Abstract: Methods and compositions are provided for rapid detection of nucleic acid sequences. The method employs two reagent sets. The first set is a labelling set comprising: (1) a first nucleic acid sequence probe having an analyte complementary region and a first recognition sequence region and (2) a labelled sequence complementary to the first recognition sequence region. The second set is a capturing set comprising: (1) a second nucleic acid sequence probe having an analyte complementary region and a second recognition sequence region, (2) a specific binding pair member conjugated to a sequence complementary to the second recognition sequence, and (3) a separating means to which is bound a complementary specific binding pair member. The sample and probes are combined under annealing conditions, followed by addition of the other reagents, separation of the bound label from the supernatant and detection of the label in either phase.

Abstract: An antibody-based bioassay for enzyme-inducing chemicals is disclosed. The percent induction of an enzyme or class of related enzymes in living cells by an enzyme-inducing chemical in a sample is determined before and after the addition to the sample of an antibody to the chemical. The concentration of the chemical before and after the addition of the antibody is determined by comparison to the percent induction by known concentrations of the chemical. This permits the determination of the concentration of the chemical in the sample.

Abstract: This invention is a method for obtaining improved NMR images of the uterine cavity and fallopian tubes comprising imjecting a physiologically acceptable solution of a chelate of a parmagnetic material into the uterus under sufficient pressure to cause the solution to pass through the fallopian tubes and obtaining an enhanced NMR image of the tissue surrounding the fallopian tubes. The chelates and the injection solutions thereof do not include carbohydrate groups or other compounds which might support growth of Staphylococcus aureus or other pathological miroorganisms in the peritoneal cavity.Kits including contrast medium and non-metallic uterine balloon catheters suitable for use with NMRI procedures are also included within the scope of this invention.

Abstract: The compounds of the invention comprise the condensation product, as well as derivatives thereof, of two equivalents of a trimethyl cyclohexane-anhydride acid chloride derivative with one equivalent of an aromatic diamine. The scope of the invention includes the method of using the compounds of the invention as chelating agents for metals, metal ions or ions of metal complexes. In a preferred embodiment of the invention the binding moieties of the cyclohexane derivatives are rigidly held opposite each other, by restricting their rotation about the N-C aryl bonds, in order to more effectively bind the metals or the ions.

Abstract: This invention relates to a method for determination of cholinesterase activity, characterized by using, as a substrate, a choline derivative represented by the general formula (I): ##STR1## wherein X is a halogen atom; Y.sub.1 is a hydroxyl group as a substituent in the 2- or 5-position; and Y.sub.2 is a hydroxyl group as a substituent in the 3-position.The determination method of this invention permits easy and simple determination of cholinesterase activity, and is very useful as a determination method for clinical examinations for the purpose of determining cholinesterase in serum.

Abstract: Antibodies and antibody conjugates which have been modified by conjugation to, or exposure thereon, of glycoside residues that bind to the human hepatic asialoglycoprotein receptor clear rapidly from the circulation. Use of such modified antibodies and antibody conjugates for imaging and therapy of tumors and infectious lesions is advantageous when the antibodies are administered by a regional route, or when intravenous administration is accompanied by injection of a competitive hepatic lectin binding inhibitor to control the rate of clearance and optimize uptake by the target tissues.

Abstract: Labellable reagents for fluormetric assays comprise a cyclic condensation product of a .beta.-diketone, an aldehyde and an NH.sub.2 -bearing macromolecule, for example an antigen or antibody or a substance having an active group to which an antibody or antigen is linked. The reagents can chelate lanthanide metal ions such as Eu(II) and Tb(III) to form fluorescing complexes which can be used as labelled reagents for fluorometric assay of organic substances, for example antigens, antibodies and other substances occurring in body fluids.

Abstract: A method for determining the quantity of an antibody in a sample, the method having the steps of: (1) providing a labelled antigen to the antibody; (2) contacting the labelled antigen with the sample in solution to form a labelled antigen-antibody complex; (3) providing an agent for precipitating the complex; (4) mixing the solution containing the labelled antigen-antibody complex with the precipitating agent to produce a precipitate and a supernatant; the supernatant containing labelled antigen and the precipitate containing the labelled antigen-antibody complex and uncomplexed labelled antigen; and (5) measuring the quantity of label in the precipitate or the supernatant in a manner substantially independent of the amount of uncomplexed labelled antigen in the precipitate.

Abstract: A novel competitive assay for theophylline wherein caffeine-like (7-substituted) labeled conjugates are used to detect the presence and/or amount of theophylline present in a test sample. The use of such conjugates in a competitive assay for theophylline results in improved sensitivity of the assay method. Where the assay method is a nephelometric or turbidimetric inhibition immunoassay procedure, the assay was found to be less temperature dependent than prior art immunoassays.

Abstract: A clinical diagnostic method capable of rapidly detecting the presence of Mycoplasma pneumoniae in infected humans is taught. The method allows a proper course of therapy to be chosen within one day of presentation of the patient.

Abstract: A chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme and a nitrogen compound selected from the group consisting of ammonia and a water-soluble organic amine. The reaction of such process can be used in detection of nucleic acid hybrids, antibodies, antigens and peroxidase enzymes and in producing light.

Abstract: Peroxidatively active enzyme assays have a variety of clinical and industrial applications. Such assays are based on color development resulting from the addition of the appropriate substrate to the enzyme-containing medium. The stability of the said substrate and the extent of appearance of color are two serious limitations in enzyme assays. A method is disclosed herein for carrying out peroxidatively active enzyme assays when the enzyme is in free solution or in cell-bound form. Stable chemical compositions are also disclosed for use as substrates for carrying out such method.

Abstract: Polyiodothyronine analog conjugates to glucose-6-phosphate dehydrogenase (G6PDH) are provided which find use in the determination of polyiodothyronine compounds, particularly thyroxine, normally in physiological fluids, such as serum. The G6PDH enzyme activity can be substantially reduced when bound to antibodies specific to polyiodothyronine as compared to the free or unbound polyiodothyronine analog enzyme conjugates. The polyiodothyronine analog is conjugated to the G6PDH by a linking group having a chain one atom in length.

Abstract: Methods are provided for detecting and determining the amount in a sample of an antigenic determinant from an antigen receptor derived and released from a T cell or NK cell. Methods are also provided for detecting and determining the amount in a sample of an antigenic determinant from a complex of at least a portion of an antigen receptor derived from and released from a T cell or NK cell and a protein complex. These methods form the bases for methods of diagnosing and monitoring in a subject a disease characterized by the presence or an amount different from a normal subject of one of these antigenic determinants in a body fluid. A soluble antigen receptor or complex thereof derived from a T cell or a NK cell but free of such T cell or NK cell is also provided.

Abstract: Immunoassay methods and compositions are disclosed for the detection of analytes in fluid samples. The disclosure provides conjugates of analytes or reactants with polymerizable organic monomers. Specific binding reactions between reactants are detected by means of resporter/reactant conjugates. Free and specifically-bound reporter/reactant conjugates are separated by a polymerization reaction which renders the polymerized monomers insolule.

Abstract: The invention relates to a probe constituted by an oligonucleotide fragment of structure predetermined by the application envisaged, wherein at least a part of the adenine groups therein is replaced by modified adenine groups so that they can form three hydrogen bonds with the reactive groups of thymine or uracil. This probe is useful in the field of biological analyses and extractions.

Abstract: New 6,7-disubstituted-1-cyclopropyl-1,4-dihydro-4-oxo-1,8-naphthyridine-3-carb oxy lic acids of the formula (I) ##STR1## in which X represents halogen or nitro and A represents ##STR2## wherein R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are defined hereinbelow are disclosed as well as their usefulness as antibacterial agents.

Abstract: A secondary monoclonal antibody against a complex of a molecule of molecular weight less than 5000 and a binding protein against said molecule which secondary monoclonal antibody is not an antibody against molecule or against its binding protein.

Abstract: A class of new caphalosporin compounds (syn-isomer) is now provided, which is useful as antibacterial agent and is represented by the general formula (I) ##STR1## wherein R.sup.1 is an amino group or a protected amino group; R.sup.2 is a lower alkyl group, a carboxymethyl group or a protected carboxymethyl group; R.sup.3 is a hydrogen atom, a salt-forming cation or a carboxyl-protecting group; A is an unsubstituted or substituted phenyl group, an unsubstituted or substituted furyl group, an unsubstituted or substituted thiazolyl group or an unsubstituted or substituted 3-lower-alkylthiazolio group, and a pharmaceutically acceptable salt or ester thereof.

Abstract: New method for the detection of specific binding agents and their corresponding bindable substances by employing a label which is a latex particle which can be visually detected.