xanthan gum.

Abstract: A method for sequential cloning of chromosomal DNA of a target organism is disclosed. A first DNA segment homologous to the chromosomal DNA to be sequentially cloned is isolated. The first segment has a first restriction enzyme site on either side. A first vector product is formed by ligating the homologous segment into a suitably designed vector. The first vector product is circularly integrated into the target organism's chromosomal DNA. The resulting integrated chromosomal DNA segment includes the homologous DNA segment at either end of the integrated vector segment. The integrated chromosomal DNA is cleaved with a second restriction enzyme and ligated to form a vector-containing plasmid, which is replicated in a host organism. The replicated plasmid is then cleaved with the first restriction enzyme. Next, a DNA segment containing the vector and a segment of DNA homologous to a distal portion of the previously isolated DNA segment is isolated.

Abstract: Methods and recombinant vectors suitable for accomplishing the in vivo alteration of a nucleic acid molecule are disclosed. The invention in particular discloses the use of recombinases such as Cre to accomplish in vivo recombination.

Abstract: The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.

Abstract: A chimeric gene directing the synthesis of hybrid recombinant fusion protein in a suitable expression vector has been constructed. The fusion protein possesses the property of selective cytotoxicity against specific virus-infected cells. A CD4(178)-PE40 hybrid fusion protein has been made for selectively killing HIV-infected cells.

Abstract: A recombinant plasmid vector is provided which is capable of replicating and expressing in a Coryneform bacterial cell, which plasmid vector is pEC 701, pEC 702, pEC 801, pEC 830 or pEC 901.

Abstract: Disclosed are the plasmid pBUL1 having a restriction endonuclease cleavage map as shown in FIG. 1 and having a length of about 7.9 kbp and its derivatives.The plasmid was isolated from Lactobacillus delbrueckii subsp. bulgaricus M-878.The plasmid is useful as a vector for breeding various microorganisms such as lactic acid bacteria, and the derivatives thereof are useful also as a shuttle vector (lactic acid bacteria--Escherichia coli).

Abstract: A hybrid upstream regulatory sequence including an upstream regulatory sequence of E. coli origin and yeast upstream regulatory sequence, which hybrid upstream regulatory sequence can function in a yeast cell; a hybrid promoter including the regulatory sequence of E. coli origin and a TATA region of a yeast promoter, which hybrid promoter can also function in a yeast cell; a hybrid promoter including the upstream regulatory sequence of E. coli origin and a yeast promoter, which hybrid promoter can function in a yeast cell; and a plasmid useful for a test of an upstream regulatory sequence, including a TATA region of a yeast promoter, a structural gene containing a translation start codon, and a yeast origin of replication.

Abstract: A vector having a gene for resistance to an antibiotic otherwise capable of killing a host yeast cell, the gene being transcribed from a yeast promoter sequence and the vector being capable of being integrated into a chromosome of the host yeast cell; and a diploid or greater ploidy yeast cell transformed by such a vector with heterologous DNA.

Abstract: Minactivin (also known as Plasminogen Activator Inhibitor-2 [PAI-2]), a protein inactivator of urokinase-type plasminogen activator, has been shown to be a natural inactivator of this plasminogen activator which is associated with invasive tumors, and is therefore indicated as a crucial element in the body's normal defense against tumor invasion and metastasis. It may be produced by the cultivation of minactivin-producing cells in vitro, and recovery of the cell culture supernatant. By controlling the culture conditions, the protein minactivin may be produced in a partially purified form which may be used for diagnosis and treatment of tumors. The specification discloses purification of biologically active native minactivin, as well as peptides derived from minactivin and their amino acid sequences.

Abstract: A virus having the identifying characteristics of ATCC VR2396 is disclosed. This virus has the trait of enhanced polyhedra production stability and resists forming a few polyhedra mutant virus. A method of protecting crops from insects comprising applying an insecticidally effective amount of virus having the identifying characteristics of ATCC VR2396 is also disclosed.

Abstract: The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.