Patents Examined by Sandra Saucier
  • Preparation of a platelet/nucleated cell concentrate from bone marrow or blood
    Patent number: 7858296
    Abstract: The present invention is directed to methods and compositions regarding the preparation of an cell concentrate, such as, for example, an osteogenic cell concentrate, from a physiological solution, such as bone marrow aspirate, blood, or a mixture thereof. In specific embodiments, the invention provides methods and compositions utilizing two physiological solution-processing techniques, particularly in a point of care environment, wherein centrifugation is not employed.
    Type: Grant
    Filed: October 5, 2007
    Date of Patent: December 28, 2010
    Assignee: Smith & Nephew, Inc.
    Inventors: Samuel O. Sowemimo-Coker, Marcus Lee Scott, Marc Long, Ed Margerrison, Michael B. Cooper
  • Methods for prolonging survival of platelets using UDP-galactose
    Patent number: 7858295
    Abstract: The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis.
    Type: Grant
    Filed: June 29, 2007
    Date of Patent: December 28, 2010
    Assignee: Velico Medical, Inc.
    Inventors: Thomas P. Stossel, John H. Hartwig, Karin M. Hoffmeister, Henrik Clausen
  • Method of reducing glycolysis in platelet concentrates using L-carnitine, alkanoyl carnitines and salts thereof
    Patent number: 7851142
    Abstract: Cell membrane maintenance of red blood cells and platelet concentrates is improved by the addition of 1 mM-10 mM L-carnitine and derivatives. This improvement allows extension of the period of viability of packed red blood cells and platelet concentrations beyond current periods. Additionally, the materials so treated exhibit extended circulation half life upon transfusion to a patient. Improvements in membrane maintenance achieved by this method permit irradiation of sealed containers of blood products so as to substantially sterilize and destroy leukocytes in the same.
    Type: Grant
    Filed: June 28, 2007
    Date of Patent: December 14, 2010
    Assignee: Sigma-Tau Industrie Farmaceutiche Riunite S.p.A.
    Inventors: Secondo Dottori, Arduino Arduini
  • Method for preparing optically active compound
    Patent number: 7846701
    Abstract: According to the present invention, there is provided a method for preparing an optically active compound, characterized in that said method comprises permitting a mixture of optical isomers relative to the carbon atom in the ?-position in relation to the carbon atom bound to an esterified hydroxy group of an enol ester to hydrolyse either one optical isomer preferentially in the presence of an enzyme and allowing the carbonyl compound resulting from such hydrolysis to enrich the proportion of its isomer having either one configuration in the ?-position in relation to the carbonyl group or allowing the enol ester left non-hydrolyzed to enrich the proportion of its isomer having either one configuration on the carbon atom in the ?-position in relation to the carbon atom to which the esterified hydroxyl group bonds.
    Type: Grant
    Filed: October 31, 2005
    Date of Patent: December 7, 2010
    Assignee: Takasago International Corporation
    Inventors: Shigeru Tanaka, Shinya Watanabe, Ken-ichi Yamamoto, Hiroyuki Matsuda
  • Method for regenerating cartilage
    Patent number: 7838040
    Abstract: Disclosed is a method for regenerating articular cartilage in an animal comprising administering a therapeutically effective amount of a non-demineralized particulate articular cartilage having a distribution of particle sizes within the range of from about 60 microns to about 500 microns.
    Type: Grant
    Filed: November 28, 2006
    Date of Patent: November 23, 2010
    Inventor: Theodore Malinin
  • Method for producing antcin K, zhankuic acid A, zhankuic acid B or zhankuic acid C from the mycelium of
    Patent number: 7838264
    Abstract: The present invention provides a method for producing antcin K, zhankuic acid A, zhankuic acid B or zhankuic acid C from the mycelium of the fungus Antrodia cinnamomea by culturing the fungus on a gel medium, harvesting the mycelium and isolating the products by HPLC.
    Type: Grant
    Filed: June 18, 2008
    Date of Patent: November 23, 2010
    Assignee: Cojet Biotech Inc.
    Inventors: Yu-Lung Wang, Shu-Chun Chung
  • Length-dependent recoil separation of long molecules
    Patent number: 7833398
    Abstract: Separation of long molecules by length is obtained by forcing such molecules to traverse a boundary between a low free-energy region and a high free-energy region. In one embodiment, the high free-energy region is a dense pillar region or other structure formed on a semiconductor substrate. One or more membranes are used in further embodiments. The low free-energy region is a larger chamber formed adjacent the high free-energy region. A recoil phase allows longer molecules not fully driven into the high free-energy region to recoil into the low free-energy region. In a further variation, the high free-energy region is a membrane having nanoscale holes.
    Type: Grant
    Filed: November 9, 2007
    Date of Patent: November 16, 2010
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Harold G. Craighead, Stephen W. Turner
  • Drug testing with bio-artificial organ slices including for example those derived from liver
    Patent number: 7829325
    Abstract: A drug testing system using any number of bio-artificial organs, for example liver-slices based in a culture apparatus. The apparatus has a chamber with plasma and gas valves, animal organ slices being positioned securely in the chamber so as to maximize the surface area of organ slices exposed to the culture medium. Plasma is supplied to the chamber so that it rises to contact the organ slices, and is alternately removed from contacting the organ slices. Gas is supplied to the top of the chamber. The system also includes a reservoir for containing media entering and exiting the chamber. Methods are provided for assessing the toxicity of a drug or drug candidate.
    Type: Grant
    Filed: August 24, 2005
    Date of Patent: November 9, 2010
    Assignee: HepaHope, Inc.
    Inventor: Sung-Soo Park
  • Tissue preservation method with polyoxyethylene/polyoxypropylene copolymer
    Patent number: 7824847
    Abstract: Tissue preservation media comprising a polyoxyethylene/polyoxypropylene copolymer are used to preserve tissues and organs for storage and transplantation. In particular embodiments, the polyoxyethylene/polyoxypropylene copolymer is Pluronic F68 or FLOCOR (CRL-5861; purified poloxamer 188), and the medium is Steinhardt medium, polyoxyethylene/polyoxypropylene copolymer-supplemented Optisol GS or polyoxyethylene/polyoxypropylene copolymer-supplemented ViaSpan.
    Type: Grant
    Filed: January 17, 2008
    Date of Patent: November 2, 2010
    Assignee: Regents of the University of California
    Inventor: Richard A. Steinhardt
  • Preparation of enantiomerically enriched amines and amides by enzymatic resolution using a sulfonylacetic acid ester as acyl donor
    Patent number: 7824899
    Abstract: A process for the preparation of an enantiomerically enriched amine, is performed by a) cleaving a racemic mixture of a reaction product of i) a chiral amine and ii) an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; or b) reacting an amine with an acyl donor, in the presence of a hydrolase, to obtain a mixture of an enantiomerically enriched amide and an enantiomerically enriched amine; and c) separating the enantiomerically enriched amide from the enantiomerically enriched amine, wherein the acyl donor is a sulphonylacetic acid ester. The process (1) leads to high enantioselectivities, and (2) high reactivities, (3) is based on an acyl donor accessible in a simple manner, (4) is suitable for a large number of very different substrates and/or (5) is suitable for carrying out at high substrate concentrations.
    Type: Grant
    Filed: June 21, 2007
    Date of Patent: November 2, 2010
    Assignee: Evonik Degussa GmbH
    Inventors: Harald Groeger, Oliver May, Kai Rossen, Karlheinz Drauz
  • Method for removing viruses and leukocytes from blood using a surface comprising hydroxyl and polyethylene glycol groups
    Patent number: 7820371
    Abstract: It is intended to provide a method whereby a virus and leukocytes can be simultaneously eliminated from virus-containing blood and platelets can be recovered at a high yield, and a material and an apparatus therefor. A water-soluble carrier having surface which is capable of capturing a virus and leukocytes in blood but allows the permeation of platelets therethrough is brought into contact with virus-containing blood. Thus the virus and leukocytes can be simultaneously removed from the blood while platelets can be recovered at a high yield.
    Type: Grant
    Filed: July 2, 2007
    Date of Patent: October 26, 2010
    Assignee: Asahi Kasei Kuraray Medical Co., Ltd.
    Inventors: Hirokazu Onodera, Makoto Yoshida
  • Use of stabilized platelets as hemostatic agent
    Patent number: 7811558
    Abstract: The present invention provides compositions comprising freeze-dried platelets, microparticles, or both for use as a hemostat, such as for treating bleeding or injuries associated with bleeding. It also provides methods of treating injuries or wounds, and methods of causing blood to clot. Likewise, it provides methods of promoting healing of wounds or of healing wounds.
    Type: Grant
    Filed: August 12, 2005
    Date of Patent: October 12, 2010
    Assignee: Cellphire, Inc.
    Inventors: David Ho, Cindy S. Orser, Alan S. Rudolph
  • Enzymatic fluorometric assay for cAMP and adenylate cyclase and kits therefor
    Patent number: 7807367
    Abstract: The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents. Particularly, the present invention provides a method of determining CAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.
    Type: Grant
    Filed: April 19, 2007
    Date of Patent: October 5, 2010
    Assignee: Fuso Pharmaceutical Industries, Ltd.
    Inventor: Atsushi Sugiyama
  • Steady streaming particle traps
    Patent number: 7803599
    Abstract: A microfluidic fluid flow system (100) is disclosed having a fluid chamber or channel (150) with inlet and outlet ports (104, 106), allowing the fluid channel to be filled with a fluid. One or more flow obstructions or perturbances, such as cylinders (152), are provided in the channel. An oscillatory boundary condition is applied, for example, with a piezoelectric driver (130), that is selected to induce a conservative, low-intensity steady streaming flow in the channel. The low-intensity streaming flow produces distinct eddies that can be utilized, for example, for fluid-dynamically trapping or retaining particles (90) such as cells (92) at well defined locations in the channel. The system may be used to trap and study individual cells or for concentrating or filtering particles in a fluid.
    Type: Grant
    Filed: January 18, 2005
    Date of Patent: September 28, 2010
    Assignee: University of Washington
    Inventors: Barry R. Lutz, Daniel T. Schwartz
  • Medium supplement and animal cell culture medium
    Patent number: 7803616
    Abstract: An object of the present invention is to provide a medium supplement for animal cell culture and an animal cell culture medium. The present invention relates to a medium supplement for animal cell culture comprising sericin or a sericin derivative and an animal cell culture medium comprising at least said medium supplement and a basal medium composition.
    Type: Grant
    Filed: September 19, 2006
    Date of Patent: September 28, 2010
    Assignee: Seiren Kabushiki Kaisha
    Inventors: Masahiro Sasaki, Hideyuki Yamada, Katsue Osada, Satoshi Terada
  • Method for the production of flavor-active terpenes
    Patent number: 7803606
    Abstract: Disclosed is a method for producing flavor-active terpenes from terpene hydrocarbons. According to said method, a lyophilized mycel which is first rehydrated and is then mixed with the substrate is used preferably in a submerged culture in the framework of selective biotransformation with the aid of microorganisms. The inventive method, which can be carried out especially in an enantioselective, stereoselective, and/or regioselective manner, makes it possible to obtain terpenoid alcohols, epoxides, aldehydes, ketones, polyalcohols, carbonyls, and carbonyl alcohols with the aid of fusarium, pleutorus, penicillium, and chaetomium species, the obtained substances being isolated particularly from cellular components. Said method should be carried out above all in a stirred tank, surface reactor, or fixed bed reactor while preferably taking place in a two-phase system with reduced carbon source moieties.
    Type: Grant
    Filed: February 10, 2005
    Date of Patent: September 28, 2010
    Assignee: Maxens GmbH
    Inventors: Martin Müller, Kerstin Dirlam, Hans Henning Wenk, Ralf G. Berger, Ulrich Krings, Rüdiger Kaspera
  • Stemphones and production thereof
    Patent number: 7794991
    Abstract: The present invention relates to novel stemphones having enhancing effect of ?-lactam antibiotic used as an antibacterial agent, and a process for production thereof. The process is comprised of culturing microorganism belonging to genus Aspergillus and having ability to produce stemphones, the microorganism of which is Aspergillus sp. FKI-2136 NITE BP-83, accumulating the stemphones in the cultured mass, and isolating the stemphones from the cultured mass. Since the obtained stemphones have an action enhancing activity of ?-lactam antibiotic used as an antibacterial agent by combining with ?-lactam antibiotic, the stemphones are expected to be useful as the therapeutic agent for MRSA infection and infectious diseases caused by multi-drug resistant microorganisms including ?-lactam antibiotic resistance.
    Type: Grant
    Filed: October 3, 2005
    Date of Patent: September 14, 2010
    Assignee: The Kitasato Institute
    Inventors: Satoshi Omura, Hiroshi Tomoda, Rokuro Masuma
  • Method for the deacylation of lipopeptides
    Patent number: 7785826
    Abstract: The invention relates to a method for the enzymatic elimination of the N-acyl side chain from lipopeptides to form the corresponding nucleus, wherein the lipopeptide is prepared by fermentation, the lipopeptide being bound to the cells of the biomass, and the biomass is removed with the adhering lipopeptide, the biomass with the adhering lipopeptide is resuspended in an aqueous system, a suitable deacylase is added in dissolved or solid form to the suspension of the biomass, and the corresponding nucleus is formed, and the nucleus is optionally isolated and purified, wherein the lipopeptide obtained by fermentation is reacted after the end of the fermentation as cell-bound biomass without further purification directly with a deacylase, whereby the N-acyl chain linked via an amide linkage is eliminated.
    Type: Grant
    Filed: June 5, 2007
    Date of Patent: August 31, 2010
    Assignee: Sanofi-Aventis Deutschland GmbH
    Inventors: Eberhard Ehlers, Heinrich Decker, Sebastian Rissom, Guido Seidel, Reiner Olliger
  • Conjugates comprising a biodegradable polymer and uses therefor
    Patent number: 7785618
    Abstract: Biologically active agents covalently linked to a polymer. The polymer is preferably a biodegradable polymer are provided. The biologically active agent is preferably a protein, such as an extracellular soluble protein, e.g., an extracellular enzyme. The enzyme can be an apyrase, e.g., NTPDase. Conjugates of the invention can be used as therapeutics in subjects. For example, a conjugate comprising an apyrase can be used for treating and preventing thrombosis, atherosclerotic plaque complications and vascular disorders.
    Type: Grant
    Filed: August 20, 2004
    Date of Patent: August 31, 2010
    Inventors: David R. Elmaleh, Simon C. Robson, Mikhail L. Papisov
  • High pressure refolding of protein aggregates and inclusion bodies
    Patent number: 7767795
    Abstract: The present disclosure provides an effective method for the refolding of denatured proteins in solution so that properly folded, biologically active protein in solution is recovered in high yield. The refolding takes place at pressures between about 0.25 kbar to about 3.5 kbar, advantageously at about 1.5 kbar to about 3 kbar. Typically a chaotropic agent is present at a concentration which is not effective for denaturing protein at atmospheric pressure, and optionally, oxidation-reduction reagents can be incorporated in the refolding solution so that native intramolecular disulfide bonds can be formed where that is desired. The method is applicable to substantially all proteins, especially after solubilization and/or denaturation of insoluble protein aggregates, inclusion bodies, or abnormal oligomeric (soluble) aggregates.
    Type: Grant
    Filed: January 27, 2006
    Date of Patent: August 3, 2010
    Assignee: BaroFold Inc.
    Inventors: Theodore W. Randolph, John F. Carpenter, Richard St. John