Abstract: A cell modified for obtaining increased proliferative capacity, decreased aging and enhanced regenerative capacity, its modification involving HOXB7 overexpression obtained using a gene vector that can insert the coding sequence into the cell, thereby affording increased protein production.
Abstract: The invention relates to the production and use of Cas-encoding sequences and vectors comprising these. Aspects of the invention provide products, vectors, delivery vehicles, uses and methods for producing Cas-encoding sequences in bacterial or archaeal cells.
Type:
Grant
Filed:
February 3, 2021
Date of Patent:
April 18, 2023
Assignee:
SNIPR BIOME APS
Inventors:
Virginia Martinez, Ruben Vazquez-Uribe, Adam Takos, Eric Van Der Helm, Jasper Clube
Abstract: A method of determining the latent HIV reservoir level in a subject includes obtaining a blood sample from an HIV+ subject, isolating CD4+ T cells from the biological sample, administering one or more HIV transcription inducing agents to the isolated CD4+ T cells, isolating RNA from the CD4+ T-cells that includes HIV env mRNA, producing a plurality of first amplicons from the from the isolated RNA using a first primer set that corresponds to an HIV genomic region encoding the HIV env protein, producing a plurality of second amplicons from the plurality of first amplicons using a second primer set, the second primer set including one or more adapter sequences and/or uniquely identifiable barcode sequences, and determining the nucleic acid sequences of the second amplicons, wherein the determined nucleic acid sequences in the sample are indicative of the amount of inducible cell-associated HIV env RNA in the sample and indicative of the latent HIV reservoir in the subject.
Type:
Grant
Filed:
September 27, 2018
Date of Patent:
April 11, 2023
Assignee:
CASE WESTERN RESERVE UNIVERSITY
Inventors:
Jonathan Karn, Miguel Quinones-Mateu, Curtis Dobrowolski
Abstract: Engineered CRISPR-Cas9 nucleases with altered and improved PAM specificities and their use in genomic engineering, epigenomic engineering, and genome targeting.
Abstract: The disclosure describes the effects of transcription mediated from a promoter on the transcription mediated by divergently coupled supercoiling-sensitive promoter. Transcription initiated from a promoter inhibits transcription mediated by a specific supercoiling-sensitive promoter that is divergently coupled to the promoter. A gyrase inhibitor relieves this inhibition and substantially increases the transcription mediated by the specific supercoiling-sensitive promoter that is divergently coupled to another promoter. Accordingly, the invention pertains to a method for identifying a compound as a gyrase inhibitor or not a gyrase inhibitor based on differential expression of genes under the control of divergently coupled promoters in the presence of the compound. Another embodiment of the invention provides an assay for identifying one or more compounds from a library of compounds as a gyrase inhibitor.
Type:
Grant
Filed:
June 15, 2018
Date of Patent:
March 28, 2023
Assignee:
THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
Abstract: The invention provides methods for determining the MSI status of a patient by liquid biopsy with sample preparation using hybrid capture and non-unique barcodes. In certain aspects, the invention provides a method of detecting microsatellite instability (MSI). The method includes obtaining cell-free DNA (cfDNA) from a sample of blood or plasma from a patient and sequencing portions of the cfDNA to obtain sequences of a plurality of tracts of nucleotide repeats in the cfDNA. A report is provided describing an MSI status in the patient when a distribution of lengths of the plurality of tracts has peaks that deviate significantly from peaks in a reference distribution.
Abstract: Methods and oligonucleotide reagents for diagnosing chikungunya virus and Zika virus infections are described. In particular, the invention relates to quantitative assays that can detect all lineages of chikungunya virus and Zika virus and distinguish chikungunya virus and Zika virus from each other as well as dengue virus and other arbovirus pathogens.
Type:
Grant
Filed:
January 14, 2017
Date of Patent:
February 28, 2023
Assignee:
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.
Type:
Grant
Filed:
August 6, 2018
Date of Patent:
February 28, 2023
Assignee:
10X GENOMICS, INC.
Inventors:
Benjamin Hindson, Christopher Hindson, Michael Schnall-Levin, Kevin Ness, Mirna Jarosz, Donald Masquelier, Serge Saxonov, Landon Merrill, Andrew Price, Paul Hardenbol, Yuan Li
Abstract: The present disclosure provides improved nucleic acid sequencing-by-synthesis (SBS) methods, related kits and reagents, and systems for performing such methods using such kits and reagents.
Type:
Grant
Filed:
March 6, 2018
Date of Patent:
February 28, 2023
Assignee:
SINGULAR GENOMICS SYSTEMS, INC.
Inventors:
Eli Glezer, Andrew Spaventa, David Barker
Abstract: Described herein are methods for determining a sequence of a region of interest from an mRNA molecule. Sequenced polynucleotides can include a barcode region, a homopolymer region (e.g., a poly-A region), and a target region associated with the mRNA molecule. According to some methods, the barcode region omits the same base present in the homopolymer region. According to some methods, extension of the primer used for sequencing is stalled within the homopolymer region. According to some methods, sequencing flow cycles and the different barcode regions of the polynucleotides configured are such that the primer is extended to the end of the barcode region across the plurality of polynucleotides before being extended into the homopolymer region. According to some methods, two primers or a cleavable primer is used to separately sequence the barcode region and the target region.
Abstract: The present invention generally relates to improved methods of assembly of two or more DNA fragments, methods of rapid ligation-independent cloning, and kits for rapid ligation-independent cloning and their uses.
Abstract: The present invention discloses a highly specific characteristic sequence, molecular specific labeled primer of Carya illinoensis variety Davis and applications thereof, and further discloses a method for rapidly identifying Carya illinoensis variety Davis. Wherein, the sequence of the molecular specific labeled primer is: upstream primer 5?-TCCTGAAAGCAGCCACAACA-3? (SEQ ID NO:3); downstream primer 5?-GACATGTGTACGAGGTGGTCA-3? (SEQ ID NO:4). The molecular specific labeled primer of the present invention can be used for early identification of the Carya illinoensis variety Davis rapidly and easily distinguishing it from other Carya illinoensis varieties, providing a strong technical support for the identification of Davis and planting resource protection. The method for rapid identification of the Carya illinoensis variety Davis is simple, rapid and accurate, and it is an irreplaceable molecular means for identifying Carya illinoensis variety by apparent characteristics.
Type:
Grant
Filed:
June 11, 2019
Date of Patent:
February 7, 2023
Assignee:
ZHEJIANG ACADEMY OF FORESTRY
Inventors:
Tangjun Zhu, Huazheng Peng, Qunying Jin, Hualin Ye
Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
Type:
Grant
Filed:
May 7, 2018
Date of Patent:
January 24, 2023
Assignee:
California Institute of Technology
Inventors:
Arjang Hassibi, Babak Hassibi, Haris Vikalo
Abstract: The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5? exonuclease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.
Type:
Grant
Filed:
February 24, 2020
Date of Patent:
January 3, 2023
Assignee:
Codex DNA, Inc.
Inventors:
Lei Young, Hamilton O. Smith, Daniel Glenn Gibson
Abstract: This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such methods.
Type:
Grant
Filed:
April 5, 2017
Date of Patent:
January 3, 2023
Assignee:
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
Inventors:
Salvatore A. E. Marras, Diana Vargas-Gold, Sanjay Tyagi, Fred Russell Kramer
Abstract: The present invention provides a method of predicting whether a pulmonary nodule in a subject is benign or non-small cell lung cancer, comprising obtaining the results of an assay that measures an expression level of miR205-5p in a plasma sample from the subject; obtaining the results of an assay that measures an expression level of miR126 in a plasma sample from the subject; obtaining the results of an assay that provides a size of the pulmonary nodule in the subject; and calculating a probability value based on the combination of the expression levels of miR205-5p and miR126, and the size of the pulmonary nodule, wherein if the probability value exceeds a specified threshold, the pulmonary nodule is predicted as non-small cell lung cancer.
Type:
Grant
Filed:
April 5, 2018
Date of Patent:
December 27, 2022
Assignees:
UNIVERSITY OF MARYLAND, BALTIMORE, THE UNITED STATES GOVERNMENT AS REPRESENTED BY THE DEPARTMENT OF VETERANS AFFAIRS
Abstract: Provided herein are a probe capable of simultaneously detecting a virus and treating virus-infected cells, a composition for detecting a virus, which comprises the probe, a composition for treating a virus, which comprises the probe, and a method of detecting a virus or treating a viral infection by using the same. According to the present disclosure, it is possible to simultaneously perform diagnosis by virus detection and treatment of virus-infected cells, and in particular, diagnosis and treatment may be simultaneously performed on various types of viruses by varying the type of molecular beacon, and thus may be usefully applied to virus diagnosis and treatment fields, which require rapid diagnosis and treatment, and the spread of viral infections may be effectively prevented. In addition, the probe of the present disclosure has excellent stability and excellent detection sensitivity, and thus enables the detection of even a very low amount of a target at the pmole level.
Type:
Grant
Filed:
September 26, 2018
Date of Patent:
December 27, 2022
Assignees:
Yonsei University Industry Foundation (Yonsei UIF), Korea University Research and Business Foundation, Sejong Campus
Inventors:
Seung Joo Haam, Hyun Ouk Kim, Byung Hoon Kang, Seung Min Han, Ji Sun Ki, Dae Sub Song, Woon Sung Na, Min Joo Yeom
Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
Type:
Grant
Filed:
May 7, 2018
Date of Patent:
December 13, 2022
Assignee:
California Institute of Technology
Inventors:
Arjang Hassibi, Babak Hassibi, Haris Vikalo