Patents Examined by Richard Hutson
  • Patent number: 9862938
    Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
    Type: Grant
    Filed: May 7, 2014
    Date of Patent: January 9, 2018
    Assignee: Life Technologies Corporation
    Inventors: Michael D. Smith, Robert Jason Potter, Gulshan Dhariwal, Gary F. Gerard, Kim Rosenthal, Jun E. Lee
  • Patent number: 9856463
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: January 2, 2018
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Patent number: 9840698
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: December 12, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Patent number: 9834761
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: February 9, 2016
    Date of Patent: December 5, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Patent number: 9803180
    Abstract: The present invention relates to isolated polypeptides that are derived from wildtype Bacillus subtilis S-Adenosylmethionine (SAM) synthase or from a biologically active fragment thereof, wherein said isolated polypeptides comprise an amino acid sequence that, in relation to the amino acid sequence of said wildtype Bacillus subtilis SAM synthase or of the biologically active fragment thereof, comprises at least one amino acid substitution, selected from the group consisting of amino acid substitutions at positions I317 and I105. The present invention further relates to respective isolated nucleic acids, vectors, host cells, uses and methods for the production of SAM derivatives.
    Type: Grant
    Filed: September 4, 2014
    Date of Patent: October 31, 2017
    Assignee: LEIBNIZ-INSTITUT FÜR PFLANZENBIOCHEMIE STIFTUNG DES ÖFFENTLICHEN RECHTS
    Inventors: Martin Dippe, Wolfgang Brandt, Ludger A. Wessjohann, Hannes Rost, Andrea Porzel
  • Patent number: 9796965
    Abstract: The present invention generally relates to detection of a target nucleic acid in standard PCR, real-time PCR, RT PCR, and real-time RT PCR. One aspect of the invention provides mutant DNA polymerase enzymes that are resistant to PCR inhibitors, such as dye, blood, and soil. Another aspect of the invention provides for methods of real-time PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing dye, blood, and/or soil. Another aspect of the invention provides for methods of standard PCR assays using mutant DNA polymerase enzymes resistant to PCR inhibitors with samples containing blood and/or soil.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: October 24, 2017
    Assignee: DNA Polymerase Technology, Inc.
    Inventors: Milko Kermekchiev, Lyubka Kirilova, Zhian Zhang
  • Patent number: 9771565
    Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
    Type: Grant
    Filed: August 23, 2010
    Date of Patent: September 26, 2017
    Assignee: Life Technologies Corporation
    Inventors: Jun Lee, Robert Jason Potter
  • Patent number: 9771608
    Abstract: The present invention relates to an isolated DNA molecule encoding a fagopyritol synthase. A method for producing a fagopyritol, an insulin mediator, an insulin mediator analog, an insulin mediator homolog, or an insulin mediator inhibitor is also described. The method includes providing a fagopyritol synthase, providing a substrate comprising a galactosyl donor and a galactosyl acceptor, and combining the fagopyritol synthase with the substrate under conditions effective produce a fagopyritol, an insulin mediator, an insulin mediator analog, an insulin mediator homolog, or an insulin mediator inhibitor.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: September 26, 2017
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Ralph L. Obendorf, Takashi Ueda
  • Patent number: 9765315
    Abstract: The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a ?-glucosidase, a a-glucosidase, a xylanase, a mannanse, a ?-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a ?-galactanase, an unsaturated ?-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts.
    Type: Grant
    Filed: August 15, 2013
    Date of Patent: September 19, 2017
    Assignee: Bangladesh Jute Research Institute
    Inventors: Maqsudul Alam, Mohammed S. Islam, Mohammed M. Hossen, Mohammed S. Haque, Mohammed M. Alam
  • Patent number: 9765312
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: September 19, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Patent number: 9765311
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: January 15, 2016
    Date of Patent: September 19, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Patent number: 9764008
    Abstract: A method of treating an inflammatory disease is disclosed. The method comprises administering to the subject a therapeutically effective amount of a polypeptide comprising a pro-domain of TNF-alpha converting enzyme (TACE), said polypeptide being devoid of a catalytic domain of said TACE, said polypeptide comprising a modification at a site selected from the group consisting of R58, R56 and K57 which renders said polypeptide resistant to furin degradation said polypeptide being capable of downregulating an activity of TACE, thereby treating the inflammatory disease.
    Type: Grant
    Filed: May 9, 2013
    Date of Patent: September 19, 2017
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Irit Sagi, Eitan Wong, Ran Afik
  • Patent number: 9765377
    Abstract: The present invention relates to an isolated DNA molecule encoding a fagopyritol synthase. A method for producing a fagopyritol, an insulin mediator, an insulin mediator analogue, an insulin mediator homologue, or an insulin mediator inhibitor is also described. The method includes providing a fagopyritol synthase, providing a substrate comprising a galactosyl donor and a galactosyl acceptor, and combining the fagopyritol synthase with the substrate under conditions effective produce a fagopyritol, an insulin mediator, an insulin mediator analogue, an insulin mediator homologue, or an insulin mediator inhibitor.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: September 19, 2017
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Ralph L. Obendorf, Takashi Ueda
  • Patent number: 9758804
    Abstract: The present invention relates to an isolated DNA molecule encoding a fagopyritol synthase. A method for producing a fagopyritol, an insulin mediator, an insulin mediator analogue, an insulin mediator homologue, or an insulin mediator inhibitor is also described. The method includes providing a fagopyritol synthase, providing a substrate comprising a galactosyl donor and a galactosyl acceptor, and combining the fagopyritol synthase with the substrate under conditions effective produce a fagopyritol, an insulin mediator, an insulin mediator analogue, an insulin mediator homologue, or an insulin mediator inhibitor.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: September 12, 2017
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Ralph L. Obendorf, Takashi Ueda
  • Patent number: 9758771
    Abstract: Provided are a beta prime subunit mutant of RNA polymerase, a microorganism of the Corynebacterium genus including a polynucleotide coding the same, and a method for producing L-lysine by culturing the same.
    Type: Grant
    Filed: June 24, 2014
    Date of Patent: September 12, 2017
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Sang Hee Park, Jun Ok Moon, Kwang Ho Lee
  • Patent number: 9752171
    Abstract: The present invention provides recombinant nucleic acid constructs comprising a xylose isomerase polynucleotide, a recombinant fungal host cell comprising a recombinant xylose isomerase polynucleotide, and related methods.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: September 5, 2017
    Assignee: Codexis, Inc.
    Inventors: Ezhilkani Subbian, Xiyun Zhang, Benjamin N. Mijts, Catherine M. Cho
  • Patent number: 9745557
    Abstract: The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22.
    Type: Grant
    Filed: May 15, 2015
    Date of Patent: August 29, 2017
    Assignee: BAYER PHARMA AKTIENGESELLSCHAFT
    Inventors: Stefan Golz, Bernd Kalthof, Svetlana Markova, Ludmila Frank, Eugene Vysotski
  • Patent number: 9738876
    Abstract: Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: January 20, 2015
    Date of Patent: August 22, 2017
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Keith A. Bauer, Ellen Fiss, David Harrow Gelfand, Edward S. Smith, Shawn Suko, Thomas W. Myers
  • Patent number: 9732364
    Abstract: The present invention relates to use of inducible promoters in the production of methionine by fermentation. The present invention concerns a method for the production of methionine, its precursors or derivatives in a fermentative process comprising the following steps: culturing a modified microorganism in an appropriate culture medium comprising a source of carbon, a source of sulphur and a source of nitrogen, and recovering methionine and/or its derivatives from the culture medium, wherein in said modified microorganism, the expression of at least one gene involved in methionine production is under the control, direct or indirect, of a heterologous inducible promoter. The invention also concerned the modified microorganism used in the method.
    Type: Grant
    Filed: December 13, 2010
    Date of Patent: August 15, 2017
    Assignee: EVONIK DEGUSSA GMBH
    Inventors: Rainer Figge, Perrine Vasseur
  • Patent number: 9719073
    Abstract: Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: August 1, 2017
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Robin Emig, Insil Park, Lei Jia, Molly He, Jeremiah Hanes, Harold Lee, Fred Christians, Satwik Kamtekar, Erik Miller