Abstract: Provided herein are improved expansion microscopy methods. Also provided herein are kits useful in expansion microscopy.

Abstract: The present invention relates to formulations of ADAMTS13 with enhanced or desirable properties. As such, the invention provides liquid and lyophilized formulations of ADAMTS13 that are suitable for pharmaceutical administration. Among other aspects, the present invention also provides methods of treating various diseases and conditions related to VWF and/or ADAMTS13 dysfunction in a subject. Also provided herein are kits comprising ADAMTS13 formulations useful for the treatment of various diseases and conditions.

Abstract: Provided herein are transmucosal and transdermal delivery systems comprising: at least one non-ionic surfactant; at least one polyol; and at least one active agent; and optionally further comprising at least one oil. In particular embodiments, the delivery system has an average particle size of from about 5 nm to about 200 nm.

Abstract: A method of analyzing the likelihood of weight regain in a subject who has reached a target weight by practicing a weight loss program is disclosed. The method comprises determining an amount or presence of at least one microbe and/or product thereof in the gut microbiome of the subject, wherein the amount of the at least one microbe is altered during a prior weight gain period of the subject to reach a level representative of an obese subject and further wherein the amount of the at least one microbe is retained at the level following the weight loss program, wherein the amount or presence of the at least one microbe or product thereof is predictive of weight regain.

Abstract: Methods of analyzing glycosylated biomolecules include the steps of producing a deglycosylation mixture of biomolecules deglycosylated by natural or synthetic enzymatic or chemical techniques; providing a reagent solution having a labeling reagent in a polar aprotic, non-nucleophilic organic solvent; and mixing the deglycosylation mixture with the reagent solution in an excess of labeling reagent to produce derivatized glycosylamines. The method steps can be carried out purposefully without depletion of protein matter. A quenching solution can be added to the reaction mixture so that the pH of the reaction mixture is shifted to above 10. The yield of derivatized glycosylamines can be in an amount of about 80 to about 100 mole percent of the reaction mixture with minimal overlabeling, less than 0.2 mole percent.

Abstract: Disclosed are compositions, methods, and systems for propagating microorganisms for fermentation.

Abstract: The present invention relates to a method for culturing microorganisms, in particular protists, carried out in such a way as to prevent the microorganisms from adhering to the walls of the reactors in which the culture is performed.

Abstract: A culture for the fermentative production of Massoia lactone comprising an Aureobasidium melanogenum species in a culture medium, wherein the Aureobasidium melanogenum species expresses no functional Aureobasidin A synthase gene mRNA when cultured, the culture medium including KH2PO4, Na2HPO4, (NH4)2SO4, MgSO4·7H2O and CaCl2·2H2O; at least two trace elements selected from the group consisting of Fe2+, Cu2+, Zn2+ and MoO42?; urea; and a carbon source selected from the group consisting of glucose, mannose, xylose and mixtures thereof, wherein the culture medium has a pH from 5.5 to 6.5.

Abstract: Disclosed are novel lysin polypeptides active against Gram-negative bacteria, particularly P. aeruginosa, pharmaceutical compositions containing them and methods for their use to treat Gram-negative bacterial infections and more generally to inhibit the growth, or reduce the population, or kill Gram-negative bacteria, including without limitation disrupting biofilms formed by such bacteria. Certain of the disclosed lysins have been modified in amino acid sequence compared to that of lysins by replacement of certain charged amino acids with noncharged amino acids and/or by fusion at the N- or C-terminus with antibacterial peptide sequences with or without an intervening linker.

Abstract: Herein disclosed are improved methods for treating stress disorders, such as Post-Traumatic Stress Disorder and perceived stress, by administration of acetylcholine release inhibitors, such as neurotoxic proteins (e.g., a botulinum toxin), to a patient at specific locations and amounts.

Abstract: A strain of Bifidobacterium longum subsp. infantis Y46 with fimbriae (BI_Y46) includes a unique flagella structure, exhibits strong abilities in utilizing 2?-fucosyllactose (2?-FL), galacto-oligosaccharides and short-chain galacto-oligosaccharides, possesses excellent probiotic characteristics with higher levels of extracellular polysaccharides and surface proteins than a strain of Bifidobacterium longum subsp. infantis M63 (BI_M63) and a strain of Bifidobacterium longum subsp. infantis ATCC 15697 (BI_15697), and further displays stronger ability to tolerate gastric acid and inhibitory ability of the cell-free supernatant to Escherichia coli ATCC 15922 than the BI_15697. Therefore, the BI_Y46 has excellent probiotic potential and can be used to develop probiotics, infant food, prebiotic products, and functional foods.

Abstract: A method of making a porous three-dimensional graphene mesh includes combining a graphene-containing material and a polymer having a plurality of hydroxyl groups in an alcohol solvent to form a mixture, adding a salt to the mixture, heating the mixture to form a gel, and washing the gel with water to remove the salt from the gel, leaving behind stable pores to form a scaffold. A three-dimensional porous graphene mesh includes a graphene-containing material and a polymer. The polymer is crosslinked with the graphene-containing material such that the Young's Modulus of the mesh is at least about 5 GPa.

Abstract: Compositions and methods of making a culture medium from a polymeric material are described herein. The method can include (a) depolymerizing and/or dispersing the polymeric material to obtain a depolymerized and/or dispersed residue, and (b) combining the depolymerized and/or dispersed residue with one or more adjuvants to form a culture medium. In some embodiments, the polymeric material can be a plastic article, such as a waste carpet material. Culture media prepared from the compositions and methods are also described. The culture media are suitable for culturing microorganisms including bacterium, algae, and fungus. The microorganisms can be used to produce a bioproduct such as a biopolymer, an enzyme, or a cellular metabolite.

Abstract: A granule for detecting glucose in pet urine, includes: 80-100 parts by weight of a filler, 30-50 parts by weight of water-absorbent material, 2-4 parts by weight of a bacteriostatic agent, 5-10 parts by weight of an adhesive, 1-2 parts by weight of glucose oxidase, 2-4 parts by weight of peroxidase, 2-6 parts by weight of an indicator.

Abstract: A method for the preparation of bioactive compound by culturing a Bacillus species containing 16S rRNA having nucleotide sequence that is at least 80% identical to the nucleic acid sequence of the 16S rRNA of B. endophyticus DS43 of SEQ ID NO: 1. Tryptone soya broth (TSB) is an effective growth medium for the production of the bioactive compound. A growth medium used in the method contains date syrup and/or molasses. The isolated fluorescent bioactive compound has biocidal activity against bacteria including Serratia marcescens, Staphylococcus aureus, and Escherichia coli.

Abstract: A topical sanitizing composition for restoring skin's natural balance of bacteria is provided. The topical sanitizing composition includes about 0.02 wt. % to 10.0 wt. % of an active ingredient, at least about 40 wt. % of one or more C1-6 alcohols; and water. The active ingredient comprises one or more of a probiotic, probiotic derivative, and a prebiotic.

Abstract: Disclosed are beneficial complexes comprising entomopathogenic nematodes (EPNs), binders, and external cargos, methods for preparing the same and systems comprising the same.

Abstract: The invention relates to methods for controlling mycotoxin production in field populations of heterothallic and homothallic filamentous fungi through application of biocontrol compositions comprising selected strains of the heterothallic and homothallic filamentous fungi, the selected strains having a lineage of low mycotoxin production or no mycotoxin production and having opposite mating types (heterothallic) or both mating types (homothallic) (e.g., MAT1-1, MATT-2).

Abstract: The invention relates to animal feeds and feed premixes containing synergistically effective amounts of betaine hydrochloride and a phytase.

Abstract: The present invention relates generally to growth of bacteria. More specifically the invention relates to a method for oxidative environment adaptation of anaerobic microorganisms and their use in developing new probiotics. In particular, the present invention provides a method for adaptation of anaerobic microorganisms and selection of more oxygen tolerant anaerobic microorganisms, said method comprising the steps of culturing said microorganisms with a stepwise dual induction of oxidative stress via applied voltage and oxygen diffusion, and a stepwise change of anti-oxidant/oxidized counterpart concentration ratio to adjust the redox state. New strains are also provided.