Patents Examined by Richard Hutson
  • DNA polymerases with increased 3?-mismatch discrimination
    Patent number: 9284542
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: April 28, 2014
    Date of Patent: March 15, 2016
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Polymerases
    Patent number: 9273352
    Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: March 1, 2016
    Assignee: Illumina Cambridge Limited
    Inventors: Geoffrey Paul Smith, Roberto Rigatti, Tobias William Barr Ost, Shankar Balasubramanian, Raquel Maria Sanches-Kuiper
  • Method for controlling the biological activity of a protein in a vertebrate cell
    Patent number: 9273115
    Abstract: The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor ?, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site-specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
    Type: Grant
    Filed: January 22, 2010
    Date of Patent: March 1, 2016
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, UNIVERSITE DE STRASBOURG
    Inventors: Daniel Metzger, Pierre Chambon, Huimin Zhao, John Katzenellenbogen
  • DNA polymerases with increased 3?-mismatch discrimination
    Patent number: 9273293
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: April 21, 2014
    Date of Patent: March 1, 2016
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Mutant citrobacter freundii phytase polypeptide
    Patent number: 9273295
    Abstract: The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.
    Type: Grant
    Filed: September 14, 2011
    Date of Patent: March 1, 2016
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Andrei Miasnikov, Vijay Kumar, Oliver Kensch, Klaus Pellangahr, Birgitta Leuthner, Ulrich Kettling, Andre Koltermann
  • DNA polymerases with increased 3?-mismatch discrimination
    Patent number: 9267121
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: February 23, 2016
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • DNA polymerases with increased 3'-mismatch discrimination
    Patent number: 9267169
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: February 23, 2016
    Assignee: Roche MolecularSystems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • DNA polymerases with increased 3'-mismatch discrimination
    Patent number: 9267120
    Abstract: Disclosed are mutant DNA polymerases having increased 3?-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.
    Type: Grant
    Filed: March 28, 2014
    Date of Patent: February 23, 2016
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Fred Reichert, Keith Bauer, Thomas W. Myers
  • Polymerase
    Patent number: 9228179
    Abstract: An engineered DNA polymerase characterised in that the polymerase exhibits an enhanced ability to process nucleic acid in the presence of environmental and biological inhibitors compared to wild type DNA polymerase.
    Type: Grant
    Filed: February 18, 2013
    Date of Patent: January 5, 2016
    Assignee: MEDICAL RESEARCH COUNCIL
    Inventors: Philipp Holliger, Marc D'Abbadie, Claudia Baar
  • Polypeptides having nucleic acid binding activity
    Patent number: 9212352
    Abstract: Polynucleotides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided. Methods of promoting the annealing of complementary nucleic acid strands are provided. Methods of increasing the processivity of a DNA polymerase are provided. Methods of enhancing the activity of a nucleic acid modification enzyme are provided. Fusion proteins are provided. Methods of using fusion proteins are provided. Kits are provided.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: December 15, 2015
    Assignee: APPLIED BIOSYSTEMS, LLC
    Inventors: Patrick K. Martin, David A. Simpson
  • Altering the interface of hydrocarbon-coated surfaces
    Patent number: 9200191
    Abstract: Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or pipeline maintenance.
    Type: Grant
    Filed: November 19, 2013
    Date of Patent: December 1, 2015
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Edwin R Hendrickson, Scott Christopher Jackson, Abigail K Luckring, Michael P Perry
  • T7 RNA polymerase variants with enhanced thermostability
    Patent number: 9193959
    Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.
    Type: Grant
    Filed: March 23, 2011
    Date of Patent: November 24, 2015
    Assignee: Roche Diagnostics Operations, Inc.
    Inventors: Harald Sobek, Johann-Peter Thalhofer, Rainer Mueller, Manfred Schmidt, Michael Greif, Armin Ruf, Christian Rudolph
  • High fidelity DNA polymerase compositions and uses thereof
    Patent number: 9181534
    Abstract: The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme, where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 5?-3? exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: November 10, 2015
    Assignee: Agilent Technologies Inc.
    Inventors: Holly Hogrefe, Michael Borns, Joseph A. Sorge
  • Mutations in OAS1 genes
    Patent number: 9163222
    Abstract: Modified amino acid sequences of OAS1 proteins in non-human primates, and genes related thereto, are provided.
    Type: Grant
    Filed: November 14, 2012
    Date of Patent: October 20, 2015
    Assignee: Kineta Two, LLC
    Inventors: Shawn P Iadonato, Charles L Magness, P Campion Fellin, Christina A Scherer, Tory Hagen, Amy Olson
  • Compositions with polymerase activity
    Patent number: 9145550
    Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: September 29, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Peter B. Vander Horn, Yan Wang
  • Methods of using improved polymerases
    Patent number: 9139873
    Abstract: This invention provides for methods of sequencing and performing polymerase reactions using an improved generation of nucleic acid polymerases. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the processivity of the polymerase.
    Type: Grant
    Filed: June 21, 2013
    Date of Patent: September 22, 2015
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Yan Wang, Peter Vander Horn, Lei Xi
  • Method of removing nucleic acid contamination in reverse transcription and amplification reactions
    Patent number: 9133447
    Abstract: The invention provides methods of removing nucleic acid contamination from reverse transcription reactions and hot-start PCR, wherein said hot-start PCR is a barrier hot-start PCR set up and/or involves a hot-start DNA polymerase, which methods comprise use of a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA. The invention further provides a DNase that is substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 minutes, and that is substantially specific for double stranded DNA, nucleic acids encoding said DNase and kits or compositions comprising said DNase or said nucleic acid.
    Type: Grant
    Filed: September 25, 2013
    Date of Patent: September 15, 2015
    Assignee: Biotec Pharmacon ASA
    Inventors: Olav Lanes, Morten Elde, Dag Rune Gjellesvik
  • Enzymes resistant to photodamage
    Patent number: 9127259
    Abstract: Provided are compositions comprising modified DNA polymerases that exhibit improved photostability compared to the parental polymerases from which they were derived. Provided are methods for generating enzymes, such as DNA polymerases, with the aforementioned phenotype. Provided are methods of using polymerases with increased resistance to photodamage to make a DNA or to sequence a DNA template.
    Type: Grant
    Filed: March 30, 2009
    Date of Patent: September 8, 2015
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Keith Bjornson, Sonya Clark, Insil Park, Fred Christians
  • High fidelity thermostable ligase and uses thereof
    Patent number: 9127269
    Abstract: The present invention is directed to a mutant thermostable ligase having substantially higher fidelity than either T4 ligase or Thermus thermophilus ligase. The ligase of the present invention is a mutant of a wild-type thermostable ligase having a histidine adjacent a KXDG motif, where the mutant thermostable ligase has a mutation in its amino sequence where the histidine adjacent the KXDG motif in the wild-type thermostable ligase is replaced with an arginine, and wherein X is any amino acid. The DNA molecule encoding this enzyme as well as expression systems and host cells containing it are also disclosed. The thermostable ligase of the present invention is useful in carrying out a ligase detection reaction process and a ligase chain reaction process.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: September 8, 2015
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Francis Barany, Weiguo Cao, Jie Tong
  • Endoribonuclease compositions and methods of use thereof
    Patent number: 9115348
    Abstract: The present disclosure provides variant Csy4 endoribonucleases, nucleic acids encoding the variant Csy4 endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Csy4 endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell.
    Type: Grant
    Filed: November 7, 2012
    Date of Patent: August 25, 2015
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Rachel E. Haurwitz, Jennifer A. Doudna, Blake Wiedenheft, Martin Jinek